Yanfei Tan

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Organization: Sichuan University

Department: National Engineering Research Center for Biomaterials

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TOPICS

Analytical Chemistry

Fluorescent Probes

Chemicals
References

A novel coumarin derivative as a sensitive probe for tracing intracellular pH changes

Co-reporter:Mengqiang Liu, Minshan Hu, Qian Jiang, Zhiyun Lu, Yan Huang, Yanfei Tan and Qing Jiang

RSC Advances 2015 vol. 5(Issue 21) pp:15778-15783

Publication Date(Web):19 Jan 2015

DOI:10.1039/C4RA11347K

A new pH sensitive probe, 7-diethylamino-3-[2-(4-piperidin-1-yl-phenyl)-vinyl]-chromen-2-one (CS-P), was designed and synthesized. This probe was constructed by introduction of piperidine to the coumarin stem. It exhibited high pH sensitivity ranging from pH 5.9 to pH 3.0 (pKa = 4.55), and in vitro cell imaging showed that its fluorescence intensity was distinctly related to the cellular pH changes induced by chloroquine or dexamethasone. Demonstrated as having good cell membrane permeability and high pH sensitivity, this probe is suitable for tracing the intracellular pH changes from neutral to acidic conditions.

Bone morphogenetic protein Smads signaling in mesenchymal stem cells affected by osteoinductive calcium phosphate ceramics

Co-reporter:Zhurong Tang;Zhe Wang;Fangzhu Qing;Yilu Ni;Yujiang Fan;Xingdong Zhang

Journal of Biomedical Materials Research Part A 2015 Volume 103( Issue 3) pp:1001-1010

Publication Date(Web):

DOI:10.1002/jbm.a.35242

Abstract

Porous calcium phosphate ceramics (CaP ceramics) could induce ectopic bone formation which was regulated by various signal molecules. In this work, bone marrow mesenchymal stem cells (MSCs) were cultured on the surface of osteoinductive hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramics in comparison with control (culture plate) for up to 14 days to detect the signal molecules which might be affected by the CaP ceramics. Without adding osteogenic factors, MSCs cultured on HA and BCP both expressed higher Runx2, Osterix, collagen type I, osteopontin, bone sialoprotein, and osteocalcin at various stages compared with control, thus confirmed the osteoblastic differentiation of MSCs. Later study demonstrated the messenger RNA level of bone morphogenetic protein 2 (BMP2) and BMP4 were also significantly enhanced by HA and BCP. Furthermore, Smad1, 4, 5, and Dlx5, the main molecules in the BMP/Smads signaling pathway, were upregulated by HA and BCP. Moreover, the higher expression of Smads and BMP2, 4 in BCP over HA, corresponded to the better performance of BCP in stimulating in vitro osteoblastic differentiation of MSCs. This was in accordance with the better osteoinductivity of BCP over HA in vivo. Altogether, these results implied that the CaP ceramics may initiate the osteoblastic differentiation of MSCs by influencing the expression of molecules in BMP/Smads pathway. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1001–1010, 2015.

A coumarin-based fluorescent turn-on probe for detection of biothiols in vitro

Co-reporter:Mengqiang Liu;Qian Jiang;Zhiyun Lu;Yan Huang;Qing Jiang

Luminescence 2015 Volume 30( Issue 8) pp:1395-1402

Publication Date(Web):

DOI:10.1002/bio.2912

Abstract

A novel fluorescent probe (CA-N) was designed and synthesized for detection of biothiols. CA-N displayed a strong fluorescence in the presence of biothiols with high sensitivity, and the mechanism for detection biothiols was based on the Michael addition reaction of a thiol group to α,β-unsaturated ketones. CA-N showed low detection limit for cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), which were calculated as 3.16, 0.19 and 5.15 μM, respectively. At the same time, CA-N exhibited high selectivity toward biothiols compared with other biological amino acids. In vitro cell experiments proved that CA-N had no cytotoxicity, high cell permeability and could be employed in living cell imaging for biothiols. Copyright © 2015 John Wiley & Sons, Ltd.

A novel nitro-substituted benzothiadiazole as fluorescent probe for tumor cells under hypoxic condition

Co-reporter:Qian Jiang, Zhanyuan Zhang, Jiao Lu, Yan Huang, Zhiyun Lu, Yanfei Tan, Qing Jiang

Bioorganic & Medicinal Chemistry 2013 Volume 21(Issue 24) pp:7735-7741

Publication Date(Web):15 December 2013

DOI:10.1016/j.bmc.2013.10.019

Most of solid tumor cells are hypoxic and hard to trace and measure. A new compound, 4,7-bis(4-dodecylthiophen-2-yl)-5,6-dinitrobenzo[c][1,2,5]thiadiazole (BTTD-NO2), was synthesized for labeling the hypoxic cells specially in this paper. BTTD-NO2 showed no cytotoxicity to MG63 cells by MTT method. When MG63 cells were cultured with BTTD-NO2 under hypoxic condition for 24 h, strong red fluorescence distribution in cytoplasm was observed. Flow cytometry results showed that 65% of MG63 cells were labeled with strong red fluorescence in hypoxic condition while only 2.4% in oxic condition. Furthermore, Real time RT-PCR proved that BTTD-NO2 could stimulate high gene expression of the nitroreductase in the cells which could improve the conversion rate of BTTD-NO2 to BTTD-NH2 in turn. It proved that the fluorescence of BTTD-NO2 was quenched by its two nitro groups, however, strong red fluorescence could emit in the cytoplasm after the reduction of its nitro groups to amino groups in the tumor cells under hypoxic condition. These results suggested that BTTD-NO2 had the potential as a superior fluorescent probe for tumor detection.A new compound, 4,7-bis(4-dodecylthiophen-2-yl)-5,6-dinitrobenzo[c][1,2,5]thiadiazole (BTTD-NO2), was synthesized in this paper. The fluorescence of BTTD-NO2 was quenched by its two nitro groups. However, the tumors cells were stained with strong red fluorescence after reduction of BTTD-NO2 via transformation of its nitro groups to amino groups under hypoxic condition. The results suggested that BTTD-NO2 had the potential as a superior fluorescent probe for labeling the hypoxic tumor cells specially.