Co-reporter:Xin Huang, Fengrui Song, Zhiqiang Liu, Shuying Liu and Jun Ai
Analyst 2011 vol. 136(Issue 20) pp:4308-4315
Publication Date(Web):06 Sep 2011
DOI:10.1039/C1AN15527J
Electrospray ionization ion trap multiple-stage tandem mass spectrometry (ESI-MSn) was used to evaluate Fructus Schisandrae of similar species (Schisandra chinensis (Turcz.) Baill. fruits and Schisandra sphenanthera Rehd. et Wils. fruits) and different growth characteristics (color, shape, etc.). The application of chemical pattern recognition in the ESI-MSn data analysis was carried out by principal component analysis (PCA), hierarchical cluster analysis (HCA) and linear discriminant analysis (LDA). Then the antioxidant activity of different Fructus Schisandrae samples were determined by an LC-ESI-MS method and ferric reducing antioxidant power (FRAP) assay. Using the ESI-MSn method coupled with chemical pattern recognition analysis and correlated with the antioxidant activity evaluation, the two similar species were successfully distinguished, thus improving the therapeutic safety and effectiveness. The superior characteristics of Schisandra chinensis (Turcz.) Baill. fruits were obtained and made the selection and breeding of Chinese medicine materials more scientific. This study indicates that ESI-MSn is a valuable tool for the authentication of botanical origin and can also be useful for the quality control of Chinese medicinal herbs.
Co-reporter:Hao Yue, Zifeng Pi, Fengrui Song, Zhiqiang Liu, Zongwei Cai, Shuying Liu
Talanta 2009 Volume 77(Issue 5) pp:1800-1807
Publication Date(Web):15 March 2009
DOI:10.1016/j.talanta.2008.10.022
Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSn and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSn spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.
Co-reporter:Xin Huang, Yan Liu, Fengrui Song, Zhiqiang Liu, Shuying Liu
Talanta 2009 Volume 78(Issue 3) pp:1090-1101
Publication Date(Web):15 May 2009
DOI:10.1016/j.talanta.2009.01.021
Co-reporter:Hao Yue;Zi-feng Pi;Hui-lin Li;Feng-rui Song;Zhi-qiang Liu;Shu-ying Liu
Phytochemical Analysis 2008 Volume 19( Issue 2) pp:141-147
Publication Date(Web):
DOI:10.1002/pca.1027
Abstract
The stability of diester-diterpenoid alkaloids (DDA) from plants of the genus Aconitum L. has been studied in different solvents and pH buffers. The HPLC/ESIMS method for analysing the concentration of DDA was established and DDA's decomposition products were elucidated by HPLC/ESI-MS/MSn. In different solvents, e.g. dichloromethane, ether, methanol and distilled water, the decomposition pathways of DDA are quite different and their difference in stabilities depends on the difference of their structures, in which substituents at the N atom and substituents at C-3 are different. The pyrolytic products of DDA, such as deacetoxy aconitine-type alkaloids, have been observed in the above solvents, whereas 8-methoxy-14–benzoyl aconitine-type alkaloids have been obtained only in methanol. Furthermore, the experimental results demonstrate that the stability of DDA depends on pH values of the buffer. Aconine as hydrolysate has been only found in pH 10.0 buffer, and the other hydrolysates and the pyrolyzates of DDA, such as benzoylaconine and deacetoxy aconitine, have been observed in all pH aqueous solutions. The decomposition pathways of DDA in buffers are related to the substituent on the C-3 position. The decomposition pathway of aconitine is similar to that of mesaconitine, but different from that of hypaconitine. Copyright © 2007 John Wiley & Sons, Ltd.
Co-reporter:Hao YUE, Zi-feng PI, Yu-feng ZHAO, Feng-rui SONG, Zhi-qiang LIU, Shu-ying LIU
Chemical Research in Chinese Universities 2007 Volume 23(Issue 5) pp:625-627
Publication Date(Web):September 2007
DOI:10.1016/S1005-9040(07)60135-4
Co-reporter:Lei Shi;Zhi-Qiang Liu;Feng-Ri Song;Shu-Ying Liu
Chinese Journal of Chemistry 2001 Volume 19(Issue 11) pp:
Publication Date(Web):26 AUG 2010
DOI:10.1002/cjoc.20010191108
The sample solution of KNO3 is ejected into the gas phase and the ionic dusters of K+(KNO3)n and NO3 (KNO3)m are formed and observed by electrospray ionization mass spectrometry (ESIMS). Hie full mass spectra of both the positive ion and the negative ion show that the differences between each peak nearby are all about 101 (m/z), which correspond to the molecular weight of KNO3. The general formula of the ionic clusters can be assigned as K+(KNO3)n and NO3′-(KNO3)m..
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