Chen Yao

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Name: 姚琛; Chen Yao

Organization: Southeast University , China

Department:

Title: Associate Professor(PhD)

Chemicals
References

Novel dual VES phospholipid self-assembled liposomes with an extremely high drug loading efficiency

Co-reporter:Yongpeng Hou, Chen Yao, Longbing Ling, Yawei Du, Ruiyu He, Muhammad Ismail, Ying Zhang, Zhenglin Fu, Xinsong Li

Colloids and Surfaces B: Biointerfaces 2017 Volume 156(Volume 156) pp:

Publication Date(Web):1 August 2017

DOI:10.1016/j.colsurfb.2017.05.006

•A novel amphiphilic prodrug (di-VES-GPC) of hydrophobic Vitamin E succinate was successfully synthesized.•Di-VES-GPC assembles into liposomes with high drug loading 82.8 wt%.•The di-VES-GPC liposomes are stable in neutral medium while release VES in weakly acidic environment.•The di-VES-GPC liposomes have comparable in vitro cytotoxicity to VES.Vitamin E succinate (VES), a unique selective anti-cancer drug, has attracted much attention for its ability to induce apoptosis in various cancer cells. Importantly, it has been reported that VES is largely non-toxic to normal cells. However, poor aqueous solubility and bioavailability extensively restricted its clinical utility. In this report, dual VES phospholipid conjugate (di-VES-GPC) prodrug based liposomes were prepared in order to develop an efficient delivery system for VES. Di-VES-GPC was first synthesized by conjugating VES with l-α-glycerophosphorylcholine (GPC) using N,N′-dicyclohexylcarbodiimide (DCC) as a coupling agent. The di-VES-GPC prodrug was able to self-assemble into liposomes by reverse-phase evaporation method. The structure of the liposomes was characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM) and cryo-TEM. The results showed that di-VES-GPC assembled liposomes were spherical with an average diameter approximately 183 nm. Cryo-TEM data confirmed the formation of multilamellar liposomes with the bilayer thickness about 5 nm by the assembly of the conjugate without any excipient. The VES drug loading highly reaches up to 82.8 wt% in the liposomes after a simple calculation. Furthermore, the in vitro release behavior of di-VES-GPC liposomes was evaluated in different media. It was found that the liposomes could release free VES at a weakly acidic microenvironment but exhibited good stability under a simulated biological condition. The cellular uptake and intracellular drug release tests demonstrated that di-VES-GPC liposomes could be internalized effectively and converted into parent drug VES in cancer cells. Furthermore, in vitro antitumor activities of the di-VES-GPC liposomes were evaluated by MTT assay and flow cytometry. It was revealed that the liposomes presented comparable cytotoxicities to free VES. Taken together, the di-VES-GPC liposomes might provide an excellent formulation of VES which have potential in the treatment of cancers.Download high-res image (101KB)Download full-size image

Zein nanofibrous membranes as templates for biomineralization of hydroxyapatite crystallites

Co-reporter:Chen Yao;Yueling Li ;Feifei Wu

Polymer Composites 2013 Volume 34( Issue 7) pp:1163-1171

Publication Date(Web):

DOI:10.1002/pc.22525

Bio-inspired approaches for mimicking the mineralization process of hydroxyapatite (HAp) with highly regulated structures have been intensively developed using biomacromolecular templates. In the present study, zein, a hydrophobic natural protein, was explored to induce the biomineralization of HAp nanocrystallites on the unique nanofibrous structures generated by electrospinning. Zein electrospun fibers with diameters range from several hundred nanometers down to 100 nm were obtained using formic acid as solvent. Randomly oriented apatite crystals were observed on zein nanofibrous membranes after several incubating cycles. The nature of the deposited mineral phase was confirmed as HAp crystallites by energy-dispersive spectroscopy, Fourier transform infrared spectroscopy, and wide-angle X-ray diffraction. A preliminary study of in vitro cytotoxicity and osteoblast adhesion revealed that mineralized zein nanofibrous membranes did not induce cytotoxic effects and had a positive effect on osteoblast growth. Zein/HAp membranes possessed the unique nanofibrous structural features and coating of HAp nanocrystallites may have a wide variety of potential applications for bone repair and regeneration. POLYM. COMPOS., 34:1163–1171, 2013. © 2013 Society of Plastics Engineers

Mineralization of hydroxyapatite crystallites on zein microspheres

Co-reporter:Yueling Li

Polymer Composites 2012 Volume 33( Issue 6) pp:961-966

Publication Date(Web):

DOI:10.1002/pc.22202

Abstract

Bioinspired mineralization process has been extended to the formation of bonelike hydroxyapatite (HAp) coatings on biodegradable polymer substrates. HAp-coated zein microspheres were prepared through phase separation procedure, followed by incubation in modified simulated body fluids (mSBF). The morphologies of mineralized zein microspheres with different diameters and incubation time were investigated by scanning electron microscopy (SEM). The nature of the deposited mineral phase was characterized by energy-dispersive spectroscopy (EDS) and wide-angle X-ray diffraction (XRD). The results showed that as the average diameter of zein microspheres increased to 1.13 μm, randomly oriented minerals were observed on the surface of microspheres. Two characteristic HAp diffraction reflections 002 and 211 centered at 2θ of 25.6° and 32.0° were ascertained on the XRD patterns of the mineralized microspheres. Incubation of zein microspheres with larger diameters in mSBF led to nucleation and growth of minerals on the surface. Even larger number of minerals were deposited and a porous structure of platelike HAp crystals was obtained after incubation for 21 days. The novel zein/HAp microspheres may have a wide variety of potential applications in bone regeneration and tissue engineering. POLYM. COMPOS., 2012. © 2012 Society of Plastics Engineers