Yong Hu

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Name: 胡勇; Yong Hu

Organization: Nanjing University

Department: Institute of Materials Engineering

Title: Professor

TOPICS

Organic Chemistry

Cyclodextrin

Chemicals
References

Hypoxia-Targeting, Tumor Microenvironment Responsive Nanocluster Bomb for Radical-Enhanced Radiotherapy

Co-reporter:Da Huo, Sen Liu, Chao Zhang, Jian He, Zhengyang Zhou, Hao Zhang, and Yong Hu

ACS Nano October 24, 2017 Volume 11(Issue 10) pp:10159-10159

Publication Date(Web):October 9, 2017

DOI:10.1021/acsnano.7b04737

Although ultrasmall metal nanoparticles (NPs) have been used as radiosensitizers to enhance the local damage to tumor tissues while reducing injury to the surrounding organs, their rapid clearance from the circulatory system and the presence of hypoxia within the tumor continue to hamper their further application in radiotherapy (RT). In this study, we report a size tunable nanocluster bomb with a initial size of approximately 33 nm featuring a long half-life during blood circulation and destructed to release small hypoxia microenvironment-targeting NPs (∼5 nm) to achieve deep tumor penetration. Hypoxic profiles of solid tumors were precisely imaged using NP-enhanced computed tomography (CT) with higher spatial resolution. Once irradiated with a 1064 nm laser, CT-guided, local photothermal ablation of the tumor and production of radical species could be achieved simultaneously. The induced radical species alleviated the hypoxia-induced resistance and sensitized the tumor to the killing efficacy of radiation in Akt-mTOR pathway-dependent manner. The therapeutic outcome was assessed in animal models of orthotopical breast cancer and pancreatic cancer, supporting the feasibility of our combinational treatment in hypoxic tumor management.Keywords: hypoxia; nanoparticles; photothermal therapy; radiosensitization; radiotherapy; tungsten;

Fluorescence guided photothermal/photodynamic ablation of tumours using pH-responsive chlorin e6-conjugated gold nanorods

Co-reporter:Chao Zhang, Xiao Cheng, Mengkun Chen, Jie Sheng, Jing Ren, Zhongying Jiang, Jianfeng Cai, Yong Hu

Colloids and Surfaces B: Biointerfaces 2017 Volume 160(Volume 160) pp:

Publication Date(Web):1 December 2017

DOI:10.1016/j.colsurfb.2017.09.045

•We developed a kind of pH-responsive chlorin e6-conjugated gold nanorods (Ce6-PEG-AuNR).•Ce6-PEG-AuNR showed pH activated fluorescent imaging ability and PTT/PDT properties.•They exhibited high anti-tumour properties in vitro and in vivo.•Ce6-PEG-AuNR could serve as fluorescence guided photothermal/photodynamic therapy agents.Photothermal/photodynamic therapies (PTT/PDT) have been widely accepted as non-invasive therapeutic modalities to erase tumours. However, both therapies face the problem of precisely locating tumours and reducing their side effects. Herein, chlorin e6 conjugated gold nanorod, (Ce6-PEG-AuNR), a type of gold nanorod-photosensitizer conjugate, is designed as a kind of nano-therapeutic agent to simultaneously realize combined PTT/PDT. Compared to free Ce6, the fluorescence of Ce6 adhered to the conjugate is effectively quenched by the longitudinal surface plasmon resonance (LSPR) of in the Ce6-PEG-AuNR. However, the specific fluorescence of Ce6 can be recovered in tumour tissue when Ce6 is separated from the conjugate owing to the cleavage of hydrazone bond between Ce6 and PEG caused by intracellular acidic conditions in tumour tissue. Based on this effect, we can precisely locate tumours and further kill cancer cells by combined PTT/PDT. In addition, the combined therapy (PTT/PDT) function is more efficient in cancer treatment than that of PTT or PDT alone. Therefore, Ce6-PEG-AuNR can serve as a promising dual-modal phototherapeutic agent as well as a tumour-sensitive fluorescent probe to diagnose and treat cancer.Download high-res image (211KB)Download full-size image

Anti-RhoJ antibody functionalized Au@I nanoparticles as CT-guided tumor vessel-targeting radiosensitizers in patient-derived tumor xenograft model

Co-reporter:Sen Liu, Hui Li, Luyao Xia, Peipei Xu, Yin Ding, Da Huo, Yong Hu

Biomaterials 2017 Volume 141(Volume 141) pp:

Publication Date(Web):1 October 2017

DOI:10.1016/j.biomaterials.2017.06.036

The clinical success of radiotherapy is greatly hampered due to its intolerable off-target cytotoxicity induced by the high dose of radiation. Meanwhile, low dose of irradiation greatly potentiates the intratumoral angiogenesis, which promotes the local relapse and metastasis of tumor. Therefore, it is essential to reduce the irradiation dosage while inhibiting the tumor angiogenesis during radiotherapy. In this work, tumor vessel specific ultrafine Au@I nanoparticles (AIRA NPs) are fabricated and used as targeted radiosensitizers. Due to the presence of Au and iodine, these AIRA NPs exhibit superb X-ray attenuation for contrast-enhanced computed tomography (CT). Once injected, these AIRA NPs bind specifically to both newly formed tumor vessels in peri- and intratumoral regions and pre-existing tumor vessels. Upon radiation under CT guidance, AIRA NPs remarkably enhanced the killing efficacy against tumors in vivo with respect to radiation alone or anti-angiogenesis chemotherapy. Meanwhile, down-regulation of the level of circulating VEGF cytokine further indicates that our strategy can eradicate tumor without risking the recurrence of hypoxia and angiogenesis. Our demonstration provides a robust method of cancer therapy integrating good biocompatibility, high specificity and relapse-free manner alternative to traditional metal NPs enhanced radiotherapy.Anti-RhoJ antibody functionalized hybrid nanoparticles are used as tumor vessel targeting radiosensitizers. By taking patient-derived tumor model, this platform can precisely target the tumor vasculature under CT guidance, and locally inhibit angiogenesis upon low-dosage of radiation in relapse-free manner.Download high-res image (258KB)Download full-size image

Synthesis and characterization of lignosulfonate-graft-poly (acrylic acid)/hydroxyethyl cellulose semi-interpenetrating hydrogels

Co-reporter:Jiaojiao Zhao, Kun Zheng, Jingya Nan, Chao Tang, Ying Chen, Yong Hu

Reactive and Functional Polymers 2017 Volume 115(Volume 115) pp:

Publication Date(Web):1 June 2017

DOI:10.1016/j.reactfunctpolym.2017.03.019

Maximizing the use of waste is an important part of the strategy for sustainable development. Lignosulfonate, a waste product with sufficient reactive functional groups, can be used as reinforcing materials in polymer composites. In this work, composite hydrogels composed of lignosulfonate-graft-poly (acrylic acid) AA network and hydroxyethyl cellulose (HEC) polymer chains are synthesized through in situ polymerization and cross-linking reaction. The composite hydrogels have semi-interpenetrating network (semi-IPNs) structure, which is driven by the hydrogen bond interactions between proton-donating PAA and proton-accepting HEC. The mechanical properties of these composite hydrogels, including fracture stress, critical compression and elastic modulus and elongation are investigated by tensile measurements. These composite hydrogels exhibit higher toughness and extensibility compared to conventional PAA polymer hydrogels. Moreover, full recovery of their original shape after the removal of compression stress indicates their excellent shape-recovery property. Due to their porous structure, these hydrogels show stimuli responsive swelling properties in aqueous solution depending on the pH or ionic strength, which facilitate the repeating absorption and removal of dyes. Therefore, this work may open a new pathway to synthesize functional materials based on lignosulfonate.

Anti-Fas Antibody Conjugated Nanoparticles Enhancing the Antitumor Effect of Camptothecin by Activating the Fas–FasL Apoptotic Pathway

Co-reporter:Hongliang Yu, Jian He, Qian Lu, Da Huo, Shanmei Yuan, Zhengyang Zhou, Peipei Xu, and Yong Hu

ACS Applied Materials & Interfaces 2016 Volume 8(Issue 44) pp:29950

Publication Date(Web):October 18, 2016

DOI:10.1021/acsami.6b09760

Emerging evidence suggest that the introduction of Fas ligand (FasL) can enhance the Fas-dependent apoptosis and induce durable immune responses against tumor. However, selective triggering of apoptosis in tumor cells while sparing normal cells remains a great challenge for the application of FasL-based therapeutic strategies. Herein, smart nanoparticles (NPs) with a sandwich structure were fabricated. These NPs consist of a matrix metalloproteinase (MMP) cleavable PEG outer layer, an anti-Fas antibody middle layer, and a camptothecin (CPT)-loaded inner core. They could accumulate at a tumor site by the enhanced permeability and retention (EPR) effect. The removable PEG layer protects the cytotoxic anti-Fas antibody from premature contact with normal tissues, thus avoiding the unexpected lethal side effect before they reach the tumor site. Due to the high level of MMP expressed by tumor cells inside the tumor tissue, these NPs would shed their PEG layers, resulting in the exposure of anti-Fas antibody to bind the Fas receptor and triggering the apoptosis of tumor cells. Results of Western blot confirmed that these NPs could mimic the function of activated cytotoxic lymphocyte (CTL) to activate the Fas–FasL apoptosis pathway of tumor cells. With the aid of CPT payload, these anti-Fas antibody conjugated NPs achieved a high tumor inhibition in the B16 allograft tumor animal model. The design of these NPs provides a method for delivering cytotoxic ligand to targeting tissue, which may be valuable in cancer therapy.Keywords: anti-Fas antibody; camptothecin; matrix metalloproteinase; nanoparticles; polycaprolactone

Small Antimicrobial Agents Based on Acylated Reduced Amide Scaffold

Co-reporter:Peng Teng, Da Huo, Alekhya Nimmagadda, Jianfeng Wu, Fengyu She, Ma Su, Xiaoyang Lin, Jiyu Yan, Annie Cao, Chuanwu Xi, Yong Hu, and Jianfeng Cai

Journal of Medicinal Chemistry 2016 Volume 59(Issue 17) pp:7877-7887

Publication Date(Web):August 15, 2016

DOI:10.1021/acs.jmedchem.6b00640

Prevalence of drug-resistant bacteria has emerged to be one of the greatest threats in the 21st century. Herein, we report the development of a series of small molecular antibacterial agents that are based on the acylated reduced amide scaffold. These molecules display good potency against a panel of multidrug-resistant Gram-positive and Gram-negative bacterial strains. Meanwhile, they also effectively inhibit the biofilm formation. Mechanistic studies suggest that these compounds kill bacteria by compromising bacterial membranes, a mechanism analogous to that of host-defense peptides (HDPs). The mechanism is further supported by the fact that the lead compounds do not induce resistance in MRSA bacteria even after 14 passages. Lastly, we also demonstrate that these molecules have therapeutic potential by preventing inflammation caused by MRSA induced pneumonia in a rat model. This class of compounds could lead to an appealing class of antibiotic agents combating drug-resistant bacterial strains.

Paclitaxel-Loaded -Cyclodextrin-Modified Poly(Acrylic Acid) Nanoparticles through Multivalent Inclusion for Anticancer Therapy

Co-reporter:Shanmei Yuan;Jiao Chen;Jie Sheng;Zhongying Jiang

Macromolecular Bioscience 2016 Volume 16( Issue 3) pp:341-349

Publication Date(Web):

DOI:10.1002/mabi.201500302

Preparation of ALA-loaded PLGA nanoparticles and its application in PDT treatment

Co-reporter:Yanping Guan;Yi Zhou;Jie Sheng;Zhong Ying Jiang;Halydan Jumahan

Journal of Chemical Technology and Biotechnology 2016 Volume 91( Issue 4) pp:1128-1135

Publication Date(Web):

DOI:10.1002/jctb.4697

Abstract

BACKGROUND

Coaxial electrospray is an emerging technology to produce multilayer micro- and nano-particles (NPs) with well controlled shape and size. ALA-PDT is a fascinating technology used in the treatment for skin and other cancers. In this work, ALA-loaded poly(lactide-co-glycolide) (PLGA) (PLGA-ALA) NPs with high drug loading efficiency were produced via coaxial electrospray and used for the PDT treatment in HSC-3 cells.

RESULTS

Results showed that 200–1000 nm PLGA-ALA NPs with 60–75% ALA loading efficiency were successfully produced. ALA and PLGA were well integrated and no extra chemical reaction occurred during the electrospray. PLGA-ALA NPs displayed a delayed release of ALA in PBS solution up to 7 days. A massive accumulation of PpIX in HSC-3 cells happened after the incubation of PLGA-ALA NPs. With light irradiation, these PLGA-ALA NPs showed a remarkable cytotoxicity against HSC-3 cells, leading to 40–70% cell death depending on the incubation dose.

CONCLUSIONS

Coaxial electrospraying is a facile method to prepare PLGA-ALA NPs with high drug loading content and efficiency, and PLGA-ALA NPs turned out to be an effective vehicle for delivering ALA to tumor cells. © 2015 Society of Chemical Industry

Enzyme sensitive, surface engineered nanoparticles for enhanced delivery of camptothecin

Co-reporter:Hongliang Yu, Jiao Chen, Sen Liu, Qian Lu, Jian He, Zhengyang Zhou, Yong Hu

Journal of Controlled Release 2015 Volume 216() pp:111-120

Publication Date(Web):28 October 2015

DOI:10.1016/j.jconrel.2015.08.021

To achieve a drug delivery system combining the programmable long circulation and targeting ability, surface engineering nanoparticles (NPs), having a sandwich structure consisting of a long circulating outmost layer, a targeting middle layer and a hydrophobic innermost core were constructed by mixing a matrix metalloproteinase MMP2 and MMP9-sensitive copolymers (mPEG-Pep-PCL) and folate receptor targeted copolymers (FA–PEG–PCL). Their physiochemical traits including morphology, particle size, drug loading content, and in vitro release profiles were studied. In vitro studies validated that the inhibition efficiency of tumor cells was effectively correlated with NP concentrations. Furthermore, The PEG layer would detach from the NPs due to the up-regulated extracellular MMP2 and MMP9 in tumors, resulting in the exposure of folate to enhance the cellular internalization via folate receptor mediated endocytosis, which accelerated the release rate of CPT in vivo. The antitumor efficacy, tumor targeting ability and bio-distribution of the NPs were examined in a B16 melanoma cells xenograft mouse model. These NPs showed improved tumor target ability and enhanced aggregation of camptothecin (CPT) in tumor site and prominent suppression of tumor growth. Thus this mPEG-Pep-PCL@FA–PEG–PCL core–shell structure NP could be a better candidate for the tumor specific delivery of hydrophobic drug.

Fabrication and Characterization of Gd-DTPA-Loaded ChitosanPoly(Acrylic Acid) Nanoparticles for Magnetic Resonance Imaging

Co-reporter:Arsalan Ahmed;Chao Zhang;Jian Guo;Xiqun Jiang

Macromolecular Bioscience 2015 Volume 15( Issue 8) pp:1105-1114

Publication Date(Web):

DOI:10.1002/mabi.201500034

Gd-DTPA-loaded chitosan–poly(acrylic acid) nanoparticles (Gd-DTPA@CS–PAA NPs) were formulated based on the reaction system of water-soluble polymer–monomer pairs of acrylic acid in chitosan solution followed by sorption of Gd-DTPA. Morphological investigations revealed the spherical shape of these NPs with about 220 nm particle size. These NPs showed charge reversal characteristic in acidic solution. In vitro and in vivo magnetic characteristics of these NPs were explored to estimate their utilization in targeted enhanced magnetic resonance imaging. Relaxation studies showed that these NPs possessed pH susceptible relaxation properties, which could introduce in vivo-specific distribution of contrast agent. MRI experiment showed that these nanoparticles had better results in contrast enhancement, and the concentration of contrast agent increased in liver and brain with increment in time. Thus, these NPs could maintain in vivo long circulation and high relaxation rate and were suitable agents for magnetic resonance imaging.

Doxorubicin loaded chitosan–ZnO hybrid nanospheres combining cell imaging and cancer therapy

Co-reporter:Huiyue Zhao, Pin Lv, Da Huo, Chao Zhang, Yin Ding, Peipei Xu and Yong Hu

RSC Advances 2015 vol. 5(Issue 74) pp:60549-60551

Publication Date(Web):08 Jul 2015

DOI:10.1039/C5RA09587E

We report chitosan–ZnO nanoparticles (CZNPs), which have enhanced photoluminescence stability and longer retention time in cells compared with pure ZnO QDs. These CZNPs can illuminate the cells, show the distribution of the nanospheres in the cell, and thus monitor the fate of the loaded drug, which enable these CZNPs to have a promising future in cell imaging and cancer therapy.

Synthesis and application of strawberry-like Fe3O4-Au nanoparticles as CT-MR dual-modality contrast agents in accurate detection of the progressive liver disease

Co-reporter:Hui Y. Zhao, Sen Liu, Jian He, Chao C. Pan, Hui Li, Zheng Y. Zhou, Yin Ding, Da Huo, Yong Hu

Biomaterials 2015 51() pp: 194-207

Publication Date(Web):

DOI:10.1016/j.biomaterials.2015.02.019

Multicomponent Polymeric Nanoparticles Enhancing Intracellular Drug Release in Cancer Cells

Co-reporter:Arsalan Ahmed, Sen Liu, Yutong Pan, Shanmei Yuan, Jian He, and Yong Hu

ACS Applied Materials & Interfaces 2014 Volume 6(Issue 23) pp:21316

Publication Date(Web):October 21, 2014

DOI:10.1021/am5061933

Three kinds of amphiphilic copolymer, that is, poly(ε-caprolactone)-SS-poly(ethylene glycol) (PCL-SS-PEG), poly(ε-caprolactone)-polyethylenimine (PCL-PEI), and poly(ε-caprolactone)-polyethylenimine-folate (PCL-PEI-Fol) were synthesized and self-assembled into surface engineered hybrid nanoparticles (NPs). Morphological studies elucidated the stable, spherical, and uniform sandwich structure of the NPs. PCL-PEI and PCL-SS-PEG segments have introduced pH and reduction responsive characteristics in these NPs, while PCL-PEI-FA copolymers could provide specific targeting capability to cancer cells. The stimuli responsive capabilities of these NPs were carried out. Negative-to-positive charge reversible property, in response to the pH change, was investigated by zeta potential and nuclear magnetic resonance (NMR) measurements. The structure cleavage, due to redox gradient, was studied by dynamic light scattering (DLS) and transmission electron microscopy (TEM). These NPs showed controlled degradation, better drug release, less toxicity, and effective uptake in MCF-7 breast cancer cells. These multifunctional NPs showed promising potential in the treatment of cancer.Keywords: charge reversibility; nanoparticles; poly(ε-caprolactone); surface engineered; targeting

Spatiotemporally Programmable Surface Engineered Nanoparticles for Effective Anticancer Drug Delivery

Co-reporter:Arsalan Ahmed;Hongliang Yu;Dingwang Han;Jingwei Rao;Yin Ding

Macromolecular Bioscience 2014 Volume 14( Issue 11) pp:1652-1662

Publication Date(Web):

DOI:10.1002/mabi.201400228

Abstract

Surface engineered nanoparticles (NPs) are fabricated from polycaprolactone-polyethylenimine-folic acid (PCL-PEI-FA) and polycaprolactone-S-S-polyethylene glycol (PCL–S-S-PEG) copolymers. FESEM reveals the core-shell structure of these NPs of about 230 nm size. It is assumed that the inner cores of these NPs are composed of PCL, while the outer shells are adorned with PEG and folic acid, introducing a stealthy nature and specific targeting capability. Moreover, the disulfide bonds in the PCL–S-S-PEG copolymers provide a reduction-induced degradation characteristic in these NPs. Cell line experiments demonstrate the enhanced endocytosis and cytotoxicity of these NPs. Thus PCL-PEI-FA/PCL-S-S-PEG NPs could be a better candidate for the tumor specific delivery of hydrophobic drugs.

Preparation of water dispersible, fluorescent Ag–PAA–PVP hybrid nanogels and their optical properties

Co-reporter:Yin Ding, Jian Gao, Xiaoyu Yang, Jian He, Zhengyang Zhou, Yong Hu

Advanced Powder Technology 2014 Volume 25(Issue 1) pp:244-249

Publication Date(Web):January 2014

DOI:10.1016/j.apt.2013.04.006

Highlights

•: We develop a novel Ag–PAA–PVA hybrid nanogel.
•: This Ag–PAA–PVA hybrid nanogel is pH sensitive.
•: This nanogel showed surface plasma resonance absorption peak around 420 nm.
•: The nanogels showed photoluminescent properties in fluorescent spectra.

X-ray CT and pneumonia inhibition properties of gold–silver nanoparticles for targeting MRSA induced pneumonia

Co-reporter:Da Huo, Jing Ding, Yi X. Cui, Lu Y. Xia, Hui Li, Jian He, Zheng Y. Zhou, Hong W. Wang, Yong Hu

Biomaterials 2014 35(25) pp: 7032-7041

Publication Date(Web):

DOI:10.1016/j.biomaterials.2014.04.092

X-ray CT guided fault-free photothermal ablation of metastatic lymph nodes with ultrafine HER-2 targeting W18O49 nanoparticles

Co-reporter:Da Huo, Jian He, Hui Li, Ai J. Huang, Hui Y. Zhao, Yin Ding, Zheng Y. Zhou, Yong Hu

Biomaterials 2014 35(33) pp: 9155-9166

Publication Date(Web):

DOI:10.1016/j.biomaterials.2014.07.034

Fabrication of Au@Ag core–shell NPs as enhanced CT contrast agents with broad antibacterial properties

Co-reporter:Da Huo, Jian He, Hui Li, Haiping Yu, Tingting Shi, Yahui Feng, Zhengyang Zhou, Yong Hu

Colloids and Surfaces B: Biointerfaces 2014 Volume 117() pp:29-35

Publication Date(Web):1 May 2014

DOI:10.1016/j.colsurfb.2014.02.008

•We develop a novel kind of m-PEG-Au@Ag core–shell nanoparticles.•m-PEG-Au@Ag nanoparticles showed tunable optical properties.•m-PEG-Au@Ag nanoparticles exhibited low cytotoxicity and significant CT contrast ability both in vitro and in vivo.•These nanoparticles showed remarkable antibacterial properties to both GN and GP bacteria.Au@Ag core–shell nanoparticles (NPs) integrating both antibacterial and X-ray attenuation capabilities were facilely synthesized in aqueous solution. These NPs modified with methoxy-PEG-SH (m-PEG) on the surface rendered them favorable dispersity and stability in water, resulting in enhancement of their blood circulation time. X-ray photoelectron spectroscope (XPS) and high-resolution transmission electron microscope (HRTEM) results confirmed the core–shell structure of m-PEG-Au@Ag NPs. The m-PEG-Au@Ag NPs showed low cytotoxicity and strong X-ray absorption potency in vitro. Further in vivo study showed that as-synthesized NPs offered a pronounced contrast and prolonged their circulation time in the blood stream with negligible toxic effect in vivo. Besides, m-PEG-Au@Ag NPs had significant bacteriostatic effect toward common bacteria like Escherichia coli and Staphylococcus aureus as demonstrated by broth dilution assay. Given their low-cytotoxicity and high CT attenuation efficacy, m-PEG-Au@Ag NPs had a promising potential for use as CT enhancing and antibacterial agents.

Facile synthesis of polymer core@silver shell hybrid nanoparticles with super surface enhanced Raman scattering capability

Co-reporter:Da Huo, Jian He, Shangwen Yang, Zhengyang Zhou, Yong Hu, Matthias Epple

Journal of Colloid and Interface Science 2013 Volume 393() pp:119-125

Publication Date(Web):1 March 2013

DOI:10.1016/j.jcis.2012.11.003

Silver nano-shells (SNSs) were synthesized via a two-step seeds-mediated method. Polymer cores were composed of ultrafine gold nanoparticles (NPs) modified chitosan–poly(acrylic acid) nanoparticles (CS–PAA NPs). Then, deposition of silver upon gold nucleus leads to the seed enlargement and finally forms silver shell on the surface of CS–PAA NPs to get SNSs. Transmission electron microscope (TEM) showed SNSs had a discrete silver shell plus some pores and gaps, which could acted as “hot spots” and provided the great potential of these SNSs to be used as SERS substrates with wavelength ranging from visible to infrared region (700–1000 nm) by tuning shell coverage of silver. SERS experiments with dibenzyl disulphide (DBDS) as the indicator showed that the resulting SNSs allowed the production of highly consistent enhancement of the Raman signals down to nM concentrations of DBDS. Considering the excellent biocompatibility of polymer core and their small size, these SNSs are highly desirable candidates as the enhancers for high performance SERS analysis and as SERS optical labels in biomedical imaging.Graphical abstractHighlights► We developed a kind of silver shell-polymer core hybrid nanoparticles (SNSs). ► These SNSs have a discrete silver shell and show tunable surface plasmon resonance peaks. ► The structure of these SNSs can be controlled by adjusting the experimental condition. ► These SNSs showed a super surface enhanced Raman scattering capability.

Synthesis of β-cyclodextrin modified chitosan–poly(acrylic acid) nanoparticles and use as drug carriers

Co-reporter:Xue Wang, Changjing Chen, Da Huo, Hanqing Qian, Yin Ding, Yong Hu, Xiqun Jiang

Carbohydrate Polymers 2012 Volume 90(Issue 1) pp:361-369

Publication Date(Web):1 September 2012

DOI:10.1016/j.carbpol.2012.05.052

β-Cyclodextrin modified chitosan–poly(acrylic acid) nanoparticles (CS–PAACD NPs) were obtained by polymerizing acrylic acid (AA) and β-cyclodextrin (β-CD) substituted acrylic acid (AACD) in chitosan (CS) solution. These CS–PAACD NPs, characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM) as well as atomic force microscopy (AFM), were quite small in size about 40–50 nm. The size and the microstructure of these CS–PAACD NPs could be accurately controlled by changing the ration of AACD to AA. As the ratio of AACD to AA increased, the size of these NPs decreased. These as-prepared CS–PAACD NPs showed enhanced solubility for paclitaxel (PTX) in aqueous solution and exhibited a typical pH-sensitive release property for the encapsulated drug in vitro. The presence of the β-cyclodextrin inside the CS–PACD NPs greatly enhanced the ability to load hydrophobic drugs, which significantly broadened the application of CS–PAACD NPs in biomedical fields.Highlights► CS–PAACD NPs was obtained by introducing β-CD into CS–PAA nanoparticles. ► The size and the structure of CS–PAACD NPs can be controlled by varying the content of β-CD in them. ► These CS–PAACD NPs had a pH responsible property and excellent encapsulation ability to paclitaxel. ► These paclitaxel loaded CS–PAACD NPs showed high cytotoxicity against C6 glioma cells.

In situ formation of chitosan–gold hybrid hydrogel and its application for drug delivery

Co-reporter:Rui Chen, Qi Chen, Da Huo, Yin Ding, Yong Hu, Xiqun Jiang

Colloids and Surfaces B: Biointerfaces 2012 Volume 97() pp:132-137

Publication Date(Web):1 September 2012

DOI:10.1016/j.colsurfb.2012.03.027

A novel chitosan–gold (CS–Au) hybrid hydrogel was developed from chitosan and chloroauric acid in aqueous solution. Its physiochemical characteristics, including UV absorption, structure, morphology, swelling properties were studied. The CS–Au hybrid hydrogel exhibited an excellent water-absorbing property and could be applied as a drug delivery system for anticancer drug: doxorubicin (DOX) due to its high equilibrium water swelling content. The drug loading and release experiments elicited an efficient drug loading content and sustained drug release pattern. Moreover, DOX released from hydrogel which itself had no cytotoxicity was biological active similar as the free DOX, but lower cytotoxicity due to its controllable release. All proved it an ideal local drug delivery system indicating a promising potential future in medical or pharmaceutical area.Graphical abstractHighlights► We develop a novel chitosan–gold (CS–Au) hybrid hydrogel. ► Au nanoparticles work as physical cross-linking points of the hydrogel. ► The hydrogel exhibits an excellent water-absorbing and re-swelling property. ► The hydrogel can be used as a drug delivery system for doxorubicin (DOX).

Hollow chitosan–silica nanospheres for doxorubicin delivery to cancer cells with enhanced antitumor effect in vivo

Co-reporter:Eryun Yan, Yilong Fu, Xue Wang, Yin Ding, Hanqing Qian, Chi-Hwa Wang, Yong Hu and Xiqun Jiang

Journal of Materials Chemistry A 2011 vol. 21(Issue 9) pp:3147-3155

Publication Date(Web):24 Jan 2011

DOI:10.1039/C0JM03234D

Here we report the synthesis of hybrid hollow chitosan–silica nanospheres (CS–Silica NPs) with chitosan–polyacrylic acid (CS–PAA) nanoparticles as the template and doxorubicin (DOX) delivery based on CS–Silica NPs. The morphology and the microstructure of CS–Silica NPs were characterized by field emission scanning electron microscopy (FESEM) and X-ray photoelectron spectroscopy (XPS). The confocal laser scanning microscopy (CLSM) and flow cytometry experiments showed that the cellular uptake of the DOX-loaded CS–Silica NPs was time dependent. In addition, cellular internalization and intracellular distribution of DOX-loaded CS–Silica NPs indicated that the DOX was mainly distributed in the cell nucleus while the carriers were primarily located in the cytoplasm. In vivo antitumor response indicated that the DOX loaded CS–Silica hybrid hollow nanospheres exhibited superior antitumor effect over the free drugin vivo, which might be ascribable to the enhanced cellular uptake efficiency and the effective delivery of drug to the cell nucleus.

Gold Encapsulated Chitosan-Poly(acrylic acid) Hybrid Hollow Nanospheres

Co-reporter:Yin Ding;Qi Chen;Hanqing Qian;Ying Chen;Wei Wu;Xiqun Jiang

Macromolecular Bioscience 2009 Volume 9( Issue 12) pp:1272-1280

Publication Date(Web):

DOI:10.1002/mabi.200900245

Preparation of porous chitosan-poly(acrylic acid)-calcium phosphate hybrid nanoparticles via mineralization

Co-reporter:ChangJing Chen;Yu Deng;ErYun Yan;XiQun Jiang

Science Bulletin 2009 Volume 54( Issue 18) pp:3127-3136

Publication Date(Web):2009 September

DOI:10.1007/s11434-009-0259-3

In this work, the preparation of chitosan-poly(acrylic acid)-calcium phosphate hybrid nanoparticles (CS-PAA-CaP NP) based on the mineralization of calcium phosphate (CaP) on the surface of chitosan-poly (acrylic acid) nanoparticles (CS-PAA NPs) was reported. CS-PAA-CaP NPs were achieved by directly adding ammonia to the aqueous solution of CS-PAA nanoparticles or by thermal decomposition of urea in the aqueous solution of CS-PAA nanoparticles, resulting in the mineralization of CaP on the surface of CS-PAA NPs. Through these two routes, especially using urea as a pH-regulator, the precipitation of CS-PAA NPs, a common occurrence in basic environment, was avoided. The size, morphology and ingredient of CS-PAA-CaP hybrid nanoparticles were characterized by dynamic light scattering (DLS), transmission electron microscope (TEM), scanning electron microscope (SEM), thermogravimetry analysis (TGA) and X-ray diffractometer (XRD). When urea was used as the pH regulator to facilitate the mineralization during the thermal urea decomposition procedure, regular CS-PAA-CaP hybrid nanoparticles with a porosity-structural CaP shells and 400–600 nm size were obtained. TGA result revealed that the hybrid NPs contained approximately 23% inorganic component, which was consistent with the ratio of starting materials. The XRD spectra of hybrid nanoparticles indicated that dicalcium phosphate (DCP: CaHPO4) crystal was a dominant component of mineralization. The porous structure of the CS-PAA-CaP hybrid NPs might be greatly useful in pharmaceutical and other medical applications.

Hollow chitosan–silica nanospheres for doxorubicin delivery to cancer cells with enhanced antitumor effect in vivo

Co-reporter:Eryun Yan, Yilong Fu, Xue Wang, Yin Ding, Hanqing Qian, Chi-Hwa Wang, Yong Hu and Xiqun Jiang

Journal of Materials Chemistry A 2011 - vol. 21(Issue 9) pp:NaN3155-3155

Publication Date(Web):2011/01/24

DOI:10.1039/C0JM03234D