Co-reporter:Sheng Wang, Yongbin Cao, Qin Zhang, Haibao Peng, Lei Liang, Qingguo Li, Shun Shen, Aimaier Tuerdi, Ye Xu, Sanjun Cai, and Wuli Yang
ACS Omega August 2017? Volume 2(Issue 8) pp:5170-5170
Publication Date(Web):August 30, 2017
DOI:10.1021/acsomega.7b00993
Finding a simple and effective strategy to eliminate tumor metastatic lymph nodes is highly desired in clinical tumor treatment. Herein, we reported a Chinese traditional ink (Hu-ink)-based treatment for photothermal therapy (PTT) of tumor metastatic lymph nodes. By simple dilution, stable Chinese traditional ink dispersion was obtained, which presents excellent photothermal effect because of its high absorption in near-infrared (NIR) region. Meanwhile, as revealed by staining and photoacoustic imaging, Hu-ink could transfer to nearby lymph nodes after directly injected into the primary tumors. Under the guidance of dual-modality mapping, the metastatic sentinel lymph nodes could be subsequently eliminated by NIR irradiation. The good biocompatibility of Hu-ink has also been verified by a series of experiments. Therefore, the Hu-ink-based treatment exhibits great potential for PTT of tumor metastatic lymph nodes in future clinical practice.Topics: Drug discovery and Drug delivery systems; Imaging;
Co-reporter:Xuejun Wang, Haichun Li, Xianping Liu, Ye Tian, Huishu Guo, Ting Jiang, Zimiao Luo, Kai Jin, Xinping Kuai, Yao Liu, Zhiqing Pang, Wuli Yang, Shun Shen
Biomaterials 2017 Volume 143(Volume 143) pp:
Publication Date(Web):1 October 2017
DOI:10.1016/j.biomaterials.2017.08.004
In this study, we reported a strategy to improve delivery efficiency of a long-circulation biomimetic photothermal nanoagent for enhanced photothermal therapy through selectively dilating tumor vasculature. By using a simply nanocoating technology, a biomimetic layer of natural red blood cell (RBC) membranes was camouflaged on the surface of photothermal polypyrrole nanoparticles (PPy@RBC NPs). The erythrocyte-mimicking PPy NPs inherited the immune evasion ability from natural RBC resulting in superior prolonged blood retention time. Additionally, excellent photothermal and photoacoustic imaging functionalities were all retained attributing to PPy NPs cores. To further improve the photothermal outcome, the endothelin A (ETA) receptor antagonist BQ123 was jointly employed to regulate tumor microenvironment. The BQ123 could induce tumor vascular relaxation and increase blood flow perfusion through modulating an ET-1/ETA transduction pathway and blocking the ETA receptor, whereas the vessel perfusion of normal tissues was not altered. Through our well-designed tactic, the concentration of biomimetic PPy NPs in tumor site was significantly improved when administered systematically. The study documented that the antitumor efficiency of biomimetic PPy NPs combined with specific antagonist BQ123 was particularly prominent and was superior to biomimetic PPy NPs (P < 0.05) and PEGylated PPy NPs with BQ123 (P < 0.01), showing that the greatly enhanced photothermal treatment could be achieved with low-dose administration of photothermal agents. Our findings would provide a promising procedure for other similar enhanced photothermal treatment by blocking ETA receptor to dramatically increase the delivery of biomimetic photothermal nanomaterials.
Co-reporter:Qin Jiang, Zimiao Luo, Yongzhi Men, Peng Yang, Haibao Peng, Ranran Guo, Ye Tian, Zhiqing Pang, Wuli Yang
Biomaterials 2017 Volume 143(Volume 143) pp:
Publication Date(Web):1 October 2017
DOI:10.1016/j.biomaterials.2017.07.027
Photothermal therapy (PTT) has represented a promising noninvasive approach for cancer treatment in recent years. However, there still remain challenges in developing non-toxic and biodegradable biomaterials with high photothermal efficiency in vivo. Herein, we explored natural melanin nanoparticles extracted from living cuttlefish as effective photothermal agents and developed red blood cell (RBC) membrane-camouflaged melanin (Melanin@RBC) nanoparticles as a platform for in vivo antitumor PTT. The as-obtained natural melanin nanoparticles demonstrated strong absorption at NIR region, higher photothermal conversion efficiency (∼40%) than synthesized melanin-like polydopamine nanoparticles (∼29%), as well as favorable biocompatibility and biodegradability. It was shown that RBC membrane coating on melanin nanoparticles retained their excellent photothermal property, enhanced their blood retention and effectively improved their accumulation at tumor sites. With the guidance of their inherited photoacoustic imaging capability, optimal accumulation of Melanin@RBC at tumors was achieved around 4 h post intravenous injection. Upon irradiation by an 808-nm laser, the developed Melanin@RBC nanoparticles exhibited significantly higher PTT efficacy than that of bare melanin nanoparticles in A549 tumor-bearing mice. Given that both melanin nanoparticles and RBC membrane are native biomaterials, the developed Melanin@RBC platform could have great potential in clinics for anticancer PTT.
Co-reporter:Yefei Tian;Zhipeng Ran
RSC Advances (2011-Present) 2017 vol. 7(Issue 69) pp:43839-43844
Publication Date(Web):2017/09/07
DOI:10.1039/C7RA07990G
Based on the copolycondensation of tetraethoxysilane and glucose, a facile one-pot approach is explored in this paper to prepare composite nanoparticles of silica/carbon dots (SCDs) on a large scale. Glucose, the carbon source for the carbon dots (CDs), is pre-packaged by copolycondensation with tetraethoxysilane in silica nanoparticles. It is likely that this creative route can reversibly modulate the excitation-dependent behavior of CD fluorescence. Compared with the character of the blue emission belonging to the carbon dots (CDs), which are illuminated under a UV lamp, SCDs suspended in water show bright tunable fluorescence from green to yellow. Moreover, the excitation dependence of the photoluminescence (PL) of the CDs is unprecedentedly restricted with a limitation of the CDs' growth in the silica nanoparticles, and afterwards the excitation dependence of PL can be fully recovered by the removal of the silica. In the solid state, the SCDs remain strongly luminescent and show temperature-sensitive PL. These findings pave the way for mechanistic studies on the excitation dependent fluorescence and applications of CDs in bioimaging and phosphor powders.
Co-reporter:Yefei Tian, Shanshan Bian and Wuli Yang
Polymer Chemistry 2016 vol. 7(Issue 10) pp:1913-1921
Publication Date(Web):05 Feb 2016
DOI:10.1039/C6PY00057F
Stimuli-responsive nanomaterials have promising prospects of application in controlled intracellular drug delivery. In this paper, we fabricated thermo- and redox-responsive biodegradable nanogels by precipitation copolymerization of 2-(2-methoxyethoxy)ethyl methacrylate, oligo(ethylene glycol)methacrylate, and a disulfide-containing crosslinker, N,N′-bis(acryloyl)cystamine. The poly(oligo(ethylene glycol)methacrylate) (POEGMA)-based nanogels exhibit a sharp volume collapse at their volume phase transition temperatures (VPTT), which are tunable in a wide temperature range. By incorporating disulfide bonds into polymer networks, the nanogels are endowed with an excellent redox-labile property that they can degrade efficiently into short polymer chains (Mw < 2000) in the presence of a reducing agent (glutathione or dithiothreitol). The anticancer drug (doxorubicin, DOX) loaded nanogels display a well-controlled release behavior, that is, low leakage of DOX under physiological conditions (only 8.1% in 48 h) and rapid and sufficient release of DOX in a reducing environment (92.2% in 48 h). Cell viability assays reveal that the blank nanogels have negligible cytotoxicity against normal cells (HEK-293T cells), while DOX-loaded nanogels present a significant inhibitive effect on tumor cells (HeLa cells).
Co-reporter:Ye Liu;Ye Tian;Yefei Tian;Yajun Wang
Advanced Materials 2015 Volume 27( Issue 44) pp:7156-7160
Publication Date(Web):
DOI:10.1002/adma.201503662
Co-reporter:Shanshan Bian, Jin Zheng, Xiaoling Tang, Deliang Yi, Yajun Wang, and Wuli Yang
Chemistry of Materials 2015 Volume 27(Issue 4) pp:1262
Publication Date(Web):January 23, 2015
DOI:10.1021/cm5042315
Monodisperse poly(vinylcaprolactam) (PVCL)-based capsules are prepared by precipitation polymerization of vinylcaprolactam (VCL) onto dimethyldiethoxysilane (DMDES) emulsion droplets and removal of the DMDES templates by ethanol. Polymer chains in the shells can be cross-linked during the polymerization by disulfide-containing cross-linker N,N′-bis(acryloyl) cystamine, which endows the capsules with an excellent redox-labile property. Versatility of this technique to prepare capsules with diverse components is demonstrated by the copolymerization of methacrylic acid (MAA) and VCL in the shell to prepare poly(vinylcaprolactam-co-methacrylic acid) (P(VCL-co-MAA)) capsules. The disulfide-bonded capsules can degrade efficiently into low molecular weight species (ca. 1200 Da) when the capsules are incubated with 10 mM glutathione (GSH) as the reducing agent. Delivery of the anticancer drug (doxorubicin, DOX) was also investigated in the P(VCL-co-MAA) capsules. The cumulative in vitro release of DOX-loaded capsules allows a relatively low DOX release at pH 7.4. However, a burst release (ca. 90% in 6 h) of DOX was observed in the presence of 10 mM GSH. Cell viability assays show that the P(VCL-co-MAA) capsules have negligible cytotoxicity to HeLa cancer cells. In comparison, DOX-loaded P(VCL-co-MAA) capsules cause significant cell death following internalization. The reported capsules represent a novel and versatile class of stimuli-responsive carriers for controlled drug delivery.
Co-reporter:Yang Wang, Jin Zheng, Yefei Tian and Wuli Yang
Journal of Materials Chemistry A 2015 vol. 3(Issue 28) pp:5824-5832
Publication Date(Web):16 Jun 2015
DOI:10.1039/C5TB00703H
To improve the biocompatibility and biodegradability of nanocarriers, well-defined poly(vinylcaprolactam)-based acid degradable nanogels were fabricated for drug delivery via precipitation polymerization in water, where synthetic ketal-based 2,2-dimethacroyloxy-1-ethoxypropane (DMAEP) acted as a cross-linker, and N-(2-hydroxypropyl)methacrylamide (HPMA) served as a co-monomer. Expectedly, we observed that the temperature and pH of the environment play important roles in the performance of the nanogels. The nanogels were reduced in size upon increasing the temperature and showed higher volume phase transition temperature (VPTT) with higher concentration of HPMA. With the incorporation of ketal linkages, the nanogels showed accelerated degradation profiles by lowering the pH and increasing temperature of the incubation medium. When used as nanocarriers of anticancer drug doxorubicin (DOX), compared to non-degradable nanogels with similar components, the acid-degradable nanogels displayed more effective drug controlled release behaviour, low drug leakage of DOX at neutral pH while rapid and sufficient release from the nanogels under acidic conditions. The results of the cytotoxicity and hemolysis assays further highlighted that the acid-degradable nanogel produced no hemolysin but showed excellent viability to normal cells, and the DOX-loaded nanogel exhibited higher proliferation inhibition against tumor cells.
Co-reporter:Rui Zheng, Sheng Wang, Ye Tian, Xinguo Jiang, Deliang Fu, Shun Shen, and Wuli Yang
ACS Applied Materials & Interfaces 2015 Volume 7(Issue 29) pp:15876
Publication Date(Web):July 7, 2015
DOI:10.1021/acsami.5b03201
Recently, photothermal therapy (PTT) that utilizes photothermal conversion (PTC) agents to ablate cancer under near-infrared (NIR) irradiation has attracted a growing amount of attention because of its excellent therapeutic efficacy and improved target selectivity. Therefore, exploring novel PTC agents with an outstanding photothermal effect is a current research focus. Herein, we reported a polydopamine-coated magnetic composite particle with an enhanced PTC effect, which was synthesized simply through coating polydopamine (PDA) on the surface of magnetic Fe3O4 particles. Compared with magnetic Fe3O4 particles and PDA nanospheres, the core–shell nanomaterials exhibited an increased NIR absorption, and thus, an enhanced photothermal effect was obtained. We demonstrated the in vitro and in vivo effects of the photothermal therapy using our composite particles and their ability as a contrast agent in the T2-weighted magnetic resonance imaging. These results indicated that the multifunctional composite particles with enhanced photothermal effect are superior to magnetic Fe3O4 particles and PDA nanospheres alone.Keywords: composite particles; Fe3O4; photothermal therapy; polydopamine; tumor;
Co-reporter:Jin Zheng, Yipeng Li, Yangfei Sun, Yongkun Yang, Yu Ding, Yao Lin, and Wuli Yang
ACS Applied Materials & Interfaces 2015 Volume 7(Issue 13) pp:7241
Publication Date(Web):March 18, 2015
DOI:10.1021/acsami.5b00313
While much effort has been made to prepare magnetic microspheres (MMs) with surface moieties that bind to affinity tags or fusion partners of interest in the recombinant proteins, it remains a challenge to develop a generic platform that is capable of incorporating a variety of capture ligands by a simple chemistry. Herein, we developed core–shell structured magnetic microspheres with a high magnetic susceptibility and a low nonspecific protein adsorption. Surface functionalization of these MMs with azide groups facilitates covalent attachment of alkynylated ligands on their surfaces by “click” chemistry and creates a versatile platform for selective purification and immobilization of recombinant proteins carrying corresponding affinity tags. The general applicability of the approach was demonstrated in incorporating four widely used affinity ligands with different reactive groups (−CHO, −SH, −COOH, and −NH2) onto the MMs platform for purification and immobilization of targeted proteins. The azide-functionalized MMs would be applicable for a variety of ligands and substrates that are amenable to alkynylation modification.Keywords: click chemistry; fusion protein; immobilization; magnetic microspheres; separation
Co-reporter:Ye Tian;Shun Shen;Jiachun Feng;Xingguo Jiang
Advanced Healthcare Materials 2015 Volume 4( Issue 7) pp:1009-1014
Publication Date(Web):
DOI:10.1002/adhm.201400787
Co-reporter:Shun Shen, Sheng Wang, Rui Zheng, Xiaoyan Zhu, Xinguo Jiang, Deliang Fu, Wuli Yang
Biomaterials 2015 39() pp: 67-74
Publication Date(Web):
DOI:10.1016/j.biomaterials.2014.10.064
Co-reporter:Ye Tian;Xuejiao Jiang;Xin Chen;Zhengzhong Shao
Advanced Materials 2014 Volume 26( Issue 43) pp:7393-7398
Publication Date(Web):
DOI:10.1002/adma.201403562
Co-reporter:Jin Zheng, Chongjun Ma, Yangfei Sun, Miaorong Pan, Li Li, Xiaojian Hu, and Wuli Yang
ACS Applied Materials & Interfaces 2014 Volume 6(Issue 5) pp:3568
Publication Date(Web):January 9, 2014
DOI:10.1021/am405773m
In this work, maltodextrin-modified magnetic microspheres Fe3O4@SiO2-Maltodextrin (Fe3O4@SiO2-MD) with uniform size and fine morphology were synthesized through a facile and low-cost method. As the maltodextrins on the surface of microspheres were combined with maltose binding proteins (MBP), the magnetic microspheres could be applied to enriching standard MBP fused proteins. Then, the application of Fe3O4@SiO2-MD in one-step purification and immobilization of MBP fused proteins was demonstrated. For the model protein we examined, Fe3O4@SiO2-MD showed excellent binding selectivity and capacity against other Escherichia coli proteins in the crude cell lysate. Additionally, the maltodextrin-modified magnetic microspheres can be recycled for several times without significant loss of binding capacity.Keywords: core−shell; Fe3O4; magnetic microspheres; maltodextrin; MBP; separation;
Co-reporter:Shaobo Ruan, Jun Qian, Shun Shen, Jiantao Chen, Jianhua Zhu, Xinguo Jiang, Qin He, Wuli Yang, and Huile Gao
Bioconjugate Chemistry 2014 Volume 25(Issue 12) pp:2252
Publication Date(Web):November 11, 2014
DOI:10.1021/bc500474p
Fluorescent carbonaceous nanodots (CDs) have attracted much attention due to their unique properties. However, their application in noninvasive imaging of diseased tissues was restricted by the short excitation/emission wavelengths and the low diseased tissue accumulation efficiency. In this study, CDs were prepared from glucose and glutamic acid with a particle size of 4 nm. Obvious emission could be observed at 600 to 700 nm when CDs were excited at around 500 nm. This property enabled CDs with capacity for deep tissue imaging with low background adsorption. Angiopep-2, a ligand which could target glioma cells, was anchored onto CDs after PEGylation. The product, An-PEG-CDs, could target C6 glioma cells with higher intensity than PEGylated CDs (PEG-CDs), and endosomes were involved in the uptake process. In vivo, An-PEG-CDs could accumulate in the glioma site at higher intensity, as the glioma/normal brain ratio for An-PEG-CDs was 1.73. The targeting effect of An-PEG-CDs was further demonstrated by receptor staining, which showed An-PEG-CDs colocalized well with the receptors expressed in glioma. In conclusion, An-PEG-CDs could be successfully used for noninvasive glioma imaging.
Co-reporter:Ye Liu, Ye Tian and Wuli Yang
RSC Advances 2014 vol. 4(Issue 102) pp:58758-58761
Publication Date(Web):03 Nov 2014
DOI:10.1039/C4RA13140A
In this study, carbon dots are found to be fabricated simultaneously during the typical hydrothermal synthesis process of magnetic colloidal nanocrystal clusters (MCNCs). Their existence in the solvent (ethylene glycol), on the surface and in the interior of MCNCs is also revealed.
Co-reporter:Zhipeng Ran and Wuli Yang
RSC Advances 2014 vol. 4(Issue 71) pp:37921-37927
Publication Date(Web):13 Aug 2014
DOI:10.1039/C4RA05897F
In this report, we present a ratiometric pH nanoprobe based on stable fluorescent colloidal silica composite nanoparticles encapsulating hydrophilic CdTe quantum dots. Quantum dots, owing to their fascinating optical properties, have been widely used for sensors and bioimaging. To avoid the inherent chemical instability and serious photoluminescence quenching of quantum dots, a facile electrostatic assembly method is developed to prepare sandwich-like silica/CdTe quantum dots/silica composite nanoparticles stabilized by mercaptopropyl trimethoxysilane (SQMS). This approach is of high efficiency, e.g., 98.9% of quantum dots are instantly adsorbed on the surface of silica nanospheres, and nearly 80% of the original fluorescence of the quantum dots is retained for SQMS while traditional silica coating processes caused dramatic quenching. Finally, the bright SQMS with remarkable stability is modified with pH-sensitive fluorescein isothiocyanate to fabricate a high-resolution pH ratiometric nanoprobe. Above all, SQMS shows a uniform sandwich-like structure, narrow size distribution, and stable fluorescence in strongly acidic and highly salted solutions, and the remarkable stability favours quantitative analyses and nanoprobes.
Co-reporter:Xuejiao Tian, Jia Guo, Ye Tian, Hongyan Tang and Wuli Yang
RSC Advances 2014 vol. 4(Issue 18) pp:9343-9348
Publication Date(Web):10 Dec 2013
DOI:10.1039/C3RA46082G
The influencing factors and mechanism of fluorescence enhancement or quenching in mesoporous nanocarriers consisting of gold nanorod cores and mesoporous silica shells (GNR@mSiO2) with controlled thicknesses were investigated. Mesoporous silica can act as a tool to change the distance between the fluorophores and GNRs as well as a vehicle to load the fluorescence molecules in this system. We found that the distance between the GNRs’ surface and the fluorophores, the distribution of fluorophores in mesoporous silica and the kind of fluorophore used were the three main reasons for the fluorescence change. The shell-thickness-dependent fluorescence enhancement of doxorubicin (DOX)-containing GNR@mSiO2 was observed. The maximum metal-enhanced fluorescence (MEF) was obtained when GNR@mSiO2–DOX had the thinnest mesoporous silica shell. Moreover, changing the concentration ratio between GNR@mSiO2 and DOX resulted in different fluorescence enhancement factors. Upon combination of GNR@mSiO2 with other fluorophores, such as hematoporphyrin dihydrochloride (HP) and rhodamine 6G (R6G), the fluorescence enhancement phenomenon was also observed. On the other hand, we found that the fluorescence enhancement factor was reduced when the emission wavelength of the fluorophores was generally close to the surface plasmon resonance wavelength of the gold nanorods. This mechanism was confirmed by fluorescence quenching on fluorescein isothiocyanate (FITC)-containing GNR@mSiO2.
Co-reporter:Shanshan Bian;Jin Zheng
Journal of Polymer Science Part A: Polymer Chemistry 2014 Volume 52( Issue 12) pp:1676-1685
Publication Date(Web):
DOI:10.1002/pola.27165
ABSTRACT
The synthesis and characterization of a new photocleavable crosslinker is presented here. Dual stimuli-responsive P(VCL-co-NHMA) microgels were prepared by precipitation polymerization of vinylcaprolactam (VCL) with N-hydroxymethyl acrylamide (NHMA) and the new crosslinker. The microgels had distinct temperature sensitivity as observed in the case of PVCL-based particles and their volume phase transition temperature (VPTT) shifted to higher temperature with increasing NHMA content. Photolytic degradation experiments were investigated by irradiation with UV light, which led to microgel disintegration caused by cleavage of the photolabile crosslinking points. The degradation behavior of the microgels was conducted with respect to degradation rates by means of the relative turbidity changes. Hence, the microgels could totally degrade into short linear polymers by UV light, thus representing a great potential as new light and temperature dual responsive nanoscale materials. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 1676–1685
Co-reporter:Baisong Chang, Dan Chen, Yang Wang, Yanzuo Chen, Yunfeng Jiao, Xianyi Sha, and Wuli Yang
Chemistry of Materials 2013 Volume 25(Issue 4) pp:574
Publication Date(Web):January 30, 2013
DOI:10.1021/cm3037197
The design of bioresponsive controlled drug delivery systems is a promising approach in cancer therapy, but it still is a major challenge capable of optimum therapeutic efficacy, i.e. no premature drug leakage in blood circulation while having a rapid and complete release in tumor tissues. In this work, a kind of PEGylated core/shell structured composite nanoparticle was developed via precipitation polymerization, where a disulfide-cross-linked poly(N-vinylcaprolactam-co-methacrylic acid) (P(VCL-s-s-MAA)) polymer shell was created to act as sheddable thermo/pH-sensitive gatekeepers, and a carboxylic acid modified mesoporous silica nanoparticles (MSN-COOH) core was applicable as an accessible reservoir to encapsulate high drug doses. At physiological conditions, the P(VCL-s-s-MAA)-PEG shell underwent a distinct transition from a swollen state in pH 7.4 to a collapsed state in pH 5.0. Though sufficiently stable in water, composite nanoparticles were prone to fast dissociation and rupture when subjecting to 10 mM glutathione (GSH), due to the shedding of polymer walls through reductive cleavage of intermediate disulfide bonds, so that the polymer shell was active in moderating the diffusion of embedded drugs in-and-out of MSN channels. The cumulative in vitro release of DOX-loaded composite nanoparticles allowed a low trace of DOX diffusion below volume phase transition temperature (VPTT) and a significant release rate above its VPTT, while the most rapid and perfect release was achieved under a reductive environment (pH 6.5 and 10 mM GSH), mimicking that of intracellular cytosol compartments. The in vitro cell assay of blank carriers to normal cells indicated that the composite nanoparticles were suitable as drug carriers, but DOX-loaded carriers had a similar intensive toxicity to cancer cells compared with free DOX. Therefore, these stimuli-responsive composite nanoparticles with a reductively sheddable and thermo/pH-responsive polymer shell gate could, in principle, be applied for in vivo cancer therapy, and synergistic drug delivery can be accomplished “just in time” in a precise event over the location.Keywords: controlled drug release; core/shell; MSN; shell-sheddable; thermo/pH-responsive;
Co-reporter:Miaorong Pan, Yangfei Sun, Jin Zheng, and Wuli Yang
ACS Applied Materials & Interfaces 2013 Volume 5(Issue 17) pp:8351
Publication Date(Web):August 8, 2013
DOI:10.1021/am401285x
In this work, core–shell–shell-structured boronic acid-functionalized magnetic composite microspheres Fe3O4@SiO2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (Fe3O4@SiO2@P(MMA-co-VPBA)) with a uniform size and fine morphology were synthesized. Here, Fe3O4 magnetic particles were prepared by a solvothermal reaction, whereas the Fe3O4@SiO2 microspheres with a core–shell structure were obtained by a sol–gel process. 3-(Trimethoxysilyl) propyl methacrylate (MPS)-modified Fe3O4@SiO2 was used as the seed in the emulsion polymerization of MMA and VPBA to form the core–shell–shell-structured magnetic composite microspheres. As the boronic acid groups on the surface of Fe3O4@SiO2@P(MMA-co-VPBA) could form tight yet reversible covalent bonds with the cis-1,2-diols groups of glycoproteins, the magnetic composite microspheres were applied to enrich a standard glycoprotein, horseradish peroxidase (HRP), and the results demonstrated that the composite microspheres have a higher affinity for the glycoproteins in the presence of the nonglycoprotein bovine serum albumin (BSA) over HRP. Additionally, different monomer mole ratios of MMA/VPBA were studied, and the results implied that using MMA as the major monomer could reduce the amount of VPBA with a similar glycoprotein enrichment efficiency but a lower cost.Keywords: boronic acid; composite microspheres; core−shell; enrichment; glycoprotein; magnetic particles;
Co-reporter:Yang Sun;Zhipeng Ran;Hongyan Tang;Yong Li;Wenshuang Song;Qingguang Ren;Jilie Kong
Chinese Journal of Chemistry 2013 Volume 31( Issue 6) pp:787-793
Publication Date(Web):
DOI:10.1002/cjoc.201300113
Abstract
Mesoporous silica nanoparticles (MSN) were coated by pH-responsive polymer chitosan-poly (methacrylic acid) (CS-PMAA). This nano drug delivery system showed good application prospects and the polymer-coated microspheres were promising site-specific anticancer drug delivery carriers in biomedical field. A continuous detection of pH-responsive drug delivery system in cells in situ, utilizing MSN/CS-PMAA composite microspheres, was proposed. Two kinds of different cell lines, tumor cell line (Hela) and normal somatic cells (293T), were used to investigate the behaviours of the drug loaded system in the cells. Conclusions could be drawn from the fluorescent images obtained by confocal laser scanning microscopy (CLSM), modified drug-loaded microspheres (MSN/CS-PMAA) were ingested into cells more easily, the uptake of DOX@FITC-MSN/CS-PMAA by HeLa/293T cells were performed at pH 7.4/pH 6.8, DOX was released during the ingestion process, fluorescence intensity decreased with time because of efflux transport and photo-bleaching. Fluoresence detection by flow cytometry was performed as comparison. The continuous fluorescent observation in situ could be widely used in the pH-responsive releasing process of drug delivery system in the cells.
Co-reporter:Shun Shen, Hongyan Tang, Xiaotong Zhang, Jinfeng Ren, Zhiqing Pang, Dangge Wang, Huile Gao, Yong Qian, Xinguo Jiang, Wuli Yang
Biomaterials 2013 34(12) pp: 3150-3158
Publication Date(Web):
DOI:10.1016/j.biomaterials.2013.01.051
Co-reporter:Yunfeng Jiao;Yangfei Sun;Dr. Baisong Chang; Daru Lu; Wuli Yang
Chemistry - A European Journal 2013 Volume 19( Issue 45) pp:15410-15420
Publication Date(Web):
DOI:10.1002/chem.201301060
Abstract
A controlled drug-delivery system has been developed based on mesoporous silica nanoparticles that deliver anticancer drugs into cancer cells with minimized side effects. The copolymer of two oligo(ethylene glycol) macromonomers cross-linked by the disulfide linker N,N′-bis(acryloyl)cystamine is used to cap hollow mesoporous silica nanoparticles (HMSNs) to form a core/shell structure. The HMSN core is applied as a drug storage unit for its high drug loading capability, whereas the polymer shell is employed as a switch owing to its redox/temperature dual responses. The release behavior in vitro of doxorubicin demonstrated that the loaded drugs could be released rapidly at higher temperature or in the presence of glutathione (GSH). Thus, the dual-stimulus polymer shell exhibiting a volume phase transition temperature higher than 37 °C can effectively avoid drug leakage in the bloodstream owing to the swollen state of the shell. Once internalized into cells, the carriers shed the polymer shell because of cleavage of the disulfide bonds by GSH, which results in the release of the loaded drugs in cytosol. This work may prove to be a significant development in on-demand drug release systems for cancer therapy.
Co-reporter:Yang Wang, Jinshan Nie, Baisong Chang, Yangfei Sun, and Wuli Yang
Biomacromolecules 2013 Volume 14(Issue 9) pp:
Publication Date(Web):August 2, 2013
DOI:10.1021/bm401131w
Poly(vinylcaprolactam) (PVCL)-based biodegradable microgels were prepared for the biomedical application as drug delivery system via precipitation polymerization, where N,N-bis(acryloyl) cystamine (BAC) served as cross-linker, methacrylic acid (MAA) and polyethylene glycol (PEG) methyl ether methacrylate acted as comonomers. The microgels with excellent stability had distinct temperature sensitivity as largely observed in the case of PVCL-based particles and their volume phase transition temperature (VPTT) shifted to higher temperature with increasing MAA content and ambient pH. In the presence of reducing agent glutathione (GSH) or dithiothreitol (DTT), the microgels could be degraded into individual linear polymer chains by the cleavage of the disulfide linkages coming from the cross-linker BAC. The microgels could effectively encapsulate Doxorubicin (DOX) inside and presented stimuli-triggered drug release in acidic or reducing environment. The results of the cytotoxicity assays further demonstrated that the blank microgels were nontoxic to normal cells while DOX-loaded microgels presented efficient antitumor activity to HeLa cells.
Co-reporter:Yunfeng Jiao, Jia Guo, Shun Shen, Baisong Chang, Yahong Zhang, Xinguo Jiang and Wuli Yang
Journal of Materials Chemistry A 2012 vol. 22(Issue 34) pp:17636-17643
Publication Date(Web):10 Jul 2012
DOI:10.1039/C2JM31821K
By employing poly (tert-butylacrylate) (PTBA) nanospheres as the dissolvable core templates, we develop a new method to synthesize hollow mesoporous silica nanoparticles (HMSN). Both the PTBA core and the structure-directing surfactant, cetyltrimethylammonium bromide (CTAB), can be easily and synchronously removed through solvent extraction in ethanol, which ensures the complete structure and ideal dispersibility of the products compared with the previous template synthesis that often needs calcination to remove the templates. Given that hollow core diameter and shell thickness are the key properties of HMSN, the hollow core diameter and shell thickness can be tailored precisely. In addition, as novel inorganic nanomaterials with a tuned structure, HMSN show notable biocompatibility and efficient doxorubicin (DOX) loading. In in vitro tests, the release rate of DOX-loaded HMSN exhibit a surprising shell-thickness-dependent and a pH responsive drug release character, suggesting that HMSN are a very promising drug delivery system for shell-thickness-controlled drug release. The results of intracellular tracking and cytotoxicity assays further demonstrate the potential and efficiency of HMSN as a drug delivery system.
Co-reporter:Hongyan Tang, Shun Shen, Jia Guo, Baisong Chang, Xinguo Jiang and Wuli Yang
Journal of Materials Chemistry A 2012 vol. 22(Issue 31) pp:16095-16103
Publication Date(Web):13 Jun 2012
DOI:10.1039/C2JM32599C
Novel composite nanoparticles based on poly(N-isopropylacrylamide-co-N-hydroxymethyl acrylamide) (P(NIPAM-co-NHMA)) layer coated gold nanorod@mesoporous silica (GNR@mSiO2) has been successfully synthesized by precipitation polymerization. The composite nanoparticles exhibited a thermo/near-infrared (NIR) light sensitivity. The volume phase transition temperature (VPTT) could be precisely regulated by the content of NHMA in monomers and an excellent photothermal conversion effect was expressed due to the surface plasmon resonance of gold nanorods in the composite nanoparticles. Doxorubicin hydrochloride (DOX) was applied as a model drug and the drug storage/release behavior was investigated. The results demonstrated that DOX could be effectively loaded into the composite nanoparticles with 21% loading capacity. The cumulative release of DOX-loaded composite nanoparticles was temperature dependent and the release rate was significantly enhanced above the VPTT. Therefore, the composite nanoparticles applied as a drug delivery system could reduce the side effect of DOX to normal tissues as only a small fraction of DOX was released from the composite nanoparticles at 37 °C. In addition, the DOX-loaded composite nanoparticles demonstrated a synergistic effect, the therapeutic efficacy was improved significantly by the combination of photothermal therapy and traditional chemotherapy with low composite nanoparticle concentration and short laser irradiation time in an in vitro study.
Co-reporter:Baisong Chang, Xurui Zhang, Jia Guo, Yang Sun, Hongyan Tang, Qingguang Ren, Wuli Yang
Journal of Colloid and Interface Science 2012 Volume 377(Issue 1) pp:64-75
Publication Date(Web):1 July 2012
DOI:10.1016/j.jcis.2012.03.082
A general and facile strategy was developed to coat hydrophilic inorganic nanoparticles directly with mesoporous silica nanoparticles (MSNs). The cationic surfactant of cetyltrimethylammonium bromide (CTAB) was adsorbed to various negatively charged CdTe quantum dots, Fe3O4 nanocrystals or Au nanoparticles, introducing the bilayer of CTAB overcoating with positive charge. The subsequent sol–gel reaction of TEOS with the basic catalyst resulted in uniform nanocomposites. The concentration of CTAB and NH4OH in the recipe strongly influenced the number of inorganic nanoparticles in the nanocomposites and the homogeneity of MSNs shell. One dimensional Au nanorods and larger size of solid SiO2 nanoparticles were also able to coat with MSNs using a similar synthetic procedure. The proposed method was greatly simplified without the help of any mediators or silane coupling agents and excellent mesostructural performance was readily achieved. Compared to the methods known from the literatures for the coating of hydrophobic nanoparticles, this efficient way is especially useful for trapping different hydrophilic nanoparticles with arbitrary sizes and shapes into MSNs. These highly versatile multifunctional nanocomposites, together with the pH-responsible drug release behaviors, non-toxicity to normal cells and ease of uptake into cancer cells, are expected to be utilized as drug delivery system for simultaneous imaging and therapeutic applications.Graphical abstractHighlights► A one-pot strategy was developed to coat hydrophilic nanoparticles with MSNs. ► The efficient way is useful for trapping various hydrophilic nanoparticles. ► The versatile nanocomposites have a good biocompatibility. ► Multifunctional nanocomposites are expected to be useful in cancer therapy/imaging.
Co-reporter:Xurui Zhang;Lixin Wang;Jia Guo
Chinese Journal of Chemistry 2012 Volume 30( Issue 5) pp:1031-1039
Publication Date(Web):
DOI:10.1002/cjoc.201100672
Abstract
The improved properties of CdTe nanocrystals (NCs) synthesized by hydrothermal method were introduced. The experimental results indicated that the NCs properties could be dramatically influenced by means of changing Cd-to-Te molar ratio (the molar ratio of CdCl2 and NaHTe in the precursor) of the MPA-capped CdTe NCs. With the increase of the ratio from 2:1 to 10:1, the formation time of near-infrared-emitting CdTe NCs was shortened. In particular, high Cd-to-Te molar ratio brought about MPA-capped CdTe NCs of superior radical oxidation-resistance and photostability. As a result, the optimum ratio was found to be 8:1 or 10:1 in the study in order to efficiently attain stable, water-dispersed CdTe NCs.
Co-reporter:Jie Chen;Jia Guo;Baisong Chang
Colloid and Polymer Science 2012 Volume 290( Issue 6) pp:517-524
Publication Date(Web):2012 April
DOI:10.1007/s00396-011-2574-x
Here we reported the synthesis of polyethylene glycol 2000 monomethyl ether (PEG)ylated hyperbranched poly (amido amine) (h-PAMAM-g-PEG) and the study of an elaborate control over the structure transition by solvents. The double hydrophilic hyperbranched copolymers could form micelles with h-PAMAM core and solvophilic PEG shell in tetrahydrofuran (THF). It was found that the micellization stage was prolonged if more PEG chains were anchored onto h-PAMAM cores. After cross-linking the h-PAMAM cores, well-dispersed hollow spheres were obtained when the micelles were transferred into water from THF. More grafted PEG chains on h-PAMAM may prohibit the creation of a hollow cage upon the swelling of the hydrophilic h-PAMAM cores. Such engineered hollow spheres also retained the pH-sensitive fluorescence characteristic, identical with the luminescent behavior of the free h-PAMAM molecules. H-PAMAM-g-PEG hollow spheres with pH-sensitive fluorescence have a potential application as a drug delivery vehicle for chemotherapy.
Co-reporter:Baisong Chang, Xianyi Sha, Jia Guo, Yunfeng Jiao, Changchun Wang and Wuli Yang
Journal of Materials Chemistry A 2011 vol. 21(Issue 25) pp:9239-9247
Publication Date(Web):23 May 2011
DOI:10.1039/C1JM10631G
In this paper, a kind of core–shell composite microsphere was prepared based on poly(N-isopropylacrylamide-co-methacrylic acid) (P(NIPAM-co-MAA)) coated magnetic mesoporous silica nanoparticles (M-MSN) via precipitation polymerization. The composite microsphere presented a thermo/pH-coupling sensitivity and the volume phase transition could be precisely regulated by the molar ratio of MAA to NIPAM or the concentration of NaCl. At physiological conditions (37 °C, 0.15 M NaCl), the P(NIPAM-co-MAA) shell underwent a distinct transition from a swollen state in pH 7.4 to a collapsed state in pH 5.0, so that the polymer shell was active in moderating the diffusion of embedded drugs in-and-out of the pore channels of MSN. Doxorubicin hydrochloride (DOX) was applied as a model drug and the behaviors of drug storage/release were investigated. The drug loaded behavior was pH-dependent, and the composite microsphere had a drug embed efficiency of about 91.3% under alkaline conditions. The cumulative in vitro release of the DOX-loaded composite microsphere showed a low level of leakage below the volume phase transition temperature (VPTT) and was significantly enhanced above its VPTT, exhibiting an apparent thermo/pH-response controlled drug release. The cytotoxicity assay of a blank carrier to normal cells indicated that the composite microspheres were suitable as drug carriers, while the DOX-loaded composite microspheres had a similar cytotoxicity to HeLa cells compared with free DOX. Therefore, the thermo/pH-sensitive composite microsphere could, in principle, be used for in vivo cancer therapy with a low premature drug release during blood circulation whilst having a rapid release upon reaching tumor tissues.
Co-reporter:Baisong Chang, Jia Guo, Congying Liu, Ji Qian and Wuli Yang
Journal of Materials Chemistry A 2010 vol. 20(Issue 44) pp:9941-9947
Publication Date(Web):24 Sep 2010
DOI:10.1039/C0JM01237H
The modified sol–gel approach to the synthesis of well-structured magnetic mesoporous silica nanoparticles (M-MSNs) was described, which comprised magnetic nanoparticles resided within the mesoporous nanoparticles. A diversity of surface modification was subjected to systematic investigation using organic silanes, eventually resulting in the decoration with the carboxyl (–COOH), methyl phosphonate (–PO3−), amino (–NH2) and phenyl (–Ph) groups on the surface of M-MSNs. The careful characterizations demonstrated that the modified M-MSNs displayed the specifically charged surfaces and differently porous characters, yet without showing any influence on the shape and size. To exploit their potential in cancer treatment, we extensively studied the drug loading capacity and sustained release behaviour of the modified M-MSNs for representative drugs. The hydrophilically modified M-MSNs with –COOH and –PO3− were beneficial for loading the water-soluble doxorubicin hydrochloride (DOX) through electrostatic attraction. The results demonstrated that M-MSNs-PO3− achieved a higher loading content and M-MSNs-COOH presented a distinct pH-responsible release behavior. On the other hand, M-MSNs-Ph displayed a controlled release rate in a short term via the weakened hydrogen bonding interaction. The cytotoxicity of modified M-MSNs to normal cells and macrophage uptake indicated that the modified M-MSNs were suitable as drug carriers. These mesoporous nanoparticles were non-toxicity to HeLa cells, while the drug-loaded nanoparticles apparently led to the unambiguous cytotoxicity as a result of the sustained release of drugs. These results have an important implication that the modified M-MSNs are promising as platforms for storing the hydrophilic or hydrophobic anticancer drugs for tumour therapy.
Co-reporter:Congying Liu, Jia Guo, Wuli Yang, Jianhua Hu, Changchun Wang and Shoukuan Fu
Journal of Materials Chemistry A 2009 vol. 19(Issue 27) pp:4764-4770
Publication Date(Web):27 May 2009
DOI:10.1039/B902985K
A controlled drug release system was designed based on the combination of three advantages into one entity, which was composed of Fe3O4 magnetic nanoparticle as the core, mesoporous silica as the sandwiched layer, and thermo-sensitive P(NIPAM-co-NHMA) copolymer as the outer shell. The hydrophilic comonomer content affected the volume phase-transition temperature (VPTT) of this composite microsphere and the behavior of the temperature-triggered drug release. Zn(II) phthalocyanine tetrasulfonic acid (ZnPcS4), a well-known photodynamic therapy (PDT) drug, was used as a model drug to assess the release system. The results demonstrated that the drug release behavior was dependent on the temperature and had a close correlation with the VPTT. Above the VPTT, the drug release rate was much faster than that below the VPTT, which showed a great potential application in tumor therapy.
Co-reporter:Yi Shi, Yahong Zhang, Wuli Yang and Yi Tang
Chemical Communications 2009 (Issue 4) pp:442-444
Publication Date(Web):19 Nov 2008
DOI:10.1039/B814095B
A series of multiradiate calcium phosphate patterns have been observed by a gradating chitosan-polyaspartatic acid (PAsp) system, and their morphology evolution reveals the effect of chitosan and PAsp on the interfacial biomineralization.
Co-reporter:Chunxu Wang, Lixin Wang, Wuli Yang
Journal of Colloid and Interface Science 2009 Volume 333(Issue 2) pp:749-756
Publication Date(Web):15 May 2009
DOI:10.1016/j.jcis.2009.02.008
In this paper, a simple approach was presented to prepare functional inorganic/organic composite microspheres in which the inorganic nanoparticles were encapsulated into the polyelectrolyte complexes between chitosan (CS) and poly(aspartic acid) sodium salt (PAsp). The magnetic microspheres were prepared via electrostatic interaction between the citrate groups stabilizing Fe3O4 nanoparticles and the amino groups of CS. Being taken as a kind of special polyelectrolyte with high molecular weight, the inorganic nanoparticles presented strong interaction with polyelectrolyte. By tuning the unit molar ratio of the opposite charged polyelectrolytes and the amount of Fe3O4 nanoparticles, the structure of the composite microspheres could be controlled and the dimension of microspheres ranged from 110 nm to 320 nm. Then the approach was extended to prepare luminescent-magnetic CS–PAsp microspheres by encapsulated Fe3O4 nanoparticles and CdTe quantum dots synchronously, which exhibited superior pH stability and remained strong photoluminescence properties after crosslinking. The CdTe–Fe3O4/CS–PAsp composite microspheres are potentially useful for further application in biolabeling and imaging.Functional inorganic/organic composite microspheres were prepared by encapsulating inorganic nanoparticles (Fe3O4, CdTe QDs) into CS–PAsp microspheres via electrostatic interaction between the polyelectrolytes and inorganic nanoparticles.
Co-reporter:Cheng Chi, Yi Shi, Hong Zheng, Yahong Zhang, Wei Chen, Wuli Yang, Yi Tang
Materials Chemistry and Physics 2009 Volume 115(2–3) pp:808-814
Publication Date(Web):15 June 2009
DOI:10.1016/j.matchemphys.2009.02.026
Organic species are thought to exert an important effect on the formation of the mineral materials by their electrostatic attraction with the cations of minerals. In this work, a series of polyaspartic acids with different hydroxylation degrees (PAsp-x%OH) have been used as crystal growth modifiers to direct the synthesis of calcium phosphate. The change of x in the PAsp-x%OH can precisely adjust its electrostatic interaction with calcium ions and, thereby, modulate the formation process and property of calcium phosphate, such as morphology, crystallinity, organic component content and calcium-to-phosphate ratio. Two competitive reactions are suggested in this system, that is, the combination of calcium ions to PAsp-x%OH and the precipitation of calcium hydrogen phosphate dihydrate. The trends of these two reactions are determined by variation of x in the PAsp-x%OH: lower value of x tends to involve the former, while the higher tends to latter. It has been found that the mineralization process involving PAsp-15%OH displayed a special point to counterbalance the two competitive reactions, leading to the longest induction time. These findings indicate that how an organic species controls the morphology and the formation dynamics of inorganic crystals in biomineralization by the slight modification of its molecular structure.
Co-reporter:Jie Cai;Jia Guo;Minglei Ji;Changchun Wang
Colloid and Polymer Science 2007 Volume 285( Issue 14) pp:1607-1615
Publication Date(Web):2007 November
DOI:10.1007/s00396-007-1735-4
Fe3O4/SiO2/poly (N-isopropylacrylamide-co-N,N-dimethylaminoethyl methacrylate) [P(NIPAM-co-DMA)] multiresponsive composite microspheres with core–shell structure were synthesized by template precipitation polymerization. First, the magnetite nanoparticles were coated with silica and then modified with 3-(trimethoxysilyl)-propyl methacrylate (MPS). Subsequently, the Fe3O4/SiO2 particles grafted with MPS were used to seed the precipitation copolymerization of NIPAM and DMA. The composite microspheres with core–shell structure were superparamagnetic, pH-sensitive, and thermoresponsive. The swelling ratio (D25 °C,pH = 3/D50 °C,pH = 9)3 coupling of pH and temperature increased up to 21.2, which was much higher than that without comonomer DMA.
Co-reporter:Xiaoqing Ren, Rui Zheng, Xiaoling Fang, Xiaofei Wang, Xiaoyan Zhang, Wuli Yang, Xianyi Sha
Biomaterials (June 2016) Volume 92() pp:13-24
Publication Date(Web):June 2016
DOI:10.1016/j.biomaterials.2016.03.026
Along with intrinsic magnetic resonance imaging (MRI) advantages, iron oxide nanomaterials capable of photothermal conversion have been reported very recently and have again raised great interest in their designs among biomedical researchers. However, like other inorganic nanomaterials, high macrophage uptake, short blood retention time and unfavorable biodistributions have strongly hampered their applications in vivo. To solve these problems, a rational design of red blood cell (RBC) membrane camouflaged iron oxide magnetic clusters (MNC@RBCs) is presented in this paper. Our data show that by simply introducing an “ultra-stealth” biomimetic coating to iron oxide magnetic nanoclusters (MNCs), MNC@RBCs maintain the imaging and photothermal functionalities inherited from MNCs cores while achieving much lower nonspecific macrophage uptake and dramatically altered fate in vivo. MNC@RBCs with superior prolonged blood retention time, preferred high tumor accumulation and relatively lowered liver biodistribution are demonstrated when injected intravenously in mice, leading to greatly enhanced photothermal therapeutic efficacy by a single treatment without further magnetic force manipulation. Our study illustrates a well prepared integration of MNCs and RBCs, exploiting advantages of both functionalities within a single unit and suggests a promising future for iron-based nanomaterials application in vivo.
Co-reporter:Xiaoqing Ren, Rui Zheng, Xiaoling Fang, Xiaofei Wang, Xiaoyan Zhang, Wuli Yang, Xianyi Sha
Biomaterials (June 2016) Volume 92() pp:13-24
Publication Date(Web):June 2016
DOI:10.1016/j.biomaterials.2016.03.026
Co-reporter:Yi Shi, Yahong Zhang, Wuli Yang and Yi Tang
Chemical Communications 2009(Issue 4) pp:NaN444-444
Publication Date(Web):2008/11/19
DOI:10.1039/B814095B
A series of multiradiate calcium phosphate patterns have been observed by a gradating chitosan-polyaspartatic acid (PAsp) system, and their morphology evolution reveals the effect of chitosan and PAsp on the interfacial biomineralization.
Co-reporter:Congying Liu, Jia Guo, Wuli Yang, Jianhua Hu, Changchun Wang and Shoukuan Fu
Journal of Materials Chemistry A 2009 - vol. 19(Issue 27) pp:NaN4770-4770
Publication Date(Web):2009/05/27
DOI:10.1039/B902985K
A controlled drug release system was designed based on the combination of three advantages into one entity, which was composed of Fe3O4 magnetic nanoparticle as the core, mesoporous silica as the sandwiched layer, and thermo-sensitive P(NIPAM-co-NHMA) copolymer as the outer shell. The hydrophilic comonomer content affected the volume phase-transition temperature (VPTT) of this composite microsphere and the behavior of the temperature-triggered drug release. Zn(II) phthalocyanine tetrasulfonic acid (ZnPcS4), a well-known photodynamic therapy (PDT) drug, was used as a model drug to assess the release system. The results demonstrated that the drug release behavior was dependent on the temperature and had a close correlation with the VPTT. Above the VPTT, the drug release rate was much faster than that below the VPTT, which showed a great potential application in tumor therapy.
Co-reporter:Baisong Chang, Jia Guo, Congying Liu, Ji Qian and Wuli Yang
Journal of Materials Chemistry A 2010 - vol. 20(Issue 44) pp:NaN9947-9947
Publication Date(Web):2010/09/24
DOI:10.1039/C0JM01237H
The modified sol–gel approach to the synthesis of well-structured magnetic mesoporous silica nanoparticles (M-MSNs) was described, which comprised magnetic nanoparticles resided within the mesoporous nanoparticles. A diversity of surface modification was subjected to systematic investigation using organic silanes, eventually resulting in the decoration with the carboxyl (–COOH), methyl phosphonate (–PO3−), amino (–NH2) and phenyl (–Ph) groups on the surface of M-MSNs. The careful characterizations demonstrated that the modified M-MSNs displayed the specifically charged surfaces and differently porous characters, yet without showing any influence on the shape and size. To exploit their potential in cancer treatment, we extensively studied the drug loading capacity and sustained release behaviour of the modified M-MSNs for representative drugs. The hydrophilically modified M-MSNs with –COOH and –PO3− were beneficial for loading the water-soluble doxorubicin hydrochloride (DOX) through electrostatic attraction. The results demonstrated that M-MSNs-PO3− achieved a higher loading content and M-MSNs-COOH presented a distinct pH-responsible release behavior. On the other hand, M-MSNs-Ph displayed a controlled release rate in a short term via the weakened hydrogen bonding interaction. The cytotoxicity of modified M-MSNs to normal cells and macrophage uptake indicated that the modified M-MSNs were suitable as drug carriers. These mesoporous nanoparticles were non-toxicity to HeLa cells, while the drug-loaded nanoparticles apparently led to the unambiguous cytotoxicity as a result of the sustained release of drugs. These results have an important implication that the modified M-MSNs are promising as platforms for storing the hydrophilic or hydrophobic anticancer drugs for tumour therapy.
Co-reporter:Yunfeng Jiao, Jia Guo, Shun Shen, Baisong Chang, Yahong Zhang, Xinguo Jiang and Wuli Yang
Journal of Materials Chemistry A 2012 - vol. 22(Issue 34) pp:NaN17643-17643
Publication Date(Web):2012/07/10
DOI:10.1039/C2JM31821K
By employing poly (tert-butylacrylate) (PTBA) nanospheres as the dissolvable core templates, we develop a new method to synthesize hollow mesoporous silica nanoparticles (HMSN). Both the PTBA core and the structure-directing surfactant, cetyltrimethylammonium bromide (CTAB), can be easily and synchronously removed through solvent extraction in ethanol, which ensures the complete structure and ideal dispersibility of the products compared with the previous template synthesis that often needs calcination to remove the templates. Given that hollow core diameter and shell thickness are the key properties of HMSN, the hollow core diameter and shell thickness can be tailored precisely. In addition, as novel inorganic nanomaterials with a tuned structure, HMSN show notable biocompatibility and efficient doxorubicin (DOX) loading. In in vitro tests, the release rate of DOX-loaded HMSN exhibit a surprising shell-thickness-dependent and a pH responsive drug release character, suggesting that HMSN are a very promising drug delivery system for shell-thickness-controlled drug release. The results of intracellular tracking and cytotoxicity assays further demonstrate the potential and efficiency of HMSN as a drug delivery system.
Co-reporter:Hongyan Tang, Shun Shen, Jia Guo, Baisong Chang, Xinguo Jiang and Wuli Yang
Journal of Materials Chemistry A 2012 - vol. 22(Issue 31) pp:NaN16103-16103
Publication Date(Web):2012/06/13
DOI:10.1039/C2JM32599C
Novel composite nanoparticles based on poly(N-isopropylacrylamide-co-N-hydroxymethyl acrylamide) (P(NIPAM-co-NHMA)) layer coated gold nanorod@mesoporous silica (GNR@mSiO2) has been successfully synthesized by precipitation polymerization. The composite nanoparticles exhibited a thermo/near-infrared (NIR) light sensitivity. The volume phase transition temperature (VPTT) could be precisely regulated by the content of NHMA in monomers and an excellent photothermal conversion effect was expressed due to the surface plasmon resonance of gold nanorods in the composite nanoparticles. Doxorubicin hydrochloride (DOX) was applied as a model drug and the drug storage/release behavior was investigated. The results demonstrated that DOX could be effectively loaded into the composite nanoparticles with 21% loading capacity. The cumulative release of DOX-loaded composite nanoparticles was temperature dependent and the release rate was significantly enhanced above the VPTT. Therefore, the composite nanoparticles applied as a drug delivery system could reduce the side effect of DOX to normal tissues as only a small fraction of DOX was released from the composite nanoparticles at 37 °C. In addition, the DOX-loaded composite nanoparticles demonstrated a synergistic effect, the therapeutic efficacy was improved significantly by the combination of photothermal therapy and traditional chemotherapy with low composite nanoparticle concentration and short laser irradiation time in an in vitro study.
Co-reporter:Yang Wang, Jin Zheng, Yefei Tian and Wuli Yang
Journal of Materials Chemistry A 2015 - vol. 3(Issue 28) pp:NaN5832-5832
Publication Date(Web):2015/06/16
DOI:10.1039/C5TB00703H
To improve the biocompatibility and biodegradability of nanocarriers, well-defined poly(vinylcaprolactam)-based acid degradable nanogels were fabricated for drug delivery via precipitation polymerization in water, where synthetic ketal-based 2,2-dimethacroyloxy-1-ethoxypropane (DMAEP) acted as a cross-linker, and N-(2-hydroxypropyl)methacrylamide (HPMA) served as a co-monomer. Expectedly, we observed that the temperature and pH of the environment play important roles in the performance of the nanogels. The nanogels were reduced in size upon increasing the temperature and showed higher volume phase transition temperature (VPTT) with higher concentration of HPMA. With the incorporation of ketal linkages, the nanogels showed accelerated degradation profiles by lowering the pH and increasing temperature of the incubation medium. When used as nanocarriers of anticancer drug doxorubicin (DOX), compared to non-degradable nanogels with similar components, the acid-degradable nanogels displayed more effective drug controlled release behaviour, low drug leakage of DOX at neutral pH while rapid and sufficient release from the nanogels under acidic conditions. The results of the cytotoxicity and hemolysis assays further highlighted that the acid-degradable nanogel produced no hemolysin but showed excellent viability to normal cells, and the DOX-loaded nanogel exhibited higher proliferation inhibition against tumor cells.
Co-reporter:Baisong Chang, Xianyi Sha, Jia Guo, Yunfeng Jiao, Changchun Wang and Wuli Yang
Journal of Materials Chemistry A 2011 - vol. 21(Issue 25) pp:NaN9247-9247
Publication Date(Web):2011/05/23
DOI:10.1039/C1JM10631G
In this paper, a kind of core–shell composite microsphere was prepared based on poly(N-isopropylacrylamide-co-methacrylic acid) (P(NIPAM-co-MAA)) coated magnetic mesoporous silica nanoparticles (M-MSN) via precipitation polymerization. The composite microsphere presented a thermo/pH-coupling sensitivity and the volume phase transition could be precisely regulated by the molar ratio of MAA to NIPAM or the concentration of NaCl. At physiological conditions (37 °C, 0.15 M NaCl), the P(NIPAM-co-MAA) shell underwent a distinct transition from a swollen state in pH 7.4 to a collapsed state in pH 5.0, so that the polymer shell was active in moderating the diffusion of embedded drugs in-and-out of the pore channels of MSN. Doxorubicin hydrochloride (DOX) was applied as a model drug and the behaviors of drug storage/release were investigated. The drug loaded behavior was pH-dependent, and the composite microsphere had a drug embed efficiency of about 91.3% under alkaline conditions. The cumulative in vitro release of the DOX-loaded composite microsphere showed a low level of leakage below the volume phase transition temperature (VPTT) and was significantly enhanced above its VPTT, exhibiting an apparent thermo/pH-response controlled drug release. The cytotoxicity assay of a blank carrier to normal cells indicated that the composite microspheres were suitable as drug carriers, while the DOX-loaded composite microspheres had a similar cytotoxicity to HeLa cells compared with free DOX. Therefore, the thermo/pH-sensitive composite microsphere could, in principle, be used for in vivo cancer therapy with a low premature drug release during blood circulation whilst having a rapid release upon reaching tumor tissues.
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