Yan Wang

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Name: 王彦; Yan Wang

Organization: Shanghai Jiaotong University

Department: School of Pharmacy

Title: Associate Professor

TOPICS

Polymer

UV Absorbents

Chemicals
References

Polyethyleneimine-grafted boronate affinity materials for selective enrichment of cis-diol-containing compounds

Co-reporter:Yun Xue, Wenjun Shi, Bangjie Zhu, Xue Gu, Yan Wang, Chao Yan

Talanta 2015 Volume 140() pp:1-9

Publication Date(Web):1 August 2015

DOI:10.1016/j.talanta.2015.03.008

•A PEI-grafted boronate affinity material was synthesized.•The binding pH was found to be as low as pH 4.5.•Different binding pH can be attributed to the strong electrostatic repulsion.•A separation system was developed based on boronate affinity chromatography, C18-RP chromatography and pCEC.•SiO2@PEI–AAPBA-based SPE technology was applied to the purification of ribonucleosides.Polyethyleneimine (PEI)-grafted and 3-acrylamidophenylboronic acid (AAPBA)-functionalized SiO2 boronate affinity materials were synthesized for the selective enrichment of cis-diol-containing compounds. Characterization results of scanning electron microscopy, Fourier transform infrared spectroscopy, elemental analysis, zeta potential, and X-ray photoelectron spectroscopy indicated the successful fabrication of SiO2@PEI–AAPBA materials. Chromatographic separation of test mixtures reveals that SiO2@PEI–AAPBA has high selective enrichment ability for cis-diol-containing compounds. The binding pH between SiO2@PEI–AAPBA and catechol was found to be as low as pH 4.5, while that between SiO2@PEI–AAPBA and adenosine was only ~7.5. This difference might be attributed to the strong electrostatic repulsion between the solid phase and analytes at a low pH. Furthermore, a diphasic separation column was fabricated based on boronate affinity chromatography, C18-reversed-phase chromatography and applied in pressurized capillary electrochromatography (pCEC). Results showed that four polar nucleosides could be well captured by the boronate affinity chromatography (BAC) section and separated by reversed phase pCEC. Finally, SiO2@PEI600–AAPBA-based solid-phase extraction technology was applied to the purification of ribonucleosides in real urine samples, and results of UHPLC–MS/MS revealed that the intensities of the extracted ions (a neutral mass loss of m/z 132.04 Da) of the ribonucleosides were significantly enhanced after the enrichment.

Metabolic transformation of breast cancer in a MCF-7 xenograft mouse model and inhibitory effect of volatile oil from Saussurea lappa Decne treatment

Co-reporter:Zhang-xiao Peng;Xue Gu;Yun Xue;Qian Wu;Jun-yi Zhou;Chao Yan

Metabolomics 2015 Volume 11( Issue 3) pp:636-656

Publication Date(Web):2015 June

DOI:10.1007/s11306-014-0725-z

Breast cancer results from multi-step carcinogenesis, and the transforming process from normal to malignant cells is associated with profound metabolic disturbances. The metabolic transformation due to interplay between tumor and host during the development of breast cancer and its implications for breast cancer therapy have not been extensively investigated. Here, we report a metabonomic study on MCF-7 xenograft mice to delineate characteristic metabolic transformation during the development of breast cancer using a comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC–TOF/MS) and an ultra performance liquid chromatography–quadrupole time-of-flight mass spectrometry. Volatile oil extracted from Saussurea lappa Decne (VOSL), and Costunolide and Dehydrocostus lactone (Cos–Dehy), isolated from VOSL, were used to treat the MCF-7 xenograft mice to evaluate their efficiency and to investigate their pharmacological mechanism on inhibiting the growth of tumor. The dynamic changes of serum and urine metabolic profiles indicated that both VOSL and Cos–Dehy were able to attenuate metabolic perturbation MCF-7 xenograft mice, which is consistent with their efficiency that VOSL and Cos–Dehy significantly inhibit the growth of MCF-7 xenograft tumor. Significant alterations of key metabolic pathways, including elevated glycolysis and steroid hormone metabolism, and decreased unsaturated fatty acids metabolism, were observed in MCF-7 xenograft mice, which were attenuated or normalized by VOSL and Cos–Dehy treatment. Arachidonate, docosahexaenoic acid, eicosapentaenoic acid, linoleate, dihomo-γ-linolenate, 20α-hydroxyprogesterone, and cortisone were selected as a panel of candidate pathological biomarkers of MCF-7 xenografts, which may be further developed to be valuable diagnostic markers for the early detection of breast cancer.

Solid-phase extraction approach for phospholipids profiling by titania-coated silica microspheres prior to reversed-phase liquid chromatography–evaporative light scattering detection and tandem mass spectrometry analysis

Co-reporter:Juan Bian, Yun Xue, Kai Yao, Xue Gu, Chao Yan, Yan Wang

Talanta 2014 Volume 123() pp:233-240

Publication Date(Web):June 2014

DOI:10.1016/j.talanta.2014.02.001

•Titania-coated silica microspheres (TiO2/SiO2) were synthesized and characterized.•TiO2/SiO2 microspheres were used as solid-phase extraction (SPE) sorbent.•The SPE strategy for selective capture of phospholipids (PLs) was developed by RPLC–ELSD.•This SPE approach was applied in serum PLs profiling prior to UPLC–MS analysis.•This SPE strategy used in PLs screening has high selectivity and efficiency.A novel strategy for selectively adsorbing phospholipids (PLs) on titania-coated silica core–shell microspheres (TiO2/SiO2) was developed. The TiO2/SiO2 microspheres were prepared through water-vapor-induced internal hydrolysis and then characterized by SEM, UV–vis spectroscopy, X-ray diffraction, and measurements of Brunauer–Emmett–Teller surface area. Analyses showed that the titania layer was uniformly distributed onto the surface of silica particles. The TiO2/SiO2 microspheres were employed as sorbent in solid-phase extraction (SPE), and their absorptive ability was investigated by reversed-phase liquid chromatography–evaporative light scattering detection (RPLC–ELSD). Important factors that affect the extraction, such as loading buffer, eluting buffer, and elution volume, were investigated in detail and optimized by using standard samples. Results reveal that the developed SPE approach had higher recoveries for PLs than that based on pure TiO2 particles. The proposed SPE method was used for extraction of PLs from serum and showed great potential for identifying more kinds of endogenous PL metabolites by ultra performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC–QTOF MS). The proposed SPE method with the composite sorbent was used to screen PLs from a biological matrix with high selectivity and efficiency. This approach is a promising method for selective extraction of PLs in lipidomics or phospholipidomics.

Two-dimensional strong cation-exchange liquid chromatography/reversed-phase pressurized capillary electrochromatography for separation of complex samples

Co-reporter:Yi Wu;Xue Gu;Lin Zhang;Chao Yan

Journal of Separation Science 2011 Volume 34( Issue 9) pp:1027-1034

Publication Date(Web):

DOI:10.1002/jssc.201000815

Abstract

A 2-D separation platform was constructed using micro strong cation-exchange liquid chromatography (μ-SCXLC) and reversed-phase pressurized capillary electrochromatography (RP-pCEC) for the analysis of complex samples. Samples were fractionated by the first-dimension μ-SCXLC with a linear solvent gradient and then injected into the second-dimension RP-pCEC for further separation. The μ-SCXLC/RP-pCEC 2-D system with three separation mechanisms, namely strong cation-exchange, reversed-phase chromatography and electrophoresis, provided high selectivity, high resolution and high peak capacity compared to one-dimensional chromatographic approaches. Separation effectiveness of this 2-D system was demonstrated by the analysis of different kinds of complex samples, such as traditional Chinese medicine Cortex Phellodendri, bovine serum albumin (BSA) tryptic digest and real serum tryptic digest. A theoretical peak capacity of approximately 1200 was achieved, which proves its promising potential for the separation and analysis of complex samples.

HPLC-ELSD analysis of spectinomycin dihydrochloride and its impurities

Co-reporter:Junyi Zhou;Lin Zhang;Chao Yan

Journal of Separation Science 2011 Volume 34( Issue 15) pp:1811-1819

Publication Date(Web):

DOI:10.1002/jssc.201100065

Abstract

A simple, rapid and reliable reversed-phase ion-pair chromatography method by HPLC coupled to an evaporative light scattering detector (ELSD) has been developed to simultaneously determine chloride, spectinomycin and its related substances in a sample. The column was a TSKgel ODS-100V. The mobile phase was ACN/aqueous solution of 15 mM ammonium acetate adjusted with TFA to pH 3.0 (2:98 v/v), in an isocratic mode. The drift tube temperature was set at 50°C and the nebulizing gas flow rate of air was 3.5 L/min for ELSD detection. Almost all of the reported degradation compounds of spectinomycin such as actinamine, actinospectinoic acid and biosynthesis intermediates such as dihydrospectinomycin diastereoisomers were baseline separated. MS was utilized for the identification of spectinomycin and its seven related substances. The method for the assay of spectinomycin was successfully validated with respect to accuracy, precision (RSD less than 2%), linearity (throughout the linear range 0.025–3 mg/mL, r=0.9993), sensitivity (LOD: 100 ng on column) and robustness. The experimental results demonstrated that the simultaneous determination of chloride, spectinomycin and related substances is feasible in a single run, which suggests applicability in routine assays.