Hua Zuo

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Organization: Southwest University

Department: College of Pharmaceutical Sciences

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Chemicals
References

One DNA strand homo-polymerizes into defined nanostructures

Co-reporter:Mo Li;Jinwen Yu;Xinfeng Zhao;Chengde Mao

Nanoscale (2009-Present) 2017 vol. 9(Issue 30) pp:10601-10605

Publication Date(Web):2017/08/03

DOI:10.1039/C7NR03640J

We report a strategy for programmed DNA self-assembly that is favorable in terms of both thermodynamics and kinetics. In a previous study, it has been demonstrated that DNA self-assembly is primarily driven by thermodynamics and the assembly kinetics is not considered. To reach such stable states at equilibria, prolonged annealing duration is needed. In addition, there are cases where the desired structures could not compete with alternative structures. For example, a single-stranded DNA with a palindromic sequence quickly folds into a one-stranded hairpin instead of forming a two-stranded DNA duplex. Given that most of the DNA tiles are multi-stranded complexes, the kinetic trap represents a challenge to DNA self-assembly. To overcome this problem, we have developed a one-stranded motif that can intramolecularly and quickly fold from a single DNA strand and can be programmed to assemble into a range of nanostructures, including a one-dimensional (1D) ladder, a 1D chain, a two-dimensional (2D) array, and a three-dimensional (3D) triangular prism. All structures have been characterized by polyacrylamide gel electrophoresis (PAGE) and atomic force microscopy (AFM) imaging.

Sensitive detection of strong acidic condition by a novel rhodamine-based fluorescent pH chemosensor

Co-reporter:Jia-Lian Tan;Ting-Ting Yang;Yu Liu;Xue Zhang;Shu-Jin Cheng;Huawei He

Luminescence 2016 Volume 31( Issue 3) pp:865-870

Publication Date(Web):

DOI:10.1002/bio.3043

Abstract

A novel rhodamine-based fluorescent pH probe responding to extremely low pH values has been synthesized and characterized. This probe showed an excellent photophysical response to pH on the basis that the colorless spirocyclic structure under basic conditions opened to a colored and highly fluorescent form under extreme acidity. The quantitative relationship between fluorescence intensity and pH value (1.75–2.62) was consistent with the equilibrium equation pH = pKa + log[(I_max – I)/(I – I_min)]. This sensitive pH probe was also characterized with good reversibility and no interaction with interfering metal ions, and was successfully applied to image Escherichia coli under strong acidity. Copyright © 2015 John Wiley & Sons, Ltd.

A novel sensitive fluorescent turn-on probe for rapid detection of Al3+ and bioimaging

Co-reporter:Shuai Xia, Si-Yu Xiao, Qing-Qing Hong, Jing-Rong Zou, Sen Yang, Mu-Xue Zhang and Hua Zuo

RSC Advances 2015 vol. 5(Issue 7) pp:5244-5249

Publication Date(Web):11 Dec 2014

DOI:10.1039/C4RA14177F

Taking advantage of the condensation reaction of 1-(2-hydroxyphenyl)ethanone and a rhodamine-B derived hydrazide, the first simply-structured rhodamine based probe L for the detection of Al3+ with high efficiency was designed and synthesized which could be applied in water solutions. Spectroscopy showed that the probe responded quickly and selectively to Al3+ over other metal ions with remarkable fluorescence enhancement (43-fold) after 10 equiv. of Al3+ were added. Sensing of Al3+ was proved to be effective over a wide pH range from 4.9 to 8.5 and the limit of the detection was calculated as 1.63 × 10−7 M by the fluorescence titration experiment. Besides, the probe L is characterized with good stability and performed perfectly in a reversibility test with EDTA. Furthermore, live-cell imaging of HeLa cells revealed the cell permeability of the probe, showing no toxicity in cultured cells by MTT method, which indicated that L could be applied in living organisms.

A Case Study of the Likes and Dislikes of DNA and RNA in Self-Assembly

Co-reporter:Hua Zuo;Siyu Wu;Mo Li;Yulin Li;Wen Jiang;Chengde Mao

Angewandte Chemie International Edition 2015 Volume 54( Issue 50) pp:15118-15121

Publication Date(Web):

DOI:10.1002/anie.201507375

Abstract

Programmed self-assembly of nucleic acids (DNA and RNA) is an active research area as it promises a general approach for nanoconstruction. Whereas DNA self-assembly has been extensively studied, RNA self-assembly lags much behind. One strategy to boost RNA self-assembly is to adapt the methods of DNA self-assembly for RNA self-assembly because of the chemical and structural similarities of DNA and RNA. However, these two types of molecules are still significantly different. To enable the rational design of RNA self-assembly, a thorough examination of their likes and dislikes in programmed self-assembly is needed. The current work begins to address this task. It was found that similar, two-stranded motifs of RNA and DNA lead to similar, but clearly different nanostructures.

Inside Cover: A Case Study of the Likes and Dislikes of DNA and RNA in Self-Assembly (Angew. Chem. Int. Ed. 50/2015)

Co-reporter:Hua Zuo;Siyu Wu;Mo Li;Yulin Li;Wen Jiang;Chengde Mao

Angewandte Chemie International Edition 2015 Volume 54( Issue 50) pp:

Publication Date(Web):

DOI:10.1002/anie.201509584

A Case Study of the Likes and Dislikes of DNA and RNA in Self-Assembly

Co-reporter:Hua Zuo;Siyu Wu;Mo Li;Yulin Li;Wen Jiang;Chengde Mao

Angewandte Chemie 2015 Volume 127( Issue 50) pp:15333-15336

Publication Date(Web):

DOI:10.1002/ange.201507375

Abstract

Innentitelbild: A Case Study of the Likes and Dislikes of DNA and RNA in Self-Assembly (Angew. Chem. 50/2015)

Co-reporter:Hua Zuo;Siyu Wu;Mo Li;Yulin Li;Wen Jiang;Chengde Mao

Angewandte Chemie 2015 Volume 127( Issue 50) pp:

Publication Date(Web):

DOI:10.1002/ange.201509584

A novel “off–on” colorimetric and fluorescent rhodamine-based pH chemosensor for extreme acidity

Co-reporter:Jia-Lian Tan, Mu-Xue Zhang, Fang Zhang, Ting-Ting Yang, Yu Liu, Zhu-Bo Li, Hua Zuo

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015 140() pp: 489-494

Publication Date(Web):

DOI:10.1016/j.saa.2014.12.110

The synthesis of 4,7-disubstituted-2H-benzo[b][1,4]-oxazin-3(4H)-ones using Smiles rearrangement and their in vitro evaluation as platelet aggregation inhibitors

Co-reporter:Shuai Xia, Ji-Qiang Liu, Xiu-Hua Wang, Ye Tian, Yu Wang, Jing-Huan Wang, Liang Fang, Hua Zuo

Bioorganic & Medicinal Chemistry Letters 2014 Volume 24(Issue 6) pp:1479-1483

Publication Date(Web):15 March 2014

DOI:10.1016/j.bmcl.2014.02.014

A series of novel benzo[b][1,4]oxazin-3(4H)-one derivatives were synthesized as platelet aggregation inhibitors for structure–activity relationships (SAR) analysis. The synthetic pattern, involved Smiles rearrangement for the preparation of benzoxazine, was proven to be more efficient than the conventional methods. Biological evaluation demonstrated that among all the synthesized compounds, compound 9u (IC50 = 9.20 μM) exhibited the most potent inhibition activity compared with aspirin, the positive control (IC50 = 7.07 μM). Molecular docking revealed that these set of compounds could be the GPIIb/IIIa antagonist for that they could be situated in the binding site of GPIIb/IIIa receptor quite well.

Synthesis of 2H-benzo[b][1,4]oxazin-3(4H)-one derivatives as platelet aggregation inhibitors

Co-reporter:Xiao Tian, Li-Ying Wang, Shuai Xia, Zhu-Bo Li, Xing-Hui Liu, Yuan Yuan, Liang Fang, Hua Zuo

Bioorganic & Medicinal Chemistry Letters 2012 Volume 22(Issue 1) pp:204-206

Publication Date(Web):1 January 2012

DOI:10.1016/j.bmcl.2011.11.027

Novel 2H-benzo[b][1,4]oxazin-3(4H)-ones have been synthesized by condensation, reduction, O-alkylation and Smiles rearrangement using 3-bromo-4-hydroxy benzaldehyde, anilines, and chloroacetyl chloride as starting materials. All the synthesized compounds have been characterized by 1H NMR, 13C NMR, and HRMS, and tested for the inhibitory ability on platelet aggregation. The results have shown that the ADP (adenosine 5′-diphosphate)-induced platelet aggregation was inhibited by 7a–g with the IC50 value at 10.14–18.83 μmol/L. Compound 7a exhibited the most potent inhibitory effect (IC50 = 10.14 μmol/L) among all the compounds, but less potent than the control drug ticlopidine (3.18 μmol/L) and aspirin (6.07 μmol/L). The preliminary structure–activity relationship (SAR) was initially investigated in the study.7a: Platelet aggregation inhibition: IC50 = 10.14 μM.

Synthesis of benzo[b][1,4]oxazin-3(4H)-ones via smiles rearrangement for antimicrobial activity

Co-reporter:Liang Fang;Zhu-Bo Li;Xiao-Yan He;Li-Ying Wang

Medicinal Chemistry Research 2011 Volume 20( Issue 6) pp:670-677

Publication Date(Web):2011 July

DOI:10.1007/s00044-010-9360-z

The benzo[b][1,4]oxazin-3(4H)-one derivatives, 1a–p, carrying F, Br, and Cl on the benzene ring, or benzyl, cyclohexyl, n-hexyl, and tetrafuryl methylene groups attached to nitrogen atom were synthesized via Smiles rearrangement and assayed in vitro for their antimicrobial activity against Gram-positive, Gram-negative bacteria, and fungi. The antimicrobial activity of the benzo[b][1,4]oxazin-3(4H)-ones showed, on the whole, potency toward all the tested Gram-positive and Gram-negative microorganism (MIC ranging from 16 to 64 μg/ml), whereas weak effectiveness was exhibited against fungi. Data obtained suggest that fluorine atom in the compounds, 1c, 1f, 1i plays an important role in enhancing the antimicrobial properties of this class of compounds. These observations provide some predictions to design further antimicrobial active compounds prior to their synthesis according to molecular modeling studies.