Co-reporter:Shuai Liu, Yongsheng Gao, Sigen A, Dezhong Zhou, Udo Greiser, Tianying Guo, Rui Guo, and Wenxin Wang
ACS Biomaterials Science & Engineering July 10, 2017 Volume 3(Issue 7) pp:1283-1283
Publication Date(Web):October 26, 2016
DOI:10.1021/acsbiomaterials.6b00503
To enhance the gene transfection efficiency to targeted cells while reducing the side effects to untargeted cells is of great significance for clinical gene therapy. Here, biodegradable highly branched poly(β-amino ester)s (HPAESS) are synthesized and functionalized with folate (HPAESS-FA) and lactobionic acid (HPAESS-Lac) for targeted cancer cell gene transfection. Results show that because of the triggered degradability of the vector and enhanced receptor-mediated cellular uptake of polyplexes, the HPAESS-FA and HPAESS-Lac exhibit superior gene transfection capability in specific cancer cells with negligible cytotoxicity, pointing to their promise as targeted vectors for efficient cancer gene therapy.Keywords: biodegradable; cancer cells; gene transfection; highly branched poly(β-amino ester)s; targeting;
Co-reporter:Shuai Liu, Dezhong Zhou, Jixiang Yang, Hao Zhou, Jiatong Chen, and Tianying Guo
Journal of the American Chemical Society April 12, 2017 Volume 139(Issue 14) pp:5102-5102
Publication Date(Web):March 21, 2017
DOI:10.1021/jacs.6b13337
To transform common low-molecular-weight (LMW) cationic polymers, such as polyethylenimine (PEI), to highly efficient gene vectors would be of great significance but remains challenging. Because LMW cationic polymers perform far less efficiently than their high-molecular-weight counterparts, mainly due to weaker nucleic acid encapsulation, herein we report the design and synthesis of a dipicolylamine-based disulfide-containing zinc(II) coordinative module (Zn-DDAC), which is used to functionalize LMW PEI (Mw ≈ 1800 Da) to give a non-viral vector (Zn-PD) with high efficiency and safety in primary and stem cells. Given its high phosphate binding affinity, Zn-DDAC can significantly promote the DNA packaging functionality of PEI1.8k and improve the cellular uptake of formulated polyplexes, which is particularly critical for hard-to-transfect cell types. Furthermore, Zn-PD polymer can be cleaved by glutathione in cytoplasm to facilitate DNA release post internalization and diminish the cytotoxicity. Consequently, the optimal Zn-PD mediates 1–2 orders of magnitude higher gluciferase activity than commercial transfection reagents, Xfect and PEI25k, across diverse cell types, including primary and stem cells. Our findings provide a valuable insight into the exploitation of LMW cationic polymers for gene delivery and demonstrate great promise for the development of next-generation non-viral vectors for clinically viable gene therapy.
Co-reporter:Shuai Liu;Zhibing Sun;Dezhong Zhou;Tianying Guo
Journal of Materials Chemistry B 2017 vol. 5(Issue 27) pp:5307-5310
Publication Date(Web):2017/07/12
DOI:10.1039/C7TB00996H
A branched poly(β-amino ester) with numerous alkyl chains (BPA) is designed and synthesized as a safe and efficient non-viral vector. The branching and hydrophobicity synergistically endow BPA with tight DNA condensation, high polyplex stability in serum, high cellular uptake and ultimately robust gene transfection efficiency, largely superior to its linear counterpart (LPA). Our results demonstrate that branching matters for gene delivery by hydrophobic gene vectors.
Co-reporter:Hongqi Ren, Shuai Liu, Jixiang Yang, Xian Zhang, Hao Zhou, Jiatong Chen, Tianying Guo
Carbohydrate Polymers 2016 Volume 137() pp:222-230
Publication Date(Web):10 February 2016
DOI:10.1016/j.carbpol.2015.10.085
•Present a new TMC chemical modification strategy by chitosan 6-hydroxy method.•Multi-functional oligomer assembly module prepared by RAFT polymerization method.•Building a TMC based high-performance gene carrier materials preparation platform.A novel non-viral gene carrier based on N,N,N-trimethylchitosan (TMC) has been fabricated. First, well-defined copolymer P(PEGMA-co-DMAEMA) was synthesized through reversible addition fragmentation chain transfer (RAFT) polymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and N,N-(2-dimethylamino)ethyl methacrylamide (DMAEMA). Then allyl group grafting N,N,N-trimethylchitosan (Allyl-TMC) was synthesized via the reaction between allyl bromide and hydroxyl of TMC. Finally, P(PEGMA-co-DMAEMA) and folate were ordinally grafted onto Allyl-TMC to obtain TMC-g-P(PEGMA-co-DMAEMA)-FA. In comparison with pristine chitosan, TMC-g-P(PEGMA-co-DMAEMA)-FA has achieved both better water solubility and stronger pDNA packaging ability, which can contribute to improving gene transfection. Gene delivery efficiency of a series of TMC based functional polymers with different chitosan molecular weights has been tested. The results show that 20k-TMC-g-P(PEGMA-co-DMAEMA)-FA/pDNA complex at the weight ratio of 20 achieve the highest transfection efficiency in 293 T cells. This work presents a new strategy to modify chitosan efficiently as gene carrier material.
Co-reporter:Shuai Liu, Jixiang Yang, Hongqi Ren, Jonathan O'Keeffe-Ahern, Dezhong Zhou, Hao Zhou, Jiatong Chen and Tianying Guo
Biomaterials Science 2016 vol. 4(Issue 3) pp:522-532
Publication Date(Web):21 Jan 2016
DOI:10.1039/C5BM00530B
Natural polycations, such as poly(L-lysine) (PLL) and chitosan (CS), have inherent superiority as non-viral vectors due to their unparalleled biocompatibility and biodegradability. However, the application was constrained by poor transfection efficiency and safety concerns. Since previous modification strategies greatly weakened the inherent advantages of natural polycations, developing a strategy for functional group introduction with broad applicability to enhance the transfection efficiency of natural polycations without compromising their cationic properties is imperative. Herein, two uncharged functional diblock oligomers P(DMAEL-b-NIPAM) and P(DMAEL-b-Vlm) were prepared from a lactose derivative, N-iso-propyl acrylamide (NIPAM) as well as 1-vinylimidazole (Vlm) and further functionalized with four small ligands folate, glutathione, cysteine and arginine, respectively, aiming to enhance the interactions of complexes with cells, which were quantified utilizing a quartz crystal microbalance (QCM) biosensor, circumventing the tedious material screening process of cell transfection. Upon incorporation with PLL and DNA, the multifunctional oligomers endow the formulated ternary complexes with great properties suitable for transfection, such as anti-aggregation in serum, destabilized endosome membrane, numerous functional sites for promoted endocytosis and therefore robust transfection activity. Furthermore, different from the conventional strategy of decreasing cytotoxicity by reducing the charge density, the multifunctional oligomer incorporation strategy maintains the highly positive charge density, which is essential for efficient cellular uptake. This system develops a new platform to modify natural polycations towards clinical gene therapy.
Co-reporter:Zhengsheng Liu;Shuai Liu;Heguang Shi;Hongqi Ren;Ruiyu Wang;Jixiang Yang ;Tianying Guo
Journal of Applied Polymer Science 2015 Volume 132( Issue 36) pp:
Publication Date(Web):
DOI:10.1002/app.42519
ABSTRACT
Thermoplastic polyurethane elastomers (TPU) were synthesized with isophorone diisocyanate (IPDI) as the rigid segment, poly(lactic-co-glycolic acid) (PLGA-PEG-PLGA) diol as soft segment, and 1,4-butanediol (BDO) as the chain extender. During the chain extension process, three kinds of fluorescent monomers 4-(2-hydroxyethylamino)−1,8-naphthoyl-(2-hydroxyethyl)imide (HNHI), 1,5-dihydroxy naphthalene (DHN), and dicoumarin (DIC) were introduced to get the fluorescently labeled degradable TPUs. The structure and degradation properties of the TPUs were characterized in detail. The results showed that there was no significant effect found on average molecular weight, mechanical properties, and glass transition temperature of polyurethane by introducing 0.001% (wt) weight percent of fluorescence monomers. The degradation behavior of fluorescent-tagged thermoplastic elastomer has been characterized with fluorescence microscopy. Results showed that polyurethane elastomers, in which fluorescence monomers especially HNHI were introduced by chemical reaction, had more homogeneous and stable fluorescence intensity than that with fluorescence monomers introduced by post blending. This work also provides a promising visual characterization approach to monitor degradation behavior of degradable TPUs for tissue engineering applications or drug delivery system. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42519.
Co-reporter:Wenhao Chi, Shuai Liu, Jixiang Yang, Ruiyu Wang, Hongqi Ren, Hao Zhou, Jiatong Chen and Tianying Guo
Journal of Materials Chemistry A 2014 vol. 2(Issue 33) pp:5387-5396
Publication Date(Web):12 Jun 2014
DOI:10.1039/C4TB00807C
Two kinds of novel oligomers were prepared by reversible addition fragmentation chain transfer (RAFT) polymerization and incorporated into the polyethyleneimine (PEI) gene delivery system through non-electrostatic assembly to improve gene transfection efficiency. The non-electrostatic assembly process was first investigated via probing the interactions of the oligomers with plasmid DNA (pDNA), PEI and AD-293 cells using a quartz crystal microbalance (QCM). The results show that the prepared oligomers almost had no interaction with pDNA while they had much stronger interactions with PEI and AD-293 cells. Meanwhile, we found that the two kinds of oligomers had different interactions with AD-193 cells, which caused different effects on gene transfection. The data of QCM tests combined with the in vitro transfection results can be used to explain what effects the oligomers have on improving gene transfection. The results also indicate that the strategy of detecting the interactions of oligomers with pDNA, polycations and cells will contribute to predetermine whether the prepared oligomer is efficient in improving gene transfection.
Co-reporter:Yong Guo, Ruiyu Wang, Wenhao Chi, Shuai Liu, Heguang Shi and Tianying Guo
RSC Advances 2014 vol. 4(Issue 16) pp:7881-7884
Publication Date(Web):04 Dec 2013
DOI:10.1039/C3RA45745A
A capsule with catalysis activity for pesticides degradation as well as adsorption activity for corresponding product elimination was developed by a simple one-step polymerization method based on molecular imprinting technology. More importantly, the absorption behavior of the capsule was investigated in detail by an in situ method, which showed that the capsule could absorb product more quickly.
Co-reporter:Lei Zhang;Yong Guo;Wen-hao Chi;He-guang Shi
Chinese Journal of Polymer Science 2014 Volume 32( Issue 11) pp:1469-1478
Publication Date(Web):2014 November
DOI:10.1007/s10118-014-1530-x
p-Nitrophenol imprinted nanoparticles with a size range of 150–300 nm in diameter were prepared through miniemulsion polymerization. The imprinted polymer exhibited higher adsorption capacity for p-nitrophenol than the nonimprinted polymer. The hydrolysis of paraoxon in aqueous phase can be accelerated in the presence of the p-nitrophenol imprinted nanoparticles. The hydrolysis rate of paraoxon incorporated with the imprinted nanoparticles was 2.83 × 10−7 mol/(L·min), which was about 3.7 times higher compared to the non-imprinted nanoparticles, 12.7 times higher to the spontaneous hydrolysis. The nanoparticles have been mixed with polyacrylonitrile solution and electrospun into nanofibers, which can also be used to accelerate the hydrolysis of paraoxon and conveniently separated from liquid phase for further processing.
Co-reporter:Yong Guo and Tianying Guo
Chemical Communications 2013 vol. 49(Issue 11) pp:1073-1075
Publication Date(Web):12 Dec 2012
DOI:10.1039/C2CC38022F
A new strategy of reactant–product-dual-template imprinting incorporated with hollow morphology to improve the catalysis efficiency of microspheres was developed. The synthesized capsule can achieve degradation of the reactant and elimination of its product simultaneously.
Co-reporter:Yuling Hu, Dezhong Zhou, Congxin Li, Hao Zhou, Jiatong Chen, Zhengpu Zhang, Tianying Guo
Acta Biomaterialia 2013 Volume 9(Issue 2) pp:5003-5012
Publication Date(Web):February 2013
DOI:10.1016/j.actbio.2012.09.033
Abstract
A novel functional diblock polymer P(PEGMA-b-MAH) is prepared and incorporated to improve the gene delivery efficiency of poly(ethyleneimine) PEI via non-covalent assembly strategy. First, P(PEGMA-b-MAH) is prepared from l-methacrylamidohistidine methyl ester (MAH) by reversible addition fragmentation chain transfer polymerization, with poly[poly(ethylene glycol) methyl ether methacrylate] (P(PEGMA)) as the macroinitiator. Then P(PEGMA-b-MAH) is assembled with plasmid DNA (pDNA) and PEI (Mw = 10 kDa) to form PEI/P(PEGMA-b-MAH)/pDNA ternary complexes. The agarose gel retardation assay shows that the presence of P(PEGMA-b-MAH) does not interfere with DNA condensation by the PEI. Dynamic light scattering tests show that PEI/P(PEGMA-b-MAH)/pDNA ternary complexes have excellent serum stability. In vitro transfection indicates that, compared to the P(PEGMA-b-MAH) free PEI-25k/pDNA binary complexes, PEI-10k/P(PEGMA-b-MAH)/pDNA ternary complexes have lower cytotoxicity and higher gene transfection efficiency, especially under serum conditions. The ternary complexes proposed here can inspire a new strategy for the development of gene and drug delivery vectors.
Co-reporter:Heguang Shi;Yue Li ;Tianying Guo
Journal of Applied Polymer Science 2013 Volume 128( Issue 5) pp:3163-3169
Publication Date(Web):
DOI:10.1002/app.38519
Abstract
Polyimide (PI) nanocomposites with 4,4′-bisphenol A dianhydride, 4,4′-oxydiphthalic anhydride, and diaminodiphenyl methane (MDA) as comonomers and functionalized with graphene oxide (GO), were prepared by in situ polymerization. Only a small amount of GO (0.03–0.12 wt %) is added to improve the mechanical properties of PI and to avoid a substantial decrease of PI transparence. The nanocomposites are characterized by FTIR, X-ray diffraction, thermogravimetric analysis, transmission electron microscope, tensile test, and UV-vis spectroscopy. It is demonstrated that the PI/GO composite films possess transmittance of above 80% at wavelengths of 500–800 nm when the GO content is under 0.12 wt %, while the stress intensity and Young's modulus are improved by 29 and 25%, respectively. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Co-reporter:Shuai Liu, Dezhong Zhou, Tianying Guo
Biosensors and Bioelectronics 2013 Volume 42() pp:80-86
Publication Date(Web):15 April 2013
DOI:10.1016/j.bios.2012.11.002
A novel quartz crystal microbalance (QCM) biosensor with high selectivity and sensitivity has been developed for ribonuclease A determination. Macroporous protein imprinted films have been fabricated on the surface of QCM electrode using 2,2,3,4,4,4-hexafluorobutyl methacrylate (HFBMA) as the main matrix monomer, N-methacryloyl-histidine (MAH) as the functional monomer, and trimethylolpropane trimethacrylate (TRIM) as the cross-linker. The imprinted special surface area and the quantity of the imprinted sites were increased by the formation of macropores that were generated by employing calcium carbonate nanoparticles as the porogen. The selectivity factor was improved obviously for the fluoromonomer containing system, especially in dilute protein solution, which gets benefit from the reducing of the nonspecific adsorption of proteins. Furthermore, MAH can not only play the role as the functional monomer, but also improve the hydrophilicity of surface of the imprinted film, which makes for the adsorption of proteins. At last, the rigid skeleton structure made the films durable in the recycledtests.Graphical abstractHighlights► We develop a QCM biosensor based on porous MIP film for ribonuclease A determination. ► We use fluoromonomer as the matrix, N-methacryloyl-histidine as functional monomer. ► The selectivity factor and response feature was improved obviously for the new system. ► The rigid skeleton structure made the films durable in the recycledtests.
Co-reporter:Dezhong Zhou, Congxin Li, Yuling Hu, Hao Zhou, Jiatong Chen, Zhengpu Zhang and Tianying Guo
Chemical Communications 2012 vol. 48(Issue 38) pp:4594-4596
Publication Date(Web):15 Mar 2012
DOI:10.1039/C2CC31359F
A novel multifunctional oligomer is synthesized and incorporated to enhance the gene delivery efficiency of PLL via non-electrostatic assembly and covalent grafting strategies. The improvement of the gene delivery efficiency is dependent on the gene carrying complex properties, and the properties are dependent on the oligomer incorporation strategy.
Co-reporter:Congxin Li, Dezhong Zhou, Yuling Hu, Hao Zhou, Jiatong Chen, Zhengpu Zhang, Tianying Guo
Carbohydrate Polymers 2012 Volume 89(Issue 1) pp:46-53
Publication Date(Web):5 June 2012
DOI:10.1016/j.carbpol.2012.02.035
The grafting modified chitosan with l-glutathione (GSH) end capped PEG brush-like poly [poly(ethylene glycol) methacrylate] (PMPEG), CS-PMPEG-GSH, as the pDNA condensed vector material could result in a much higher transfection efficiency and lower cytotoxity for NIH3T3 cells. In this work, we have further examined the morphology stabilities of CS-PMPEG-GSH/pDNA vectors at different medium pH values and in the presence of serum protein in detail. And then the targeted characters for HepG2 cells have been probed by tracing the cell uptake behavior and transfection efficiency.Highlights► Physiological stability of the glutathione and PEG modified chitosan as gene vector. ► Targeted characters to HepG2 cells probed by tracing cell uptake and transfection. ► The vector could result in much higher transfection efficiency and lower cytotoxity.
Co-reporter:Zhenhui Zhang, Yuling Hu, Zhengsheng Liu, Tianying Guo
Polymer 2012 Volume 53(Issue 14) pp:2979-2990
Publication Date(Web):21 June 2012
DOI:10.1016/j.polymer.2012.04.048
We report a homogeneous self-repairing copolymer system that exhibits self healing characteristics upon exposure to moisture environment. The copolymer consists of an isocyanate containing methacrylate monomer served as the repairing component and a fluorine-containing monomer as the protection component. Upon a mechanical damage of the coating copolymer, the isocyanate residue groups like the meshes of a zipper will exposure to generate reactive ends with environmental water like a faster of a zipper, thus form new crosslinking and repair the coating film. These materials with merits of easy-preparation and temperate repairing condition, can be used in decorative and anti-corrosion coatings.
Co-reporter:Kuichang Hua, Lei Zhang, Zhenhui Zhang, Yong Guo, Tianying Guo
Acta Biomaterialia 2011 Volume 7(Issue 8) pp:3086-3093
Publication Date(Web):August 2011
DOI:10.1016/j.actbio.2011.05.006
Abstract
A uniform-sized polymer molecularly imprinted for phenobarbital, which is surface modified by a sugar moiety, has been prepared through a two-step swelling polymerization method using polystyrene beads as seeds, phenobarbital as the template, 4-vinylpyridine as a functional monomer, ethylene glycol dimethacrylate as a cross-linker and 2-O-meth-acryloyloxyethoxyl-(2,3,4,6- tetra-O-acetyl-β-d-galactopyranosyl)-(1–4)-2,3,6-tri-O-acetyl-β-d-glucopyranoside as a surface-modifying glycomonomer, respectively. After deprotecting the glycopolymer, a surface sugar moiety-modified, hydrophilic, molecularly imprinted polymer for phenobarbital (glyco-MIP) was obtained. The resulting polymer beads were packed into a stainless steel column to evaluate their chromatographic characteristics by high-performance liquid chromatography (HPLC). Good selectivity for phenobarbital was obtained with the glyco-MIP compared to the unmodified molecularly imprinted polymer, which revealed that the recognition sites of phenobarbital were unchanged with sugar moiety surface modification. Furthermore, bovine serum albumin was almost completely recovered from the glyco-MIP column, which indicates that the glyco-MIP materials can be used to separate and analyze drugs in complex samples, such as biological samples. The results of pretreatment with and analysis of phenobarbital in serum suggest that this material can be used to analyze phenobarbital in serum through a pretreatment and reverse-phase HPLC analysis process.
Co-reporter:Yanni Zhang, Ying Yang, Tianying Guo
Carbohydrate Polymers 2011 Volume 83(Issue 4) pp:2016-2021
Publication Date(Web):1 February 2011
DOI:10.1016/j.carbpol.2010.11.014
In this work chitosan microspheres have been prepared by ion precipitation using sodium sulfate as precipitant, and crosslinked with natural crosslinker genipin. Then the microspheres were hydrophobically modified by covalent and ion interaction grafting of stearic acid and sodium stearate respectively. Results indicated that the there were still some amount of –NH2 groups on the genipin-crosslinked chitosan microspheres. And the contact angles for hydrophobical graft microspheres were increased greatly from 27° to 104°, which indicates stearic chains have been grafted on the surface of the microspheres even only by a simple graft process based on ion interaction. The interactions of protein with the hydrophobically modified chitosan microspheres were investigated by surface absorption method using bovine serum albumin as a model protein. And results indicated that adsorption efficiency for bovine serum albumin of the fatty chain grafted chitosan microspheres was higher than that of the prestine one distinctly, due to the additional hydrophobic interaction. And the adsorbed protein could well-off be desorbed under a general condition.
Co-reporter:Yong Guo, Ying Yang, Tian Ying Guo
Chinese Chemical Letters 2011 Volume 22(Issue 4) pp:493-496
Publication Date(Web):April 2011
DOI:10.1016/j.cclet.2010.10.050
Firstly, we synthesized N-methacryloyl-histidine monomer and N-methacryloyl-histidine-Cu2+ complex (MAH-Cu2+). Then the molecular imprinting polymers (MIP) has been prepared by surface grafting on uniform polystyrene (PS) core using reversible addition-fragmentation transfer polymerization (RAFT) with MAH-Cu2+ as the functional monomer, methyl paraoxon as the template to simulate phosphotriesterase (PTE). Finally, we have investigated the catalytic hydrolytic activities of MIP and non-imprinting polymers (NIP) to the template methyl paraoxon and the template analogue ethyl paraoxon respectively by UV spectrophotometry. The results showed that the catalytic hydrolytic activity of MIP to the template methyl paraoxon was highest and the value of k is 8.67 × 10−5 mmol L−1 min−1, 3.89-fold higher than MIP to the template analogue ethyl paraoxon, 2.79-fold higher than NIP to the template methyl paraoxon. The KM, rm of MIP are also determined, and KM = 3.95 × 10−4 mol/L, rm = 2.12 μmol/min. The MIP can be reused with only lose 7% of catalytic activity for four cycles.
Co-reporter:Dezhong Zhou, Tianying Guo, Ying Yang, Zhengpu Zhang
Sensors and Actuators B: Chemical 2011 Volume 153(Issue 1) pp:96-102
Publication Date(Web):31 March 2011
DOI:10.1016/j.snb.2010.10.012
Macroporous protein imprinted films (porous-MIP) have been fabricated on the surface of quartz crystal microbalance (QCM) using methyl methacrylate (MMA) as main monomer and trimethylolpropane trimethacrylate (TRIM) as a cross-linker. Macropores were generated by employing calcium carbonate nanoparticles as porogen. The imprinted special surface area and the quantity of the imprinted sites were increased by the formation of macropores on the films. Since the macropores were interconnected in structure, the mass transport and accessibility of protein to the active sites through the material were not affected. In comparison with the nonporous imprinted (nonporous-MIP) films, the porous-MIP films exhibited better affinity and selectivity to template, the binding capacity and mass sensitivity of the biosensor were enhanced. The rigid skeleton structure made the films durable in the recycled tests. The strategy proposed here is generally applicable for constructing high performance biosensor devices.
Co-reporter:Yong Guo;Ying Yang;Lei Zhang;Tian Ying Guo
Macromolecular Research 2011 Volume 19( Issue 11) pp:
Publication Date(Web):2011 November
DOI:10.1007/s13233-011-1107-2
Co-reporter:Tian-Ying Guo;Ping Liu;Yong-Qing Xia ;Mou-Dao Song
Journal of Applied Polymer Science 2010 Volume 116( Issue 3) pp:1611-1616
Publication Date(Web):
DOI:10.1002/app.31525
Abstract
The surface free-radical graft polymerization of 2-O-meth-acryloyloxyethoxyl-(2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl)-(1-4)-2,3,6-tri-O-acetyl-β-D-glucopyranoside onto silica gel particles has been carried out with azobis (isobutyronitrile) as initiator. The grafting reaction conditions and the glycopolymer-grafted silica gel particles have been investigated in detail. Chromatographic experiments have been attempted on glycopolymer-modified silica gel particles as a stationary phase under normal phase conditions, and it was found that a certain separation effect of the quercetin and its derivant isorhamnetin was achieved. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Co-reporter:Yong-Qing Xia;Hai-Li Zhao;Mou-Dao Song;Bang-Hua Zhang;Bao-Long Zhang
Journal of Biomedical Materials Research Part A 2009 Volume 90A( Issue 2) pp:326-332
Publication Date(Web):
DOI:10.1002/jbm.a.32084
Abstract
A novel molecular imprinting method was used to prepare twice-coated silica particles with specific recognition sites for hemoglobin. Chitosan was used as an intermedium to be coated on silica particles via phase inversion process, and the abundance of exposed amine groups (NH2) were active sites for introducing aldehyde groups. After hemoglobin was covalently immobilized by forming imine bonds with the aldehyde groups, acrylamide was then polymerized onto chitosan-coated silica particles to form the recognition sites. The obtained hemoglobin imprinted [molecularly imprinted polymer (MIP)] beads were characterized by FTIR spectroscopy, thermogravimetric analysis (TG), and scanning electron microscopy (SEM). The MIP particles exhibited selectively adsorption for the imprinted protein compared to the nonselectively adsorption for most of proteins of the nonimprinted (NIP) beads. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Co-reporter:Yong-qing Xia;Bang-hua Zhang;Mou-dao Song;Hai-li Zhao;Bao-long Zhang
Journal of Separation Science 2007 Volume 30(Issue 9) pp:1300-1306
Publication Date(Web):20 APR 2007
DOI:10.1002/jssc.200600376
A novel straightforward approach to selective separation for flavonoid compounds was reported. The solid phase material was prepared by copolymerization using allyl-bromide-modified chitosan as macromonomer, and ethylene glycol dimethacrylate as cross-linker. The material was evaluated by chromatographic analysis; it exhibited high selectivity separation for quercetin and its structural analogues using different mobile phases. The material could directly trap a specific class of compounds including quercetin and kaempferol from the hydrolyzate of Ginkgo biloba extract. These results demonstrated the possibility of direct extraction of certain constituents from herb using this material.
Co-reporter:Tianying Guo, Jingwei Zhu, Xi Chen, Moudao Song, Banghua Zhang
Journal of Non-Crystalline Solids 2007 Volume 353(30–31) pp:2893-2899
Publication Date(Web):1 October 2007
DOI:10.1016/j.jnoncrysol.2007.06.023
Novel mesoporous foamed carbons were synthesized from carbonization of organic gels templated by polymer micro-colloidal particles. Resorcinol and formaldehyde were allowed to gel in dilute polymethylmethacrylate (PMMA) microemulsion latex, subsequently the water in the gel was solvent exchanged with methanol and the wet gel was dried under ambient pressure. Pyrolysis was carried out at 800 °C to afford carbon xerogels with porous structures similar to those of resorcinol–formaldehyde (RF) carbon aerogels, but of higher density (>1.2 g/cm3), which provide the carbon materials with relatively higher volumetric surface area (up to 918 m2/cm3). Brunauer–Emmett–Teller (BET) adsorption results indicate that PMMA micro-colloid particles with mean diameter 25 nm contributed to the formation of mesopores of mean diameter at 5 nm.
Co-reporter:Tian-Ying Guo;Jin-Chao Liu;Mou-Dao Song;Bang-Hua Zhang
Journal of Applied Polymer Science 2007 Volume 104(Issue 6) pp:3948-3953
Publication Date(Web):27 MAR 2007
DOI:10.1002/app.26165
Ambient curable carbonyl functional acrylic latices were synthesized by incorporating diacetone acrylamide as functional monomer into acrylic copolymer, adipic acid dihydrazide (ADH) was used as curing agent. In this work acrylic acid (AA) and acrylic acid homopolymer (PAA) were used to facilitate the crosslinking reaction. We found that the properties of latex film were different when use AA and PAA as the source of the carboxyl groups separately. The results from the characterization of carboxyl groups of the latex particles demonstrated that the distribution of the carboxyl group on the latex particle surface is optimal for the crosslinking reaction. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 3948–3953, 2007
Co-reporter:Tian-Ying Guo;Donglin Tang;Moudao Song;Banghua Zhang
Journal of Polymer Science Part A: Polymer Chemistry 2007 Volume 45(Issue 22) pp:5067-5075
Publication Date(Web):1 OCT 2007
DOI:10.1002/pola.22249
Copolymerizations of n-butyl methacrylate (BMA) and fluoro-methacrylates (including 2,2,3,4,4,4-hexafluorobutyl methacrylate, HFBMA and 2,2,2-trifluoroethyl methacrylate, TFEMA) were carried out via reversible addition-fragmentation chain transfer miniemulsion polymerization, using cumyl dithiobenzoate as a chain transfer agent. The experimental results show that the copolymerizations exhibit “living” fashion, with controlled molecular weights and narrow polydispersities. The reactivity ratios of BMA and fluoromethacrylate in this kind of polymerization system were investigated by size exclusion chromatography and nuclear magnetic resonance, from which the Q- and e-values of HFBMA and TFEMA were calculated. Compared with its corresponding non-fluoric methacrylate, fluorinated methacrylate exhibits higher resonance stability of the radical adducts. The Q-value of fluorinated methacrylate is higher (QBMA = 0.82 to QHFBMA = 1.70 and QEMA = 0.76 to QTFEMA = 1.01), and e-value is much larger (eBMA = 0.28 to eHFBMA = 1.24 to and eEMA = 0.17 to eTFEMA = 1.29) for its rather unique high electron-withdrawing inductive effect of the fluoroalkyl ester group. The thermal property and the wetting property of copolymers were also discussed. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 5067–5075, 2007
Co-reporter:Tianying Guo;Xi Chen;Moudao Song;Banghua Zhang
Journal of Applied Polymer Science 2006 Volume 100(Issue 3) pp:1824-1830
Publication Date(Web):25 JAN 2006
DOI:10.1002/app.23081
Structured latex particles with a slightly crosslinked poly(styrene-n-butyl acrylate) (PSB) core and a poly(styrene–methacrylate–vinyl triethoxide silane) (PSMV) shell were prepared by seed emulsion polymerization, and the latex particle structures were investigated with Fourier transform infrared, thermogravimetric analysis, differential scanning calorimetry, transmission electron microscopy, and dynamic light scattering. The films that were formed from the structured core (PSB)–shell (PSMV) particles under ambient conditions had good water repellency and good tensile strength in comparison with films from structured core (PSB)–shell [poly(styrene–methyl methyacrylate)] latex particles; this was attributed to the self-crosslinking of CH2CHSi(OCH2CH3)3 in the outer shell structure. The relationship between the particle structure and the film properties was also investigated in this work. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 1824–1830, 2006
Co-reporter:Tian-Ying Guo, Yong-Qing Xia, Guang-Jie Hao, Bang-Hua Zhang, Guo-Qi Fu, Zhi Yuan, Bing-Lin He, John F. Kennedy
Carbohydrate Polymers 2005 Volume 62(Issue 3) pp:214-221
Publication Date(Web):1 December 2005
DOI:10.1016/j.carbpol.2005.03.012
A simple molecularly imprinted polymer (MIP) was prepared using hemoglobin as the imprinted molecule and acrylamide as the functional monomer. The MIP was achieved by grafting of the selective soft polyacrylamide gel to the maleic anhydride modified chitosan beads by letting the monomers and the protein diffuse into the pores of the chemically modified chitosan matrix before starting the polymerization. The chitosan beads were freed from the surrounding polyacrylamide gel by washing. Langmuir analysis showed that an equal class of adsorption was formed in the MIP, the adsorption equilibrium constant and the maximum adsorption capacity were evaluated to be 1.47 and 35.7 mg/g wet MIP beads, respectively. The MIP has much higher adsorption capacity for hemoglobin than the non-imprinted polymer with the same chemical composition, and also has a higher selectivity for the imprinted molecule. The MIP can be reused and the reproducibility was approximately 100% at low concentration.
Co-reporter:Tian Ying Guo;Chen Xi;Guang Jie Hao;Mou Dao Song;Bang Hua Zhang
Advances in Polymer Technology 2005 Volume 24(Issue 4) pp:
Publication Date(Web):23 NOV 2005
DOI:10.1002/adv.20051
Self-crosslinking poly(methyl methacrylate-co-n-butyl acrylate-co-styrene-co-vinyltriethoxysilane) latex particles were prepared by seed emulsion polymerization. The presence of SiOSi in the prepared copolymer latex particle was confirmed by FT-IR. The crosslinking degree of the polymer latex particle was investigated by using tetrahydrofuran to swell the dried copolymer films with different vinyltriethoxysilane contents. The thermal properties of the solid films were investigated by using DSC and TGA. In addition, the mechanical properties and water resistances of the different copolymer films were also investigated in detail. It was found that the mechanical properties of the copolymer film could be enhanced by introducing vinyltriethoxysilane, and the water resistance of the copolymer film could be improved at certain vinyltriethoxysilane contents. © 2005 Wiley Periodicals, Inc. Adv Polym Techn 24: 288–295, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/adv.20051
Co-reporter:Tianying Guo;Guangjie Hao;Moudao Song;Banghua Zhang
Journal of Applied Polymer Science 2004 Volume 94(Issue 1) pp:
Publication Date(Web):29 JUL 2004
DOI:10.1002/app.20867
Amphiphilic poly(styrene-b-ethylene oxide) block copolymers were synthesized by sequential anionic copolymerization. Layered silicates intercalated by the block copolymers were prepared via melt intercalation. The effects of the intercalated clay on an acrylonitrile–butadiene–styrene resin were characterized with X-ray diffraction, transmission electron microscopy, stress–strain measurements in elongation, and thermogravimetric analysis. X-ray and microscopy results demonstrated that the main structure of the dispersed block-copolymer-intercalated clay in the acrylonitrile–butadiene–styrene resin matrix was mono- and bundle- layered. Further studies demonstrated that the resin showed considerable improvement in its tensile strength, modulus, and thermal stability. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 238–242, 2004
Co-reporter:G. X. Chen;G. J. Hao;T. Y. Guo;M. D. Song;B. H. Zhang
Journal of Applied Polymer Science 2004 Volume 93(Issue 2) pp:655-661
Publication Date(Web):22 APR 2004
DOI:10.1002/app.20512
The preparation and properties of nanocomposites, consisting of a poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) and an organophilic clay are described. The effect of organophilic clay on the crystallization behavior of (PHBV) was studied. A differential scanning calorimeter (DSC) was used to monitor the energy of the crystallization process from the melt. During the crystallization process from the melt, the organophilic clay led to an increase in crystallization temperature (Tc) of PHBV compared with that for plain PHBV. During isothermal crystallization, dependence of the relative degree of crystallization on time was described by the Avrami equation. The addition of organophilic clay caused an increase in the overall crystallization rate of PHBV, but did not influence the mechanism of nucleation, and growth of the PHBV crystals and the increase caused by a small quantity of clay is move effective than that large one. The equilibrium melting temperature of PHBV was determined as 186°C. Analysis of kinetic data according to nucleation theories showed that the increase in crystallization rate of PHBV in the composite is due to the decrease in surface energy of the extremity surface. The mechanical test shows that the tensile strength of hybrid increased to 35.6 MPa, which is about 32% higher than that of the original PHBV with the incorporation of 3 wt % clay, and the tensile modulus was also increased. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 655–661, 2004
Co-reporter:T. Y. Guo;G. L. Tang;G. J. Hao;M. D. Song;B. H. Zhang
Journal of Applied Polymer Science 2003 Volume 90(Issue 5) pp:1290-1297
Publication Date(Web):27 AUG 2003
DOI:10.1002/app.12752
Core–shell structured particles, which comprise the rubbery core and glassy layers, were prepared by emulsifier-free emulsion polymerization of poly(n-butyl acrylate/methyl methacrylate)/polystyrene [P(n-BA/MMA)/PS]. The particle diameter was about 0.22 μm, and the rubbery core was uncrosslinked and lightly crosslinked, respectively. The smaller core–shell structured particle–toughened PS blends were investigated in detail. The dynamic mechanical behavior and observation by scanning electron microscopy of the modified blend system with core–shell structured particles indicated good compatibility between PS and the particles, which is the necessary qualification for an effective toughening modifier. Notched-impact strength and related mechanical properties were measured for further evaluation of the toughening efficiency. The notched-impact strength of the toughened PS blends with uncrosslinked particles reached almost sixfold higher than that of the untoughened PS when 15 phr of the core–shell structured particles was added. For the crosslinked particles the toughening effect for PS was not obvious. The toughening mechanism for these smaller particles also is discussed in this article. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 1290–1297, 2003
Co-reporter:Yong-qing Xia, Tian-ying Guo, Mou-dao Song, Bang-hua Zhang, Bao-long Zhang
Reactive and Functional Polymers (January 2008) Volume 68(Issue 1) pp:63-69
Publication Date(Web):January 2008
DOI:10.1016/j.reactfunctpolym.2007.10.018
Co-reporter:Shuai Liu, Zhibing Sun, Dezhong Zhou and Tianying Guo
Journal of Materials Chemistry A 2017 - vol. 5(Issue 27) pp:NaN5310-5310
Publication Date(Web):2017/06/16
DOI:10.1039/C7TB00996H
A branched poly(β-amino ester) with numerous alkyl chains (BPA) is designed and synthesized as a safe and efficient non-viral vector. The branching and hydrophobicity synergistically endow BPA with tight DNA condensation, high polyplex stability in serum, high cellular uptake and ultimately robust gene transfection efficiency, largely superior to its linear counterpart (LPA). Our results demonstrate that branching matters for gene delivery by hydrophobic gene vectors.
Co-reporter:Dezhong Zhou, Congxin Li, Yuling Hu, Hao Zhou, Jiatong Chen, Zhengpu Zhang and Tianying Guo
Chemical Communications 2012 - vol. 48(Issue 38) pp:NaN4596-4596
Publication Date(Web):2012/03/15
DOI:10.1039/C2CC31359F
A novel multifunctional oligomer is synthesized and incorporated to enhance the gene delivery efficiency of PLL via non-electrostatic assembly and covalent grafting strategies. The improvement of the gene delivery efficiency is dependent on the gene carrying complex properties, and the properties are dependent on the oligomer incorporation strategy.
Co-reporter:Wenhao Chi, Shuai Liu, Jixiang Yang, Ruiyu Wang, Hongqi Ren, Hao Zhou, Jiatong Chen and Tianying Guo
Journal of Materials Chemistry A 2014 - vol. 2(Issue 33) pp:NaN5396-5396
Publication Date(Web):2014/06/12
DOI:10.1039/C4TB00807C
Two kinds of novel oligomers were prepared by reversible addition fragmentation chain transfer (RAFT) polymerization and incorporated into the polyethyleneimine (PEI) gene delivery system through non-electrostatic assembly to improve gene transfection efficiency. The non-electrostatic assembly process was first investigated via probing the interactions of the oligomers with plasmid DNA (pDNA), PEI and AD-293 cells using a quartz crystal microbalance (QCM). The results show that the prepared oligomers almost had no interaction with pDNA while they had much stronger interactions with PEI and AD-293 cells. Meanwhile, we found that the two kinds of oligomers had different interactions with AD-193 cells, which caused different effects on gene transfection. The data of QCM tests combined with the in vitro transfection results can be used to explain what effects the oligomers have on improving gene transfection. The results also indicate that the strategy of detecting the interactions of oligomers with pDNA, polycations and cells will contribute to predetermine whether the prepared oligomer is efficient in improving gene transfection.
Co-reporter:Shuai Liu, Jixiang Yang, Hongqi Ren, Jonathan O'Keeffe-Ahern, Dezhong Zhou, Hao Zhou, Jiatong Chen and Tianying Guo
Biomaterials Science (2013-Present) 2016 - vol. 4(Issue 3) pp:NaN532-532
Publication Date(Web):2016/01/21
DOI:10.1039/C5BM00530B
Natural polycations, such as poly(L-lysine) (PLL) and chitosan (CS), have inherent superiority as non-viral vectors due to their unparalleled biocompatibility and biodegradability. However, the application was constrained by poor transfection efficiency and safety concerns. Since previous modification strategies greatly weakened the inherent advantages of natural polycations, developing a strategy for functional group introduction with broad applicability to enhance the transfection efficiency of natural polycations without compromising their cationic properties is imperative. Herein, two uncharged functional diblock oligomers P(DMAEL-b-NIPAM) and P(DMAEL-b-Vlm) were prepared from a lactose derivative, N-iso-propyl acrylamide (NIPAM) as well as 1-vinylimidazole (Vlm) and further functionalized with four small ligands folate, glutathione, cysteine and arginine, respectively, aiming to enhance the interactions of complexes with cells, which were quantified utilizing a quartz crystal microbalance (QCM) biosensor, circumventing the tedious material screening process of cell transfection. Upon incorporation with PLL and DNA, the multifunctional oligomers endow the formulated ternary complexes with great properties suitable for transfection, such as anti-aggregation in serum, destabilized endosome membrane, numerous functional sites for promoted endocytosis and therefore robust transfection activity. Furthermore, different from the conventional strategy of decreasing cytotoxicity by reducing the charge density, the multifunctional oligomer incorporation strategy maintains the highly positive charge density, which is essential for efficient cellular uptake. This system develops a new platform to modify natural polycations towards clinical gene therapy.
Co-reporter:Yong Guo and Tianying Guo
Chemical Communications 2013 - vol. 49(Issue 11) pp:NaN1075-1075
Publication Date(Web):2012/12/12
DOI:10.1039/C2CC38022F
A new strategy of reactant–product-dual-template imprinting incorporated with hollow morphology to improve the catalysis efficiency of microspheres was developed. The synthesized capsule can achieve degradation of the reactant and elimination of its product simultaneously.
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