Jiang-Li Fan

In Situ Colorimetric Recognition of Melamine Based on Thymine Derivative-Functionalized Gold Nanoparticle

Co-reporter:Jianjun Du;Xiaojun Peng;Zhenkuan Wang;Jiangli Fan

Industrial & Engineering Chemistry Research December 9, 2015 Volume 54(Issue 48) pp:12011-12016

Publication Date(Web):2017-2-22

DOI:10.1021/acs.iecr.5b02399

A simple, fast, and convenient colorimetric sensing system is presented for melamine recognition in milk samples by thymine-derivative- (NT-) decorated gold nanoparticles (AuNPs), based on the complementary hydrogen bond between thymine and melamine, wherein the introduction of melamine causes a rapid and obvious red-to-blue color change. Therefore, the melamine content in milk can be qualitatively recognized by the naked eye and quantitatively measured by measuring absorbance. NT-AuNPs show excellent selectivity to melamine against any other tested molecules, anions, and metal ions, as well as very good sensitivity that can distinguish melamine-contaminated milk from safe milk with a limit of detection of 3.5 nM.

A dynamic invertible intramolecular charge-transfer fluorescence probe: real-time monitoring of mitochondrial ATPase activity

Co-reporter:Hua Zhang;Yafu Wang;Xiaopeng Xuan;Ge Wang;Haiming Guo;Jiangli Fan

Chemical Communications 2017 vol. 53(Issue 40) pp:5535-5538

Publication Date(Web):2017/05/17

DOI:10.1039/C7CC02450A

A dynamic invertible intramolecular charge-transfer (ICT) process could provide abundant response signals for real-time monitoring in living organisms. Herein, based on dynamic invertible ICT, we have reported a cancer cell-targeted fluorescence probe (OPM) for mitochondrial ATPase activity. Due to its abundant response signals, OPM could real-time monitor mitochondrial ATPase activity during the cancer apoptosis process, successfully.

Lighting-up breast cancer cells by a near-infrared fluorescent probe based on KIAA1363 enzyme-targeting

Co-reporter:Jiangli Fan;Shigang Guo;Suzhen Wang;Yao Kang;Qichao Yao;Jia Wang;Xue Gao;Hongjiang Wang;Jianjun Du;Xiaojun Peng

Chemical Communications 2017 vol. 53(Issue 35) pp:4857-4860

Publication Date(Web):2017/04/27

DOI:10.1039/C7CC01798G

The first NIR KIAA1363-targeting probe, NB-AX, specifically and instantly featured an “off–on” switch upon gradual addition of KIAA1363 over all kinds of other biomolecules, and its detection limit was initially calculated to be 0.58 μg mL−1 (3δ/k). The probe was also able to be used in ultrafast distinguishing of breast cancer cells from normal cells in fluorescence imaging and applied in tissue imaging and tumor imaging in vivo.

A α-KA fluorescent probe for discrimination of blood cancer serum

Co-reporter:Yao Kang, Jiangli Fan, Qiang Jin, Chenhui Shi, ... Xiaojun Peng

Chinese Chemical Letters 2017 Volume 28, Issue 10(Volume 28, Issue 10) pp:

Publication Date(Web):1 October 2017

DOI:10.1016/j.cclet.2017.08.054

α-Ketoglutaric acid (α-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body. The mutations of isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2), however, will lead to the transformation of α-KA into 2-hydroxyglutarate (2-HG), which is confirmed to closely related to actue myeloid leukemia (AML). Therefore it is of great significance to detect α-KA level changes in serum. In this paper, a fluorescent “off-on” probe CH for α-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for α-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide (CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement ((I − I0)/I0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with α-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing α-KA and may promote fluorescence detection in clinical diagnosis.Download high-res image (116KB)Download full-size imageProbe CH can quickly detect α-KA in human serum with micelles of CTAB and could differentiate cancer from normal blood serum.

Fluorescent, MRI, and colorimetric chemical sensors for the first-row d-block metal ions

Co-reporter:Hao Zhu, Jiangli Fan, Benhua Wang and Xiaojun Peng

Chemical Society Reviews 2015 vol. 44(Issue 13) pp:4337-4366

Publication Date(Web):12 Nov 2014

DOI:10.1039/C4CS00285G

Transition metals (d-blocks) are recognized as playing critical roles in biology, and they most often act as cofactors in diverse enzymes; however, improper regulation of transition metal stores is also connected to serious disorders. Therefore, the monitoring and imaging of transition metals are significant for biological research as well as clinical diagnosis. In this article, efforts have been made to review the chemical sensors that have been developed for the detection of the first-row d-block metals (except Cu and Zn): Cr, Mn, Fe, Co, and Ni. We focus on the development of fluorescent sensors (fall into three classes: “turn-off”, “turn-on”, and ratiometric), colorimetric sensors, and responsive MRI contrast agents for these transition metals (242 references). Future work will be likely to fill in the blanks: (1) sensors for Sc, Ti, and V; (2) MRI sensors for Cr, Mn, Co, Ni; (3) ratiometric fluorescent sensors for Cr6+, Mn2+, and Ni2+, explore new ways of sensing Fe3+ or Cr3+ without the proton interference, as well as extend applications of MRI sensors to living systems.

A Nile blue based infrared fluorescent probe: imaging tumors that over-express cyclooxygenase-2

Co-reporter:Benhua Wang, Jiangli Fan, Xianwu Wang, Hao Zhu, Jingyun Wang, Huiying Mu and Xiaojun Peng

Chemical Communications 2015 vol. 51(Issue 4) pp:792-795

Publication Date(Web):19 Nov 2014

DOI:10.1039/C4CC08915D

The first Golgi-localized cyclooxygenase-2 (COX-2)-specific near-infrared (NIR) fluorescent probe, Niblue-C6-IMC, able to detect cancer cells, was designed. Importantly, Niblue-C6-IMC preferentially labeled the tumors in a mouse tumor model with deep tissue penetration capacity. It may be a promising molecular tool for guiding tumor resection during surgery.

Light up ClO− in live cells using an aza-coumarin based fluorescent probe with fast response and high sensitivity

Co-reporter:Jiangli Fan, Huiying Mu, Hao Zhu, Jingyun Wang and Xiaojun Peng

Analyst 2015 vol. 140(Issue 13) pp:4594-4598

Publication Date(Web):08 May 2015

DOI:10.1039/C5AN00777A

Hypochlorous acid (HClO)/hypochlorite (ClO−), one of the reactive oxygen species (ROS), is a key microbicidal agent used for natural defense; however, HClO is also responsible for some human diseases. Although much effort has been made to develop HClO-selective fluorescent probes, many of them display a delayed response time and nanomole-sensitive probes are rare. In this study, we designed and synthesized an aza-coumarin based fluorescent probe AC-ClO for ClO− determination with fast response (completed within 2 min) and high sensitivity (detection limit is 25 nM). AC-ClO displayed a color change from pink to light yellow and a remarkable “turn-on” fluorescence response towards ClO−. Confocal fluorescence microscopy experiments demonstrated that the probe could be applied for the live-cell imaging of exogenous and endogenous ClO−.

Recognition of HClO in Live Cells with Separate Signals Using a Ratiometric Fluorescent Sensor with Fast Response

Co-reporter:Jiangli Fan, Huiying Mu, Hao Zhu, Jianjun Du, Na Jiang, Jingyun Wang, and Xiaojun Peng

Industrial & Engineering Chemistry Research 2015 Volume 54(Issue 36) pp:8842-8846

Publication Date(Web):August 26, 2015

DOI:10.1021/acs.iecr.5b01904

Hypochlorous acid (HClO), one of the reactive oxygen species (ROS), is a key microbicidal agent which is used for natural defense; however, it is also involved in several human diseases. Although several efforts have been made to develop HClO-selective fluorescent sensors, most of them display delayed response time and the ratiometric sensors are rare. In the current work, we used a 3-pyrrole BODIPY dye as a new ratiometric fluorescent sensor (BRClO) for HClO. BRClO exhibited a 177-fold fluorescence ratio (F505 nm/F585 nm) enhancement in the presence of HClO over other ROS with fast response time (completed within 1 s). Confocal fluorescence imaging with BRClO showed separated signals in the presence of exogenous HClO in live cells. In addition, BRClO could be easily prepared as HClO test strips to detect HClO in aqueous solution and natural tap water.

Ratiometric Fluorescence Imaging of Cellular Polarity: Decrease in Mitochondrial Polarity in Cancer Cells

Co-reporter:Na Jiang;Jiangli Fan;Feng Xu; Xiaojun Peng;Huiying Mu; Jingyun Wang;Xiaoqing Xiong

Angewandte Chemie International Edition 2015 Volume 54( Issue 8) pp:2510-2514

Publication Date(Web):

DOI:10.1002/anie.201410645

Abstract

Mitochondrial polarity strongly influences the intracellular transportation of proteins and interactions between biomacromolecules. The first fluorescent probe capable of the ratiometric imaging of mitochondrial polarity is reported. The probe, termed BOB, has two absorption maxima (λ_abs=426 and 561 nm) and two emission maxima—a strong green emission (λ_em=467 nm) and a weak red emission (642 nm in methanol)—when excited at 405 nm. However, only the green emission is markedly sensitive to polarity changes, thus providing a ratiometric fluorescence response with a good linear relationship in both extensive and narrow ranges of solution polarity. BOB possesses high specificity to mitochondria (R_r=0.96) that is independent of the mitochondrial membrane potential. The mitochondrial polarity in cancer cells was found to be lower than that of normal cells by ratiometric fluorescence imaging with BOB. The difference in mitochondrial polarity might be used to distinguish cancer cells from normal cells.

Ratiometric Fluorescence Imaging of Cellular Polarity: Decrease in Mitochondrial Polarity in Cancer Cells

Co-reporter:Na Jiang;Jiangli Fan;Feng Xu; Xiaojun Peng;Huiying Mu; Jingyun Wang;Xiaoqing Xiong

Angewandte Chemie 2015 Volume 127( Issue 8) pp:2540-2544

Publication Date(Web):

DOI:10.1002/ange.201410645

Abstract

Mitochondrial polarity strongly influences the intracellular transportation of proteins and interactions between biomacromolecules. The first fluorescent probe capable of the ratiometric imaging of mitochondrial polarity is reported. The probe, termed BOB, has two absorption maxima (λ_abs=426 and 561 nm) and two emission maxima—a strong green emission (λ_em=467 nm) and a weak red emission (642 nm in methanol)—when excited at 405 nm. However, only the green emission is markedly sensitive to polarity changes, thus providing a ratiometric fluorescence response with a good linear relationship in both extensive and narrow ranges of solution polarity. BOB possesses high specificity to mitochondria (R_r=0.96) that is independent of the mitochondrial membrane potential. The mitochondrial polarity in cancer cells was found to be lower than that of normal cells by ratiometric fluorescence imaging with BOB. The difference in mitochondrial polarity might be used to distinguish cancer cells from normal cells.

An “Enhanced PET”-Based Fluorescent Probe with Ultrasensitivity for Imaging Basal and Elesclomol-Induced HClO in Cancer Cells

Co-reporter:Hao Zhu ; Jiangli Fan ; Jingyun Wang ; Huiying Mu ;Xiaojun Peng

Journal of the American Chemical Society 2014 Volume 136(Issue 37) pp:12820-12823

Publication Date(Web):August 29, 2014

DOI:10.1021/ja505988g

Reactive oxygen species (ROS) and cellular oxidant stress have long been associated with cancer. Unfortunately, the role of HClO in tumor biology is much less clear than for other ROS. Herein, we report a BODIPY-based HClO probe (BClO) with ultrasensitivity, fast response (within 1 s), and high selectivity, in which the pyrrole group at the meso position has an “enhanced PET” effect on the BODIPY fluorophore. The detection limit is as low as 0.56 nM, which is the highest sensitivity achieved to date. BClO can be facilely synthesized by a Michael addition reaction of acryloyl chloride with 2,4-dimethylpyrrole and applied to image the basal HClO in cancer cells for the first time and the time-dependent HClO generation in MCF-7 cells stimulated by elesclomol, an effective experimental ROS-generating anticancer agent.

A bright red fluorescent cyanine dye for live-cell nucleic acid imaging, with high photostability and a large Stokes shift

Co-reporter:Si Zhang, Jiangli Fan, Zhiyong Li, Naijia Hao, Jianfang Cao, Tong Wu, Jingyun Wang and Xiaojun Peng

Journal of Materials Chemistry A 2014 vol. 2(Issue 18) pp:2688-2693

Publication Date(Web):24 Jan 2014

DOI:10.1039/C3TB21844A

Many probes for nucleic acids are available, but few of them satisfy multiple criteria, particularly high photostability to endure laser scanning. We report a cyanine dye TO3-CN for the first time, synthesised by introducing a CN group to the trimethine chain of the classical red emitting TO-3 dye to improve its photostability, as well as their spectral properties and interaction with nucleic acids. TO3-CN shows excellent light fastness and a large fluorescence Stokes shift (more than 40 nm). Because of its sensitive fluorescence response to nucleic acids with a large fluorescence quantum yield (more than 0.7) and low cytotoxicity, this dye may be a potential candidate for nucleic acid detection in vitro and intracellular fluorescence imaging.

A fluorescent probe for site I binding and sensitive discrimination of HSA from BSA

Co-reporter:Jiangli Fan, Wen Sun, Zhenkuan Wang, Xiaojun Peng, Yueqing Li and Jianfang Cao

Chemical Communications 2014 vol. 50(Issue 67) pp:9573-9576

Publication Date(Web):04 Jul 2014

DOI:10.1039/C4CC03778B

A fluorescent probe DH1 has been successfully developed to detect HSA via site I non-covalent bonding. DH1 shows a dramatic fluorescence enhancement towards HSA without interference from other proteins. The molecular docking method, for the first time, was utilized to provide deep insight into the sensing mechanism of the probe. Moreover, probe DH1 was successfully used to detect trace HSA in healthy human urine.

A near-infrared fluorescent probe for selective detection of HClO based on Se-sensitized aggregation of heptamethine cyanine dye

Co-reporter:Guanghui Cheng, Jiangli Fan, Wen Sun, Jianfang Cao, Chong Hu and Xiaojun Peng

Chemical Communications 2014 vol. 50(Issue 8) pp:1018-1020

Publication Date(Web):14 Nov 2013

DOI:10.1039/C3CC47864E

A Se-containing heptamethine cyanine dye based fluorescent probe was successfully developed and used for HClO detection with rapid response and high selectivity based on aggregation behavior. The probe could react with HClO with significant change in its fluorescence profile, which makes it practical for detecting HClO in fetal bovine serum and in living mice.

Fluorescence imaging lysosomal changes during cell division and apoptosis observed using Nile Blue based near-infrared emission

Co-reporter:Jiangli Fan, Huijuan Dong, Mingming Hu, Jingyun Wang, Hua Zhang, Hao Zhu, Wen Sun and Xiaojun Peng

Chemical Communications 2014 vol. 50(Issue 7) pp:882-884

Publication Date(Web):12 Nov 2013

DOI:10.1039/C3CC48043G

NBM, a Nile Blue based NIR fluorescent dye was reported. NBM is highly effective in specifically labeling lysosomes with low concentration and good photostability in various kinds of live cells. It was first successfully used to observe the disappearance and reproduction of lysosomes during cell division.

Ratiometric fluorescence imaging of lysosomal Zn2+ release under oxidative stress in neural stem cells

Co-reporter:Hao Zhu, Jiangli Fan, Shiling Zhang, Jianfang Cao, Kedong Song, Dan Ge, Huijuan Dong, Jingyun Wang and Xiaojun Peng

Biomaterials Science 2014 vol. 2(Issue 1) pp:89-97

Publication Date(Web):11 Sep 2013

DOI:10.1039/C3BM60186B

Zinc dyshomeostasis is a major mechanism of neuronal death, which is involved in many different neuropathological conditions. Lysosomal membrane permeabilisation has an important function in zinc-induced neuronal death under oxidative stress. To investigate lysosomal zinc functions in neurons with high spatial and temporal reliability, we report a ratiometric probe, LysoZn-1. It is derived from the styryl-BODIPY-DPA scaffold with a lysosome-targeted 2-morpholinoethylamine moiety to allow localisation in lysosomes. The electron donor at the meso-position of the BODIPY fluorophore makes the present probe prefer complexing with Zn2+ rather than Cd2+, which can be explained by HSAB (Hard–Soft Acid–Base) theory and was confirmed by Gaussian calculation. Upon Zn2+ binding, LysoZn-1 exhibits obvious fluorescence enhancement (F578nm) and ratio (F578nm/F680nm) changes. The emission intensities of LysoZn-1 and LysoZn-1 + Zn2+ do not change significantly under lysosomal pH ranging from 4.5 to 6.0. Confocal imaging experiments indicate that LysoZn-1 is able to localise to lysosomes in neural stem cells (NSCs), MCF-7 and Hela cells and detect exogenous Zn2+ levels in NSCs and MCF-7 cells. LysoZn-1 function is not disturbed by chloroquine in living cells. Furthermore, increases in lysosomal Zn2+ concentration upon H2O2 stimulation in NSCs are observed using LysoZn-1.

Dual mode monitoring probe for mitochondrial viscosity in single cell

Co-reporter:Na Jiang, Jiangli Fan, Si Zhang, Tong Wu, Jingyun Wang, Pan Gao, Junle Qu, Fan Zhou, Xiaojun Peng

Sensors and Actuators B: Chemical 2014 190() pp: 685-693

Publication Date(Web):

DOI:10.1016/j.snb.2013.09.062

A Distorted-BODIPY-Based Fluorescent Probe for Imaging of Cellular Viscosity in Live Cells

Co-reporter:Hao Zhu;Dr. Jiangli Fan;Miao Li;Jianfang Cao;Dr. Jingyun Wang;Dr. Xiaojun Peng

Chemistry - A European Journal 2014 Volume 20( Issue 16) pp:4691-4696

Publication Date(Web):

DOI:10.1002/chem.201304296

Abstract

Cellular viscosity is a critical factor in governing diffusion-mediated cellular processes and is linked to a number of diseases and pathologies. Fluorescent molecular rotors (FMRs) have recently been developed to determine viscosity in solutions or biological fluid. Herein, we report a “distorted-BODIPY”-based probe BV-1 for cellular viscosity, which is different from the conventional “pure rotors”. In BV-1, the internal steric hindrance between the meso-CHO group and the 1,7-dimethyl group forced the boron–dipyrrin framework to be distorted, which mainly caused nonradiative deactivation in low-viscosity environment. BV-1 gave high sensitivity (x=0.62) together with stringent selectivity to viscosity, thus enabling viscosity mapping in live cells. Significantly, the increase of cytoplasmic viscosity during apoptosis was observed by BV-1 in real time.

An Off–On COX-2-Specific Fluorescent Probe: Targeting the Golgi Apparatus of Cancer Cells

Co-reporter:Hua Zhang, Jiangli Fan, Jingyun Wang, Shuangzhe Zhang, Bairui Dou, and Xiaojun Peng

Journal of the American Chemical Society 2013 Volume 135(Issue 31) pp:11663-11669

Publication Date(Web):July 17, 2013

DOI:10.1021/ja4056905

Identifying cancer cells and quantifying cancer-related events in particular organelles in a rapid and sensitive fashion are important for early diagnosis and for studies on pathology and therapeutics of cancers. Herein a smart “off–on” cyclooxygenase-2-specific fluorescence probe (ANQ-IMC-6), able to report the presence of cancer cells and to image Golgi-related events, has been designed and evaluated. Cyclooxygenase-2 (COX-2) has been used as imaging target in the probe design, since this enzyme is a biomarker of virtually all cancer cell lines. In the free state in aqueous solution, ANQ-IMC-6 mainly exists in a folded conformation where probe fluorescence is quenched through photoinduced electron transfer between the fluorophore acenaphtho[1,2-b]quinoxaline (ANQ) and the recognition group, indomethacin (IMC). Fluorescence is turned on, by restraining the photoinduced electron transfer, when ANQ-IMC-6 is forced to adopt the unfolded state following binding to COX-2 in the Golgi apparatus of cancer cells. ANQ-IMC-6 provides high signal-to-background staining and has been successfully used to rapidly differentiate cancer cells from normal cells when using flow cytometry and one- and two-photon fluorescence microscopic imaging. Furthermore, ANQ-IMC-6 may be able to visualize dynamic changes of the Golgi apparatus during cancer cell apoptosis, with possible application to early diagnosis.

Fluorescence Discrimination of Cancer from Inflammation by Molecular Response to COX-2 Enzymes

Co-reporter:Hua Zhang ; Jiangli Fan ; Jingyun Wang ; Bairui Dou ; Fan Zhou ; Jianfang Cao ; Junle Qu ; Zhi Cao ; Weijie Zhao ;Xiaojun Peng

Journal of the American Chemical Society 2013 Volume 135(Issue 46) pp:17469-17475

Publication Date(Web):October 22, 2013

DOI:10.1021/ja4085308

Accurate identification of cancer from inflammation and normal tissue in a rapid, sensitive, and quantitative fashion is important for cancer diagnosis and resection during surgery. Here we report the use of cyclooxygenase-2 as a marker for identification of cancer from inflammation and the design of a novel smart COX-2-specific fluorogenic probe (NANQ-IMC6). The probe’s fluorescence is “turned on” in both inflammations and cancers where COX-2 is overexpressed. Intriguingly, the fluorescent emission is quite different at these two sites with different expression level of COX-2. Hence, NANQ-IMC6 can not only distinguish normal cells/tissues from cancer cells/tissues but also distinguish the latter from sites of inflammation lesions by the different fluorescence recognition of NANQ-IMC6 for COX-2 enzymes. Following spraying with the NANQ-IMC6 solution, cancerous tissue, inflamed tissues, and normal tissues can be accurately discriminated in vivo by the unaided eye using a hand-held ultraviolet lamp emitting at 365 nm. So the probe may have potential application varying from cancer inflammation diagnosis to guiding tumor resection during surgery.

Fluorene-derived two-photon fluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and lysosomes: group-effect and localization

Co-reporter:Hua Zhang, Jiangli Fan, Huijuan Dong, Shuangzhe Zhang, Wenyou Xu, Jingyun Wang, Pan Gao and Xiaojun Peng

Journal of Materials Chemistry A 2013 vol. 1(Issue 40) pp:5450-5455

Publication Date(Web):08 Aug 2013

DOI:10.1039/C3TB20646G

Two novel fluorene-derived two-photon fluorescent probes (TPFL) targeting the endoplasmic reticulum (TPFL-ER) and lysosomes (TPFL-Lyso) were synthesized by introducing a chlorine group and a morpholine group, respectively. They were shown to be suitable for specific and simultaneous imaging of the endoplasmic reticulum and lysosomes without affecting protein movements owing to their similar molecular structures. TPFL-ER and TPFL-Lyso were successfully used to visualize the changes of the endoplasmic reticulum and lysosomes during cancer cell apoptosis and they demonstrated high specificity and sensitivity, excellent photostability, and low phototoxicity.

A Fluorescent Ratiometric Chemodosimeter for Cu2+ Based on TBET and Its Application in Living Cells

Co-reporter:Jiangli Fan, Peng Zhan, Mingming Hu, Wen Sun, Jizhou Tang, Jingyun Wang, Shiguo Sun, Fengling Song, and Xiaojun Peng

Organic Letters 2013 Volume 15(Issue 3) pp:492-495

Publication Date(Web):January 22, 2013

DOI:10.1021/ol3032889

Based on a through bond energy transfer (TBET) between Rhodamine and a naphthalimide fluorophore, a fluorescent ratiometric chemodosimeter RN1 was designed and prepared for single selective detection of Cu2+ in aqueous solution and in living cells, as Cu2+ acts as not only a selective recognizing guest but also a hydrolytic promoter.

A near-infrared dye based on BODIPY for tracking morphology changes in mitochondria

Co-reporter:Na Jiang, Jiangli Fan, Tao Liu, Jianfang Cao, Bo Qiao, Jingyun Wang, Pan Gao and Xiaojun Peng

Chemical Communications 2013 vol. 49(Issue 90) pp:10620-10622

Publication Date(Web):24 Sep 2013

DOI:10.1039/C3CC46143B

M-DPT, a BODIPY-based water soluble near-infrared fluorescent probe with thiophene at the 1,7-position, is synthesized. M-DPT is found to possess high specificity to mitochondria, superior photostability, and appreciable tolerance to microenvironmental changes. Thus, this probe is a highly suitable imaging agent for targeting mitochondria and tracking morphology changes.

A two-photon fluorescent probe with near-infrared emission for hydrogen sulfide imaging in biosystems

Co-reporter:Wen Sun, Jiangli Fan, Chong Hu, Jianfang Cao, Hua Zhang, Xiaoqing Xiong, Jingyun Wang, Shuang Cui, Shiguo Sun and Xiaojun Peng

Chemical Communications 2013 vol. 49(Issue 37) pp:3890-3892

Publication Date(Web):21 Mar 2013

DOI:10.1039/C3CC41244J

Hydrogen sulfide (H2S) is emerging as an important gasotransmitter but remains difficult to study. Here we report a novel two-photon fluorescent probe with NIR emission for H2S detection. It was successfully used to realize H2S imaging in bovine serum, living cells, tissues as well as in living mice.

A highly specific BODIPY-based probe localized in mitochondria for HClO imaging

Co-reporter:Guanghui Cheng, Jiangli Fan, Wen Sun, Kun Sui, Xin Jin, Jingyun Wang and Xiaojun Peng

Analyst 2013 vol. 138(Issue 20) pp:6091-6096

Publication Date(Web):25 Jul 2013

DOI:10.1039/C3AN01152F

An oxime containing fluorescent probe based on a BODIPY scaffold was successfully designed and used for HClO determination with rapid response, low detection limits and high selectivity. Confocal fluorescence microscopy demonstrated that the probe could permeate the mitochondria and made possible the fast fluorescent imaging of endogenous HClO.

Rhodamine-based ‘turn-on’ fluorescent probe for Cu(II) and its fluorescence imaging in living cells

Co-reporter:Mao-Zhong Tian, Ming-Ming Hu, Jiang-Li Fan, Xiao-Jun Peng, Jing-Yun Wang, Shi-Guo Sun, Rong Zhang

Bioorganic & Medicinal Chemistry Letters 2013 Volume 23(Issue 10) pp:2916-2919

Publication Date(Web):15 May 2013

DOI:10.1016/j.bmcl.2013.03.052

A novel rhodamine spirolactam derivative 3′,6′-Bis(diethylamino)-2-(2-hydroxyethylamino) spiro[isoindoline-1,9′-xanthen]-3-one (RO1) was synthesized, and characterized by high-resolution mass spectrometry (HRMS), X-ray crystallography, Infrared spectroscopy (IR), and 1H NMR and 13C NMR spectroscopy. RO1 exhibited highly sensitive and exclusively selective fluorescence response toward Cu2+ over other metal ions with a detection limit of 0.56 ppb in mixed aqueous solution. The fluorescence was pH-independent in the wide range pH 3.1–11.6. The turn-on fluorescence enhancement of the probe is based on Cu2+ induced ring-opening mechanism of the rhodamine spirolactam. Moreover, by means of fluorescence microscopy experiments, it was demonstrated that RO1 could monitor trace Cu2+ changes by live cell imaging.RO1 exhibited highly sensitive and exclusively selective fluorescence response toward Cu2+ over other metal ions with a detection limit of 0.56 ppb in mixed aqueous solution. The fluorescence was pH-independent in the wide range pH 3.1–11.6. probe RO1 can penetrate the cell membrane and be applied for in vitro imaging of Cu2+ in living cells.

Optical Pd2+ sensing by rhodamine hydrazone ligands: different stoichiometries in aqueous/nonaqueous environments

Co-reporter:Honglin Li, Jianfang Cao, Hao Zhu, Jiangli Fan, Xiaojun Peng

Tetrahedron Letters 2013 Volume 54(Issue 33) pp:4357-4361

Publication Date(Web):14 August 2013

DOI:10.1016/j.tetlet.2013.05.094

A series of rhodamine spiro-leuco compounds have been developed as fluorescence-enhanced sensors for optical sensing of palladium ion (Pd2+). The initial rhodamine hydrazone derivatives, RPd1-a, RPd1-b, and RPd1-c, display a good coordination to Pd2+ inducing the spirolactam-open reaction with a significant fluorescence enhancement and color generation. Different stoichiometry results, 1:2 in EtOH/H2O and 1:1 in MeOH/DCM were observed for Pd2+/RPd1-c complexation.

Fluorescent chemodosimeters using “mild” chemical events for the detection of small anions and cations in biological and environmental media

Co-reporter:Jianjun Du, Mingming Hu, Jiangli Fan and Xiaojun Peng

Chemical Society Reviews 2012 vol. 41(Issue 12) pp:4511-4535

Publication Date(Web):25 Apr 2012

DOI:10.1039/C2CS00004K

Mild chemical processes of various analytes and detection methods involving revolutionary strategies in the fields of analytical chemistry, biology and environmental sciences have been extensively developed. This critical review focuses on representative examples of mild chemical processes that can be used in fluorescent chemodosimeters for ion sensing (anions and cations). A systematisation according to the type of reaction mechanism is established. Numerous examples including extensions combined with catalytic and material sciences applicable in fluorescence imaging and water treatment are also discussed (151 references).

Imaging of lysosomal pH changes with a fluorescent sensor containing a novel lysosome-locating group

Co-reporter:Hao Zhu, Jiangli Fan, Qunli Xu, Honglin Li, Jingyun Wang, Pan Gao and Xiaojun Peng

Chemical Communications 2012 vol. 48(Issue 96) pp:11766-11768

Publication Date(Web):23 Oct 2012

DOI:10.1039/C2CC36785H

Rlyso, a highly selective and sensitive pH sensor, can stain lysosomes with a novel lysosome-locating group, methylcarbitol. Rlyso was successfully used to detect lysosomal pH changes during apoptosis or induced by chloroquine while avoiding the “alkalizing effect” on lysosomes of current lysosomal probes with nitrogen-containing sidechains.

An ICT-based ratiometric probe for hydrazine and its application in live cells

Co-reporter:Jiangli Fan, Wen Sun, Mingming Hu, Jianfang Cao, Guanghui Cheng, Huijuan Dong, Kedong Song, Yingchao Liu, Shiguo Sun and Xiaojun Peng

Chemical Communications 2012 vol. 48(Issue 65) pp:8117-8119

Publication Date(Web):21 Jun 2012

DOI:10.1039/C2CC34168A

Hydrazine is an important industrial chemical but also very toxic thus requiring rapid detection agents. A ratiometric fluorescence probe that enables rapid, low-limit and naked-eye detection is successfully designed and used for hydrazine determination in live cells.

Development of an oxidative dehydrogenation-based fluorescent probe for Cu2+ and its biological imaging in living cells

Co-reporter:Jiangli Fan, Xiaojian Liu, Mingming Hu, Hao Zhu, Fengling Song, Xiaojun Peng

Analytica Chimica Acta 2012 Volume 735() pp:107-113

Publication Date(Web):20 July 2012

DOI:10.1016/j.aca.2012.05.027

Based on a boron dipyrromethene (BODIPY) derivative containing an N, O and S tridentate ligand, a Cu2+ fluorescent probe BTCu was developed. The detection mechanism was verified as Cu2+-promoted oxidative dehydrogenation of an amine moiety, leading to a formation of a fluorescent Cu+–Schiff base complex. Free BTCu exhibited a maximum absorption wavelength at 496 nm, and a very weak maximum emission at 511 nm. Upon addition of various metals ions, it showed large fluorescence enhancement toward Cu2+ (417-fold in MeCN and 103-fold in MeCN/HEPES solution, respectively) with high selectivity. The detection limits are as low as 1.74 × 10−8 M and 4.96 × 10−8 M in the two different solutions, respectively. And BTCu could work in a wide pH range with an extraordinary low pKa of 1.21 ± 0.06. Using fluorescence microscopy, the probe was shown to be capable of penetrating into living cells and imaging intracellular Cu2+ changes.Graphical abstractHighlights► The fluorescent probe contains an N, O and S tridentate ligand. ► The probe is simple but highly sensitive and selective toward Cu2+. ► The mechanism is based on the Cu2+-promoted dehydrogenation of amine in different organic and aqueous solutions. ► It was successfully applied to visualize Cu2+ in living cells.

A fluorescent sensor for pH based on rhodamine fluorophore

Co-reporter:Maozhong Tian, Xiaojun Peng, Jiangli Fan, Jingyun Wang, Shiguo Sun

Dyes and Pigments 2012 Volume 95(Issue 1) pp:112-115

Publication Date(Web):October 2012

DOI:10.1016/j.dyepig.2012.03.008

In this study, an ‘Off–On’-type acidic pH fluorescent chemosensor, 3′,6′-Bis (ethylamino)-2′,7′-dimethyl-2-(2-oxoethylideneamino)spiro[isoindoline-1,9′-xanthen]-3-one (RG1) has been designed, synthesized, and characterized by high-resolution mass spectrometry (HRMS), X-ray crystallography, infrared spectroscopy (IR), and 1H NMR and 13C NMR spectroscopy. The design tactics for the sensor was based on the change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine dyes. Fluorescence “off–on” behaviors of RG1 were investigated on the basis of variable acid concentrations. The pH titrations showed a ca. 581-fold increase in fluorescence intensity within the pH range of 7.5 to 1.1 with a pKa value of 2.32 in acetonitrile–water (1:1, v/v) solution when excited at 510 nm. The fluorescence change of RG1 was fully reversible and took place mainly within the pH range from 1.0 to 4.0, which was valuable for pH researches in acidic environments.Highlights► A pH fluorescent chemosensor RG1 based on rhodamine dye was synthesized. ► RG1 showed a 581-fold fluorescence enhancement in aqueous solution. ► The fluorescence change of RG1 is fully reversible. ► RG1 is valuable for pH researches in acidic environments with a pKa of 2.32.

Enhanced fluorescent chemosensor for Ag+ in absolute aqueous solution and living cells: An experimental and theoretical study

Co-reporter:Mingming Hu, Jiangli Fan, Jianfang Cao, Kedong Song, Hua Zhang, Shiguo Sun and Xiaojun Peng

Analyst 2012 vol. 137(Issue 9) pp:2107-2111

Publication Date(Web):24 Feb 2012

DOI:10.1039/C2AN16272E

Rosamine derivative RC-1, bearing macrocyclic ligand [15]aneNO2S2 as receptor, was synthesized as an enhanced fluorescent chemosensor for Ag+ in absolute aqueous solution. The fluorescence images in living cells show the potential application of RC-1. The difference of fluorescence enhancement process after binding with Ag+/Hg2+ in acetonitrile/water solution was first proved by theoretical calculations.

Optical Cu2+ probe bearing an 8-hydroxyquinoline subunit: High sensitivity and large fluorescence enhancement

Co-reporter:Hao Zhu, Jiangli Fan, Jing Lu, Mingming Hu, Jianfang Cao, Jing Wang, Honglin Li, Xiaojian Liu, Xiaojun Peng

Talanta 2012 Volume 93() pp:55-61

Publication Date(Web):15 May 2012

DOI:10.1016/j.talanta.2012.01.024

A new fluorescent and colorimetric probe RCu1 with ultra-sensitivity (detection limit is 4.7 nM (3σ)) was developed. It is based on a rhodamine B derivative modified with a functionalized 8-hydroxyquinoline group as a copper-binding site which greatly increases the affinity for Cu2+. Cu2+ acts not only as a selective recognizing guest but also a hydrolytic promoter. RCu1 showed single-selectively sensing Cu2+ over other cations and much larger fluorescence enhancement (as high as over 1000-fold) than those based on Cu2+-complexation. The detection mechanism was proved by TOF-MS, FT-IR, 1H NMR and Gaussian calculations. Based on the outstanding recognition ability of RCu1 toward Cu2+, an analytical procedure was developed for Cu2+-determination in natural water samples and soil sample.Graphical abstractHighlights► A new fluorescent and colorimetric probe RCu1 with ultra-sensitivity (detection limit is 4.7 nM (3σ)) was developed. ► RCu1 showed greatly large fluorescence enhancement (F/F0 > 1000) in the presence of Cu2+ in aqueous media. ► The detection mechanism was proved by TOF-MS, FT-IR, 1H NMR and Gaussian calculations. ► Based on the outstanding recognition ability of RCu1 toward Cu2+, an analytical procedure was developed for Cu2+-determination in natural water samples and soil sample.

A Fluorescence Turn-on Sensor for Hg2+ with a Simple Receptor Available in Sulphide-Rich Environments

Co-reporter:Jiangli Fan;Xiaojun Peng;Song Wang;Xiaojian Liu;Honglin Li

Journal of Fluorescence 2012 Volume 22( Issue 3) pp:945-951

Publication Date(Web):2012 May

DOI:10.1007/s10895-011-1033-x

Detection of Hg2+ in complex natural environmental conditions is extremely challenging, and no entirely successful methods currently exist. Here we report an easy-to-prepare fluorescent sensor B3 with 2-aminophenol as Hg2+ receptor, which exhibits selective fluorescence enhancement toward Hg2+ over other metal ions. Especially, the fluorescence enhancement was unaffected by anions and cations existing in environment and organism. Moreover, B3 can detect Hg2+ in sulphide-rich environments without cysteine, S2- or EDTA altering the fluorescence intensity. Consequently, B3 is capable of distinguishing between safe and toxic levels of Hg2+ in more complicated natural water systems with detection limit ≤2 ppb.

Highly Sensitive and Fast-Responsive Fluorescent Chemosensor for Palladium: Reversible Sensing and Visible Recovery

Co-reporter:Dr. Honglin Li;Dr. Jiangli Fan;Mingming Hu;Guanghui Cheng; Danhong Zhou;Tong Wu;Dr. Fengling Song;Dr. Shiguo Sun; Chunying Duan; Xiaojun Peng

Chemistry - A European Journal 2012 Volume 18( Issue 39) pp:12242-12250

Publication Date(Web):

DOI:10.1002/chem.201201998

Abstract

The well-known rhodamine spiro-lactam framework offers an ideal model for the development of fluorescence-enhanced chemosensors through simple and convenient syntheses. Herein, we report a new tridentate PNO receptor, which was introduced into a rhodamine spiro-lactam system to develop Pd²⁺-chemosensor RPd4, that displayed significantly improved sensing properties for palladium. Compound RPd4 shows a very fast response time (about 5 s), high sensitivity (5 nM), and excellent specificity for Pd²⁺ ions over other PGE ions (Pt²⁺, Rh³⁺, and Ru³⁺). In addition, RPd4 displays quite different responses to different valence states of the Pd ions, that is, very fast response towards Pd²⁺ ions but slow response towards Pd⁰, which may provide us with a convenient method for the selective discrimination of Pd species in different valence states. According to proof-of-concept experiments, RPd4 has potential applications in Pd²⁺-analysis in drug compounds, water, soil, and leaf samples. Owing to its good reversibility, RPd4 can also be used as a sensor material for the selective detection and visual recovery of trace Pd²⁺ ions in environmental samples.

Fluorescent chemodosimeter for Cys/Hcy with a large absorption shift and imaging in living cells

Co-reporter:Mingming Hu, Jiangli Fan, Honglin Li, Kedong Song, Song Wang, Guanghui Cheng and Xiaojun Peng

Organic & Biomolecular Chemistry 2011 vol. 9(Issue 4) pp:980-983

Publication Date(Web):16 Dec 2010

DOI:10.1039/C0OB00957A

A novel molecule T1 with efficient intramolecular charge transfer was designed as a fluorescent chemodosimeter for cysteine (Cys) and homocysteine (Hcy). Upon addition of Cys/Hcy, T1 exhibited greatly enhanced fluorescence intensity as well as a large absorption peak shift (70 nm), and can be used for bioimaging of Cys/Hcy in living cells and detection in human plasma by visual color change. The detection mechanism was proved by 1H NMR, mass spectrometry analysis and Gaussian calculations.

Fluorescence completely separated ratiometric probe for HClO in lysosomes

Co-reporter:Zhen Zhang, Jiangli Fan, Guanghui Cheng, Sahar Ghazali, Jianjun Du, Xiaojun Peng

Sensors and Actuators B: Chemical (July 2017) Volume 246() pp:

Publication Date(Web):July 2017

DOI:10.1016/j.snb.2017.02.081

•A ratiometric probe (BFClO) with completely seperated fluorescent signals for sensing HOCl is reported.•BFClO exhibits superior selectivity (>300 fold) and fast response (within 10 s) for HClO over other ROS in a broad pH range.•BFClO can be used for visualizing exogenous and endogenous HOCl in lysosomes of living cells.Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), was reported to be involved in a number of human diseases and many efforts have therefore been made to probe its biological roles at molecular level. In this work, a ratiometric probe (BFClO) with completely separated fluorescent signals consisting of a boron-dipyrromethene (BODIPY) dye conjugated with Fisher aldehyde was designed and synthesized for the selective and sensitive detection of HOCl via oxidative cleavage of the alkene linker. The probe evinced more than 300-fold enhancement in fluorescence ratio (I511nm/I713nm) in the presence of HClO over other ROS with fast response time (within 10 s) and low detection limit (10.6 nM). Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane and visualize exogenous and endogenous HOCl in lysosomes of living cells.

A bright red fluorescent cyanine dye for live-cell nucleic acid imaging, with high photostability and a large Stokes shift

Co-reporter:Si Zhang, Jiangli Fan, Zhiyong Li, Naijia Hao, Jianfang Cao, Tong Wu, Jingyun Wang and Xiaojun Peng

Journal of Materials Chemistry A 2014 - vol. 2(Issue 18) pp:NaN2693-2693

Publication Date(Web):2014/01/24

DOI:10.1039/C3TB21844A

Many probes for nucleic acids are available, but few of them satisfy multiple criteria, particularly high photostability to endure laser scanning. We report a cyanine dye TO3-CN for the first time, synthesised by introducing a CN group to the trimethine chain of the classical red emitting TO-3 dye to improve its photostability, as well as their spectral properties and interaction with nucleic acids. TO3-CN shows excellent light fastness and a large fluorescence Stokes shift (more than 40 nm). Because of its sensitive fluorescence response to nucleic acids with a large fluorescence quantum yield (more than 0.7) and low cytotoxicity, this dye may be a potential candidate for nucleic acid detection in vitro and intracellular fluorescence imaging.

Lighting-up breast cancer cells by a near-infrared fluorescent probe based on KIAA1363 enzyme-targeting

Co-reporter:Jiangli Fan, Shigang Guo, Suzhen Wang, Yao Kang, Qichao Yao, Jia Wang, Xue Gao, Hongjiang Wang, Jianjun Du and Xiaojun Peng

Chemical Communications 2017 - vol. 53(Issue 35) pp:NaN4860-4860

Publication Date(Web):2017/04/05

DOI:10.1039/C7CC01798G

The first NIR KIAA1363-targeting probe, NB-AX, specifically and instantly featured an “off–on” switch upon gradual addition of KIAA1363 over all kinds of other biomolecules, and its detection limit was initially calculated to be 0.58 μg mL−1 (3δ/k). The probe was also able to be used in ultrafast distinguishing of breast cancer cells from normal cells in fluorescence imaging and applied in tissue imaging and tumor imaging in vivo.

A dynamic invertible intramolecular charge-transfer fluorescence probe: real-time monitoring of mitochondrial ATPase activity

Co-reporter:Hua Zhang, Yafu Wang, Xiaopeng Xuan, Ge Wang, Haiming Guo and Jiangli Fan

Chemical Communications 2017 - vol. 53(Issue 40) pp:NaN5538-5538

Publication Date(Web):2017/04/24

DOI:10.1039/C7CC02450A

A dynamic invertible intramolecular charge-transfer (ICT) process could provide abundant response signals for real-time monitoring in living organisms. Herein, based on dynamic invertible ICT, we have reported a cancer cell-targeted fluorescence probe (OPM) for mitochondrial ATPase activity. Due to its abundant response signals, OPM could real-time monitor mitochondrial ATPase activity during the cancer apoptosis process, successfully.

Ratiometric fluorescence imaging of lysosomal Zn2+ release under oxidative stress in neural stem cells

Co-reporter:Hao Zhu, Jiangli Fan, Shiling Zhang, Jianfang Cao, Kedong Song, Dan Ge, Huijuan Dong, Jingyun Wang and Xiaojun Peng

Biomaterials Science (2013-Present) 2014 - vol. 2(Issue 1) pp:NaN97-97

Publication Date(Web):2013/09/11

DOI:10.1039/C3BM60186B

Zinc dyshomeostasis is a major mechanism of neuronal death, which is involved in many different neuropathological conditions. Lysosomal membrane permeabilisation has an important function in zinc-induced neuronal death under oxidative stress. To investigate lysosomal zinc functions in neurons with high spatial and temporal reliability, we report a ratiometric probe, LysoZn-1. It is derived from the styryl-BODIPY-DPA scaffold with a lysosome-targeted 2-morpholinoethylamine moiety to allow localisation in lysosomes. The electron donor at the meso-position of the BODIPY fluorophore makes the present probe prefer complexing with Zn2+ rather than Cd2+, which can be explained by HSAB (Hard–Soft Acid–Base) theory and was confirmed by Gaussian calculation. Upon Zn2+ binding, LysoZn-1 exhibits obvious fluorescence enhancement (F578nm) and ratio (F578nm/F680nm) changes. The emission intensities of LysoZn-1 and LysoZn-1 + Zn2+ do not change significantly under lysosomal pH ranging from 4.5 to 6.0. Confocal imaging experiments indicate that LysoZn-1 is able to localise to lysosomes in neural stem cells (NSCs), MCF-7 and Hela cells and detect exogenous Zn2+ levels in NSCs and MCF-7 cells. LysoZn-1 function is not disturbed by chloroquine in living cells. Furthermore, increases in lysosomal Zn2+ concentration upon H2O2 stimulation in NSCs are observed using LysoZn-1.

A near-infrared fluorescent probe for selective detection of HClO based on Se-sensitized aggregation of heptamethine cyanine dye

Co-reporter:Guanghui Cheng, Jiangli Fan, Wen Sun, Jianfang Cao, Chong Hu and Xiaojun Peng

Chemical Communications 2014 - vol. 50(Issue 8) pp:NaN1020-1020

Publication Date(Web):2013/11/14

DOI:10.1039/C3CC47864E

A Se-containing heptamethine cyanine dye based fluorescent probe was successfully developed and used for HClO detection with rapid response and high selectivity based on aggregation behavior. The probe could react with HClO with significant change in its fluorescence profile, which makes it practical for detecting HClO in fetal bovine serum and in living mice.

A fluorescent probe for site I binding and sensitive discrimination of HSA from BSA

Co-reporter:Jiangli Fan, Wen Sun, Zhenkuan Wang, Xiaojun Peng, Yueqing Li and Jianfang Cao

Chemical Communications 2014 - vol. 50(Issue 67) pp:NaN9576-9576

Publication Date(Web):2014/07/04

DOI:10.1039/C4CC03778B

A fluorescent probe DH1 has been successfully developed to detect HSA via site I non-covalent bonding. DH1 shows a dramatic fluorescence enhancement towards HSA without interference from other proteins. The molecular docking method, for the first time, was utilized to provide deep insight into the sensing mechanism of the probe. Moreover, probe DH1 was successfully used to detect trace HSA in healthy human urine.

A Nile blue based infrared fluorescent probe: imaging tumors that over-express cyclooxygenase-2

Co-reporter:Benhua Wang, Jiangli Fan, Xianwu Wang, Hao Zhu, Jingyun Wang, Huiying Mu and Xiaojun Peng

Chemical Communications 2015 - vol. 51(Issue 4) pp:NaN795-795

Publication Date(Web):2014/11/19

DOI:10.1039/C4CC08915D

The first Golgi-localized cyclooxygenase-2 (COX-2)-specific near-infrared (NIR) fluorescent probe, Niblue-C6-IMC, able to detect cancer cells, was designed. Importantly, Niblue-C6-IMC preferentially labeled the tumors in a mouse tumor model with deep tissue penetration capacity. It may be a promising molecular tool for guiding tumor resection during surgery.

Fluorescence imaging lysosomal changes during cell division and apoptosis observed using Nile Blue based near-infrared emission

Co-reporter:Jiangli Fan, Huijuan Dong, Mingming Hu, Jingyun Wang, Hua Zhang, Hao Zhu, Wen Sun and Xiaojun Peng

Chemical Communications 2014 - vol. 50(Issue 7) pp:NaN884-884

Publication Date(Web):2013/11/12

DOI:10.1039/C3CC48043G

NBM, a Nile Blue based NIR fluorescent dye was reported. NBM is highly effective in specifically labeling lysosomes with low concentration and good photostability in various kinds of live cells. It was first successfully used to observe the disappearance and reproduction of lysosomes during cell division.

A two-photon fluorescent probe with near-infrared emission for hydrogen sulfide imaging in biosystems

Co-reporter:Wen Sun, Jiangli Fan, Chong Hu, Jianfang Cao, Hua Zhang, Xiaoqing Xiong, Jingyun Wang, Shuang Cui, Shiguo Sun and Xiaojun Peng

Chemical Communications 2013 - vol. 49(Issue 37) pp:NaN3892-3892

Publication Date(Web):2013/03/21

DOI:10.1039/C3CC41244J

Hydrogen sulfide (H2S) is emerging as an important gasotransmitter but remains difficult to study. Here we report a novel two-photon fluorescent probe with NIR emission for H2S detection. It was successfully used to realize H2S imaging in bovine serum, living cells, tissues as well as in living mice.

An ICT-based ratiometric probe for hydrazine and its application in live cells

Co-reporter:Jiangli Fan, Wen Sun, Mingming Hu, Jianfang Cao, Guanghui Cheng, Huijuan Dong, Kedong Song, Yingchao Liu, Shiguo Sun and Xiaojun Peng

Chemical Communications 2012 - vol. 48(Issue 65) pp:NaN8119-8119

Publication Date(Web):2012/06/21

DOI:10.1039/C2CC34168A

Hydrazine is an important industrial chemical but also very toxic thus requiring rapid detection agents. A ratiometric fluorescence probe that enables rapid, low-limit and naked-eye detection is successfully designed and used for hydrazine determination in live cells.

Fluorescent, MRI, and colorimetric chemical sensors for the first-row d-block metal ions

Co-reporter:Hao Zhu, Jiangli Fan, Benhua Wang and Xiaojun Peng

Chemical Society Reviews 2015 - vol. 44(Issue 13) pp:NaN4366-4366

Publication Date(Web):2014/11/12

DOI:10.1039/C4CS00285G

Transition metals (d-blocks) are recognized as playing critical roles in biology, and they most often act as cofactors in diverse enzymes; however, improper regulation of transition metal stores is also connected to serious disorders. Therefore, the monitoring and imaging of transition metals are significant for biological research as well as clinical diagnosis. In this article, efforts have been made to review the chemical sensors that have been developed for the detection of the first-row d-block metals (except Cu and Zn): Cr, Mn, Fe, Co, and Ni. We focus on the development of fluorescent sensors (fall into three classes: “turn-off”, “turn-on”, and ratiometric), colorimetric sensors, and responsive MRI contrast agents for these transition metals (242 references). Future work will be likely to fill in the blanks: (1) sensors for Sc, Ti, and V; (2) MRI sensors for Cr, Mn, Co, Ni; (3) ratiometric fluorescent sensors for Cr6+, Mn2+, and Ni2+, explore new ways of sensing Fe3+ or Cr3+ without the proton interference, as well as extend applications of MRI sensors to living systems.

Fluorene-derived two-photon fluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and lysosomes: group-effect and localization

Co-reporter:Hua Zhang, Jiangli Fan, Huijuan Dong, Shuangzhe Zhang, Wenyou Xu, Jingyun Wang, Pan Gao and Xiaojun Peng

Journal of Materials Chemistry A 2013 - vol. 1(Issue 40) pp:NaN5455-5455

Publication Date(Web):2013/08/08

DOI:10.1039/C3TB20646G

Two novel fluorene-derived two-photon fluorescent probes (TPFL) targeting the endoplasmic reticulum (TPFL-ER) and lysosomes (TPFL-Lyso) were synthesized by introducing a chlorine group and a morpholine group, respectively. They were shown to be suitable for specific and simultaneous imaging of the endoplasmic reticulum and lysosomes without affecting protein movements owing to their similar molecular structures. TPFL-ER and TPFL-Lyso were successfully used to visualize the changes of the endoplasmic reticulum and lysosomes during cancer cell apoptosis and they demonstrated high specificity and sensitivity, excellent photostability, and low phototoxicity.

Fluorescent chemodosimeter for Cys/Hcy with a large absorption shift and imaging in living cells

Co-reporter:Mingming Hu, Jiangli Fan, Honglin Li, Kedong Song, Song Wang, Guanghui Cheng and Xiaojun Peng

Organic & Biomolecular Chemistry 2011 - vol. 9(Issue 4) pp:NaN983-983

Publication Date(Web):2010/12/16

DOI:10.1039/C0OB00957A

A novel molecule T1 with efficient intramolecular charge transfer was designed as a fluorescent chemodosimeter for cysteine (Cys) and homocysteine (Hcy). Upon addition of Cys/Hcy, T1 exhibited greatly enhanced fluorescence intensity as well as a large absorption peak shift (70 nm), and can be used for bioimaging of Cys/Hcy in living cells and detection in human plasma by visual color change. The detection mechanism was proved by 1H NMR, mass spectrometry analysis and Gaussian calculations.

Fluorescent chemodosimeters using “mild” chemical events for the detection of small anions and cations in biological and environmental media

Co-reporter:Jianjun Du, Mingming Hu, Jiangli Fan and Xiaojun Peng

Chemical Society Reviews 2012 - vol. 41(Issue 12) pp:NaN4535-4535

Publication Date(Web):2012/04/25

DOI:10.1039/C2CS00004K

Mild chemical processes of various analytes and detection methods involving revolutionary strategies in the fields of analytical chemistry, biology and environmental sciences have been extensively developed. This critical review focuses on representative examples of mild chemical processes that can be used in fluorescent chemodosimeters for ion sensing (anions and cations). A systematisation according to the type of reaction mechanism is established. Numerous examples including extensions combined with catalytic and material sciences applicable in fluorescence imaging and water treatment are also discussed (151 references).

Imaging of lysosomal pH changes with a fluorescent sensor containing a novel lysosome-locating group

Co-reporter:Hao Zhu, Jiangli Fan, Qunli Xu, Honglin Li, Jingyun Wang, Pan Gao and Xiaojun Peng

Chemical Communications 2012 - vol. 48(Issue 96) pp:NaN11768-11768

Publication Date(Web):2012/10/23

DOI:10.1039/C2CC36785H

Rlyso, a highly selective and sensitive pH sensor, can stain lysosomes with a novel lysosome-locating group, methylcarbitol. Rlyso was successfully used to detect lysosomal pH changes during apoptosis or induced by chloroquine while avoiding the “alkalizing effect” on lysosomes of current lysosomal probes with nitrogen-containing sidechains.

A near-infrared dye based on BODIPY for tracking morphology changes in mitochondria

Co-reporter:Na Jiang, Jiangli Fan, Tao Liu, Jianfang Cao, Bo Qiao, Jingyun Wang, Pan Gao and Xiaojun Peng

Chemical Communications 2013 - vol. 49(Issue 90) pp:NaN10622-10622

Publication Date(Web):2013/09/24

DOI:10.1039/C3CC46143B

M-DPT, a BODIPY-based water soluble near-infrared fluorescent probe with thiophene at the 1,7-position, is synthesized. M-DPT is found to possess high specificity to mitochondria, superior photostability, and appreciable tolerance to microenvironmental changes. Thus, this probe is a highly suitable imaging agent for targeting mitochondria and tracking morphology changes.

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