Co-reporter:Yin Liu, Gaoxing Su, Fei Wang, Jianbo Jia, Shuhuan Li, Linlin Zhao, Yali Shi, Yaqi Cai, Hao Zhu, Bin Zhao, Guibin Jiang, Hongyu Zhou, and Bing Yan
Environmental Science & Technology June 20, 2017 Volume 51(Issue 12) pp:7120-7120
Publication Date(Web):May 24, 2017
DOI:10.1021/acs.est.7b01635
Perfluorooctanesulfonate (PFOS) persistently accumulates in the environment and in humans, causing various toxicities. To determine the key molecular determinants for optimal PFOS specificity and efficiency, we designed and synthesized a combinatorial gold nanoparticle (GNP) library consisting of 18 members with rationally diversified hydrophobic, electrostatic, and fluorine–fluorine interaction components for PFOS bindings. According to our findings, the electrostatic and F–F interactions between PFOS and nanoparticles are complementary. When F–F attractions are relatively weak, the electrostatic interactions are dominant. As F–F interactions increase, the electrostatic contributions are reduced to as low as 20%, demonstrating that F–F binding may overpower even electrostatic interactions. Furthermore, F–F interactions (28–79% binding efficiency) are 2-fold stronger than regular hydrophobic interactions (15–39% binding efficiency) for PFOS adsorption, explaining why these novel PFOS-binding nanoparticles are superior to other conventional materials based on either hydrophobic or electrostatic binding. The PFOS adsorption by the optimized nanoparticles performs well in the presence of ionic interferences and in environmental wastewater. This library mapping approach can potentially be applied to recognition mechanism investigation of other pollutants and facilitate the discovery of effective monitoring probes and matrices for their removal.
Co-reporter:Jianbo Jia;Sijin Liu;Hongyu Zhou;Guibin Jiang;Shumei Zhai;Feifei Li
Environmental Science & Technology February 7, 2017 Volume 51(Issue 3) pp:1775-1784
Publication Date(Web):January 9, 2017
DOI:10.1021/acs.est.6b05200
Co-reporter:Jianbo Jia, Feifei Li, Hongyu Zhou, Yuhong Bai, Sijin Liu, Yiguo Jiang, Guibin Jiang, and Bing Yan
Environmental Science & Technology August 15, 2017 Volume 51(Issue 16) pp:9334-9334
Publication Date(Web):July 19, 2017
DOI:10.1021/acs.est.7b02752
As the applications and environmental release of silver ions and nanoparticles are increasing, increasing human exposure to these pollutants has become an emerging health concern. The impeding effects of such pollutants on susceptible populations are severely under-studied. Here, we demonstrate that silver nanoparticles (Ag NPs), at a dose that causes no general toxicity in normal mice, promotes the progression of fatty liver disease from steatosis to steatohepatitis only in overweight mice. Exposure to Ag+ ions induces the same effects in overweight mice. Ag NPs rather than Ag+ ions cause this disease progression based on our findings that Ag+ ions are partly reduced to Ag NPs in fatty livers, and the toxic effect is correlated with the liver dose of Ag NPs, not Ag+ ions. Furthermore, the Ag NP-induced pro-inflammatory activation of Kupffer cells in the liver, enhancement of hepatic inflammation, and suppression of fatty acid oxidation are identified as key factors in the underlying mechanisms.
Co-reporter:Gaoxing Su;Huaqiao Jiang;Hongyan Zhu;Jing-Jing Lv;Guohai Yang;Jun-Jie Zhu
Nanoscale (2009-Present) 2017 vol. 9(Issue 34) pp:12494-12502
Publication Date(Web):2017/08/31
DOI:10.1039/C7NR04046F
Plasmonic Au–Pd nanostructures have drawn significant attention for use in heterogeneous catalysis. In this study, palladium nanodendrite-tipped gold nanorods (PdND-T-AuNRs) were subjected to a facile fabrication under mild reaction conditions. The palladium amounts on the two tips were tunable. In the preparation of PdND-T-AuNRs, dense capped AuNRs, a low reaction temperature, and suitable stabilizing agents were identified as critical reaction parameters for controlling palladium nanodendrites deposited on both ends of AuNRs. After overgrowth with palladium nanodendrites, the longitudinal surface plasmonic resonance peaks of PdND-T-AuNRs were red-shifted from 810 nm to 980 nm. The electrocatalytic activity of PdND-T-AuNRs for ethanol oxidation was examined, which was a bit weaker than that of cuboid core–shell Au–Pd nanodendrites; however, PdND-T-AuNRs were more stable in ethanol electrooxidation. Moreover, the photocatalytic activity of PdND-T-AuNRs for Suzuki cross-coupling reactions was investigated. At room temperature, nearly 100% yield was obtained under laser irradiation. The results can further enhance our capability of fine-tuning the optical, electronic, and catalytic properties of the bimetallic Au–Pd nanostructures.
Co-reporter:Bing Yan, Qiuquan Wang, Guibin Jiang
NanoImpact 2017 Volume 7(Volume 7) pp:
Publication Date(Web):1 July 2017
DOI:10.1016/j.impact.2017.04.002
Co-reporter:Yi Zhang;Yabin Wang;Aijuan Liu;Sherry Li Xu;Bin Zhao;Yi Zhang;Hanfa Zou;Wenyi Wang;Hao Zhu
Advanced Functional Materials 2016 Volume 26( Issue 6) pp:841-850
Publication Date(Web):
DOI:10.1002/adfm.201504182
The liver plays an important role in metabolizing foreign materials, such as drugs. The high accumulation of carbon nanotubes and other hydrophobic nanoparticles in the liver has raised concerns that nanoparticles may interfere with liver metabolic function. We report here that carbon nanotubes enter hepatic cells after intravenous introduction and interact with CYP enzymes, including CYP3A4. Surface chemical modifications alter the carbon nanotubes' interactions with CYP450 enzymes in human liver microsomes. They enhance, inhibit, or have no effect on the enzymatic function of CYP3A4. Using a cheminformatics analysis, certain chemical structures are identified on the surface of the carbon nanotubes that induce an enzyme inhibitory effect or prevent disruption of CYP3A4 enzymes.
Co-reporter:Peifu Jiao, Mario Otto, Qiaohong Geng, Chencan Li, Faming Li, Elizabeth R. Butch, Scott E. Snyder, Hongyu Zhou and Bing Yan
Journal of Materials Chemistry A 2016 vol. 4(Issue 3) pp:513-520
Publication Date(Web):07 Dec 2015
DOI:10.1039/C5TB02243F
Although nanomaterials have been widely investigated for drug delivery, imaging and immunotherapy, their potential roles in triggering innate cellular immune responses while simultaneously serving as imaging enhancer remain unexplored. In this work, gold nanoparticles (GNPs) conjugated to the tumor-targeting anti-GD2 antibody hu14.18K322A, namely HGNPs, were designed and synthesized to specifically enhance computerized tomography (CT) imaging contrast and to stimulate the attack of neuroblastoma and melanoma cells by natural killer (NK) cells. The HGNPs specifically targeted GD2-positive neuroblastoma (NB1691) and melanoma (M21) cells, with an enhancement of CT contrast images of the HGNP-labeled cell pellets by 5.27- and 7.66-fold, respectively, compared to images of unlabeled cell pellets. The HGNPs also triggered NK-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) in NB1691 and M21 cells with a two-fold higher efficacy compared to that elicited by hu14.18K322A alone, with no adverse effect to GD2-negative PC-3 cells. These results suggest that HGNPs are promising theranostic agents for neuroblastoma and melanoma cancers.
Co-reporter:Gaoxing Su, Xiaofei Zhou, Hongyu Zhou, Ye Li, Xianren Zhang, Yin Liu, Dapeng Cao, and Bing Yan
ACS Applied Materials & Interfaces 2016 Volume 8(Issue 44) pp:30037
Publication Date(Web):October 17, 2016
DOI:10.1021/acsami.6b10967
Understandings of how biomolecules modify nanoparticles in a biological context and how these exchanges impact nano-biointeractions are fundamental to nanomedicine and nanotoxicology research. In this work, cancer-targeting gold nanoparticles (TGNPs) with different sizes (5, 15, and 40 nm) were designed and synthesized. These nanoparticles spontaneously adsorbed proteins in complete cell culture medium (Dulbecco’s modified Eagle’s medium with 10% human serum). Although the targeting ligands on the surface of nanoparticles were likely to be shielded by adsorbed proteins, the targeting capability of nanoparticles was maintained due to the highly dynamic nature of protein adsorption. By regulating the size and surface curvature of nanoparticles, we found that smaller TGNPs (5 nm, large surface curvature) recognize folate receptors on HeLa cells mainly through one-on-one bindings, and adsorbed proteins partially interfered with their binding, inducing a reduction of cell uptake by ∼30%. Larger TGNPs (40 nm, small surface curvature) bound to cell surface receptors through multivalent interactions, and their binding affinity was, in contrast, enhanced by adsorbed proteins, resulting in an increased cell uptake by ∼13%. Computational modeling further corroborated our experimental findings. The compelling findings from this work demonstrated how nanoparticle’s size controlled its biological activity and provided key design principles for nanomedicine agents.Keywords: cell recognition; cell targeting; delivery; nanomedicine; protein adsorption
Co-reporter:Y Mu, Y Liu, J Xiang, Q Zhang, S Zhai, D P Russo, H Zhu, X Bai and B Yan
Cell Death & Disease 2016 7(3) pp:e2143
Publication Date(Web):2016-03-01
DOI:10.1038/cddis.2016.53
A novel tricyclic thiazepine derivative, 6-(p-tolyl)benzo[f] pyrido[2,3-b][1,4] thiazepine 11,11-dioxide (TBPT), exhibits potent inhibitory effects in two non-small-cell lung cancer cell lines, H460 and its drug-resistant variant, H460TaxR, while exhibiting much less toxic effects on normal human fibroblasts. After five injections of TBPT at a dose of 60 mg/kg, it inhibits H460TaxR tumor growth in xenografted mouse models by 66.7% without causing observable toxicity to normal tissues. Based on gene perturbation data and a series of investigations, we reveal that TBPT is not a P-glycoprotein substrate and it inhibits microtubule formation by targeting tubulin, thereby causing cell cycle arrest at the G2/M stage and eventually inducing apoptosis. This redeployment of anti-depressant compound scaffold for anticancer applications provides a promising future for conquering drug-resistant tumors with fewer side effects.
Co-reporter:Jinbao Xiang, Zhuoqi Zhang, Yan Mu, Xianxiu Xu, Sigen Guo, Yongjin Liu, Daniel P. Russo, Hao Zhu, Bing Yan, and Xu Bai
ACS Combinatorial Science 2016 Volume 18(Issue 5) pp:230
Publication Date(Web):April 15, 2016
DOI:10.1021/acscombsci.6b00010
An efficient discovery strategy by combining diversity-oriented synthesis and converging cellular screening is described. By a three-round screening process, we identified novel tricyclic pyrido[2,3-b][1,4]benzothiazepines showing potent inhibitory activity against paclitaxel-resistant cell line H460TaxR (EC50 < 1.0 μM), which exhibits much less toxicity toward normal cells (EC50 > 100 μM against normal human fibroblasts). The most active hits also exhibited drug-like properties suitable for further preclinical research. This redeployment of antidepressing compounds for anticancer applications provides promising future prospects for treating drug-resistant tumors with fewer side effects.Keywords: antidepressing; H460TaxR; redeployment; selective cytotoxicity; tricyclic thiazepine
Co-reporter:Yi Zhang, Ling Wu, Cuijuan Jiang, and Bing Yan
Chemical Research in Toxicology 2015 Volume 28(Issue 3) pp:296
Publication Date(Web):December 23, 2014
DOI:10.1021/tx500480d
Nanoparticles, such as carbon nanotubes (CNTs), interact with cells and are easily internalized, causing various perturbations to cell functions. The mechanisms involved in such perturbations are investigated by a systematic approach that utilizes modified CNTs and various chemical–biological assays. Three modes of actions are (1) CNTs bind to different cell surface receptors and perturb different cell signaling pathways; (2) CNTs bind to a receptor with different affinity and, therefore, strengthen or weaken signals; (3) CNTs enter cells and bind to soluble signaling proteins involved in a signaling pathway. Understanding of such mechanisms not only clarifies how CNTs cause cytotoxicity but also demonstrates a useful method to modulate biological/toxicological activities of CNTs for their various industrial, biomedical, and consumer applications.
Co-reporter:Yin Liu, Fei Li, Ling Wu, Wenyi Wang, Hao Zhu, Qiu Zhang, Hongyu Zhou, Bing Yan
Bioorganic & Medicinal Chemistry Letters 2015 Volume 25(Issue 9) pp:1971-1975
Publication Date(Web):1 May 2015
DOI:10.1016/j.bmcl.2015.03.016
Co-reporter:Shuhuan Li, Shumei Zhai, Yin Liu, Hongyu Zhou, Jinmei Wu, Qing Jiao, Bin Zhang, Hao Zhu, Bing Yan
Biomaterials 2015 52() pp: 312-317
Publication Date(Web):
DOI:10.1016/j.biomaterials.2015.02.043
Co-reporter:Qingxin Mu, Guibin Jiang, Lingxin Chen, Hongyu Zhou, Denis Fourches, Alexander Tropsha, and Bing Yan
Chemical Reviews 2014 Volume 114(Issue 15) pp:7740
Publication Date(Web):June 13, 2014
DOI:10.1021/cr400295a
Co-reporter:Yi Zhang, Yuhong Bai, Jianbo Jia, Ningning Gao, Yang Li, Ruinan Zhang, Guibin Jiang and Bing Yan
Chemical Society Reviews 2014 vol. 43(Issue 10) pp:3762-3809
Publication Date(Web):19 Mar 2014
DOI:10.1039/C3CS60338E
Nanotechnology is having a tremendous impact on our society. However, societal concerns about human safety under nanoparticle exposure may derail the broad application of this promising technology. Nanoparticles may enter the human body via various routes, including respiratory pathways, the digestive tract, skin contact, intravenous injection, and implantation. After absorption, nanoparticles are carried to distal organs by the bloodstream and the lymphatic system. During this process, they interact with biological molecules and perturb physiological systems. Although some ingested or absorbed nanoparticles are eliminated, others remain in the body for a long time. The human body is composed of multiple systems that work together to maintain physiological homeostasis. The unexpected invasion of these systems by nanoparticles disturbs normal cell signaling, impairs cell and organ functions, and may even cause pathological disorders. This review examines the comprehensive health risks of exposure to nanoparticles by discussing how nanoparticles perturb various physiological systems as revealed by animal studies. The potential toxicity of nanoparticles to each physiological system and the implications of disrupting the balance among systems are emphasized.
Co-reporter:Liwen Li, Yin Liu, Qiu Zhang, Hongyu Zhou, Yi Zhang, and Bing Yan
ACS Chemical Biology 2014 Volume 9(Issue 3) pp:731
Publication Date(Web):December 30, 2013
DOI:10.1021/cb4005589
Using a tubulin polymerization inhibitor and a tubulin polymerization/Dyrk1B dual inhibitor, we intentionally allowed or blocked the Dyrk1B-coordinated cell survival process in response to microtubule damage. By examining the resulting differential effects on cell function and phenotype, we have elucidated key molecular interactions involved in the Dyrk1B-coordinated cell survival process as well as the associated overall cellular impact. Dyrk1B activation that is induced by microtubule damage triggers microtubule stabilization and promotes the mitochondrial translocation of p21Cip1/waf1 (referred to as p21 hereafter) to suppress apoptosis. These coordinated survival events rapidly repair microtubules, relieve cell G2/M arrest for 42% of the cells, suppress apoptosis for 27% of the cells, and increase cell viability by 10-fold. That is, the dual inhibitor is 10 times more potent in the inhibition of cancer cell viability. This approach affords a novel drug discovery strategy by targeting both the therapeutic targets and the associated cell survival pathway using a single therapeutic agent.
Co-reporter:Ruinan Zhang, Xiaohong Pan, Fei Li, Lin Zhang, Shumei Zhai, Qingxin Mu, Jingfu Liu, Guangbo Qu, Guibin Jiang, and Bing Yan
Environmental Science & Technology 2014 Volume 48(Issue 3) pp:1984-1992
Publication Date(Web):January 6, 2014
DOI:10.1021/es404934f
Some nanomaterials, such as Mg(OH)2 nanoflakes, are heavily used in pollutant adsorption and removal. Residues from these environmental remediations are potential hazardous materials. Safety evaluations of these materials are needed for environmental protection and human health. Although nanotoxicity has been widely investigated in recent years, research on the toxicity of nanoparticle/pollutant adducts has been rather inadequate. Here, we report the cellular perturbations and cytotoxicity of nano-Mg(OH)2/Cr(VI) adducts as a case study to elucidate how nanoparticle/pollutant adducts impact human cells. We found that Mg(OH)2 nanoflakes barely enter cells, while desorbed Cr(VI) anions enter cells, generate ROS, induce cell apoptosis, and cause cytotoxicity. This cytotoxicity is only a fraction of the cytotoxicity of free Cr(VI) because nano-Mg(OH)2 particles are able to retain more than half of their Cr(VI) anions.
Co-reporter:C Zhang, S Zhai, X Li, Q Zhang, L Wu, Y Liu, C Jiang, H Zhou, F Li, S Zhang, G Su, B Zhang and B Yan
Cell Death & Disease 2014 5(3) pp:e1138
Publication Date(Web):2014-03-01
DOI:10.1038/cddis.2014.76
By screening a collection of one hundred combinations of thiazolidinone compounds, we identified one combination (M4) that synergistically inhibited the growth of H460 and H460/TaxR cells and tumor growth in H460/TaxR xenograft mice. A whole genome microarray assay showed that genes involved in negative regulation of microtubule polymerization or depolymerization, intracellular protein kinase cascade, positive regulation of histone acetylation, cell cycle arrest and apoptosis were upregulated. Further analysis proved that the four compounds act as either microtubule polymerization inhibitors or histone deacetylase inhibitors. They act synergistically targeting multiple proteins and leading to the regulation of cell cycle checkpoint proteins, including p53, p21, cdc25C and cdc2, the activation of caspases, JNK, p38 cascades and the inactivation of Akt. These events resulted in the G2/M cell cycle arrest and cell apoptosis. These data provide a new strategy for discovering anticancer drugs and drug combinations for drug-resistant cancers.
Co-reporter:Ling Wu, Yi Zhang, Chengke Zhang, Xuehui Cui, Shumei Zhai, Yin Liu, Changlong Li, Hao Zhu, Guangbo Qu, Guibin Jiang, and Bing Yan
ACS Nano 2014 Volume 8(Issue 3) pp:2087-2099
Publication Date(Web):February 19, 2014
DOI:10.1021/nn500376w
The induction of autophagy by nanoparticles causes nanotoxicity, but appropriate modulation of autophagy by nanoparticles may have therapeutic potential. Multiwalled carbon nanotubes (MWCNTs) interact with cell membranes and membrane-associated molecules before and after internalization. These interactions alter cellular signaling and impact major cell functions such as cell cycle, apoptosis, and autophagy. In this work, we demonstrated that MWCNT-cell interactions can be modulated by varying densely distributed surface ligands on MWCNTs. Using a fluorescent autophagy-reporting cell line, we evaluated the autophagy induction capability of 81 surface-modified MWCNTs. We identified strong and moderate autophagy-inducing MWCNTs as well as those that did not induce autophagy. Variation of the surface ligand structure of strong autophagy nanoinducers led to the induction of different autophagy-activating signaling pathways, presumably through their different interactions with cell surface receptors.Keywords: autophagy; carbon nanotube; combinatorial library; mTOR; signaling pathway; surface modification;
Co-reporter:Hongyu Zhou, Yi Zhang, Gaoxing Su, Shumei Zhai and Bing Yan
RSC Advances 2013 vol. 3(Issue 44) pp:21596-21603
Publication Date(Web):02 Sep 2013
DOI:10.1039/C3RA43838D
In this report, a cancer-targeting nanoconstruct enters a cancer cell four times more readily than non-targeting nanoparticles. Inside the cell, the nanoconstruct conducts a double DNA attack from a DNA intercalating drug doxorubicin and radiation-enhancing gold nanoparticles achieving 2.5 fold higher DNA double strand breakage in the cancer genome. An overall 4.0 or 2.7-fold increase in cancer cell killing was achieved compared to the free drug without or with X-ray irradiation and a significant reduction in nonspecific toxicity to folate receptor-negative cells demonstrated the unique advantage of a nanomedicine approach to future chemotherapy and radiotherapy.
Co-reporter:Aijuan Liu, Shumei Zhai, Bin Zhang, Bing Yan
TrAC Trends in Analytical Chemistry 2013 Volume 48() pp:1-13
Publication Date(Web):July–August 2013
DOI:10.1016/j.trac.2013.03.005
•Tracking SWCNTs in vivo using their own optical properties in the near infrared.•Tracking MWCNTs, graphene and fullerene using magnetic or fluorescent labels.•Radioactive labels are powerful for tracking carbon nanoparticles in vivo.Monitoring carbon nanoparticles (CNPs) in vivo is still a great challenge. Employing the intrinsic properties of single-walled carbon nanotubes, near-infrared fluorescence, Raman and photoacoustic imaging are the preferred methods for tracking CNPs in vivo. For other carbon nanomaterials, labeling with magnetic, fluorescent or radioactive agents is required to achieve the same goal.
Co-reporter:Yi Zhang, Samuel Tekobo, Ying Tu, Qunfang Zhou, Xinlong Jin, Sergey A. Dergunov, Eugene Pinkhassik, and Bing Yan
ACS Applied Materials & Interfaces 2012 Volume 4(Issue 8) pp:4099
Publication Date(Web):July 27, 2012
DOI:10.1021/am300840p
Changing polystyrene nanoparticles from three-dimensional spherical shape to two-dimensional disk shape promotes their cell surface binding with significant reduction of cell uptake. As a result of lower cell uptake, nanodisks show very little perturbations on cell functions such as cellular ROS generation, apoptosis and cell cycle progression compared to nanospheres. Therefore, disk-shaped nanoparticles may be a promising template for developing cell membrane-specific and safer imaging agents for a range of biomedical applications such as molecular imaging, tissue engineering, cell tracking, and stem cell separation.Keywords: cell uptake; cytotoxicity; membrane permeability; nanodisk;
Co-reporter:Yongyi Wei, Yang Li, Jianbo Jia, Yiguo Jiang, Bin Zhao, Qiu Zhang, Bing Yan
NanoImpact (July 2016) Volumes 3–4() pp:1-11
Publication Date(Web):1 July 2016
DOI:10.1016/j.impact.2016.09.003
•More liver deposition of ZnO NPs in aged mice compared with young after oral exposures•ZnO NPs induced liver injury only in aged mice but not in young mice.•More hepatic oxidative stress and inflammation found in aged mice•Aged mice are more vulnerable to oral exposures to ZnO NPs.The potential toxic effects of nanoparticles (NPs) might be more severe in susceptible populations such as the elderly. To confirm such effects, we built an aged mouse model and evaluated the effects after giving model mice oral doses of zinc oxide (ZnO) NPs. We observed enhanced ZnO NP absorption in aged mice compared with young mice most likely because of an age-related increase in intestinal permeability. The liver deposition of ZnO NPs was higher in aged mice compared with young mice. As a result, liver injuries were observed in aged mice, whereas young mice were not affected. ZnO NPs induced increases in oxidative stress and inflammation levels in both aged and young mice. Because aged mice have already endured age-related increases in oxidative stress and inflammation, ZnO NPs caused additional damage and resulted in acute liver injury. The above evidence suggests that the elderly are more vulnerable to NP exposure, and more caution should be taken with regard to the application of or accidental exposure to ZnO NPs among the elderly.Download high-res image (124KB)Download full-size image
Co-reporter:Zhiping Wang, Chunhui Li, Yan Mu, Zhang Lin, Anji Yi, Qiu Zhang, Bing Yan
Journal of Hazardous Materials (28 April 2015) Volume 287() pp:296-305
Publication Date(Web):28 April 2015
DOI:10.1016/j.jhazmat.2015.02.005
•The nano-Mg(OH)2/Cr(VI) adduct formation altered biodistribution of Cr(VI) in dam.•Alterations in placenta and hormones play a role in Cr(VI)-induced fetotoxicity.•The nanoadduct formation reduced fetotoxicity of Cr(VI) anions.During pregnancy, both the mother and fetus are vulnerable to environmental pollution by particulate matters and chemicals. Although the toxicity of free pollutants has been frequently reported, the impact of nanoparticle/pollutant adducts on the vulnerable pregnant population remains unclear. In this study, pregnant mice were orally exposed to Mg(OH)2 nanoflakes and nanoflakes adsorbed with Cr(VI) anions during the peri-implantation and organogenesis stages of pregnancy at doses that did not induce systemic toxicity or pregnancy complications. The nano-Mg(OH)2/Cr(VI) adducts formation reduced fetal developmental toxicity compared with the toxicity induced by the same concentration of free Cr(VI) anions.
Co-reporter:Xue Bai, Fang Liu, Yin Liu, Cong Li, Shenqing Wang, Hongyu Zhou, Wenyi Wang, Hao Zhu, David A. Winkler, Bing Yan
Toxicology and Applied Pharmacology (15 May 2017) Volume 323() pp:66-73
Publication Date(Web):15 May 2017
DOI:10.1016/j.taap.2017.03.011
•Nanomaterials studies make non-systematic alterations to nanoparticle properties.•Vast nanomaterials property spaces require systematic studies of nano-bio interactions.•Experimental design and modelling are efficient ways of exploring materials spaces.•We advocate systematic modification and computational analysis to probe nano-bio interactions.Many studies of nanomaterials make non-systematic alterations of nanoparticle physicochemical properties. Given the immense size of the property space for nanomaterials, such approaches are not very useful in elucidating fundamental relationships between inherent physicochemical properties of these materials and their interactions with, and effects on, biological systems. Data driven artificial intelligence methods such as machine learning algorithms have proven highly effective in generating models with good predictivity and some degree of interpretability. They can provide a viable method of reducing or eliminating animal testing. However, careful experimental design with the modelling of the results in mind is a proven and efficient way of exploring large materials spaces. This approach, coupled with high speed automated experimental synthesis and characterization technologies now appearing, is the fastest route to developing models that regulatory bodies may find useful. We advocate greatly increased focus on systematic modification of physicochemical properties of nanoparticles combined with comprehensive biological evaluation and computational analysis. This is essential to obtain better mechanistic understanding of nano-bio interactions, and to derive quantitatively predictive and robust models for the properties of nanomaterials that have useful domains of applicability.Download high-res image (251KB)Download full-size image
Co-reporter:Yi Zhang, Yuhong Bai, Jianbo Jia, Ningning Gao, Yang Li, Ruinan Zhang, Guibin Jiang and Bing Yan
Chemical Society Reviews 2014 - vol. 43(Issue 10) pp:NaN3809-3809
Publication Date(Web):2014/03/19
DOI:10.1039/C3CS60338E
Nanotechnology is having a tremendous impact on our society. However, societal concerns about human safety under nanoparticle exposure may derail the broad application of this promising technology. Nanoparticles may enter the human body via various routes, including respiratory pathways, the digestive tract, skin contact, intravenous injection, and implantation. After absorption, nanoparticles are carried to distal organs by the bloodstream and the lymphatic system. During this process, they interact with biological molecules and perturb physiological systems. Although some ingested or absorbed nanoparticles are eliminated, others remain in the body for a long time. The human body is composed of multiple systems that work together to maintain physiological homeostasis. The unexpected invasion of these systems by nanoparticles disturbs normal cell signaling, impairs cell and organ functions, and may even cause pathological disorders. This review examines the comprehensive health risks of exposure to nanoparticles by discussing how nanoparticles perturb various physiological systems as revealed by animal studies. The potential toxicity of nanoparticles to each physiological system and the implications of disrupting the balance among systems are emphasized.
Co-reporter:Peifu Jiao, Mario Otto, Qiaohong Geng, Chencan Li, Faming Li, Elizabeth R. Butch, Scott E. Snyder, Hongyu Zhou and Bing Yan
Journal of Materials Chemistry A 2016 - vol. 4(Issue 3) pp:NaN520-520
Publication Date(Web):2015/12/07
DOI:10.1039/C5TB02243F
Although nanomaterials have been widely investigated for drug delivery, imaging and immunotherapy, their potential roles in triggering innate cellular immune responses while simultaneously serving as imaging enhancer remain unexplored. In this work, gold nanoparticles (GNPs) conjugated to the tumor-targeting anti-GD2 antibody hu14.18K322A, namely HGNPs, were designed and synthesized to specifically enhance computerized tomography (CT) imaging contrast and to stimulate the attack of neuroblastoma and melanoma cells by natural killer (NK) cells. The HGNPs specifically targeted GD2-positive neuroblastoma (NB1691) and melanoma (M21) cells, with an enhancement of CT contrast images of the HGNP-labeled cell pellets by 5.27- and 7.66-fold, respectively, compared to images of unlabeled cell pellets. The HGNPs also triggered NK-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) in NB1691 and M21 cells with a two-fold higher efficacy compared to that elicited by hu14.18K322A alone, with no adverse effect to GD2-negative PC-3 cells. These results suggest that HGNPs are promising theranostic agents for neuroblastoma and melanoma cancers.