⿢Polar constituents of L. chuanxiong were investigated.⿢Three new phthalide glycosides were isolated from the rhizomes of L. chuanxiong..⿢Celephthalide A and icariside F2 were reported from L. chuanxiong for the first time.Three new phthalide glycosides, named chuanxiongoside A (1), chuanxiongoside B (2) and ligusticoside B (3) together with two known glycosides, celephthalide A (4) and icariside F2 (5), were isolated from the rhizomes of Ligusticum chuanxiong. This is the first report of compounds 4⿿5 from the title plant. The structures of compounds 1⿿3 were identified using various spectroscopic methods.

•Four new minor steroidal glycosides were isolated from Helleborus thibetanus.•Structures were determined by 2D NMR, HRMS and hydrolysis.•Steroidal glycosides with double bond at C-25 and glycosidation at 1-OH are unusual.Four new minor spirostanol glycosides (1–4) were isolated from the dried roots and rhizomes of Helleborus thibetanus. Structures of the compounds were established by means of a combination of 1D and 2D NMR experiments, together with HRESIMS and IR measurements as well as the results of acid hydrolysis. The spirostanol glycosides with both a double bond at C-25 and glycosidation at 1-OH have seldom been reported.

•3 bergenin glycosides and 20 known compounds were isolated from R. aesculifolia.•Structures were determined by 2D NMR, IR and HRMS.•This is the first report of bergenin glycosides from nature.Phytochemical investigation on Rodgersia aesculifolia afforded twenty-three compounds, including three bergenin glycosides, previously unknown in nature, together with twenty known compounds. Their structures were elucidated by the extensive use of 1D and 2D NMR experiments, along with IR and HRESIMS spectra. This is the first report of bergenin glycosides from nature.Structures of three bergenin glycosides (1–3) together with twenty known compounds from Rodgersia aesculifolia were elucidated on the basis of spectroscopic analysis.