Co-reporter:Yiping Chen, Yunlei Xianyu, Jing Wu, Mingling Dong, Wenshu Zheng, Jiashu Sun, and Xingyu Jiang
Analytical Chemistry May 16, 2017 Volume 89(Issue 10) pp:5422-5422
Publication Date(Web):April 19, 2017
DOI:10.1021/acs.analchem.7b00239
We report an ultrasensitive, quantitative, and rapid bioluminescent immunosensor (ABS) for point-of-care testing (POCT) of the disease biomarker in clinical samples using double enzymes including alkaline phosphatase (ALP) and luciferase. In the presence of the biomarker, the ALP attached on the surface of immuno-nanocomplex dephosphorylates adenine triphosphate (ATP), subsequently inhibiting the ATP–luciferin–luciferase bioluminescent reaction. The highly sensitive response of ATP (picomolar level) allows for ultrasensitive detection of biomarker via the effective change of the bioluminescence intensity through ALP- and luciferase-catalyzed reactions, which can be quantitatively determined by a portable ATP detector. This ABS fulfills the criteria for POCT that performs sensitive (femtomolar level of biomarkers) and quantitative measurement quickly (less than 1 h) with minimal equipment (portable detector).
Co-reporter:Bei Ran, Yunlei Xianyu, Mingling Dong, Yiping Chen, Zhiyong Qian, and Xingyu Jiang
Analytical Chemistry June 6, 2017 Volume 89(Issue 11) pp:6113-6113
Publication Date(Web):May 1, 2017
DOI:10.1021/acs.analchem.7b00831
This work demonstrates a highly sensitive peroxide test strip (PTS)-based enzyme-linked immunosorbent assay (ELISA) for both qualitative and quantitative detection of drugs of abuse (morphine) and disease biomarkers (interleukin-6 and HIV-1 capsid antigen p24). This color-based PTS is a commercially available product with advantages of low cost, easy operation, and portability, and it is an ideal signal readout strategy in ELISA to simplify the immunoassay procedures and enable point-of-care testing (POCT). In addition, we introduce the bioorthogonal reaction that can effectively amplify the signal by controlling the cycles of bioorthogonal reaction to achieve the desirable sensitivity depending on different analytes. The limit of detection is 0.2 ng/mL for morphine, 3.98 pg/mL for interleukin-6, and 11.6 pg/mL for detection of HIV-capsid antigen (p24). This PTS-ELISA applies to both the qualitative and quantitative detection of IL-6 and p24 in clinical serum samples with good accuracy, which provides a promising tool for the POCT in clinical diagnosis.
Co-reporter:Wenshu Zheng, Yuexiao Jia, Wenwen Chen, Guanlin Wang, Xuefeng Guo, and Xingyu Jiang
ACS Applied Materials & Interfaces June 28, 2017 Volume 9(Issue 25) pp:21181-21181
Publication Date(Web):June 5, 2017
DOI:10.1021/acsami.7b05230
We provide a facile and scalable strategy for preparing gold nanoparticles (AuNPs)-based antibacterial coating on a variety of surfaces through electrostatic self-assembly. AuNPs conjugated with 4,6-diamino-2-pyrimidinethiol (DAPT, not antibacterial by itself), AuDAPT, can form stable coating on different substrates made from polyethylene (PS), polyvinyl chloride (PVC), polypropylene (PP), polyethylene (PE), polydimethylsiloxane (PDMS), and SiO2 in one step. Such a coating can efficiently eradicate pathogenic Gram-negative bacteria and even multidrug-resistant (MDR) mutants without causing any side-effect such as cytotoxicity, hemolysis, coagulation, and inflammation. We show that immobilized AuDAPT, instead of AuDAPT released from the substrate, is responsible for killing the bacteria and that the antimicrobial components do not enter into the environment to cause secondary contamination to breed drug resistance. Advantages for such coating include applicability on a broad range of surfaces, low cost, stability, high antibacterial efficiency, good biocompatibility, and low risk in antibiotics pollution; these advantages may be particularly helpful in preventing infections that involve medical devices.Keywords: antimicrobial; biocompatibility; gold nanoparticles; nosocomial infections; self-assembly;
Co-reporter:Yiping Chen, Yunlei Xianyu, and Xingyu Jiang
Accounts of Chemical Research 2017 Volume 50(Issue 2) pp:
Publication Date(Web):January 9, 2017
DOI:10.1021/acs.accounts.6b00506
ConspectusAs one of the major tools for and by chemical science, biochemical analysis is becoming increasingly important in fields like clinical diagnosis, food safety, environmental monitoring, and the development of chemistry and biochemistry. The advancement of nanotechnology boosts the development of analytical chemistry, particularly the nanoparticle (NP)-based approaches for biochemical assays. Functional NPs can greatly improve the performance of biochemical analysis because they can accelerate signal transduction, enhance the signal intensity, and enable convenient signal readout due to their unique physical and chemical properties. Surface chemistry is a widely used tool to functionalize NPs, and the strategy for surface modification is of great significance to the application of NP-mediated biochemical assays. Surface chemistry not only affects the quality of NPs (stability, monodispersity, and biocompatibility) but also provides functional groups (−COO–, −NH3+, −CHO, and so on) or charges that can be exploited for bioconjugation or ligand exchange. Surface chemistry also dictates the sensitivity and specificity of the NP-mediated biochemical assays, since it is vital to the orientation, accessibility, and bioactivity of the functionalized ligands on the NPs.In this Account, we will focus on surface chemistry for functionalization of gold nanoparticles (AuNPs) with small organic molecules for biochemical analysis. Compared to other NPs, AuNPs have many merits including controllable synthesis, easy surface modification and high molar absorption coefficient, making them ideal probes for biochemical assays. Small-molecule functionalized AuNPs are widely employed to develop sensors for biochemical analysis, considering that small molecules, such as amino acids and sulfhydryl compounds, are more easily and controllably bioconjugated to the surface of AuNPs than biomacromolecules due to their less complex structure and steric hindrance. The orientation and accessibility of small molecules on AuNPs in most cases can be precisely controlled without compromising their bioactivity as well, thus ensuring the performance, such as the specificity and sensitivity, of AuNP-based biochemical assays.This Account reviews recent progress in the surface chemistry of functionalized AuNPs for biochemical assays. The surface chemistries mainly include click chemistry, ligand exchange reaction, and coordination-based recognition. These surface-modified AuNPs allow for assaying a range of important biochemical markers including metal ions, small biomolecules, enzymes, and antigens and antibodies. Applications of these systems range from environmental monitoring to medical diagnostics. This Account highlights the advantages and limitations (sensitivity, detection efficiency, and stability) that AuNP-mediated assays still have compared with conventional analytical methods. This Account also discusses the future research directions of surface-modified AuNP-mediated biochemical analysis. The main aim of this Account is to summarize the current surface modification strategies for AuNPs and further demonstrate how to make use of surface modification strategies to effectively improve the performance of AuNP-mediated analytical methods for a wide variety of applications relating to biochemical analysis.
Co-reporter:Binfeng Hu;Juanjuan Li;Lei Mou;Yong Liu;Jinqi Deng;Wei Qian;Jiashu Sun;Ruitao Cha
Lab on a Chip (2001-Present) 2017 vol. 17(Issue 13) pp:2225-2234
Publication Date(Web):2017/06/27
DOI:10.1039/C7LC00249A
Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.
Co-reporter:Yuexiao Jia;Yongming Guo;Shiwen Wang;Wenwen Chen;Jiangjiang Zhang;Wenshu Zheng
Nanoscale (2009-Present) 2017 vol. 9(Issue 28) pp:9811-9817
Publication Date(Web):2017/07/20
DOI:10.1039/C7NR01775H
We describe an ultra-stable, ultra-robust, straightforward and low-cost approach for the colorimetric detection of H2S with nanocrystalline cellulose (NCC) based on the reaction of H2S with lead acetate. The presence of NCC not only mediates the seed growth of a PbS/NCC complex, but also acts as a stabilizer protecting PbS from precipitation. This stable system is so robust that it can be used to quantitatively detect H2S even after two-year storage.
Co-reporter:Lingmin Zhang, Xinglong Yang, Ying Li, Wenfu Zheng, Xingyu Jiang
Carbon 2017 Volume 121(Volume 121) pp:
Publication Date(Web):1 September 2017
DOI:10.1016/j.carbon.2017.05.084
The synergistic treatment with therapeutic nucleic acids and chemotherapeutics is considered to be a feasible strategy to overcome drug-resistant cancers. Herein, we constructed a novel amine dotted hollow carbon nanospheres (HCNs) to serve as a versatile platform for co-delivery of siRNA targeting multidrug resistance gene (MDR1) mRNA (siMDR1) and chemotherapeutics (Doxorubicin or Cisplatin) to fight drug-resistant cancers. The HCNs show enhanced loading capability of both siRNA and chemotherapeutics. The nanostructure down-regulates more than ∼96% of MDR1 protein expression of DOX-resistant breast cancer (MCF-7/ADR cells) and leads to ∼90% reduction of weight of MCF-7/ADR tumour on mice. Thus, the HCNs can be used as a good platform for drug delivery in cancer therapy.We construct a novel amine-coated hollow carbon nanospheres (HCNs), which serve as a versatile platform for co-delivery of siRNA and chemotherapeutics (DOX or Cis) to fight drug-resistant cancers. The nanostructures exhibit enhanced nucleic acid and chemotherapeutics delivery, effective gene regulation, and synergistically inhibit drug-resistant cancers in vitro and in vivo.Download high-res image (327KB)Download full-size image
Co-reporter:Jing Wu, Yiping Chen, Mingzhu Yang, Yu Wang, Cheng Zhang, Mo Yang, Jiashu Sun, Mengxia Xie, Xingyu Jiang
Analytica Chimica Acta 2017 Volume 982(Volume 982) pp:
Publication Date(Web):22 August 2017
DOI:10.1016/j.aca.2017.05.031
•The SA-B-HRP nanocomplex is an effective signal amplification system in MIS.•MIS has been developed for detection of PCT and IL-6 simultaneously.•The LOD for PCT and IL-6 by MIS are 48.9 pg mL−1 and 1.0 pg mL−1, respectively.•MIS has successfully detected PCT and IL-6 simultaneously in serum samples.Simultaneous, sensitive and quantitative detection of biomarkers in infectious disease is crucial for guiding antimicrobial treatment and predicting prognosis. This work reported an ultrasensitive and quantitative microfluidic immunoassay combined with the streptavidin-biotin-peroxidase (SA-B-HRP) nanocomplex-signal amplification system (MIS) to detect two inflammatory biomarkers, procalcitonin (PCT, for discriminating bacterial infections from nonbacterial infections) and interleukin-6 (IL-6, for monitoring the kinetics of infectious disease) simultaneously. The amplification system was based on the one step self-assembly of SA and B-HRP to form the SA-B-HRP nanocomplex, which effectively amplified the chemiluminescent signals. The linear ranges for PCT and IL-6 detections by MIS were 250–1.28 × 105 pg mL−1 and 5–1280 pg mL−1, and the limit of detection (LOD) were 48.9 pg mL−1 and 1.0 pg mL−1, respectively, both of which were significantly improved compared with microfluidic immunoassays without amplification system (MI). More importantly, PCT and IL-6 in human serum could be simultaneously detected in the same run by MIS, which could greatly improve the detection efficiency and reduce the cost. Given the advantages of high sensitivity, multiplex and quantitative detection, MIS could be potentially applied for detection of biomarkers at low concentration in clinical diagnosis.Download high-res image (267KB)Download full-size image
Co-reporter:Shiyu Cheng;Yu Jin;Nuoxin Wang;Feng Cao;Wei Zhang;Wei Bai;Wenfu Zheng
Advanced Materials 2017 Volume 29(Issue 28) pp:
Publication Date(Web):2017/07/01
DOI:10.1002/adma.201700171
A self-adjusting, blood vessel-mimicking, multilayered tubular structure with two polymers, poly(ε-caprolactone) (PCL) and poly(dl-lactide-co-glycolide) (PLGA), can keep the shape of the scaffold during biodegradation. The inner (PCL) layer of the tube can expand whereas the outer (PLGA) layers will shrink to maintain the stability of the shape and the inner space of the tubular shape both in vitro and in vivo over months. This approach can be generally useful for making scaffolds that require the maintenance of a defined shape, based on FDA-approved materials.
Co-reporter:Lei Mou
Advanced Healthcare Materials 2017 Volume 6(Issue 15) pp:
Publication Date(Web):2017/08/01
DOI:10.1002/adhm.201601403
Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user-friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials-related aspects of the recent advances in microfluidics-based immunoassays for point-of-care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.
Co-reporter:Ying Li;Kai Jiang;Jian Feng;Jinzhe Liu;Rong Huang;Zhaojun Chen;Junchuan Yang;Zhaohe Dai;Yong Chen;Nuoxin Wang;Wenjin Zhang;Wenfu Zheng;Guang Yang
Advanced Healthcare Materials 2017 Volume 6(Issue 11) pp:
Publication Date(Web):2017/06/01
DOI:10.1002/adhm.201601343
Bacterial cellulose (BC) membranes with shape-memory properties allow the rapid preparation of artificial small-diameter blood vessels when combined with microfluidics-based patterning with multiple types of cells. Lyophilization of a wet multilayered rolled BC tube endows it with memory to recover its tubular shape after unrolling. The unrolling of the BC tube yields a flat membrane, and subsequent patterning with endothelial cells, smooth muscle cells, and fibroblast cells is carried out by microfluidics. The cell-laden BC membrane is then rerolled into a multilayered tube. The different cells constituting multiple layers on the tubular wall can imitate blood vessels in vitro. The BC tubes (2 mm) without cell modification, when implanted into the carotid artery of a rabbit, maintain thrombus-free patency 21 d after implantation. This study provides a novel strategy for the rapid construction of multilayered small-diameter BC tubes which may be further developed for potential applications as artificial blood vessels.
Co-reporter:Peng Wang;Lingmin Zhang;Yangzhouyun Xie;Nuoxin Wang;Rongbing Tang;Wenfu Zheng
Advanced Science 2017 Volume 4(Issue 11) pp:
Publication Date(Web):2017/11/01
DOI:10.1002/advs.201700175
AbstractThe type II bacterial clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 (CRISPR-associated protein) system (CRISPR-Cas9) is a powerful toolbox for gene-editing, however, the nonviral delivery of CRISPR-Cas9 to cells or tissues remains a key challenge. This paper reports a strategy to deliver Cas9 protein and single guide RNA (sgRNA) plasmid by a nanocarrier with a core of gold nanoclusters (GNs) and a shell of lipids. By modifying the GNs with HIV-1-transactivator of transcription peptide, the cargo (Cas9/sgRNA) can be delivered into cell nuclei. This strategy is utilized to treat melanoma by designing sgRNA targeting Polo-like kinase-1 (Plk1) of the tumor. The nanoparticle (polyethylene glycol-lipid/GNs/Cas9 protein/sgPlk1 plasmid, LGCP) leads to >70% down-regulation of Plk1 protein expression of A375 cells in vitro. Moreover, the LGCP suppresses melanoma progress by 75% on mice. Thus, this strategy can deliver protein-nucleic acid hybrid agents for gene therapy.
Co-reporter:Lingmin Zhang;Peng Wang;Wenfu Zheng
Journal of Materials Chemistry B 2017 vol. 5(Issue 32) pp:6601-6607
Publication Date(Web):2017/08/16
DOI:10.1039/C7TB01105A
We present novel hollow carbon nanospheres (HCNs) for targeted breast cancer therapy. The HCNs have a 40 nm-cavity for drugs loading, and the surface is covered with “natural” primary amine without any exogenous modification, equipped to conjugate with functional biomolecules. After modification with anti-HER2 antibody and loading of Doxorubicin (DOX) to the HCNs, the HER2@HCNs/DOX can specifically access HER2-positive breast cancer cells and significantly suppress the cells in vitro, causing ∼60% reduction of the volume of the HER2-positive tumours in vivo. The HCNs show great promise to become a multifunctional carrier in the field of high-loading capacity drug delivery for cancer therapy.
Co-reporter:Lingmin Zhang;Peng Wang;Wenfu Zheng
Journal of Materials Chemistry B 2017 vol. 5(Issue 32) pp:6601-6607
Publication Date(Web):2017/08/16
DOI:10.1039/C7TB01105A
We present novel hollow carbon nanospheres (HCNs) for targeted breast cancer therapy. The HCNs have a 40 nm-cavity for drugs loading, and the surface is covered with “natural” primary amine without any exogenous modification, equipped to conjugate with functional biomolecules. After modification with anti-HER2 antibody and loading of Doxorubicin (DOX) to the HCNs, the HER2@HCNs/DOX can specifically access HER2-positive breast cancer cells and significantly suppress the cells in vitro, causing ∼60% reduction of the volume of the HER2-positive tumours in vivo. The HCNs show great promise to become a multifunctional carrier in the field of high-loading capacity drug delivery for cancer therapy.
Co-reporter:Mingzhu Yang;Wei Zhang;Wenshu Zheng;Fengjing Cao
Lab on a Chip (2001-Present) 2017 vol. 17(Issue 22) pp:3874-3882
Publication Date(Web):2017/11/07
DOI:10.1039/C7LC00780A
This study reports a simple, rapid, low-cost, robust, and multiplexed barcoded paper-based assay (BPA) compatible with mobile devices. An inkjet printer and an XYZ dispensing platform were used to realize mass-manufacturing of barcoded paper-based analytical devices (BPADs) with high precision and efficiency. We designed a new group of barcodes and developed an application (APP) for the reading of the new code. The new barcodes possess a 16 times higher coding capacity than the standard Codabar code in our experiment on drug residue detection. The BPA system allows applications in the assays of blood-transmitted infections, drug residues in milk and multiplex nucleic acids. The whole detection process and the readout of the results can be completed within 10 minutes. The limit of detection for enrofloxacin (ENR) (8 ng mL−1) satisfies the requirements of drug residue monitoring. Its high rapidity, simplicity, efficiency and selectivity make the BPA system extremely suitable to be applied in rapid and on-site detection.
Co-reporter:Binfeng Yin;Wenshu Zheng;Mingling Dong;Wenbo Yu;Yiping Chen;Sang Woo Joo
Analyst (1876-Present) 2017 vol. 142(Issue 16) pp:2954-2960
Publication Date(Web):2017/08/07
DOI:10.1039/C7AN00779E
We developed a competitive colorimetric nanosensor based on Au@Ag bimetallic nanoparticles for the detection of interleukin-6 (IL-6) in clinical samples. Antibody-conjugated magnetic nanoparticles (MNPs) and polystyrene (PS) microparticles conjugated with both catalase and a secondary antibody can form sandwich structures that enable one-step target enrichment and separation. The catalase on the surface of the PS can catalyze the hydrolysis of hydrogen peroxide (H2O2) to regulate the deposition of Ag+ on the surface of gold nanoparticles (AuNPs), and forms different sizes and amounts of Au@Ag bimetallic nanoparticles (Au@AgNPs) which produce a distinct color signal for readout with the naked eye. Our sensor features high sensitivity, selectivity, reproducibility and anti-interference property as a result of comprehensive parameter optimization. The limit of detection of IL-6 can reach 11 pg mL−1 with the naked eye and 1.2 pg mL−1 by quantitative instrumental analysis. The whole analysis can be finished within 1 h. More importantly, we successfully apply our platform or the detection of IL-6 in clinical samples with better accuracy than conventional enzyme-linked immunosorbent assay (ELISA).
Co-reporter:Hongyan Sun;Ye Liu;Cuntong Zhang;Xuegang Luo
Analytical Methods (2009-Present) 2017 vol. 9(Issue 7) pp:1185-1189
Publication Date(Web):2017/02/16
DOI:10.1039/C6AY03489F
At the early stage of HIV infection, the low concentration of HIV components seriously hinders the diagnosis of this fatal disease. Improving the sensitivity of detection thus becomes the only approach to effectively detect HIV infection at the early stages. We use alkaline phosphatase to catalyze short peptides that contain a 2-phenylalanine core component (2F) to form β-sheets which can combine with Congo red to produce a strong fluorescent signal. For the detection of HIV-1 p24, the limit of detection of 2F-Congo red reaches 2.44 pg ml−1, which is lower than that of clinical ELISA (5 pg ml−1). The inter-batch variability, intra-batch variability and precision of 2F-Congo red for HIV detection are 5.4%, 2.3% and 2.9%, respectively, which are similar to those of clinical ELISA (4.1%, 2.7% and 3.2%, respectively). The variability and precision of 2F-Congo red is consistent to that of clinical ELISA for quantifying HIV-1 p24. 2F-Congo red thus shows a better performance for the detection of HIV infection at the early stages, as detection by clinical ELISA is difficult at the early stages of infection.
Co-reporter:Junchuan Yang;Xinglong Yang;Le Wang;Wei Zhang;Wenbo Yu;Nuoxin Wang;Baogan Peng;Wenfu Zheng;Guang Yang
Nanoscale (2009-Present) 2017 vol. 9(Issue 35) pp:13095-13103
Publication Date(Web):2017/09/14
DOI:10.1039/C7NR03944A
We present a total tissue engineered (TE) intervertebral disc (IVD) to address IVD degradation, which is a major cause of chronic neck and back pain. The TE IVD is comprised of an alginate hydrogel-based nucleus pulposus (NP) and hierarchically organized, concentric ring-aligned electrospun (ES) polycaprolactone (PCL)/poly (D,L-lactide-co-glycolide) (PLGA)/Collagen type I (PPC)-based annulus fibrosus (AF). The TE IVD exhibits excellent hydrophilicity to simulate highly hydrated native IVD. Long-term in vivo implantation assays demonstrate the excellent structural (shape maintenance, hydration, and integration with surrounding tissues) and functional (mechanical supporting and flexibility) performances of the TE IVD. Our study provides a novel approach for treating IVD degeneration.
Co-reporter:Xinglong Yang;Nuoxin Wang;Lingmin Zhang;Luru Dai;Huawu Shao
Nanoscale (2009-Present) 2017 vol. 9(Issue 14) pp:4770-4776
Publication Date(Web):2017/04/06
DOI:10.1039/C7NR00342K
Multi-photon excitation and versatile fluorescent probes are in high need for biological imaging, since one probe can satisfy many needs as a biosensor. Herein we synthesize a series of two-photon excited probes based on tetraphenylethene (TPE) structures (TPE-Acr, TPE-Py, and TPE-Quino), which can image both mammalian cells and bacteria based on aggregation-induced emission (AIE) without washing them. Because of cationic moieties, the fluorescent molecules can aggregate into nanoscale fluorescent organic nanoscale dots to image mitochondria and bacteria with tunable emissions using both one-photon and two-photon excitation. Our research demonstrates that these AIE-dots expand the functions of luminescent organic dots to construct efficient fluorescent sensors applicable to both one-photon and two-photon excitation for bio-imaging of bacteria and mammalian cells.
Co-reporter:Yong Liu
Lab on a Chip (2001-Present) 2017 vol. 17(Issue 23) pp:3960-3978
Publication Date(Web):2017/11/21
DOI:10.1039/C7LC00627F
The intrinsic limitations of conventional batch synthesis have hindered its applications in both solving classical problems and exploiting new frontiers. Microfluidic technology offers a new platform for chemical synthesis toward either molecules or materials, which has promoted the progress of diverse fields such as organic chemistry, materials science, and biomedicine. In this review, we focus on the improved performance of microreactors in handling various situations, and outline the trend of microfluidic synthesis (microsynthesis, μSyn) from simple microreactors to integrated microsystems. Examples of synthesizing both chemical compounds and micro/nanomaterials show the flexible applications of this approach. We aim to provide strategic guidance for the rational design, fabrication, and integration of microdevices for synthetic use. We critically evaluate the existing challenges and future opportunities associated with this burgeoning field.
Co-reporter:Kaiwen Mou;Juanjuan Li;Yunyun Wang;Ruitao Cha
Journal of Materials Chemistry B 2017 vol. 5(Issue 38) pp:7876-7884
Publication Date(Web):2017/10/04
DOI:10.1039/C7TB01857F
Nanocellulose materials have undergone rapid development in recent years as promising biomedical materials due to their excellent physical and biological properties, in particular their biocompatibility, biodegradability, and low cytotoxicity. In this study, we prepared 2,3-dialdehyde nanofibrillated cellulose (DANFC) by sodium periodate oxidation, which is a mild oxidation process. With increasing oxidation time, the antimicrobial activity of DANFC against both Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA) improved. DANFC also displays good biocompatibility with mammalian cells, and shows good blood compatibility. In addition, animal studies and histology results reveal that DANFC can accelerate wound healing and enhance the formation of blood vessels and epithelium.
Co-reporter:Yan Feng, Wenwen Chen, Yuexiao Jia, Yue Tian, Yuyun Zhao, Fei Long, Yukui Rui and Xingyu Jiang
Nanoscale 2016 vol. 8(Issue 27) pp:13223-13227
Publication Date(Web):15 Jun 2016
DOI:10.1039/C6NR03317B
We demonstrate that N-heterocyclic molecule-capped gold nanoparticles (Au NPs) have broad-spectrum antibacterial activity. Optimized antibacterial activity can be achieved by using different initial molar ratios (1:1 and 10:1) of N-heterocyclic prodrugs and the precursor of Au NPs (HAuCl4). This work opens up new avenues for antibiotics based on Au NPs.
Co-reporter:Shaoling Cheng, Yapei Zhang, Ruitao Cha, Jinliang Yang and Xingyu Jiang
Nanoscale 2016 vol. 8(Issue 2) pp:973-978
Publication Date(Web):26 Nov 2015
DOI:10.1039/C5NR07647A
By mixing a guar gum (GG) solution with a nanocrystalline cellulose (NCC) dispersion using a novel circular casting technology, we manufactured biodegradable films as packaging materials with improved optical and mechanical properties. These films could act as barriers for oxygen and could completely dissolve in water within 5 h. We also compared the effect of nanocomposite films and commercial food packaging materials on the preservation of food.
Co-reporter:Yiping Chen, Jiashu Sun, Yunlei Xianyu, Binfeng Yin, Yajing Niu, Songbai Wang, Fengjing Cao, Xiaoqing Zhang, Yu Wang and Xingyu Jiang
Nanoscale 2016 vol. 8(Issue 33) pp:15205-15212
Publication Date(Web):20 Jun 2016
DOI:10.1039/C6NR04017A
Even though the gold lateral flow test (GLFT) is low-cost and allows for point-of-care testing (POCT), its intrinsic limitations including low sensitivity and incapability of quantification significantly hinder the clinical application of GLFT for assaying disease biomarkers. To improve the performance of the GLFT without sacrificing its simplicity, we develop a chemiluminescent-gold lateral flow test (C-mode GLFT) for quantitative and multiplex detection of disease biomarkers with an ultrahigh sensitivity at a picomolar level. Horseradish peroxidase (HRP) and antibody (Ab) are simultaneously labeled onto the surface of gold nanoparticles (AuNPs) to achieve a dual-readout (chemiluminescent and visual, C&V-mode GLFT). A red color appears at the test line caused by the accumulation of captured AuNPs in the presence of targets, while HRP on the surface of AuNPs catalyzes the chemiluminescence reaction of luminol to amplify the signal. C-mode GLFT is successfully used for detecting tumor biomarkers (alpha fetoprotein, AFP, and carcino embryonic antigen, CEA) and bacterial infection biomarkers (procalcitonin, PCT) in serum samples as well as whole blood. The excellent features of C-mode GLFT such as straightforward operation, ultrahigh sensitivity and quantitative detection, make it a promising platform for POCT of a variety of disease biomarkers in real samples.
Co-reporter:Qiang Feng, Jiashu Sun and Xingyu Jiang
Nanoscale 2016 vol. 8(Issue 25) pp:12430-12443
Publication Date(Web):26 Jan 2016
DOI:10.1039/C5NR07964K
The controlled synthesis of functional nanoparticles with tunable structures and properties has been extensively investigated for cancer treatment and diagnosis. Among a variety of methods for fabrication of nanoparticles, microfluidics-based synthesis enables enhanced mixing and precise fluidic modulation inside microchannels, thus allowing for the flow-mediated production of nanoparticles in a controllable manner. This review focuses on recent advances of using microfluidic devices for the synthesis of drug-loaded nanoparticles with specific characteristics (such as size, composite, surface modification, structure and rigidity) for enhanced cancer treatment and diagnosis as well as to investigate the bio-nanoparticle interaction. The discussion on microfluidics-based synthesis may shed light on the rational design of functional nanoparticles for cancer-related pharmaceutical applications.
Co-reporter:Yiping Chen, Yunlei Xianyu, Jiashu Sun, Yajing Niu, Yu Wang and Xingyu Jiang
Nanoscale 2016 vol. 8(Issue 2) pp:1100-1107
Publication Date(Web):07 Dec 2015
DOI:10.1039/C5NR07044A
This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.
Co-reporter:Yifeng Lei, Yoh Hamada, Jun Li, Liman Cong, Nuoxin Wang, Ying Li, Wenfu Zheng, Xingyu Jiang
Journal of Controlled Release 2016 Volume 232() pp:131-142
Publication Date(Web):28 June 2016
DOI:10.1016/j.jconrel.2016.03.023
Pancreatic cancer is a lethal malignancy whose progression is highly dependent on the nervous microenvironment. This study develops neural drug-loaded ferritin nanoparticles (Ft NPs) to regulate the nervous microenvironment, in order to control the pancreatic cancer progression. The drug-loaded Ft NPs can target pancreatic tumors via passive targeting of EPR effects of tumors and active targeting via transferrin receptor 1 (TfR1) binding on cancer cells, with a triggered drug release in acidic tumor environment. Two drugs, one activates neural activity (carbachol), the other impairs neural activity (atropine), are encapsulated into the Ft NPs to form two kinds of nano drugs, Nano-Cab NPs and Nano-Ato NPs, respectively. The activation of the nervous microenvironment by Nano-Cab NPs significantly promotes the pancreatic tumor progression, whereas the blockage of neural niche by Nano-Ato NPs remarkably impairs the neurogenesis in tumors and the progression of pancreatic cancer. The Ft-based nanoparticles thus comprise an effective and safe route of delivery of neural drugs for novel anti-cancer therapy.
Co-reporter:Wenshu Zheng, Huan Li, Wenwen Chen, Jian Ji, and Xingyu Jiang
Analytical Chemistry 2016 Volume 88(Issue 7) pp:4140
Publication Date(Web):March 9, 2016
DOI:10.1021/acs.analchem.6b00501
This report describes a colorimetric assay for trivalent metal cations (M3+) using gold nanoparticles (AuNPs)-modified with oppositely charged thiols that can form intermolecular zwitterionic surfaces. Zwitterionic AuNPs (Zw-AuNPs) are stable in high-salt solutions and well-dispersed in a wide range of pH values. M3+ including Fe3+, Al3+, and Cr3+ can effectively trigger the aggregation of Zw-AuNPs by interfering with their surface potential, and aggregated AuNPs can be regenerated and recycled by removing M3+. In our approach, the output signal can be observed by the naked eye within a micromolar (μM) concentration range. Uniquely, our assay is capable of discriminating Fe3+ from Fe2+, which is challenging using traditional approaches. More importantly, Zw-AuNPs can be stored stably at room temperature for a long period (3 months) with constant detection performance. Both the cost-effectiveness and the long shelf life make Zw-AuNPs ideal for detecting M3+ in resource-poor and remote areas.
Co-reporter:Yiping Chen, Yunlei Xianyu, Jing Wu, Wenfu Zheng, Jianghong Rao, and Xingyu Jiang
Analytical Chemistry 2016 Volume 88(Issue 11) pp:5605
Publication Date(Web):May 5, 2016
DOI:10.1021/acs.analchem.6b01122
The illegal addition of β-lactamase (Bla) in milk to disguise β-lactam antibiotics has been a serious issue in the milk industry worldwide. Herein, we report a method for point-of-care detection of Bla based on a probe, Tokyo Green-tethered β-lactam (CDG-1), as a common substrate of various Blas (Bla A, B...) which can enzymatically convert CDG-1 (low fluorescence) to Tokyo Green (high fluorescence). This approach allows rapid screening of a broad spectrum of Blas in real milk samples within 15 min without any pretreatment. Combined with the immuno-magnetic separation, we achieved sensitive and quantitative detection of Bla (10–5 U/mL), which provides a universal platform for screening and determining Blas in complex samples with high efficiency and accuracy.
Co-reporter:Wenshu Zheng and Xingyu Jiang
Analyst 2016 vol. 141(Issue 4) pp:1196-1208
Publication Date(Web):13 Jan 2016
DOI:10.1039/C5AN02222C
The boom of nanotechnology has yielded exciting developments in designing new kinds of colorimetric immunoassays. These nanomaterial-associated immunoassays have shown great potential for clinical translation and a number of them have already been implemented for testing patient samples from the clinics. Different from most reviews where researchers typically focus on a specific type of nanomaterial or describe assays based on the types of materials, we classify these assays by the function of nanomaterials, focusing on reviewing the distinct phenomenon of nanomaterials and how these properties are utilized to overcome limitations faced by traditional colorimetric immunoassays. We also discuss the challenges and give our perspectives in this field.
Co-reporter:Huashan Wang, Juanjuan Li, Xiaoqing Zhang, Binfeng Hu, Yang Liu, Lin Zhang, Ruitao Cha, Jiashu Sun and Xingyu Jiang
Analyst 2016 vol. 141(Issue 3) pp:815-819
Publication Date(Web):24 Nov 2015
DOI:10.1039/C5AN01835H
We demonstrate a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay (MIC) for multiple, sensitive, reliable and rapid detection of testosterone in human serum and urine samples. As MIC can detect biomarkers in a cost-effective and easy-to-operate manner, it may have great potential for clinical diagnosis and point-of-care testing (POCT).
Co-reporter:Yifeng Lei, Jun Li, Nuoxin Wang, Xinglong Yang, Yoh Hamada, Qizhai Li, Wenfu Zheng and Xingyu Jiang
Integrative Biology 2016 vol. 8(Issue 3) pp:359-367
Publication Date(Web):23 Feb 2016
DOI:10.1039/C5IB00309A
Emerging evidence suggests that there is extensive interaction between neurons and cancer cells. However, few model systems have been developed to investigate nerve–cancer cell interaction in vitro. Herein, a high-throughput microfluidic compartmentalized chip is developed to examine the interaction between neurons and cancer cells. The nerve bundles appear to provide a biophysical support for cancer cells and guide their directional migration. The cancers that have high levels of perineural invasion in clinical observations exhibit greater migration along neurites in the on-chip model. The on-chip model allows the screening of compounds which inhibit cancer cell migration along neurites in vitro. The interruption of neurites, the pharmacological blockade of nerve–cancer signaling, effectively attenuates the migration of cancer cells along neurites. This on-chip model provides a useful platform to investigate the dynamic interaction between cancer cells and neurons and can dramatically broaden the chemical space in screening neuron-related drugs for cancers.
Co-reporter:Nuoxin Wang, Lixue Tang, Wenfu Zheng, Yunhu Peng, Shiyu Cheng, Yifeng Lei, Lingmin Zhang, Bingfeng Hu, Shaoqin Liu, Wei Zhang and Xingyu Jiang
RSC Advances 2016 vol. 6(Issue 60) pp:55054-55063
Publication Date(Web):01 Jun 2016
DOI:10.1039/C6RA12768A
We develop a rapid and facile method to fabricate tubular scaffolds by a single-step rolling operation. With the aid of fibrin medical glue and a smooth expanded polytetrafluoroethylene (ePTFE) mandrel, we can wrap a piece of flat thin film into a three-dimensional (3D), multi-layered tubular structure with well-controlled diameter, wall thickness, and mechanical strength within 10 min. By patterning different cells in a pre-designed area on the film, after rolling, we can obtain blood vessel-mimicking tissues with well-arranged, multilayered 3D architectures within 70 min. Our strategy provides an excellent platform to rapidly fabricate tubular scaffolds essentially with no equipment and straightforward manipulations.
Co-reporter:Juanjuan Li, Yang Liu, Ruitao Cha, Bei Ran, Kaiwen Mou, Huashan Wang, Qian Xie, Jiashu Sun and Xingyu Jiang
RSC Advances 2016 vol. 6(Issue 17) pp:14329-14334
Publication Date(Web):28 Jan 2016
DOI:10.1039/C5RA25729H
This paper reports a new synthesis method to control the size of iron oxide nanoparticles (IONs) by adding sodium citrate during fabrication to obtain sodium citrate-modified iron oxide nanoparticles (SCIONs). The method was simpler and more effective than the synthetic process of ferumoxytol, a commercial nano-sized iron agent. Compared with other iron agents which were prepared using branched polymers to form a carbohydrate shell, our SCIONs were tightly bound within a nonionic carbohydrate matrix. The physicochemical properties of SCIONs were characterized, and the results showed that these nanoparticles could be stably stored in water for six months without sedimentation. The cytotoxicity evaluation of SCIONs indicated that they were biocompatible to cells. The effect of iron supply by SCIONs was assessed by measuring the retention of iron ions in the serum, and the results demonstrated that the synthesized SCIONs are very promising for intravenous iron supply.
Co-reporter:Ye Liu, Huaimin Wang, Dan Li, Yue Tian, Wenwen Liu, Lingmin Zhang, Wenshu Zheng, Yanling Hao, Jiandong Liu, Zhimou Yang, Yiming Shao and Xingyu Jiang
Nanoscale Horizons 2016 vol. 1(Issue 2) pp:135-143
Publication Date(Web):14 Dec 2015
DOI:10.1039/C5NH00064E
Herein, we report that the in situ formed peptidic nanofibers facilitate the induction of multiple crucial immunities against HIV DNA vaccine, including polyfunctional T cell response, broad IgG subclasses response, and V1/V2 loop-specific antibody response, all of which can hardly be triggered by HIV DNA vaccine alone. Such novel in situ formation fundamentally overcomes the big hurdle for the applications of such nanofibers, which previously can only trigger these crucial immune responses via adding exogenous alkaline phosphatase. Such robustness of peptidic nanofibers for inducing crucial immune responses may allow better inhibition against HIV than reported materials.
Co-reporter:Shiwen Wang, Jiashu Sun, Yuexiao Jia, Lu Yang, Nuoxin Wang, Yunlei Xianyu, Wenwen Chen, Xiaohong Li, Ruitao Cha, and Xingyu Jiang
Biomacromolecules 2016 Volume 17(Issue 7) pp:
Publication Date(Web):June 22, 2016
DOI:10.1021/acs.biomac.6b00642
Nanocrystalline cellulose (NCC) is a kind of natural biopolymers with merits of large surface area, high specific strength and unique optical properties. This report shows that NCC can serve as the substrate, allowing glucose to reduce Tollen’s reagent to produce silver nanoparticles (AgNPs) at room temperature. The generation of AgNPs is affected by the factors such as the concentrations of silver ions, NCC and glucose, as well as the different reaction temperatures. The AgNPs with NCC are applied for the development of a visual, quantitative, nonenzymatic and high-sensitive assay for glucose detection in serum. This assay is also used for monitoring the concentration change of glucose in medium during cell culture. For the antibacterial activity, the minimal inhibitory concentration (MIC) of the generated AgNPs with NCC is much lower than that of commercial AgNPs, attributed to the good dispersion of AgNPs with the presence of NCC. As NCC exhibits unique advantages including green, stable, inexpensive, and abundant, the NCC-based generation of AgNPs may find promising applications in clinical diagnosis, environmental monitoring, and the control of bacteria.
Co-reporter:Qiang Feng, Lu Zhang, Jiashu Sun, Xingyu Jiang
Nanomedicine: Nanotechnology, Biology and Medicine 2016 Volume 12(Issue 2) pp:460
Publication Date(Web):February 2016
DOI:10.1016/j.nano.2015.12.039
Co-reporter:Shiwen Wang, Qiang Feng, Jiashu Sun, Feng Gao, Wei Fan, Zhong Zhang, Xiaohong Li, and Xingyu Jiang
ACS Nano 2016 Volume 10(Issue 1) pp:298
Publication Date(Web):December 19, 2015
DOI:10.1021/acsnano.5b04393
The doping of biocompatible nanomaterials into ultrahigh molecular weight polyethylene (UHMWPE) to improve the biocompatibility and reduce the wear debris is of great significance to prolonging implantation time of UHMWPE as the bearing material for artificial joints. This study shows that UHMWPE can form a composite with nanocrystalline cellulose (NCC, a hydrophilic nanosized material with a high aspect ratio) by ball-milling and hot-pressing. Compared to pure UHMWPE, the NCC/UHMWPE composite exhibits improved tribological characteristics with reduced generation of wear debris. The underlying mechanism is related to the weak binding between hydrophilic NCC and hydrophobic UHMWPE. The hydrophilic, rigid NCC particles tend to detach from the UHMWPE surface during friction, which could move with the rubbing surface, serve as a thin lubricant layer, and protect the UHMWPE substrate from abrasion. The biological safety of the NCC/UHMWPE composite, as tested by MC3T3-E1 preosteoblast cells and macrophage RAW264.7 cells, is high, with significantly lower inflammatory responses/cytotoxicity than pure UHMWPE. The NCC/UHMWPE composite therefore could be a promising alternative to the current UHMWPE for bearing applications.Keywords: artificial joint; debris; friction; nanocrystalline cellulose; ultrahigh molecular weight polyethene;
Co-reporter:Wenjing Lu, Yiping Chen, Zhong Liu, Wenbo Tang, Qiang Feng, Jiashu Sun, and Xingyu Jiang
ACS Nano 2016 Volume 10(Issue 7) pp:6685
Publication Date(Web):June 27, 2016
DOI:10.1021/acsnano.6b01903
One-step, quantitative and rapid detection of microRNA (miRNA) in tumor cells or tissues can provide critical information for clinical diagnosis and cancer treatment. In this work, we develop a magnetic relaxation switch sensing (MRS)-based miRNA sensor using magnetic microparticle (1 μm in diameter, MM1000)-DNA probe-magnetic nanoparticle (30 nm in diameter, MN30) conjugates (MM1000-DNA-MN30). In the presence of target miRNA, DSN enzyme selectively cleaves the DNA tether after miRNA/DNA hybridization to release MN30 and leaves the miRNA intact to lead to the declustering of more MN30 than before. In contrast to conventional MRS by measuring the change of transverse relaxation time (ΔT2) induced by the aggregation or dissociation of magnetic particles in the presence of target, we use the cleaved MN30 from conjugates as the direct readout of ΔT2, which is more sensitive and stable. This MRS-based assay allows for one-step detection of 5 fM of miR-21 in urine samples, quantification of miR-21 from 100 cancer cells, and differentiation of the expression of miR-21 in tumor and surrounding tissues. The merits of this assay, rapidity, ability for quantitation, high sensitivity, and one-step operation, ensure a promising future in diagnostic technology.Keywords: detection; magnetic nanoparticles; magnetic relaxation switch; miRNA; tumor
Co-reporter:Ye Liu, Yekkuni L Balachandran, Dan Li, Yiming Shao, and Xingyu Jiang
ACS Nano 2016 Volume 10(Issue 3) pp:3589
Publication Date(Web):February 4, 2016
DOI:10.1021/acsnano.5b08025
One of the biggest obstacles for the development of HIV vaccines is how to sufficiently trigger crucial anti-HIV immunities via a safe manner. We herein integrated surface modification-dependent immunostimulation against HIV vaccine and shape-dependent biosafety and designed a safe noncarrier adjuvant based on silver nanorods coated by both polyvinylpyrrolidone (PVP) and polyethylene glycol (PEG). Such silver nanorods can significantly elevate crucial immunities of HIV vaccine and overcome the toxicity, which is a big problem for other existing adjuvants. This study thus provided a principle for designing a safe and high-efficacy material for an adjuvant and allow researchers to really have a safe and effective prophylaxis against HIV. We expect this material approach to be applicable to other types of vaccines, whether they are preventative or therapeutic.Keywords: HIV vaccine; immune response; noncarrier adjuvant; poly(ethylene glycol); polyvinylpyrrolidone; silver nanorod
Co-reporter:Yi Zhang;Xianhong Huang;Wenwen Liu;Guanxin Zhang
Science China Chemistry 2016 Volume 59( Issue 1) pp:106-113
Publication Date(Web):2016 January
DOI:10.1007/s11426-015-5543-2
Hydrogen sulfide (H2S) has been found to be the third most important endogenous gaseous signaling molecule after nitric oxide (NO) and carbonic oxide (CO) and plays crucial roles in living organisms and biological systems. Here we use aggregation- induced emission (AIE) of a small organic molecule (TPE-indo) to detect H2S in both solution and living cells. TPE-indo can target mitochondria and aggregate to fluoresce, which can serve as a sensor for monitoring H2S in the mitochondria. We regulate the fluorescence of AIE molecules by tuning the viscosity of the solution to form TPE-indo nanoparticles, constructing a probe for H2S with good selectivity and high sensitivity. The nucleophilic addition of HS- to the TPE-indo is crucial for the rapid H2S detection. The imaging and analysis of H2S in mitochondria of living cells with the probe demonstrate potential biological applications.
Co-reporter:Yunyun Wang, Ye Liu, Xinli Deng, Yulong Cong, Xingyu Jiang
Biosensors and Bioelectronics 2016 Volume 86() pp:211-218
Publication Date(Web):15 December 2016
DOI:10.1016/j.bios.2016.06.021
•A novel readout mode of ELISA based on the binding between peptidic β-sheet and Congo Red.•Such method significantly optimizes the error variance and the sensitivity of ELISA.•This study provides a superior option for precisely diagnosing P-Selectin related thrombus diseases.Although conventional enzyme-linked immunosorbent assays (ELISA) and related assays have been widely applied for the diagnosis of diseases, many of them suffer from large error variance for monitoring the concentration of targets over time, and insufficient limit of detection (LOD) for assaying dilute targets. We herein report a readout mode of ELISA based on the binding between peptidic β-sheet structure and Congo Red. The formation of peptidic β-sheet structure is triggered by alkaline phosphatase (ALP). For the detection of P-Selectin which is a crucial indicator for evaluating thrombus diseases in clinic, the ‘β-sheet and Congo Red’ mode significantly decreases both the error variance and the LOD (from 9.7 ng/ml to 1.1 ng/ml) of detection, compared with commercial ELISA (an existing gold-standard method for detecting P-Selectin in clinic). Considering the wide range of ALP-based antibodies for immunoassays, such novel method could be applicable to the analysis of many types of targets.
Co-reporter:Wenjing Lu, Jidong Wang, Qiong Wu, Jiashu Sun, Yiping Chen, Lu Zhang, Chunsheng Zheng, Wenna Gao, Yi Liu, Xingyu Jiang
Biosensors and Bioelectronics 2016 Volume 75() pp:28-33
Publication Date(Web):15 January 2016
DOI:10.1016/j.bios.2015.08.016
•MTNT enables high-throughput detection of both DNA and RNA from crude samples.•MTNT has an excellent sensitivity and specificity, with the LOD of 2 copies.•MTNT can detect mRNA from as few as 2 cancer cells without complicated procedures.We develop a micro-pipette tip-based nucleic acid test (MTNT) for high-throughput sample-to-answer detection of both DNA and RNA from crude samples including cells, bacteria, and solid plants, without the need of sample pretreatment and complex operation. MTNT consists of micro-pipette tips and embedded solid phase nucleic acid extraction membranes, and fully integrates the functions of nucleic acid extraction from crude samples, loop-mediated isothermal amplification (LAMP) of nucleic acids, and visual readout of assays. The total assaying time for DNA or RNA from a variety of crude samples ranges from 90 to 160 min. The limit of detection (LOD) of MTNT is 2 copies of plasmids containing the target nucleic acid fragments of Ebola virus, and 8 CFU of Escherichia coli carrying Ebola virus-derived plasmids. MTNT can also detect CK-19 mRNA from as few as 2 cancer cells without complicated procedures such as RNA extraction and purification. We further demonstrate MTNT in a high-throughput format using an eight-channel pipette and a homemade mini-heater, with a maximum throughput of 40 samples. Compared with other point-of-care (POC) nucleic acid tests (NAT), MTNT could assay both DNA and RNA directly from liquid (cells/bacteria/blood) or solid (plant) samples in a straightforward, sensitive, high-throughput, and containment-free manner, suggesting a considerable promise for low-cost and POC NAT in remote areas.
Co-reporter:Jiashu Sun;Lu Zhang;Jiuling Wang;Qiang Feng;Dingbin Liu;Qifang Yin;Dongyan Xu;Yujie Wei;Baoquan Ding;Xinghua Shi
Advanced Materials 2015 Volume 27( Issue 8) pp:1402-1407
Publication Date(Web):
DOI:10.1002/adma.201404788
Co-reporter:Mengmeng Li, Shiwen Wang, Jia Jiang, Jiashu Sun, Yuzhuo Li, Deyong Huang, Yun-Ze Long, Wenfu Zheng, Shiyi Chen and Xingyu Jiang
Nanoscale 2015 vol. 7(Issue 17) pp:8071-8075
Publication Date(Web):01 Apr 2015
DOI:10.1039/C5NR01439E
The Ligament Advanced Reinforcement System (LARS) has been considered as a promising graft for ligament reconstruction. To improve its biocompatibility and effectiveness on new bone formation, we modified the surface of a polyethylene terephthalate (PET) ligament with nanoscale silica using atom transfer radical polymerization (ATRP) and silica polymerization. The modified ligament is tested by both in vitro and in vivo experiments. Human osteoblast testing in vitro exhibits an ∼21% higher value in cell viability for silica-modified grafts compared with original grafts. Animal testing in vivo shows that there is new formed bone in the case of a nanoscale silica-coated ligament. These results demonstrate that our approach for nanoscale silica surface modification on LARS could be potentially applied for ligament reconstruction.
Co-reporter:Wenwen Chen, Fengjing Cao, Wenshu Zheng, Yue Tian, Yunlei Xianyu, Peng Xu, Wei Zhang, Zhuo Wang, Ke Deng and Xingyu Jiang
Nanoscale 2015 vol. 7(Issue 5) pp:2042-2049
Publication Date(Web):15 Dec 2014
DOI:10.1039/C4NR06726F
We report a method for rapid, effective detection of both Cr(III) and Cr(VI) (in the form of Cr3+ and Cr2O72−, the main species of chromium in the natural environment) by making use of meso-2,3-dimercaptosuccinic acid (DMSA)-functionalized gold nanoparticles (Au NPs). The limit of detection (LOD) is 10 nM with the naked eye and the assay can be applied in detecting chromium in polluted soil from Yun-Nan Province in Southwest China. We use density functional theory to calculate the change of the Gibbs free energy (ΔG) of the interactions between the DMSA-Au NP system and various metal ions, which shows that DMSA-Au NPs have high specificity for both Cr3+ and Cr2O72−.
Co-reporter:Ying Li, Hua Jiang, Wenfu Zheng, Niya Gong, Lili Chen, Xingyu Jiang and Guang Yang
Journal of Materials Chemistry A 2015 vol. 3(Issue 17) pp:3498-3507
Publication Date(Web):17 Mar 2015
DOI:10.1039/C4TB01819B
Bacterial cellulose (BC), a network of pure cellulose nanofibers with fine crystallinity, high mechanical strength and wet capability, and good biocompatibility, is a good material candidate for wound dressing. Hyaluronan (HA) has obvious curative properties, promoting the healing of wound skin tissue and reducing scar formation. This study explored an “orifice plate” culture method to obtain BC samples of different sizes but consistent thicknesses. Novel BC–HA nanocomposites with a 3-D network structure were obtained through a solution impregnation method. The total surface area and the pore volume of the BC–HA composite films gradually decreased with the increase of HA content. The elongation of BC–HA composite films at the break point gradually increased as the HA content increased while the tensile strength of the BC–HA composite films decreased during the same process. The BC–HA composite films had a better water uptake capability than pure BC, and water vapor transmission rate (WVTR) measurements showed that the BC–HA composite films can satisfy breathing requirements of injured skin. The BC–HA composite films facilitated the growth of primary human fibroblast cells, showing their low toxicity, and the BC–HA composite films with 0.1% HA lead to higher levels of cell viability than the pure BC. In vivo experiments indicated that the BC–HA with 0.1% HA had the shortest wound healing time while BC–HA with 0.05% HA yielded best tissue repair results. The BC–HA composite films are expected to be useful as novel wound dressing materials for clinical skin repair.
Co-reporter:Yunlei Xianyu, Yiping Chen, and Xingyu Jiang
Analytical Chemistry 2015 Volume 87(Issue 21) pp:10688
Publication Date(Web):October 13, 2015
DOI:10.1021/acs.analchem.5b03522
This report outlines an enzymatic cascade reaction for signal transduction and amplification for plasmonic immunoassays by using horseradish peroxidase (HRP)-mediated aggregation of gold nanoparticles (AuNPs). HRP-catalyzed oxidation of iodide and iodide-catalyzed oxidation of cysteine is employed to modulate the plasmonic signals of AuNPs. It agrees well with the current immunoassay platforms and allows naked-eye readout with enhanced sensitivity, which holds great promise for applications in resource-constrained settings.
Co-reporter:Sha He, Yi Zhang, Pei Wang, Xingzhi Xu, Kui Zhu, Wenying Pan, Wenwen Liu, Kaiyong Cai, Jiashu Sun, Wei Zhang and Xingyu Jiang
Lab on a Chip 2015 vol. 15(Issue 1) pp:105-112
Publication Date(Web):07 Oct 2014
DOI:10.1039/C4LC00901K
This work develops a high-throughput, high-efficiency and straightforward microfluidic blotting method for analyzing proteins and nucleic acids. Sample solutions containing antibodies (for protein detection) or hybridization probes (for nucleic acid detection) are introduced into the parallel, serpentine microchannels to specifically recognize the immobilized targets on the substrate, achieving the identification of multiple targets in multiple samples simultaneously. The loading control, molecular weight markers, and antigen/antibody titration are designed and integrated into the microfluidic chip, thus allowing for the quantification of proteins and nucleic acids. Importantly, we could easily distinguish the adjacent blotting bands inside parallel microchannels, which may be difficult to achieve in conventional blotting. The small dimensions of microfluidic channels also help to reduce the amount of probing molecules and to accelerate the biochemical reaction. Our microfluidic blotting could bypass the steps of blocking and washing, further reducing the operation time and complexity.
Co-reporter:Yi Zhang, Jiashu Sun, Yu Zou, Wenwen Chen, Wei Zhang, Jianzhong Jeff Xi, and Xingyu Jiang
Analytical Chemistry 2015 Volume 87(Issue 2) pp:900
Publication Date(Web):December 16, 2014
DOI:10.1021/ac5032379
Multiplexed assay of analytes is of great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode and decode may realize this goal in a straightforward and consistent manner. We present here a microfluidic barcoded chip containing several sets of microchannels with different widths, imitating the commonly used barcode. A single barcoded microchip can carry out tens of individual protein/nucleic acid assays (encode) and immediately yield all assay results by a portable barcode reader or a smartphone (decode). The applicability of a barcoded microchip is demonstrated by human immunodeficiency virus (HIV) immunoassays for simultaneous detection of three targets (anti-gp41 antibody, anti-gp120 antibody, and anti-gp36 antibody) from six human serum samples. We can also determine seven pathogen-specific oligonucleotides by a single chip containing both positive and negative controls.
Co-reporter:A. S. Andersen, W. F. Zheng, D. S. Sutherland and X. Y. Jiang
Lab on a Chip 2015 vol. 15(Issue 24) pp:4524-4532
Publication Date(Web):20 Oct 2015
DOI:10.1039/C5LC00916B
A novel approach combining self-assembly-based colloidal lithography and polydimethylsiloxane (PDMS) micromolding to generate complex protein nanopatterns for studying the mechanisms of leukocyte extravasation within microchannels is presented. Nanostructured surfaces sealed onto PDMS-molded microchannels are chemically functionalized in situ in an all-aqueous process to generate bi-functional chemical nanopatterns. Subsequent co-immobilization with proteins makes use of common non-covalent coupling (e.g. HIS-tags, FC-tags and biotin-tags), giving nanopatterns of arbitrary combinations of oriented, functional proteins. Up to three different proteins were simultaneously co-immobilized into the microchannel with nanoscale precision, demonstrating the complex patterns. As a proof-of-principle, a mimic of an inflamed endothelium was constructed using a macro- and nanoscale pattern of intercellular adhesion molecule 1 (ICAM1) and P-selectin, and the response of leukocytes through live cell imaging was measured. A clear result on the rolling behavior of the cells was observed with rolling limited to areas where ICAM1 and P-selectin are present. This micro/nano-interface will open new doors to investigations of how spatial distributions of proteins control cellular activity.
Co-reporter:Lu Zhang, Wenwen Liu, Xianhong Huang, Guanxin Zhang, Xuefei Wang, Zhuo Wang, Deqing Zhang and Xingyu Jiang
Analyst 2015 vol. 140(Issue 17) pp:5849-5854
Publication Date(Web):19 Jun 2015
DOI:10.1039/C5AN00918A
Here, we explore the new application of an old molecule. We find that the tetraphenylethene-indolium molecule (TPE-indo) can both image the mitochondria (in the aggregated state), and indicate mitochondrial activity by the fluorescence change of TPE-indo. TPE-indo shows good photostability, longer emission wavelength, targeting effect for mitochondria, and better response to the changes of the mitochondrial membrane potential (ΔΨm).
Co-reporter:Jun Li;Yifeng Lei;Chun-Lin Sun;Wenfu Zheng;Hao-Li Zhang
Advanced Materials Interfaces 2015 Volume 2( Issue 15) pp:
Publication Date(Web):
DOI:10.1002/admi.201500335
Conformational changes of peptides are critically important in the control of their biological activities. Here, a quaternary ammonium group-terminated RGD-containing peptide (RGD-NMe3) is designed, which may undergo reversible conformational switch upon different electrochemical potentials. Potential responsive peptide interfaces are constructed on gold substrates with RGD-NMe3 in a tetra (ethylene glycol) background. It is demonstrated that by applying positive and negative potentials, the RGD peptide can be reversibly switched between linear and cyclic conformation, which can be used in reversible controlling of cell adhesion/migration on the interface. Furthermore, by combining microfluidics, adhesion of the cells in specific areas on the surface and subsequent directional migration of the cells can be controlled. It is believed that this straightforward potential modulation mechanism for peptide conformation control may find a wide use in design responsive peptide interfaces.
Co-reporter:Wenwen Chen, Yuexiao Jia, Yan Feng, Wenshu Zheng, Zhuo Wang and Xingyu Jiang
RSC Advances 2015 vol. 5(Issue 76) pp:62260-62264
Publication Date(Web):14 Jul 2015
DOI:10.1039/C5RA09099G
We synthesized ionic liquid (1-ethyl-3-methylimidazolium thiocyanate)-coated gold nanoparticles (IL-Au NPs) using a one-pot method and introduced them in the colorimetric assay for Al3+. IL-Au NPs showed good sensitivity and selectivity. They can determine the concentration of aluminum in vermicelli samples.
Co-reporter:Yiping Chen, Yunlei Xianyu, Yu Wang, Xiaoqing Zhang, Ruitao Cha, Jiashu Sun, and Xingyu Jiang
ACS Nano 2015 Volume 9(Issue 3) pp:3184
Publication Date(Web):March 6, 2015
DOI:10.1021/acsnano.5b00240
We report a sensing methodology that combines magnetic separation (MS) and magnetic relaxation switching (MS-MRS) for one-step detection of bacteria and viruses with high sensitivity and reproducibility. We first employ a magnetic field of 0.01 T to separate the magnetic beads of large size (250 nm in diameter) from those of small size (30 nm in diameter) and use the transverse relaxation time (T2) of the water molecules around the 30 nm magnetic beads (MB30) as the signal readout of the immunoassay. An MS-MRS sensor integrates target enrichment, extraction, and detection into one step, and the entire immunoassay can be completed within 30 min. Compared with a traditional MRS sensor, an MS-MRS sensor shows enhanced sensitivity, better reproducibility, and convenient operation, thus providing a promising platform for point-of-care testing.Keywords: homogeneous immunosensor; magnetic bead; magnetic relaxation switch; magnetic separation; one-step detection;
Co-reporter:Lu Zhang, Qiang Feng, Jiuling Wang, Shuai Zhang, Baoquan Ding, Yujie Wei, Mingdong Dong, Ji-Young Ryu, Tae-Young Yoon, Xinghua Shi, Jiashu Sun, and Xingyu Jiang
ACS Nano 2015 Volume 9(Issue 10) pp:9912
Publication Date(Web):October 8, 2015
DOI:10.1021/acsnano.5b05792
The functionalized lipid shell of hybrid nanoparticles plays an important role for improving their biocompatibility and in vivo stability. Yet few efforts have been made to critically examine the shell structure of nanoparticles and its effect on cell–particle interaction. Here we develop a microfluidic chip allowing for the synthesis of structurally well-defined lipid-polymer nanoparticles of the same sizes, but covered with either lipid-monolayer-shell (MPs, monolayer nanoparticles) or lipid-bilayer-shell (BPs, bilayer nanoparticles). Atomic force microscope and atomistic simulations reveal that MPs have a lower flexibility than BPs, resulting in a more efficient cellular uptake and thus anticancer effect than BPs do. This flexibility-regulated cell–particle interaction may have important implications for designing drug nanocarriers.Keywords: drug deliver; interfaces; lipids; microfluidics; nanostructures;
Co-reporter:Ying Li, Shiwen Wang, Rong Huang, Zhuo Huang, Binfeng Hu, Wenfu Zheng, Guang Yang, and Xingyu Jiang
Biomacromolecules 2015 Volume 16(Issue 3) pp:
Publication Date(Web):January 28, 2015
DOI:10.1021/bm501680s
Bacterial cellulose (BC) is a kind of nanobiomaterial for tissue engineering. How the nanoscale structure of BC affects skin wound repair is unexplored. Here, the hierarchical structure of BC films and their different effects on skin wound healing were studied both in vitro and in vivo. The bottom side of the BC film had a larger pore size, and a looser and rougher structure than that of the top side. By using a microfluidics-based in vitro wound healing model, we revealed that the bottom side of the BC film can better promote the migration of cells to facilitate wound healing. Furthermore, the full-thickness skin wounds on Wistar rats demonstrated that, compared with gauze and the top side of the BC film, the wound covered by the bottom side of the BC film showed faster recovery rate and less inflammatory response. The results indicate that the platform based on the microfluidic chip provide a rapid, reliable, and repeatable method for wound dressing screening. As an excellent biomaterial for wound healing, the BC film displays different properties on different sides, which not only provides a method to optimize the biocompatibility of wound dressings but also paves a new way to building heterogeneous BC-based biomaterials for complex tissue engineering.
Co-reporter:Jiashu Sun, Yunlei Xianyu and Xingyu Jiang
Chemical Society Reviews 2014 vol. 43(Issue 17) pp:6239-6253
Publication Date(Web):02 Jun 2014
DOI:10.1039/C4CS00125G
One of the goals of point-of-care (POC) is a chip-based, miniaturized, portable, self-containing system that allows the assay of proteins, nucleic acids, and cells in complex samples. The integration of nanomaterials and microfluidics can help achieve this goal. This tutorial review outlines the mechanism of assaying biomarkers by gold nanoparticles (AuNPs), and the implementation of AuNPs for microfluidic POC devices. In line with this, we discuss some recent advances in AuNP-coupled microfluidic sensors with enhanced performance. Portable and automated instruments for device operation and signal readout are also included for practical applications of these AuNP-combined microfluidic chips.
Co-reporter:B. Sun, Y.Z. Long, H.D. Zhang, M.M. Li, J.L. Duvail, X.Y. Jiang, H.L. Yin
Progress in Polymer Science 2014 Volume 39(Issue 5) pp:862-890
Publication Date(Web):May 2014
DOI:10.1016/j.progpolymsci.2013.06.002
Compared with other nanofiber fabrication processes, electrospinning is versatile and superior in production and construction of ordered or more complex nanofibrous assemblies. Besides traditional two-dimensional (2D) nanofibrous structures, electrospinning is powerful in fabrication of three-dimensional (3D) fibrous macrostructures, especially for tissue engineering applications. This article summarizes and reviews recent advances in various promising and cutting-edge electrospinning techniques, including multilayering electrospinning, post-processing after electrospinning, liquid-assisted collection, template-assisted collection, porogen-added electrospinning, and self-assembly. And their formation mechanisms, features, and the challenges of electrospinning have also been discussed. Furthermore, these 3D nanofibrous macrostructures have been demonstrated to have potential applications in tissue engineering, energy harvesting and storage, and filtration.
Co-reporter:Yue Tian, Huaimin Wang, Ye Liu, Lina Mao, Wenwen Chen, Zhening Zhu, Wenwen Liu, Wenfu Zheng, Yuyun Zhao, Deling Kong, Zhimou Yang, Wei Zhang, Yiming Shao, and Xingyu Jiang
Nano Letters 2014 Volume 14(Issue 3) pp:1439-1445
Publication Date(Web):February 24, 2014
DOI:10.1021/nl404560v
This report shows that a nanovector composed of peptide-based nanofibrous hydrogel can condense DNA to result in strong immune responses against HIV. This nanovector can strongly activate both humoral and cellular immune responses to a balanced level rarely reported in previous studies, which is crucial for HIV prevention and therapy. In addition, this nanovector shows good biosafety in vitro and in vivo. Detailed characterizations show that the nanofibrous structure of the hydrogel is critical for the dramatically improved immune responses compared to existing materials. This peptide-based nanofibrous hydrogel shows great potential for efficacious HIV DNA vaccines and can be potentially used for delivering other vaccines and drugs.
Co-reporter:Shiwen Wang, Wei Chen, Sha He, Qilong Zhao, Xiaohong Li, Jiashu Sun and Xingyu Jiang
Nanoscale 2014 vol. 6(Issue 12) pp:6468-6472
Publication Date(Web):18 Apr 2014
DOI:10.1039/C4NR01166J
In this paper, we present a simple but efficient biomimetic method to encapsulate laccase on mesoporous silica-modified electrospun (ES) ultrafine fibers. Because of the mild immobilization conditions (room temperature, aqueous condition), the encapsulated laccase retained a high activity of 94%. Because of the protection from the silica layer, the laccase worked efficiently at 60 °C and retained a long-term activity in the presence of proteinase K. After recycling for 10 times the laccase still preserved 96% of its original reactivity. More remarkably, the immobilized laccase on fibers could completely recover its activity after thermal denature, while the free laccase permanently lost the activity. We also demonstrated that the laccase on silica-coated fibers exhibited an enhanced decolorization capability of Brilliant Blue KN-R (BBKN-R) as compared to the free laccase, showing its great potential for industrial applications.
Co-reporter:Wenjing Lu, Jiashu Sun and Xingyu Jiang
Journal of Materials Chemistry A 2014 vol. 2(Issue 17) pp:2369-2380
Publication Date(Web):24 Jan 2014
DOI:10.1039/C3TB21478H
Electrospinning technology underwent rapid development in recent years, which can be used for fabricating electrospun fibers with different morphologies and multidimensional structures. These fibers are widely applied in medical diagnosis, tissue engineering, replica molding and other applications. Here we review the recent advances in the electrospinning technology, especially technical progress in fabricating electrospun fibers and assemblies with multidimensional structures, and the biomedical applications of these fibers.
Co-reporter:Yue Tian, Juanjuan Qi, Wei Zhang, Qiang Cai, and Xingyu Jiang
ACS Applied Materials & Interfaces 2014 Volume 6(Issue 15) pp:12038
Publication Date(Web):July 22, 2014
DOI:10.1021/am5026424
In this study, we exploit a facile, one-pot method to prepare MCM-41 type mesoporous silica nanoparticles decorated with silver nanoparticles (Ag-MSNs). Silver nanoparticles with diameter of 2–10 nm are highly dispersed in the framework of mesoporous silica nanoparticles. These Ag-MSNs possess an enhanced antibacterial effect against both Gram-positive and Gram-negative bacteria by preventing the aggregation of silver nanoparticles and continuously releasing silver ions for one month. The cytotoxicity assay indicates that the effective antibacterial concentration of Ag-MSNs shows little effect on human cells. This report describes an efficient and economical route to synthesize mesoporous silica nanoparticles with uniform silver nanoparticles, and these nanoparticles show promising applications as antibiotics.Keywords: antibacterial activity; continuously release; highly dispersity; mesoporous silica nanoparticles; silver nanoparticles
Co-reporter:Kui Zhu, Jianzhong Shen, Richard Dietrich, Andrea Didier, Xingyu Jiang and Erwin Märtlbauer
Chemical Communications 2014 vol. 50(Issue 6) pp:676-678
Publication Date(Web):05 Nov 2013
DOI:10.1039/C3CC48100J
A cellular logic system capable of combinatorial and sequential logic operations based on bacterial protein-triggered cytotoxicity was constructed. Advanced devices such as a keypad lock, half-adder and several basic Boolean properties were demonstrated on the cells.
Co-reporter:Jidong Wang, Wenwen Chen, Jiashu Sun, Chao Liu, Qifang Yin, Lu Zhang, Yunlei Xianyu, Xinghua Shi, Guoqing Hu and Xingyu Jiang
Lab on a Chip 2014 vol. 14(Issue 10) pp:1673-1677
Publication Date(Web):17 Feb 2014
DOI:10.1039/C4LC00080C
This report describes a straightforward but robust tubing method for connecting polydimethylsiloxane (PDMS) microfluidic devices to external equipment. The interconnection is irreversible and can sustain a pressure of up to 4.5 MPa that is characterized experimentally and theoretically. To demonstrate applications of this high-pressure tubing technique, we fabricate a semicircular microfluidic channel to implement a high-throughput, size-controlled synthesis of poly(lactic-co-glycolic acid) (PLGA) nanoparticles ranging from 55 to 135 nm in diameter. This microfluidic device allows for a total flow rate of 410 mL h−1, resulting in enhanced convective mixing which can be utilized to precipitate small size nanoparticles with a good dispersion. We expect that this tubing technique would be widely used in microfluidic chips for nanoparticle synthesis, cell manipulation, and potentially nanofluidic applications.
Co-reporter:Lu Zhang, Yi Zhang, Chunyan Wang, Qiang Feng, Fei Fan, Guojun Zhang, Xixiong Kang, Xuzhen Qin, Jiashu Sun, Yinghui Li, and Xingyu Jiang
Analytical Chemistry 2014 Volume 86(Issue 20) pp:10461
Publication Date(Web):September 21, 2014
DOI:10.1021/ac503072a
This work develops an integrated microcapillary-based loop-mediated isothermal amplification (icLAMP) containing preloaded reagents and DNA extraction card, allowing for sample-to-answer screening of single nucleotide polymorphisms (SNPs) typing of the CYP2C19 gene from untreated blood samples with minimal user operation. With all reagents and the DNA extraction card preloaded inside the capillary, this icLAMP system can achieve on-site pretreatment, extraction, amplification, and detection of nucleic acids within 150 min, without the requirement for advanced instruments. As icLAMP technology carries many advantages such as disposability, easy operation, low cost, and reduced cross contamination and biohazard risks, we expect this system to have a great impact on point-of-care (POC) nucleic acid detection.
Co-reporter:Yi Zhang, Lu Zhang, Jiashu Sun, Yulei Liu, Xingjie Ma, Shangjin Cui, Liying Ma, Jianzhong Jeff Xi, and Xingyu Jiang
Analytical Chemistry 2014 Volume 86(Issue 14) pp:7057
Publication Date(Web):June 17, 2014
DOI:10.1021/ac5014332
This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA targets of human immunodeficiency virus (HIV) from multiple plasma samples, and achieves a high sensitivity of two copies of standard plasmid. As few nucleic acid detection methods can be wholly independent of external power supply and equipment, our cLAMP holds great promise for point-of-care applications in resource-poor settings.
Co-reporter:Yongming Guo, Yi Zhang, Huawu Shao, Zhuo Wang, Xuefei Wang, and Xingyu Jiang
Analytical Chemistry 2014 Volume 86(Issue 17) pp:8530
Publication Date(Web):August 13, 2014
DOI:10.1021/ac502461r
A simple and label-free colorimetric method for cadmium ions (Cd2+) detection using unmodified gold nanoparticles (AuNPs) is reported. The unmodified AuNPs easily aggregate in a high concentration of NaCl solution, but the presence of glutathione (GSH) can prevent the salt-induced aggregation of AuNPs. When Cd2+ is added to the stable mixture of AuNPs, GSH, and NaCl, Cd2+ can coordinate with 4× GSH as a spherical shaped complex, which decreases the amount of free GSH on the surface of gold nanoparticles to weaken the stability of AuNPs, and AuNPs will easily aggregate in high-salt conditions. On the basis of the mechanism, we design a simple, label-free colorimetric method using AuNPs accompanied by GSH in a high-salt environment to detect Cd2+ in water and digested rice samples.
Co-reporter:Wen-Wen CHEN, Yong-Ming GUO, Wen-Shu ZHENG, Yun-Lei XIANYU, Zhuo WANG, Xing-Yu JIANG
Chinese Journal of Analytical Chemistry 2014 Volume 42(Issue 3) pp:307-314
Publication Date(Web):March 2014
DOI:10.1016/S1872-2040(13)60714-8
Biochemical analysis assays based on colorimetric methods using gold nanoparticles have many advantages such as high sensitivity, good selectivity, naked-eyes readout and complex instruments free. These methods have good prospects in applications. This review mainly focuses on colorimetric assays applying gold nanoparticles for biomolecules detection.The determination (colorimetric and fluorometric) of AChE is based on RB-AuNPs. The well-dispersed RB-AuNPs (red) are induced to aggregate (purple) via electrostatic interaction in the presence of thiocholine derived from the hydrolysis of ATC catalyzed by AChE in the CSF of transgenic mice, accompanied with the fluorescence recovery of RB (the color of the stars changed from gray to green).
Co-reporter:Yunlei Xianyu, Zhuo Wang, and Xingyu Jiang
ACS Nano 2014 Volume 8(Issue 12) pp:12741
Publication Date(Web):November 21, 2014
DOI:10.1021/nn505857g
Current techniques for plasmonic immunoassay often require the introduction and additional conjugation of enzyme, and thus cannot accommodate conventional immunoassay platforms. Herein, we develop a plasmonic nanosensor that well accommodates conventional immunoassays and dramatically improves their sensitivity and stability. This plasmonic nanosensor directly employs alkaline phosphatase-triggered click chemistry between azide/alkyne functionalized gold nanoparticles as the readout. This straightforward approach broadens the applicability of nanoparticle-based immunoassays and has great potential for applications in resource-constrained settings.Keywords: alkaline phosphatase; click chemistry; gold nanoparticles; naked-eye readout; plasmonic immunoassay;
Co-reporter:Bo Jiang;WenFu Zheng;Wei Zhang
Science China Chemistry 2014 Volume 57( Issue 3) pp:356-364
Publication Date(Web):2014 March
DOI:10.1007/s11426-013-4971-0
Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study. Introducing the identified key aspects, including tissue-tissue interfaces, spatiotemporal chemical gradients, and dynamic mechanical forces, of living organs into the microfluidic system, “organs-on-chips” display an unprecedented application potential in a lot of biological fields such as fundamental physiological and pathophysiological research, drug efficacy and toxicity testing, and clinical diagnosis. Here, we review the recent development of organs-on-chips and briefly discuss their future challenges.
Co-reporter:Dr. Yuyun Zhao;Chunjie Ye;Dr. Wenwen Liu; Rong Chen; Xingyu Jiang
Angewandte Chemie 2014 Volume 126( Issue 31) pp:8265-8269
Publication Date(Web):
DOI:10.1002/ange.201401035
Abstract
We show that bimetallic nanoparticles (NPs) of AuPt without any surface modification are potent antibiotic reagents, while pure Au NPs or pure Pt NPs display no antibiotic activities. The most potent antibacterial AuPt NPs happen to be the most effective catalysts for chemical transformations. The mechanism of antibiotic action includes the dissipation of membrane potential and the elevation of adenosine triphosphate (ATP) levels. These bimetallic NPs are unique in that they do not produce reactive oxygen species as most antibiotics do. Being non-toxic to human cells, these bimetallic noble NPs might open an entry to a new class of antibiotics.
Co-reporter:Dr. Yuyun Zhao;Chunjie Ye;Dr. Wenwen Liu; Rong Chen; Xingyu Jiang
Angewandte Chemie 2014 Volume 126( Issue 31) pp:
Publication Date(Web):
DOI:10.1002/ange.201401156
Co-reporter:Wenwen Chen; Qizhai Li;Wenshu Zheng;Fang Hu; Guanxin Zhang; Zhuo Wang; Deqing Zhang; Xingyu Jiang
Angewandte Chemie International Edition 2014 Volume 53( Issue 50) pp:13734-13739
Publication Date(Web):
DOI:10.1002/anie.201407606
Abstract
We report a method for the rapid and efficient identification of bacteria making use of five probes having fluorescent characteristics (F-array) and subsequent statistical analysis. Eight kinds of bacteria, including normal and multidrug-resistant bacteria, are differentiated successfully. Our easy-to-perform and time-saving method consists of mixing bacteria and probes, recording fluorescent intensity data by automated flow cytometry, and statistical analysis. No washing steps are required in order to identify the different bacteria simultaneously.
Co-reporter:Dr. Yuyun Zhao;Chunjie Ye;Dr. Wenwen Liu; Rong Chen; Xingyu Jiang
Angewandte Chemie International Edition 2014 Volume 53( Issue 31) pp:
Publication Date(Web):
DOI:10.1002/anie.201401156
Co-reporter:Dr. Yuyun Zhao;Chunjie Ye;Dr. Wenwen Liu; Rong Chen; Xingyu Jiang
Angewandte Chemie International Edition 2014 Volume 53( Issue 31) pp:8127-8131
Publication Date(Web):
DOI:10.1002/anie.201401035
Abstract
We show that bimetallic nanoparticles (NPs) of AuPt without any surface modification are potent antibiotic reagents, while pure Au NPs or pure Pt NPs display no antibiotic activities. The most potent antibacterial AuPt NPs happen to be the most effective catalysts for chemical transformations. The mechanism of antibiotic action includes the dissipation of membrane potential and the elevation of adenosine triphosphate (ATP) levels. These bimetallic NPs are unique in that they do not produce reactive oxygen species as most antibiotics do. Being non-toxic to human cells, these bimetallic noble NPs might open an entry to a new class of antibiotics.
Co-reporter:Wenfu Zheng, Xingyu Jiang
Colloids and Surfaces B: Biointerfaces 2014 Volume 124() pp:97-110
Publication Date(Web):1 December 2014
DOI:10.1016/j.colsurfb.2014.08.026
•We reviewed the method to manipulate cell adhesion on surface.•We reviewed the manipulation of cell migration on surface.•We summarized the influence of physical cues of surfaces on differentiation of cells.•We summarized the cell–cell interactions on surface/interface.•The construction of in vitro model of chips: organ on chips.The use of micro/nanotechnology has become an indispensable strategy to manipulating cell microenvironments. By employing key elements of soft lithographical technologies including self-assembled monolayers (SAMs), microcontact printing (μCP), and microfluidic pattering (μFP) and a number of switchable surfaces such as electrochemical active, photosensitive, and thermosensitive surfaces, scientists can control the adhesion, proliferation, migration and differentiation of cells. By combining essential in vivo conditions, various physical or pathological processes such as cell–cell interaction in wound healing and tumor metastasis could be studied on well-defined surfaces and interfaces. By integrating key elements in live tissues, in vitro models mimicking basic structure and function of vital organs such as lung, heart, blood vessel, liver, kidney, and brain have been developed and greatly increased our knowledge of these important life processes. In this review, we will focus on the recent development of these interfacial methods and their application in fundamental biology research.
Co-reporter:Yi Zhang;Yongming Guo;Yunlei Xianyu;Wenwen Chen;Yuyun Zhao
Advanced Materials 2013 Volume 25( Issue 28) pp:3802-3819
Publication Date(Web):
DOI:10.1002/adma.201301334
Abstract
The advances of nanomaterials have provided exciting technologies and novel materials for protein detection, based on the unique properties associated with nanoscale phenomena such as plasmon resonance, catalysis and energy transfer. This article reviews a series of nanomaterials including nanoparticles, nanofibers, nanowires, and nanosheets, and evaluates their performances in the application for protein detection, focusing on approaches that realize ultrasensitive detection. Many of these nanomaterials were used to analyze clinically relevant protein biomarkers. Their detection in the picomolar, femtomolar or even zeptomolar regime has been realized, sometimes even with naked-eye readout. We summarize the detection methods and results according to materials and targets, review the current challenges, and discuss the solution in the context of technological integration such as combining nanomaterials with microfluidics, and classical analytical technologies.
Co-reporter:Yuyun Zhao ; Zeliang Chen ; Yanfen Chen ; Jie Xu ; Jinghong Li
Journal of the American Chemical Society 2013 Volume 135(Issue 35) pp:12940-12943
Publication Date(Web):August 19, 2013
DOI:10.1021/ja4058635
Co-presenting non-antibiotic drugs and pyrimidinethiol on gold nanoparticles (NPs) can generate broad-spectrum antibacterial and bactericidal activities against superbugs. Dimethylbiguanide (metformin), an anti-hyperglycemic drug, shows the best enhanced activity via increasing the ability to compromise bacterial cell walls. Synergistic effects are also reflected in the eradicating biofilm cells. Our findings suggest a large chemical space to develop new antibacterial materials to treat superbugs.
Co-reporter:Peiyuan Gong;Wenfu Zheng;Zhuo Huang;Wei Zhang;Dan Xiao
Advanced Functional Materials 2013 Volume 23( Issue 1) pp:42-46
Publication Date(Web):
DOI:10.1002/adfm.201201275
Abstract
Differentiated cells make up tissues and organs, and communicate within a complex, three dimensional (3D) environment. The spatial arrangement of cellular interactions is difficult to recapitulate in vitro. Here, a simple and rapid method for stepwise formation of 2D multicellular structures through the biotin-streptavidin (SA) interaction and further construction of controlled, 3D, multilayered, tissue-like structures by using the stress-induced rolling membrane (SIRM) technique is reported. The biotinylated cells connect with the SA-coated adherent cells to form a bilayer. The bilayer of two types of cells on the SIRM is transformed into 3D tubes, in which two types of cells can directly interact and communicate with each other, mimicking the in vivo conditions of tubular structures such as blood vessel. This method has the potential to recapitulate functional tubular structures for tissue engineering.
Co-reporter:Zhuo Huang and Xingyu Jiang
Journal of Materials Chemistry A 2013 vol. 1(Issue 46) pp:7652-7662
Publication Date(Web):14 Aug 2013
DOI:10.1039/C3TC31165A
In recent years, a number of new materials and techniques at the micro/nano-scale for neuroscience have been reported, in particular in studies of neuronal development and electronic addressing. They offer new capabilities to fabricate tools in controlling the surface properties (such as topography and chemistry) as well as executing electrical stimulations and measurements. Here we review the basic principles of these micro/nano-scale materials and highlight the important advances in this field, and finally provide some perspectives for the future.
Co-reporter:Yunlei Xianyu, Jiashu Sun, Yixuan Li, Yue Tian, Zhuo Wang and Xingyu Jiang
Nanoscale 2013 vol. 5(Issue 14) pp:6303-6306
Publication Date(Web):08 May 2013
DOI:10.1039/C3NR01697H
This report demonstrates a colorimetric, non-enzymatic glucose assay with a low detection limit of 0.07 μM based on negatively charged gold nanorod-enhanced redox reaction. This glucose assay could generate silver nanoparticles as the readout that can be visualized by the naked eye, and only 4 femtomoles of nanorods are needed for glucose determination in one human plasma sample.
Co-reporter:Yuyun Zhao and Xingyu Jiang
Nanoscale 2013 vol. 5(Issue 18) pp:8340-8350
Publication Date(Web):30 May 2013
DOI:10.1039/C3NR01990J
Widespread antibiotic resistance calls for new strategies. Nanotechnology provides a chance to overcome antibiotic resistance by multiple antibiotic mechanisms. This paper reviews the progress in activating gold nanoparticles with nonantibiotic or antibiotic molecules to combat bacterial resistance, analyzes the gap between experimental achievements and real clinical application, and suggests some potential directions in developing antibacterial nanodrugs.
Co-reporter:Jiashu Sun, Yunlei Xianyu, Mengmeng Li, Wenwen Liu, Lu Zhang, Dingbin Liu, Chao Liu, Guoqing Hu and Xingyu Jiang
Nanoscale 2013 vol. 5(Issue 12) pp:5262-5265
Publication Date(Web):12 Apr 2013
DOI:10.1039/C3NR01289A
This report demonstrates a microfluidic origami chip to synthesize monodisperse, doxorubicin-loaded poly(lactic-co-glycolic acid) nanoparticles with diameters of ∼100 nm, a size optimized for cellular uptake and anticancer efficacy, but difficult to achieve with existing approaches. This three-dimensional design in a microchannel may allow for the fabrication of polymeric nanoparticles in this size regime with ease.
Co-reporter:Mengmeng Li, Wenwen Liu, Jiashu Sun, Yunlei Xianyu, Jidong Wang, Wei Zhang, Wenfu Zheng, Deyong Huang, Shiyu Di, Yun-Ze Long, and Xingyu Jiang
ACS Applied Materials & Interfaces 2013 Volume 5(Issue 13) pp:5921
Publication Date(Web):June 22, 2013
DOI:10.1021/am401937m
In this work, we fabricated polymeric fibrous scaffolds for bone tissue engineering using primary human osteoblasts (HOB) as the model cell. By employing one simple approach, electrospinning, we produced poly(lactic-co-glycolic acid) (PLGA) scaffolds with different topographies including microspheres, beaded fibers, and uniform fibers, as well as the PLGA/nanohydroxyapatite (nano-HA) composite scaffold. The bone-bonding ability of electrospun scaffolds was investigated by using simulated body fluid (SBF) solution, and the nano-HA in PLGA/nano-HA composite scaffold can significantly enhance the formation of the bonelike apatites. Furthermore, we carried out in vitro experiments to test the performance of electrospun scaffolds by utilizing both mouse preosteoblast cell line (MC 3T3 E1) and HOB. Results including cell viability, alkaline phosphatase (ALP) activity, and osteocalcin concentration demonstrated that the PLGA/nano-HA fibers can promote the proliferation of HOB efficiently, indicating that it is a promising scaffold for human bone repair.Keywords: biodegradable scaffold; electrospinning; human bone repair; hydroxyapatite; microstructure;
Co-reporter:Yunlei Xianyu, Kui Zhu, Wenwen Chen, Xuefei Wang, Hongmei Zhao, Jiashu Sun, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2013 Volume 85(Issue 15) pp:7029
Publication Date(Web):July 24, 2013
DOI:10.1021/ac401925j
We report an ultrasensitive and colorimetric assay for Cu(II) via enzymatic amplification strategy. The enzymatic activity of horseradish peroxidase (HRP) is strongly inhibited by Cu(I), which can be used indirectly to assay Cu(II). The limit of detection (LOD) is 0.37 nM, and the detection of 20 nM Cu(II) in solution can be achieved with naked eyes. This assay can be used to construct a colorimetric logic gate.
Co-reporter:Wenfu Zheng;Wei Zhang
Advanced Healthcare Materials 2013 Volume 2( Issue 1) pp:95-108
Publication Date(Web):
DOI:10.1002/adhm.201200104
Abstract
The adhesion of cells on an extracellular matrix (ECM) (in vivo) or the surfaces of materials (in vitro) is a prerequisite for most cells to survive. The rapid growth of nano/microfabrication and biomaterial technologies has provided new materials with excellent surfaces with specific, desirable biological interactions with their surroundings. On one hand, the chemical and physical properties of material surfaces exert an extensive influence on cell adhesion, proliferation, migration, and differentiation. On the other hand, material surfaces are useful for fundamental cell biology research and tissue engineering. In this Review, an overview will be given of the chemical and physical properties of newly developed material surfaces and their biological effects, as well as soft lithographic techniques and their applications in cell biology research. Recent advances in the manipulation of cell adhesion by the combination of surface chemistry and soft lithography will also be highlighted.
Co-reporter:Yongming Guo, Zhuo Wang, Huawu Shao, Xingyu Jiang
Carbon 2013 Volume 52() pp:583-589
Publication Date(Web):February 2013
DOI:10.1016/j.carbon.2012.10.028
We have developed a simple, one-step hydrothermal method for the synthesis of highly fluorescent carbon nanoparticles (F-CNPs) with a high quantum yield (68%) and good photostability. The method requires less reaction time and a lower reaction temperature as compared with the previous reported methods. The as-prepared F-CNPs exhibit excellent emission property and high stability, as well as excitation-independent emission behavior. Moreover, it is attractive that F-CNPs can be used as an effective fluorescent probe for the detection of mercury ions with good selectivity and sensitivity in an aqueous solution.
Co-reporter:Xuan Mu, Wenfu Zheng, Le Xiao, Wei Zhang and Xingyu Jiang
Lab on a Chip 2013 vol. 13(Issue 8) pp:1612-1618
Publication Date(Web):28 Jan 2013
DOI:10.1039/C3LC41342J
Engineering functional vascular networks in vitro is critical for tissue engineering and a variety of applications. There is still a general lack of straightforward approaches for recapitulating specific structures and functions of vasculature. This report describes a microfluidic method that utilizes fibrillogenesis of collagen and a liquid mold to engineer three-dimensional vascular networks in hydrogel. The well-controlled vascular network demonstrates both mechanical stability for perfusing solutions and biocompatibility for cell adhesion and coverage. This technique enables the mimicry of passive diffusion in a nephron one of the main routes transferring soluble organic molecules. This approach could be used for in vitro modelling of mass transfer-involved physiology in vasculature-rich tissues and organs for regeneration and drug screening.
Co-reporter:Wenwen Liu, Wenfu Zheng, Bo Yuan and Xingyu Jiang
Integrative Biology 2013 vol. 5(Issue 3) pp:617-623
Publication Date(Web):09 Jan 2013
DOI:10.1039/C3IB20198H
Axon fasciculation is essential for wiring the nervous system during development, but its regulation by guidance cues remains unknown. By using a micropatterned coculture system, we developed a diffused long-term gradient of the Slit protein secreted by cells transfected with the Slit gene. Using hippocampal neurons, we show here that the Slit gradient induces axon fasciculation and that the extent of fasciculation depends on the Slit concentration. This Slit-induced axon fasciculation was abolished by inhibitors of the ROCK–myosin II signaling pathway. Interestingly, the activity of myosin II regulated the level of axon fasciculation. In addition, we showed that neurons with a high level of axon fasciculation express fewer L1 cell adhesion molecules (L1CAMs), and that those with low level of axon fasciculation have more L1CAMs. We suggest that Slit induces axon fasciculation and regulates the ROCK–myosin II signaling pathway and the expression level of L1CAMs. This approach establishes a simple and stable axon guidance model in vitro, and may be broadly applicable for investigating long-term events such as axon fasciculation, neuron migration, and axon regeneration.
Co-reporter:Wenwen Liu, Shige Xing, Bo Yuan, Wenfu Zheng and Xingyu Jiang
Integrative Biology 2013 vol. 5(Issue 10) pp:1244-1252
Publication Date(Web):25 Jul 2013
DOI:10.1039/C3IB40131F
Axon branching enables neurons to contact with multiple targets and respond to their microenvironment. Owing to its importance in neuronal network formation, axon branching has been studied extensively during the past decades. The chemical properties of extracellular matrices have been proposed to regulate axonal development, but the effects of their density changes on axon branching are not well understood. Here, we demonstrate that both the sharp broadening of substrate geometry and the sharp change of laminin density stimulate axon branching by using microcontact printing (μCP) and microfluidic printing (μFP) techniques. We also found that the change of axon branching stimulated by laminin density depends on myosin II activity. The change of laminin density induces asymmetric extensions of filopodia on the growth cone, which is the precondition for axon branching. These previously unknown mechanisms of change of laminin density-stimulated axon branching may explain how the extracellular matrices regulate axon branching in vivo and facilitate the establishment of neuronal networks in vitro.
Co-reporter:Xiaona Hu;Yuyun Zhao;Zhijian Hu;Aditya Saran;Shuai Hou;Tao Wen
Nano Research 2013 Volume 6( Issue 11) pp:822-835
Publication Date(Web):2013 November
DOI:10.1007/s12274-013-0360-4
Co-reporter:Guanglei Fu, Wenwen Chen, Xiuli Yue, Xingyu Jiang
Talanta 2013 Volume 103() pp:110-115
Publication Date(Web):15 January 2013
DOI:10.1016/j.talanta.2012.10.016
Co-reporter:Dingbin Liu, Wenwen Chen, Jinhua Wei, Xuebing Li, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2012 Volume 84(Issue 9) pp:4185
Publication Date(Web):April 5, 2012
DOI:10.1021/ac300545p
This report presents a highly sensitive, rhodamine B-covered gold nanoparticle (RB-AuNP) -based assay with dual readouts (colorimetric and fluorometric) for detecting organophosphorus and carbamate pesticides in complex solutions. The detection mechanism is based on the fact that these pesticides can inhibit the activity of acetylcholinesterase (AChE), thus preventing the generation of thiocholine (which turns the RB-AuNP solutions blue and unquenches the fluorescence of RB simultaneously). The color of the RB-AuNP solution remains red and the fluorescence of RB remains quenched. By use of this dual-readout assay, the lowest detectable concentrations for several kinds of pesticides including carbaryl, diazinon, malathion, and phorate were measured to be 0.1, 0.1, 0.3, and 1 μg/L, respectively, all of which are much lower than the maximum residue limits (MRL) as reported in the European Union pesticides database as well as those from the U.S. Department Agriculture (USDA). This assay allows detection of pesticides in real samples such as agricultural products and river water. The results in detecting pesticide residues collected from food samples via this method agree well with those from high-performance liquid chromatography (HPLC). This simple assay is therefore suitable for sensing pesticides in complex samples, especially in combination with other portable platforms.
Co-reporter:Kui Zhu, Yi Zhang, Sha He, Wenwen Chen, Jianzhong Shen, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2012 Volume 84(Issue 10) pp:4267
Publication Date(Web):April 27, 2012
DOI:10.1021/ac3010567
This letter presents a click-chemistry-based assay for proteins (CAP) that allows quantitative determination of the concentration of proteins, using azide- and alkyne-functionalized gold nanoparticles (AuNPs). Compared with conventional methods, CAP has a broader linear range for detection of proteins with good selectivity. CAP enables the analysis of total proteins in various sera and milk samples.
Co-reporter:Dingbin Liu;Wenwen Chen;Yue Tian;Sha He;Wenfu Zheng;Jiashu Sun;Zhuo Wang
Advanced Healthcare Materials 2012 Volume 1( Issue 1) pp:90-95
Publication Date(Web):
DOI:10.1002/adhm.201100002
Co-reporter:Wenfu Zheng, Bo Jiang, Dong Wang, Wei Zhang, Zhuo Wang and Xingyu Jiang
Lab on a Chip 2012 vol. 12(Issue 18) pp:3441-3450
Publication Date(Web):30 May 2012
DOI:10.1039/C2LC40173H
This microfluidic flow-stretch chip integrates fluid shear stress (FSS) and cyclic stretch (CS), two major mechanical stimulations in cardiovascular systems, for cultured cells. The model chip can deliver FSS and CS simultaneously or independently to vascular cells to mimic the haemodynamic microenvironment of blood vessels in vivo. By imposing FSS-only, CS-only, and FSS+CS stimulation on rat mesenchymal stem cells and human umbilical vein endothelial cells, we found the alignment of the cellular stress fibers varied with cell type and the type of stimulation. The flow-stretch chip is a reliable tool for simulating the haemodynamic microenvironment.
Co-reporter:Jiashu Sun, Mengmeng Li, Chao Liu, Yi Zhang, Dingbin Liu, Wenwen Liu, Guoqing Hu and Xingyu Jiang
Lab on a Chip 2012 vol. 12(Issue 20) pp:3952-3960
Publication Date(Web):04 Jul 2012
DOI:10.1039/C2LC40679A
This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. A numerical model is developed to simulate the Dean flow inside the curved geometry and to track the particle/cell trajectories, which is validated against the experimental observations and serves as a theoretical foundation for optimizing the operating conditions. Results from separating tumor cells (MCF-7 and Hela) spiked into whole blood indicate that 92.28% of blood cells and 96.77% of tumor cells are collected at the inner and the middle outlet, respectively, with 88.5% tumor recovery rate at a throughput of 3.33 × 107 cells min−1. We expect that this label-free microfluidic platform, driven by purely hydrodynamic forces, would have an impact on fundamental and clinical studies of circulating tumor cells.
Co-reporter:Yi Zhang, Yunfang Tang, Yi-Heui Hsieh, Chuen-Yuan Hsu, Jianzhong Xi, Kuan-Jiuh Lin and Xingyu Jiang
Lab on a Chip 2012 vol. 12(Issue 17) pp:3012-3015
Publication Date(Web):31 May 2012
DOI:10.1039/C2LC40590C
This work reports an integrated platform combining localized-surface plasmon resonance (LSPR) and microfluidic chips to carry out multiplexed and label-free protein analysis. We fabricated an optical bench to enable detection using only a laboratory UV-Vis spectrophotometer. This assay not only improves throughput, but also allows quantitative analysis.
Co-reporter:Qilong Zhao;Shiwen Wang;Yunyan Xie;Wenfu Zheng;Zhuo Wang;Le Xiao;Wei Zhang
Advanced Healthcare Materials 2012 Volume 1( Issue 5) pp:560-566
Publication Date(Web):
DOI:10.1002/adhm.201200008
Abstract
This report demonstrates an in vitro method for screening wound dressing candidates that can minimize the use of animals for developing better methods for wound care. The development of materials and formulations for wound dressings, an important application of biomaterials, is laboriously and ethically challenging because of the use of a large number of animals. A method for rapid and effective screening of wound dressings in vitro, therefore, is in great need. A cell-on-a-chip model was used to simulate the cutaneous wound in vitro and screen the performances of several electrospun fibrous wound dressings in enhancing wound healing. For comparison, the performances of wound dressings were also evaluated in a rat model. It was found that the results acquired by microchip model corroborates well with animal experiments. It is the first time, as far as we know, that a good correlation between in vitro and in vivo results is reported for fibrous wound dressings. The cell-on-a-chip wound model we developed here may change the way that scientists screen candidates for wound dressings.
Co-reporter:Yongming Guo, Zhuo Wang, Huawu Shao and Xingyu Jiang
Analyst 2012 vol. 137(Issue 2) pp:301-304
Publication Date(Web):03 Nov 2011
DOI:10.1039/C1AN15877E
A simple and one-pot method for the synthesis of water-soluble, red-emitting, highly fluorescent gold nanoparticles has been reported using 11-mercaptoundecanoic acid (11-MUA) as the protecting group. We found that the fluorescent gold nanoparticles could selectively detect copper ions in aqueous solution, with a limit of detection of about 87 nM.
Co-reporter:Zhenling Chen, Yi Dai, Zhe Dong, Menghui Li, Xuan Mu, Rui Zhang, Zhuo Wang, Wei Zhang, Jinghe Lang, Jinhua Leng and Xingyu Jiang
Integrative Biology 2012 vol. 4(Issue 9) pp:1090-1095
Publication Date(Web):06 Jul 2012
DOI:10.1039/C2IB00172A
This paper demonstrates an in vitro model to simulate the microenvironment of endometriosis. We used microfluidic channels with cover slips to pattern and release endometrial stromal cells (ESCs) and human peritoneal mesothelial cells (HPMCs) in a way that mimicked the pathophysiology of peritoneal endometriosis. This approach enabled observation in real time interactions between ESCs and HPMCs both in their normal and pathological states. HPMCs from control individuals were able to resist the invasion of ESCs from both control and endometriotic individuals. By contrast, HPMCs from endometriotic individuals were unable to resist the invasion of ESCs from both normal and endometriotic individuals. We further analyzed the dynamics between HPMCs and ESCs from endometriotic individuals. HPMCs from endometriotic individuals relaxed their adhesion to each other at the beginning of invasion of ESCs, lose their adhesion to the substrate and apoptosed when surrounded by ESCs. These data implicate that the peritoneal physiology may play an important role in endometriosis.
Co-reporter:Wenfu Zheng, Yunyan Xie, Wei Zhang, Dong Wang, Wanshun Ma, Zhuo Wang and Xingyu Jiang
Integrative Biology 2012 vol. 4(Issue 9) pp:1102-1111
Publication Date(Web):14 Jun 2012
DOI:10.1039/C2IB20094E
Mesenchymal stem cells (MSCs), the multipotent progenitor cells, are sensitive to fluid shear stress (FSS). MSCs can migrate through the blood stream by intravasation into the circulatory system to transfer to distant positions through the blood stream. During the transferring process, MSCs may differentiate into cells of corresponding tissues for repair, or remain undifferentiated and initiate ectopic tissue formation, lipid accumulation, or calcification, which are closely related to the pathology of atherosclerosis. However, how the MSCs sense and respond to vascular FSS stimulation and lead to subsequent biological effects remains elusive. In this study, by using an in situ time-lapse microfluidic cell culture and observation system, we found that rat mesenchymal stem cells (rMSCs) presented a contraction and re-spread (CRS) process when they were initially subjected to a physiological FSS (1.3 Pa). Our subsequent studies demonstrated that integrin and cilia played key roles in sensing FSS. Calcium, F-actin, and Rho-kinase were key molecules in the mechanotransduction of the CRS of the rMSCs. Our study revealed the immediate response of the rMSCs to FSS. It will be helpful for the understanding of MSC-related tissue repair and the role of MSCs in the initiation of atherosclerosis.
Co-reporter:Yingyi Liu;Jie Yu;Wenwen Chen;Dingbin Liu;Zhuo Wang
Chinese Journal of Chemistry 2012 Volume 30( Issue 9) pp:2047-2051
Publication Date(Web):
DOI:10.1002/cjoc.201200655
Abstract
We have developed a microfluidic chip for colorimetric Cu2+ detection. In this chip, it is facile to do colorimetric Cu2+ detection based on gold nanoparticles. This method has a dynamic detection range from 0.75 to 50 µmol/L with only 20 µL solution including detection reagents and sample. The result can be readout by naked eye and photographed by digital cameras. With the help of image processing software, we could measure the RGB value and calculate the Blue/Red ratio for more accurate quantification. Tap water could be detected in this portable chip.
Co-reporter:Yi Zhang, Xuwei Wang, Lusheng Song, Chuanlai Xu, Liying Ma, Zhanhua Li, Jianzhong Xi and Xingyu Jiang
Analytical Methods 2012 vol. 4(Issue 10) pp:3466-3470
Publication Date(Web):19 Jul 2012
DOI:10.1039/C2AY25485A
High-throughput assays necessitate high-throughput data analysis. Arrayed microfluidic immunoassay shows the capability of high-throughput protein detection. However, its development was restricted by the low efficiency of downstream data analysis. We present herein programming-based image processing through the local recognition of a sub-array followed by the region-growing algorithm to achieve fast, convenient and precise extraction of information with reduced personal bias.
Co-reporter:Yingyi Liu;Jie Yu;Meihong Du;Wenjun Wang;Wei Zhang
Biomedical Microdevices 2012 Volume 14( Issue 1) pp:17-23
Publication Date(Web):2012 February
DOI:10.1007/s10544-011-9581-z
This paper describes a vacuum-accelerated microfluidic immunoassay (we abbreviate it as VAMI) by sandwiching a filter membrane between a two-layer chip. A direct assay of IgG demonstrated that VAMI could simultaneously achieve higher sensitivity and require less time compared with conventional microfluidic immunoassays. We further applied VAMI to carry out a 3-step competitive assay (including antigen immobilization, competitive reaction and 2nd antibody reaction) for detecting the illegal food additive Sudan Red. A total assay time of 15 min with a limit of detection (LOD) of 1 ng ml-1 is achieved.
Co-reporter:PeiYuan Gong;Wen Zheng;Dan Xiao
Science China Life Sciences 2012 Volume 55( Issue 10) pp:862-871
Publication Date(Web):2012 October
DOI:10.1007/s11427-012-4385-9
Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) environment. The in vitro recapitulation of tissue-like structures is meaningful, not only for fundamental cell biology research, but also for tissue engineering (TE). Currently, TE research adopts either the top-down or bottom-up approach. The top-down approach involves defining the macroscopic tissue features using biomaterial scaffolds and seeding cells into these scaffolds. Conversely, the bottom-up approach aims at crafting small tissue building blocks with precision-engineered structural and functional microscale features, using physical and/or chemical approaches. The bottom-up strategy takes advantage of the repeating structural and functional units that facilitate cell-cell interactions and cultures multiple cells together as a functional unit of tissue. In this review, we focus on currently available microscale methods that can control mammalian cells to assemble into 3D tissue-like structures.
Co-reporter:Lusheng Song;Yi Zhang;Wenjun Wang;Liying Ma;Yong Liu
Biomedical Microdevices 2012 Volume 14( Issue 4) pp:631-640
Publication Date(Web):2012 August
DOI:10.1007/s10544-012-9644-9
The essential step for HIV spreading limitation is the screening tests. However, there are multiple disadvantages in current screening assays which need further confirmation test. Herein we developed a rapid HIV assay combining screening and confirmation test by using the microfluidic network assay. Meanwhile, the assay is accelerated by bypassing the step of blocking. We call this method as microfluidic assay without blocking (MAWB). Both the limit of detection and reagent incubation time of MAWB are determined by screening of one model protein pair: ovalbumin and its antibody. The assay time is accelerated about 25% while the limit of detection (LOD) is well kept. Formatting the method in for both HIV screening (testing 8 HIV-related samples) and confirmation (assaying 6 kinds of HIV antibodies of each sample) within 30 min was successful. Fast HIV screening and confirmation of 20 plasma samples were also demonstrated by this method. MAWB improved the assay speed while keeping the LOD of conventional ELISA. Meanwhile, both the accuracy and throughput of MAWB were well improved, which made it an excellent candidate for a quick HIV test for both screening and confirmation. Methods like this one will find wide applications in clinical diagnosis and biochemical analysis based on the interactions between pairs of molecules.
Co-reporter:Dingbin Liu, Shouju Wang, Magdalena Swierczewska, Xinglu Huang, Ashwinkumar A. Bhirde, Jiashu Sun, Zhuo Wang, Min Yang, Xingyu Jiang, and Xiaoyuan Chen
ACS Nano 2012 Volume 6(Issue 12) pp:10999
Publication Date(Web):November 2, 2012
DOI:10.1021/nn3046192
We designed a recyclable Hg2+ probe based on Rhodamine B isothiocyanate (RBITC)-poly(ethylene glycol) (PEG)-comodified gold nanoparticles (AuNPs) with excellent robustness, selectivity, and sensitivity. On the basis of a rational design, only Hg2+ can displace RBITC from the AuNP surfaces, resulting in a remarkable enhancement of RBITC fluorescence initially quenched by AuNPs. To maintain stability and monodispersity of AuNPs in real samples, thiol-terminated PEG was employed to bind with the remaining active sites of AuNPs. Besides, this displacement assay can be regenerated by resupplying free RBITC into the AuNPs solutions that were already used for detecting Hg2+. Importantly, the detection limit of this assay for Hg2+ (2.3 nM) was lower than the maximum limits guided by the United States Environmental Protection Agency as well as that permitted by the World Health Organization. The efficiency of this probe was demonstrated in monitoring Hg2+ in complex samples such as river water and living cells.Keywords: gold nanoparticles; recyclable detection; Rhodamine B isothiocyanate; selectivity; sensitivity
Co-reporter:Kang Sun, Yunyan Xie, Dekai Ye, Yuyun Zhao, Yan Cui, Fei Long, Wei Zhang, and Xingyu Jiang
Langmuir 2012 Volume 28(Issue 4) pp:2131-2136
Publication Date(Web):November 15, 2011
DOI:10.1021/la2041967
This Article introduces a simple method of cell patterning, inspired by the mussel anchoring protein. Polydopamine (PDA), artificial polymers made from self-polymerization of dopamine (a molecule that resembles mussel-adhesive proteins), has recently been studied for its ability to make modifications on surfaces in aqueous solutions. We explored the interfacial interaction between PDA and poly(ethylene glycol) (PEG) using microcontact printing (μCP). We patterned PDA on several substrates such as glass, polystyrene, and poly(dimethylsiloxane) and realized spatially defined anchoring of mammalian cells as well as bacteria. We applied our system in investigating the relationship between areas of mammalian nuclei and that of the cells. The combination of PDA and PEG enables us to make cell patterns on common laboratorial materials in a mild and convenient fashion.
Co-reporter:Yan Cui, Yuyun Zhao, Yue Tian, Wei Zhang, Xiaoying Lü, Xingyu Jiang
Biomaterials 2012 33(7) pp: 2327-2333
Publication Date(Web):
DOI:10.1016/j.biomaterials.2011.11.057
Co-reporter:Dingbin Liu, Zhuo Wang and Xingyu Jiang
Nanoscale 2011 vol. 3(Issue 4) pp:1421-1433
Publication Date(Web):28 Feb 2011
DOI:10.1039/C0NR00887G
In recent years, gold nanoparticles (AuNPs) have drawn considerable research attention in the fields of catalysis, drug delivery, imaging, diagnostics, therapy and biosensors due to their unique optical and electronic properties. In this review, we summarized recent advances in the development of AuNP-based colorimetric and fluorescent assays for ions including cations (such as Hg2+, Cu2+, Pb2+, As3+, Ca2+, Al3+, etc) and anions (such as NO2−, CN−, PF6−, F−, I−, oxoanions), and small organic molecules (such as cysteine, homocysteine, trinitrotoluene, melamine and cocaine, ATP, glucose, dopamine and so forth). Many of these species adversely affect human health and the environment. Moreover, we paid particular attention to AuNP-based colorimetric and fluorescent assays in practical applications.
Co-reporter:Mengmeng Li, Yun-Ze Long, Dayong Yang, Jiashu Sun, Hongxing Yin, Zhili Zhao, Wenhao Kong, Xingyu Jiang and Zhiyong Fan
Journal of Materials Chemistry A 2011 vol. 21(Issue 35) pp:13159-13162
Publication Date(Web):08 Aug 2011
DOI:10.1039/C1JM12240A
We report on a novel spinning technique, termed double-spinning (DS) by combining electro-spinning (ES) and centrifuge-spinning (CS), to fabricate superfine polymer fibers. With a low operating voltage and a slow rotating speed, DS can generate excellent aligned fibrous arrays and two-layer grids with different angles.
Co-reporter:Xueen Fang, Hui Chen, Shaoning Yu, Xingyu Jiang, and Jilie Kong
Analytical Chemistry 2011 Volume 83(Issue 3) pp:690
Publication Date(Web):December 10, 2010
DOI:10.1021/ac102858j
Multiplex gene assay is a valuable molecular tool not only in academic science but also in clinical diagnostics. Multiplex PCR assays, DNA microarrays, and various nanotechnology-based methods are examples of major techniques developed for analyzing multiple genes; none of these, however, are suitable for point-of-care diagnostics, especially in resource-limited settings. In this report, we describe an octopus-like multiplex microfluidic loop-mediated isothermal amplification (mμLAMP) assay for the rapid analysis of multiple genes in the point-of-care format and provide a robust approach for predicting viruses. This assay with the ability of analyzing multiple genes qualitatively and quantitatively is highly specific, operationally simple, and cost/time-effective with the detection limit of less than 10 copies/μL in 2 μL quantities of sample within 0.5 h. We successfully developed a mμLAMP chip for differentiating three human influenza A substrains and identifying eight important swine viruses.
Co-reporter:Xueen Fang, Hui Chen, Xingyu Jiang, and Jilie Kong
Analytical Chemistry 2011 Volume 83(Issue 9) pp:3596
Publication Date(Web):March 21, 2011
DOI:10.1021/ac200024a
In this report, we describe a simple-to-fabricate microfluidic device constructed on a silica gel plate by a waterproof marker pen. We call it pen-based assay on silica, which was successfully applied to simultaneously analyze multiple targets qualitatively and quantitatively based on colorimetric assays for protein, glucose, and pH value.
Co-reporter:Yunyan Xie, Wei Zhang, Liming Wang, Kang Sun, Yi Sun and Xingyu Jiang
Lab on a Chip 2011 vol. 11(Issue 17) pp:2819-2822
Publication Date(Web):21 Jul 2011
DOI:10.1039/C0LC00562B
Collective migration is critical to many physiological processes, but few methods allow for studying this behavior with precisely controlled cell–cell interaction. Here we report the development of a microchip based on co-culture of different types of cells and selective injury, and explore the dynamics of epithelial collective migration triggered by a real cell group.
Co-reporter:Tangsong Li, Kui Zhu, Sha He, Xi Xia, Shaoqin Liu, Zhuo Wang and Xingyu Jiang
Analyst 2011 vol. 136(Issue 14) pp:2893-2896
Publication Date(Web):24 May 2011
DOI:10.1039/C1AN15256D
We developed a simple, non-enzymatic approach for the colorimetric detection of glucose based on a gold nanoparticles (Au NPs) assisted silver mirror reaction (AuSMR). The linear range of the concentration of glucose is from 0.04 mM to 1 mM, and the lowest concentration that can be distinguished by the naked eye is 10 nM. This approach has been successfully used for detecting glucose in serum.
Co-reporter:Kang Sun, Bo Jiang, Xingyu Jiang
Journal of Electroanalytical Chemistry 2011 Volume 656(1–2) pp:223-230
Publication Date(Web):15 June 2011
DOI:10.1016/j.jelechem.2010.11.008
Electrochemical desorption (ECD) of self-assembled monolayers (SAMs) is an important process that can dynamically modify surface properties. ECD of SAMs has found applications in biochemical assays and the precise control of cell adhesion. As cell adhesion is controlled by the chemistry of underlying substrate, ECD provides a means to achieve spatiotemporal control of cell behaviors by electrically addressing the substrate. In this minireview, we first introduce the principles of ECD, followed by its applications in dynamically modifying chemistry and biochemistry at the intersurface. Finally, we highlight its applications in cell biology.
Co-reporter:Weisi Qu;Yingyi Liu;Dingbin Liu; Zhuo Wang; Xingyu Jiang
Angewandte Chemie International Edition 2011 Volume 50( Issue 15) pp:3442-3445
Publication Date(Web):
DOI:10.1002/anie.201006025
Co-reporter:Yongming Guo, Zhuo Wang, Weisi Qu, Huawu Shao, Xingyu Jiang
Biosensors and Bioelectronics 2011 Volume 26(Issue 10) pp:4064-4069
Publication Date(Web):15 June 2011
DOI:10.1016/j.bios.2011.03.033
A simple, cost-effective and rapid colorimetric method for any or all of Hg2+, Pb2+ and Cu2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg2+, Pb2+ and Cu2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg2+, Pb2+ and Cu2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200 nM Hg2+, Pb2+ or Cu2+ using 42 nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas.
Co-reporter:Kang Sun, Lusheng Song, Yunyan Xie, Dingbin Liu, Dong Wang, Zhuo Wang, Wanshun Ma, Jinsong Zhu, and Xingyu Jiang
Langmuir 2011 Volume 27(Issue 10) pp:5709-5712
Publication Date(Web):April 26, 2011
DOI:10.1021/la2012099
We report a one-step, mild method to modify antifouling oligo(ethylene glycol)-terminated self-assembled monolayers. We demonstrate for the first time that self-polymerized dopamine, previously reported as an underwater adhesive, can be patterned on typical antifouling surfaces by microfluidic patterning or microcontact printing. The patterns can be applied in spatiotemporal cell patterning.
Co-reporter:Dingbin Liu;Wenwen Chen;Kang Sun; Ke Deng; Wei Zhang; Zhuo Wang; Xingyu Jiang
Angewandte Chemie International Edition 2011 Volume 50( Issue 18) pp:4103-4107
Publication Date(Web):
DOI:10.1002/anie.201008198
Co-reporter:Yuyun Zhao ; Yue Tian ; Yan Cui ; Wenwen Liu ; Wanshun Ma
Journal of the American Chemical Society 2010 Volume 132(Issue 35) pp:12349-12356
Publication Date(Web):August 13, 2010
DOI:10.1021/ja1028843
This report illustrates a new strategy in designing antibacterial agents—a series of commercially available compounds, amino-substituted pyrimidines (themselves completely inactive as antibiotics), when presented on gold nanoparticles (NPs), show antibacterial activities against multidrug-resistant clinical isolates, without external sources of energy such as IR. These pyrimidine-capped gold NPs exert their antibiotic actions via sequestration of magnesium or calcium ions to disrupt the bacterial cell membrane, resulting in leakage of cytoplasmic contents including nucleic acids from compromised cell membranes, and via interaction with DNA and inhibition of protein synthesis by internalized NPs. These amino-substituted pyrimidine-capped gold NPs induce bacterial resistance more slowly compared with conventional, small-molecule antibiotics and appear harmless to human cells; these NPs may hence be useful for clinical applications.
Co-reporter:Bo Yuan;Yong Li;Dong Wang;Yunyan Xie;Yingyi Liu;Li Cui;Fuquan Tu;Hao Li;Hang Ji;Wei Zhang
Advanced Functional Materials 2010 Volume 20( Issue 21) pp:3715-3720
Publication Date(Web):
DOI:10.1002/adfm.201001298
Abstract
A novel method combining microfluidic channels and holey poly(dimethylsiloxane) PDMS membranes is presented for patterning multiple types of cells on a large variety of substrate, including substrate bearing micro- and nanometer-scale structures. Different kinds of cell are patterned on diverse flat substrates composed of various materials. Further, different cells are patterned on substrate with microgrooved substrate, and the influence of cell–cell and cell–substrate interactions about cell group behaviors is shown. This method provides a new approach for studying many processes in vitro caused by cell–cell and/or cell–substrate interactions. In addition, the method is straightforward, easy to access, convenient, and may be useful for a broad set of biological studies.
Co-reporter:Wei Chen, Sha He, Wenying Pan, Yu Jin, Wei Zhang, and Xingyu Jiang
Chemistry of Materials 2010 Volume 22(Issue 23) pp:6212
Publication Date(Web):November 3, 2010
DOI:10.1021/cm102129k
Co-reporter:Yuyun Zhao, Zhuo Wang, Wei Zhang and Xingyu Jiang
Nanoscale 2010 vol. 2(Issue 10) pp:2114-2119
Publication Date(Web):09 Aug 2010
DOI:10.1039/C0NR00309C
This report shows that, of the Tween series (Tween 20, Tween 40, Tween 60 and Tween 80) of nonionic surfactants adsorbed on gold nanoparticles (NPs), Tween 80 makes the NPs most stably dispersed in aqueous solutions with or without the presence of representative biological molecules, such as nucleic acids or proteins of different sizes, isoelectric points (pIs) and shapes. In addition, the stability of gold NPs already modified with poly(L-lysine)-graft-poly(ethylene glycol) (PLL-PEG) or hexa(ethylene glycol)-terminated undecanylthiol (HS(CH2)11EG6OH) is further improved in solutions of proteins when Tween 80 is co-adsorbed on the gold NPs. This strategy is the most effective when adsorption of Tween 80 on gold NPs precedes the coating of PLL-PEG or HS(CH2)11EG6OH on the NPs.
Co-reporter:Dingbin Liu, Weisi Qu, Wenwen Chen, Wei Zhang, Zhuo Wang, and Xingyu Jiang
Analytical Chemistry 2010 Volume 82(Issue 23) pp:9606
Publication Date(Web):November 11, 2010
DOI:10.1021/ac1021503
We provide a highly sensitive and selective assay to detect Hg2+ in aqueous solutions using gold nanoparticles modified with quaternary ammonium group-terminated thiols at room temperature. The mechanism is the abstraction of thiols by Hg2+ that led to the aggregation of nanoparticles. With the assistance of solar light irradiation, the detection limit can be as low as 30 nM, which satisfies the guideline concentration of Hg2+ in drinking water set by the WHO. In addition, the dynamic range of detection is wide (3 × 10−8−1 × 10−2 M). This range, to our best knowledge, is the widest one that has been reported so far in gold nanoparticle (AuNP)-based assays for Hg2+.
Co-reporter:Wenfu Zheng, Zhuo Wang, Wei Zhang and Xingyu Jiang
Lab on a Chip 2010 vol. 10(Issue 21) pp:2906-2910
Publication Date(Web):15 Sep 2010
DOI:10.1039/C005274D
This report shows methods to fabricate polydimethylsiloxane (PDMS) microfluidic systems for long-term (up to 10 day) cell culture. Undesired bubble accumulation in microfluidic channels abruptly changes the microenvironment of adherent cells and leads to the damage and death of cells. Existing bubble trapping approaches have drawbacks such as the need to pause fluid flow, requirement for external vacuum or pressure source, and possible cytotoxicity. This study reports two kinds of integrated bubble trap (IBT) which have excellent properties, including simplicity in structure, ease in fabrication, no interference with the flow, and long-term stability. IBT-A provides the simplest solution to prevent bubbles from entering microfluidic channels. In situ time-lapse imaging experiments indicate that IBT-B is an excellent device both for bubble trapping and debubbling in cell-loaded microfluidics. MC 3T3 E1 cells, cultured in a long and curved microfluidic channel equipped with IBT-B, showed high viability and active proliferation after 10 days of continuous fluid flow. The comprehensive measures taken in our experiments have led to successful long-term, bubble-free, on-chip culture of cells.
Co-reporter:Xueen Fang, Yingyi Liu, Jilie Kong and Xingyu Jiang
Analytical Chemistry 2010 Volume 82(Issue 7) pp:3002
Publication Date(Web):March 10, 2010
DOI:10.1021/ac1000652
This work shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during LAMP amplification) or via absorbance measured by an optic sensor; we call this system microLAMP (μLAMP). It is capable of analyzing target nucleic acids quantitatively with high sensitivity and specificity. The assay is straightforward in manipulation. It requires a sample volume of 0.4 μL and is complete within 1 h. The sensitivity of the assay is comparable to standard methods, where 10 fg of DNA sample could be detected under isothermal conditions (63 °C). A real time quantitative μLAMP assay using absorbance detection is possible by integration of optical fibers within the chip.
Co-reporter:Wenying Pan, Wei Chen and Xingyu Jiang
Analytical Chemistry 2010 Volume 82(Issue 10) pp:3974
Publication Date(Web):April 28, 2010
DOI:10.1021/ac1000493
We develop a novel method for Western blot based on microfluidics, incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol. Compared with the conventional method which could detect only one protein, the microfluidic Western blot could analyze at least 10 proteins simultaneously from a single sample, and it requires only about 1% of the amount of antibody used in conventional Western blot.
Co-reporter:Dong Wang, Yunyan Xie, Bo Yuan, Jiang Xu, Peiyuan Gong and Xingyu Jiang
Integrative Biology 2010 vol. 2(Issue 5-6) pp:288-293
Publication Date(Web):19 Mar 2010
DOI:10.1039/B920644B
We present a device for stretching cells adhering to elastic membranes in equiaxial or uniaxial mode, meanwhile allowing real-time imaging of molecular dynamics of live cells at high resolution on an inverted microscope during the entire process of the stretch. We obtained high-resolution images of stress fibers at each stage of the stretch, and found that stress fibers were shortened after one stretching cycle. We, for the first time, captured real-time images of the process of stress fiber disassembly during stretching. Several adjacent stress fibers appeared to reassemble into a single one after stretching. All these indicated that mechanical stretching played important roles in the rearrangement of actin filaments. This device will be especially useful in studies of the molecular dynamics in the process of mechanotransduction. The device is fabricated on a glass slide through a simple procedure and is adaptable to most ordinary laboratories.
Co-reporter:Yu Jin, Dayong Yang, Dongyang Kang and Xingyu Jiang
Langmuir 2010 Volume 26(Issue 2) pp:1186-1190
Publication Date(Web):August 18, 2009
DOI:10.1021/la902313t
We report a one-step method to fabricate necklace-like structures from zero-dimensional materials via electrospinning. PVA was used as polymer matrix for accomplishing necklace-like arrays of silica particles. We systemically investigated how the diameter of SiO2 particles, the weight ratio of PVA to SiO2, the voltage, and the relative content of PVA/SiO2/H2O influenced the morphology of electrospun fibers. SiO2 particles with diameter of 143 nm tended to aggregate into bunches in the fibers, while 265 and 910 nm SiO2 particles tended to align along the fibers one by one, resembling necklaces. The content of water in the PVA/SiO2/H2O solution showed critical influence on the diameter of fibers and consequently determined the morphology. Too thin solutions gave birth to blackberry-like structure; too condensed solution was too hard to eject from the orifice of the needle; when the ingredient was elaborately tailored, we obtained necklace-like structures. We believe that these results can serve as references to generating other complex structures involving polymers and particles via electrospinning and that these structures will be potentially useful in photoelectric devices, drug release, and optical components.
Co-reporter:Zhenling Chen, Wei Chen, Bo Yuan, Le Xiao, Dingbin Liu, Yu Jin, Baogang Quan, Jia-ou Wang, Kurash Ibrahim, Zhuo Wang, Wei Zhang, and Xingyu Jiang
Langmuir 2010 Volume 26(Issue 23) pp:17790-17794
Publication Date(Web):November 1, 2010
DOI:10.1021/la103132m
This report establishes an in vitro model on glass surfaces for patterning multiple types of cells to simulate cell−cell interactions in vivo. The model employs a microfluidic system and poly(ethylene glycol)-terminated oxysilane (PEG-oxysilane) to modify glass surfaces in order to resist cell adhesion. The system allows the selective confinement of different types of cells to realize complete confinement, partial confinement, and no confinement of three types of cells on glass surfaces. The model was applied to study intercellular interactions among human umbilical vein endothelial cells (HUVEC), PLA 801 C and PLA801 D cells.
Co-reporter:Yi Sun, Yingyi Liu, Weisi Qu, Xingyu Jiang
Analytica Chimica Acta 2009 Volume 650(Issue 1) pp:98-105
Publication Date(Web):14 September 2009
DOI:10.1016/j.aca.2009.05.018
Development of new tools catalyzes progress in biochemical sciences [G.M. Whitesides, E. Ostuni, S. Takayama, X.Y. Jiang, D.E. Ingber, Annual Review of Biomedical Engineering 3 (2001) 335]. Recent advances in micro-/nano-technology have resulted in an explosion of the number of new tools available for biochemical sciences. We have used surface chemistry, nano-structures and microfluidics to create a set of tools applicable for problems ranging from molecular to cellular analysis. These tools will promote the understanding of fundamental problems in cell biology, development and neurobiology, and become useful for real-world applications such as molecular diagnostics, food analysis and environmental monitoring.
Co-reporter:Wen-Wen LIU, Zhen-Ling CHEN, Xing-Yu JIANG
Chinese Journal of Analytical Chemistry 2009 Volume 37(Issue 7) pp:943-949
Publication Date(Web):July 2009
DOI:10.1016/S1872-2040(08)60113-9
The technologies that we call as cell micropatterning allow the control of the shape and size of cell adhesion. Combination of micro/nano technology, surface chemistry, electrochemistry, and photochemistry enables us to control the adhesion, migration, differentiation of cells, and the interactions between different types of cells. These methodologies bring about a new platform for the studies of cell biology. This article reviews a number of techniques for cell patterning and compares their advantages and disadvantages. It also reviews certain applications of cell micropatterning, including those for fundamental studies in cell biology, tissue engineering, and cell-based biosensors.
Co-reporter:Zhenling Chen Dr.;Yong Li;Wenwen Liu;Dongzhou Zhang;Yuyun Zhao;Bo Yuan
Angewandte Chemie International Edition 2009 Volume 48( Issue 44) pp:8303-8305
Publication Date(Web):
DOI:10.1002/anie.200902708
Co-reporter:Zhenling Chen Dr.;Yong Li;Wenwen Liu;Dongzhou Zhang;Yuyun Zhao;Bo Yuan
Angewandte Chemie 2009 Volume 121( Issue 44) pp:8453-8455
Publication Date(Web):
DOI:10.1002/ange.200902708
Co-reporter:Dingbin Liu;Yunyan Xie;Huawu Shao
Angewandte Chemie International Edition 2009 Volume 48( Issue 24) pp:4406-4408
Publication Date(Web):
DOI:10.1002/anie.200901130
Co-reporter:Dayong Yang, Xing Liu, Yu Jin, Ying Zhu, Dongdong Zeng, Xingyu Jiang and Hongwei Ma
Biomacromolecules 2009 Volume 10(Issue 12) pp:
Publication Date(Web):November 19, 2009
DOI:10.1021/bm900955p
Fabrication of poly(dimethylsiloxane) (PDMS)/poly(methyl methacrylate) (PMMA) nanofibers is critical to harness the advantage of nanostructured membrane applied in protein microarrays. Electrospinning (ES) of PDMS nanofibers is challenging because of the relatively low molecular weight of PDMS prepolymer. We report a strategy to fabricate PDMS/PMMA nanofibers via ES by introducing carrier polymer PMMA into PDMS solutions to supplement the deficiency of chain entanglements in the PDMS prepolymer. The prepared PDMS/PMMA nanofibrous membrane (PDMS/PMMA NFM) was successfully used as substrates for protein microarrays. The results of immunoassays showed the superior performance of PDMS/PMMA NFM as 3D substrate for protein microarrays; the limit-of-detection (LOD) on PDMS/PMMA NFM was 32 times lower than that on nitrocellulose membrane. The realization of ES PDMS extends the scope of ES materials from thermoplastic polymers to thermosetting materials. Given the simplicity, low cost, and high efficiency of ES technology, we believe that PDMS/PMMA NFM is a promising 3D substrate for protein microarrays.
Co-reporter:DaYong Yang;Yang Wang;DongZhou Zhang;YingYi Liu
Science Bulletin 2009 Volume 54( Issue 17) pp:2911-2917
Publication Date(Web):2009 September
DOI:10.1007/s11434-009-0241-0
Many of the applications proposed for bioassays, scaffolds for tissue engineering, filtrations, and supports for catalysts require polymeric membranes with large specific surface areas. Polycarbonate (PC) is a possible candidate for these applications because of its excellent mechanical performance and good biocompatibility. Electrospinning is a simple and effective method for large-scale fabrication of micro-/nano- fibrous membranes with large specific surface areas. How to control the morphology of electrospun PC fibers, however, has not been systematically investigated. We describe the controllable fabrication of continuous and uniform PC fibers. We electrospin PC/chloroform solutions doped with different types of surfactants including anionic, zwitterionic, nonionic and cationic surfactants. Only cationic surfactants can lead to the successful fabrication of uniform PC fibers. After the analysis of the correlation between solution properties such as viscosity, surface tension, and conductivity and the morphology of electrospun fibers, we conclude that the addition of cationic surfactants such as cetane trimethyl ammonium bromide (CTAB) that leads to a decrease in viscosity is the main factor responsible for the formation of PC fibers. The demonstration of the fabrication of uniform PC fibers will lend experience to processing other polymers into fibers via electrospinning.
Co-reporter:Dayong Yang;Xin Niu;Yingyi Liu;Yang Wang;Xuan Gu;Lusheng Song;Rui Zhao;Liying Ma;Yiming Shao
Advanced Materials 2008 Volume 20( Issue 24) pp:4770-4775
Publication Date(Web):
DOI:10.1002/adma.200801302
Co-reporter:Kang Sun, Zongxing Wang and Xingyu Jiang
Lab on a Chip 2008 vol. 8(Issue 9) pp:1536-1543
Publication Date(Web):23 Jul 2008
DOI:10.1039/B806140H
This paper describes a modular approach to constructing microfluidic systems for the generation of gradients of arbitrary profiles. Unlike most current microfluidic-based systems that have integrated architectures, we design several basic component modules such as distributors, combiners, resistors and collectors and connect them into networks that produce gradients of any profile at will. Using the system as a platform we can generate arbitrary gradient profiles that are tunable in real time. The key advantage of this system is that its operation is based on prefabricated components that are relatively simple. Particularly for non-specialists, the modular microfluidic system is easier to implement and more versatile compared to single, integrated gradient generators. The disadvantages associated with this system is that the total amount of liquids used is rather large compared with single chip-based systems. The system would be useful in simulating environments in vivo, e.g., studying how cells respond to temporal and spatial stimuli.
Co-reporter:Yang Zhou;Shixing Wang;Ke Zhang Dr.
Angewandte Chemie International Edition 2008 Volume 47( Issue 39) pp:7454-7456
Publication Date(Web):
DOI:10.1002/anie.200802317
Co-reporter:Yang Zhou;Shixing Wang;Ke Zhang Dr.
Angewandte Chemie 2008 Volume 120( Issue 39) pp:7564-7566
Publication Date(Web):
DOI:10.1002/ange.200802317
Co-reporter:D. Yang;B. Lu;Y. Zhao;X. Jiang
Advanced Materials 2007 Volume 19(Issue 21) pp:3702-3706
Publication Date(Web):5 NOV 2007
DOI:10.1002/adma.200700171
Electrospinning magnetic-nanoparticle-doped polymers under the influence of a magnetic field produces aligned arrays of fibers over large areas (see figure). These nanofibers can be transferred onto the surfaces of other substrates. They can also be stacked into multilayer grids.
Co-reporter:Yong Li;Bo Yuan;Hang Ji ;Dong Han ;Shiqian Chen ;Feng Tian
Angewandte Chemie International Edition 2007 Volume 46(Issue 7) pp:
Publication Date(Web):21 DEC 2006
DOI:10.1002/anie.200603844
Fenced in: Selective electrochemical desorption of self-assembled monolayers of HS(CH2)11(OCH2CH2)6OH was carried out on parts of a gold substrate restricted to microfluidic channels. This chemical transformation activates parts of the surface for adhesion of multiple types of cells with well-controlled geometry.
Co-reporter:Yong Li;Bo Yuan;Hang Ji ;Dong Han ;Shiqian Chen ;Feng Tian
Angewandte Chemie 2007 Volume 119(Issue 7) pp:
Publication Date(Web):21 DEC 2006
DOI:10.1002/ange.200603844
Eingezäunt: Durch selektive elektrochemische Desorption selbstorganisierter Monoschichten aus HS(CH2)11(OCH2CH2)6OH von den zu Mikrofluidkanälen gehörenden Teilen eines Goldsubstrats werden diese Oberflächen für die Adhäsion einer Vielzahl von Zelltypen in genau festgelegter Anordnung aktiviert.
Co-reporter:Lingmin Zhang, Wenfu Zheng, Rongbing Tang, Nuoxin Wang, Wei Zhang, Xingyu Jiang
Biomaterials (October 2016) Volume 104() pp:
Publication Date(Web):October 2016
DOI:10.1016/j.biomaterials.2016.07.015
We report fluorescent carbon nanoparticle (FCN)-based small interfering RNA (siRNA) conjugates (C-siRNA) for gene regulation and cancer therapy. The C-siRNA has a core of chitosan-derived FCN and a shell of siRNA, and can down-regulate the expression of polo-like kinase-1 (Plk1), a master regulator of mitosis, via siRNA targeting Plk1 (siPlk1), for cancer therapy. The required amount of the FCNs is only ∼1/30 of that of the gold nanoparticles in delivering equal amount of siRNA. The C-siPlk1 led to ∼80% knockdown of cellular Plk1 mRNA in A375 cells, and induced apoptosis of the A375 cells (31.9%) and MCF-7 cells (20.33%), much higher than those by commercial nonviral gene delivery vectors, such as Lipofectamine 2000 in both cell lines (apoptosis rate < 10%). After the C-siPlk1 was administrated to A375 tumor-bearing mice intravenously, the tumor volume was less than 1/11 of the control groups. The C-siRNA can thus be powerful tools for gene delivery and gene therapy.
Co-reporter:Lingmin Zhang, Wenfu Zheng, Rongbing Tang, Nuoxin Wang, Wei Zhang, Xingyu Jiang
Biomaterials (October 2016) Volume 104() pp:269-278
Publication Date(Web):October 2016
DOI:10.1016/j.biomaterials.2016.07.015
We report fluorescent carbon nanoparticle (FCN)-based small interfering RNA (siRNA) conjugates (C-siRNA) for gene regulation and cancer therapy. The C-siRNA has a core of chitosan-derived FCN and a shell of siRNA, and can down-regulate the expression of polo-like kinase-1 (Plk1), a master regulator of mitosis, via siRNA targeting Plk1 (siPlk1), for cancer therapy. The required amount of the FCNs is only ∼1/30 of that of the gold nanoparticles in delivering equal amount of siRNA. The C-siPlk1 led to ∼80% knockdown of cellular Plk1 mRNA in A375 cells, and induced apoptosis of the A375 cells (31.9%) and MCF-7 cells (20.33%), much higher than those by commercial nonviral gene delivery vectors, such as Lipofectamine 2000 in both cell lines (apoptosis rate < 10%). After the C-siPlk1 was administrated to A375 tumor-bearing mice intravenously, the tumor volume was less than 1/11 of the control groups. The C-siRNA can thus be powerful tools for gene delivery and gene therapy.
Co-reporter:Yunlei Xianyu, Xingyu Jiang
Current Opinion in Chemical Engineering (May 2014) Volume 4() pp:144-151
Publication Date(Web):1 May 2014
DOI:10.1016/j.coche.2014.01.009
•Overview of the basic components in the analytical process.•Review of strategies for glucose assays with nanoscale materials.•Recent advances in enzymatic and enzyme-free glucose sensing.Glucose sensing remains a major concern worldwide due to the striking and growing number of diabetic patients. Extensive efforts have been made to achieve reliable glucose assays. The burgeoning nanotechnology offers an opportunity to facilitate glucose sensing for the extraordinary properties of nanomaterials. In this article, recent developments will be discussed toward the optical glucose assays based on nanoscale materials and approaches.
Co-reporter:Yapei Zhang, Qing Zhao, Huashan Wang, Xingyu Jiang, Ruitao Cha
Carbohydrate Polymers (15 May 2017) Volume 164() pp:
Publication Date(Web):15 May 2017
DOI:10.1016/j.carbpol.2017.01.096
•The green and gelatin-free capsules were prepared using NCC.•The disintegration time of NCC capsule meets the criteria in USP32-701.•No significant difference in drug release between NCC capsule and gelatin capsule.•NCC capsule supplies a green platform for applications in drug delivery.The ambiguous source and uncontrolled solubility of gelatin make it crucial to develop gelatin capsule substitutes. Nanocrystalline cellulose (NCC) of excellent mechanical performance, good biocompatibility and green origin has drawn many attentions as a new class of biomaterials. In this work, NCC capsule was prepared by dipping method with PEG and glycerol as plasticizer. The effect of PEG and glycerol on the rheological property of NCC gel and the surface morphology of NCC capsule was evaluated. The disintegration time of NCC capsule meet the criteria in United States Pharmacopoeia (USP32-701).There is no significant difference in the release of sulfuric acid between gelatin capsule and NCC capsule. NCC capsule thus has the potential as a green platform for biomedical applications in drug delivery.
Co-reporter:Zhuo Huang and Xingyu Jiang
Journal of Materials Chemistry A 2013 - vol. 1(Issue 46) pp:NaN7662-7662
Publication Date(Web):2013/08/14
DOI:10.1039/C3TC31165A
In recent years, a number of new materials and techniques at the micro/nano-scale for neuroscience have been reported, in particular in studies of neuronal development and electronic addressing. They offer new capabilities to fabricate tools in controlling the surface properties (such as topography and chemistry) as well as executing electrical stimulations and measurements. Here we review the basic principles of these micro/nano-scale materials and highlight the important advances in this field, and finally provide some perspectives for the future.
Co-reporter:Kui Zhu, Jianzhong Shen, Richard Dietrich, Andrea Didier, Xingyu Jiang and Erwin Märtlbauer
Chemical Communications 2014 - vol. 50(Issue 6) pp:NaN678-678
Publication Date(Web):2013/11/05
DOI:10.1039/C3CC48100J
A cellular logic system capable of combinatorial and sequential logic operations based on bacterial protein-triggered cytotoxicity was constructed. Advanced devices such as a keypad lock, half-adder and several basic Boolean properties were demonstrated on the cells.
Co-reporter:Jiashu Sun, Yunlei Xianyu and Xingyu Jiang
Chemical Society Reviews 2014 - vol. 43(Issue 17) pp:NaN6253-6253
Publication Date(Web):2014/06/02
DOI:10.1039/C4CS00125G
One of the goals of point-of-care (POC) is a chip-based, miniaturized, portable, self-containing system that allows the assay of proteins, nucleic acids, and cells in complex samples. The integration of nanomaterials and microfluidics can help achieve this goal. This tutorial review outlines the mechanism of assaying biomarkers by gold nanoparticles (AuNPs), and the implementation of AuNPs for microfluidic POC devices. In line with this, we discuss some recent advances in AuNP-coupled microfluidic sensors with enhanced performance. Portable and automated instruments for device operation and signal readout are also included for practical applications of these AuNP-combined microfluidic chips.
Co-reporter:Mengmeng Li, Yun-Ze Long, Dayong Yang, Jiashu Sun, Hongxing Yin, Zhili Zhao, Wenhao Kong, Xingyu Jiang and Zhiyong Fan
Journal of Materials Chemistry A 2011 - vol. 21(Issue 35) pp:NaN13162-13162
Publication Date(Web):2011/08/08
DOI:10.1039/C1JM12240A
We report on a novel spinning technique, termed double-spinning (DS) by combining electro-spinning (ES) and centrifuge-spinning (CS), to fabricate superfine polymer fibers. With a low operating voltage and a slow rotating speed, DS can generate excellent aligned fibrous arrays and two-layer grids with different angles.
Co-reporter:Wenjing Lu, Jiashu Sun and Xingyu Jiang
Journal of Materials Chemistry A 2014 - vol. 2(Issue 17) pp:NaN2380-2380
Publication Date(Web):2014/01/24
DOI:10.1039/C3TB21478H
Electrospinning technology underwent rapid development in recent years, which can be used for fabricating electrospun fibers with different morphologies and multidimensional structures. These fibers are widely applied in medical diagnosis, tissue engineering, replica molding and other applications. Here we review the recent advances in the electrospinning technology, especially technical progress in fabricating electrospun fibers and assemblies with multidimensional structures, and the biomedical applications of these fibers.
Co-reporter:Ying Li, Hua Jiang, Wenfu Zheng, Niya Gong, Lili Chen, Xingyu Jiang and Guang Yang
Journal of Materials Chemistry A 2015 - vol. 3(Issue 17) pp:NaN3507-3507
Publication Date(Web):2015/03/17
DOI:10.1039/C4TB01819B
Bacterial cellulose (BC), a network of pure cellulose nanofibers with fine crystallinity, high mechanical strength and wet capability, and good biocompatibility, is a good material candidate for wound dressing. Hyaluronan (HA) has obvious curative properties, promoting the healing of wound skin tissue and reducing scar formation. This study explored an “orifice plate” culture method to obtain BC samples of different sizes but consistent thicknesses. Novel BC–HA nanocomposites with a 3-D network structure were obtained through a solution impregnation method. The total surface area and the pore volume of the BC–HA composite films gradually decreased with the increase of HA content. The elongation of BC–HA composite films at the break point gradually increased as the HA content increased while the tensile strength of the BC–HA composite films decreased during the same process. The BC–HA composite films had a better water uptake capability than pure BC, and water vapor transmission rate (WVTR) measurements showed that the BC–HA composite films can satisfy breathing requirements of injured skin. The BC–HA composite films facilitated the growth of primary human fibroblast cells, showing their low toxicity, and the BC–HA composite films with 0.1% HA lead to higher levels of cell viability than the pure BC. In vivo experiments indicated that the BC–HA with 0.1% HA had the shortest wound healing time while BC–HA with 0.05% HA yielded best tissue repair results. The BC–HA composite films are expected to be useful as novel wound dressing materials for clinical skin repair.
Co-reporter:Yi Zhang, Xuwei Wang, Lusheng Song, Chuanlai Xu, Liying Ma, Zhanhua Li, Jianzhong Xi and Xingyu Jiang
Analytical Methods (2009-Present) 2012 - vol. 4(Issue 10) pp:NaN3470-3470
Publication Date(Web):2012/07/19
DOI:10.1039/C2AY25485A
High-throughput assays necessitate high-throughput data analysis. Arrayed microfluidic immunoassay shows the capability of high-throughput protein detection. However, its development was restricted by the low efficiency of downstream data analysis. We present herein programming-based image processing through the local recognition of a sub-array followed by the region-growing algorithm to achieve fast, convenient and precise extraction of information with reduced personal bias.
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