N.P. Lockyer - Cochemist

Chemicals
References

Imaging of metabolites using secondary ion mass spectrometry

Co-reporter:Emily G. Armitage;Helen L. Kotze;Nicholas P. Lockyer

Metabolomics 2013 Volume 9( Issue 1 Supplement) pp:102-109

Publication Date(Web):2013 March

DOI:10.1007/s11306-012-0477-6

This article provides an overview of the technique of secondary ion mass spectrometry imaging and highlights some current and future areas of application relevant to the field of metabolomics. The approach benefits from label-free analysis of molecular species up to ~1500 Da with minimal sample preparation. Offering the highest spatial resolution of current mass spectrometry imaging methodologies, the technique is well-suited to metabolite imaging in both biological tissue and cells, in both 2D and 3D.

ToF-SIMS studies of Bacillus using multivariate analysis with possible identification and taxonomic applications

Co-reporter:C.E. Thompson, J. Ellis, J.S. Fletcher, R. Goodacre, A. Henderson, N.P. Lockyer, J.C. Vickerman

Applied Surface Science 2006 Volume 252(Issue 19) pp:6719-6722

Publication Date(Web):30 July 2006

DOI:10.1016/j.apsusc.2006.02.237

Abstract

In this paper we discuss the application of ToF-SIMS with an Au₃⁺ primary ion beam, combined with principal components analysis (PCA) and discriminant function analysis (DFA) for the identification of individual strains of two Bacillus species. The ToF-SIMS PC-DFA methodology is capable of distinguishing bacteria at the strain level based on analysis of surface chemical species. By classifying the data using hierarchical cluster analysis (HCA) we are able to show quantitative separation of species and of these strains. This has taxonomic implications in the areas of rapid identification of pathogenic microbes isolated from the clinic, food and environment.