Zhen Huang

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Name: Huang, Zhen

Organization: Georgia State University , USA

Department: Department of Chemistry

Title: Professor(PhD)

Chemicals
References

Preface

Co-reporter:Zhen Huang;David M. J. Lilley

Frontiers of Chemical Science and Engineering 2016 Volume 10( Issue 2) pp:177

Publication Date(Web):2016 June

DOI:10.1007/s11705-016-1571-5

Nucleic acid crystallization and X-ray crystallography facilitated by single selenium atom

Co-reporter:Wen Zhang;Jack W. Szostak

Frontiers of Chemical Science and Engineering 2016 Volume 10( Issue 2) pp:196-202

Publication Date(Web):2016 June

DOI:10.1007/s11705-016-1565-3

X-ray crystallography is a powerful strategy for 3-D structure determination of macromolecules, such as nucleic acids and protein-nucleic acid complexes. However, the crystallization and phase determination are the major bottle-neck problems in crystallography. Recently we have successfully developed synthesis and strategy of selenium-derivatized nucleic acids (SeNA) for nucleic acid crystallography. SeNA might not only provide the rational strategies to solve the phase determination problem, but also offer a potential strategy to explore crystallization solutions.

Evolution of Functional Six-Nucleotide DNA

Co-reporter:Liqin Zhang; Zunyi Yang; Kwame Sefah; Kevin M. Bradley; Shuichi Hoshika; Myong-Jung Kim; Hyo-Joong Kim; Guizhi Zhu; Elizabeth Jiménez; Sena Cansiz; I-Ting Teng; Carole Champanhac; Christopher McLendon; Chen Liu; Wen Zhang; Dietlind L. Gerloff; Zhen Huang; Weihong Tan;Steven A. Benner

Journal of the American Chemical Society 2015 Volume 137(Issue 21) pp:6734-6737

Publication Date(Web):May 12, 2015

DOI:10.1021/jacs.5b02251

Axiomatically, the density of information stored in DNA, with just four nucleotides (GACT), is higher than in a binary code, but less than it might be if synthetic biologists succeed in adding independently replicating nucleotides to genetic systems. Such addition could also add functional groups not found in natural DNA, but useful for molecular performance. Here, we consider two new nucleotides (Z and P, 6-amino-5-nitro-3-(1′-β-d-2′-deoxyribo-furanosyl)-2(1H)-pyridone and 2-amino-8-(1′-β-d-2′-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one). These are designed to pair via complete Watson–Crick geometry. These were added to a library of oligonucleotides used in a laboratory in vitro evolution (LIVE) experiment; the GACTZP library was challenged to deliver molecules that bind selectively to liver cancer cells, but not to untransformed liver cells. Unlike in classical in vitro selection, low levels of mutation allow this system to evolve to create binding molecules not necessarily present in the original library. Over a dozen binding species were recovered. The best had Z and/or P in their sequences. Several had multiple, nearby, and adjacent Zs and Ps. Only the weaker binders contained no Z or P at all. This suggests that this system explored much of the sequence space available to this genetic system and that GACTZP libraries are richer reservoirs of functionality than standard libraries.

Fabricating high performance lithium-ion batteries using bionanotechnology

Co-reporter:Xudong Zhang, Yukun Hou, Wen He, Guihua Yang, Jingjie Cui, Shikun Liu, Xin Song and Zhen Huang

Nanoscale 2015 vol. 7(Issue 8) pp:3356-3372

Publication Date(Web):12 Jan 2015

DOI:10.1039/C4NR06815G

Designing, fabricating, and integrating nanomaterials are key to transferring nanoscale science into applicable nanotechnology. Many nanomaterials including amorphous and crystal structures are synthesized via biomineralization in biological systems. Amongst various techniques, bionanotechnology is an effective strategy to manufacture a variety of sophisticated inorganic nanomaterials with precise control over their chemical composition, crystal structure, and shape by means of genetic engineering and natural bioassemblies. This provides opportunities to use renewable natural resources to develop high performance lithium-ion batteries (LIBs). For LIBs, reducing the sizes and dimensions of electrode materials can boost Li+ ion and electron transfer in nanostructured electrodes. Recently, bionanotechnology has attracted great interest as a novel tool and approach, and a number of renewable biotemplate-based nanomaterials have been fabricated and used in LIBs. In this article, recent advances and mechanism studies in using bionanotechnology for high performance LIBs studies are thoroughly reviewed, covering two technical routes: (1) Designing and synthesizing composite cathodes, e.g. LiFePO4/C, Li3V2(PO4)3/C and LiMn2O4/C; and (2) designing and synthesizing composite anodes, e.g. NiO/C, Co3O4/C, MnO/C, α-Fe2O3 and nano-Si. This review will hopefully stimulate more extensive and insightful studies on using bionanotechnology for developing high-performance LIBs.

Synthesis of biocarbon coated Li3V2(PO4)3/C cathode material for lithium ion batteries using recycled tea

Co-reporter:Chuanliang Wei, Wen He, Xudong Zhang, Shujiang Liu, Chao Jin, Shikun Liu and Zhen Huang

RSC Advances 2015 vol. 5(Issue 36) pp:28662-28669

Publication Date(Web):11 Mar 2015

DOI:10.1039/C4RA15592K

A biocarbon coated Li3V2(PO4)3/C (LVP-C) cathode material was synthesized by a facile sol–gel method using recycled tea as both the structural template and biocarbon source. X-ray diffraction (XRD) patterns show that LVP has a monoclinic structure with space group P21/n. High-resolution transmission electron microscopy (HRTEM) images show that the LVP nanoparticles are surrounded by amorphous biocarbon, and the thickness of the biocarbon shell is about 10–20 nm. Electrochemical measurements demonstrate that the LVP-C nanocomposite shows a significantly better rate capability and cycling performance than pure LVP. In the potential range of 3.0–4.3 V, the LVP-C nanocomposite delivers a high initial discharge capacity of 132 mA h g−1 at 0.5 C, and maintains an initial discharge capacity of 110 mA h g−1 at 10 C. After 80 cycles at 10 C, it still retains a discharge capacity of 110 mA h g−1. Electrochemical impedance spectroscopy (EIS) measurements have disclosed that the LVP-C sample exhibits enhanced electrode reaction kinetics and improved electrochemical performance. The good electrochemical performance of the LVP-C nanocomposite is mainly related to the presence of the conductive biocarbon, thus leading to an improvement in the electron and lithium ion diffusivity. These results indicate that the biocarbon coated LVP-C material is a promising candidate for large capacity and high power cathode materials in next generation lithium-ion batteries for electric vehicles.

Synthesis and optical behaviors of 6-seleno-deoxyguanosine

Co-reporter:Manindar Kaur

Science China Chemistry 2014 Volume 57( Issue 2) pp:314-321

Publication Date(Web):2014 February

DOI:10.1007/s11426-013-5038-y

We have developed a simple method to synthesize 6-seleno-2′-deoxyguanosine (SedG) by selectively replacing the 6-oxygen atom with selenium. This selenium-atom-specific modification (SAM) alters the optical properties of the naturally occurring 2′-deoxyguanosine (dG). Unlike the native dG, the UVabsorption of SedG is significantly influenced by the pH of the aqueous solution. Moreover, SedG is fluorescent at the physiological pH and exhibits pH-dependent fluorescence in aqueous solutions. Furthermore, SedG has noticeable fluorescence in non-aqueous solutions, indicating its sensitivity to environmental changes. This is the first time a fluorescent nucleoside by single-atom alteration has been observed. Fluorescent nucleosides modified by a single atom have great potential as molecular probes with minimal perturbations to investigate nucleoside interactions with proteins, such as membrane-transporter proteins.

RNA interference with DNA polymerase and synthesis

Co-reporter:Rob Abdur;JianHua Gan

Science China Chemistry 2014 Volume 57( Issue 7) pp:954-960

Publication Date(Web):2014 July

DOI:10.1007/s11426-014-5115-x

RNA can catalyze and participate in many chemical and biochemical reactions. Non-coding RNAs (ncRNA) can regulate cellular transcription and translation reactions. We have demonstrated biochemically that RNA can also interfere with DNA polymerization via transforming DNA polymerase into deoxyribonucleoside triphosphate diphosphatase (dNTP-DPase). RNA, even with six nucleotides, can transform DNA polymerase into dNTP-DPase, and the dNTP-DPase activity causes the hydrolysis of dNTPs into dNMPs and pyrophosphate. Moreover, we have found that DNA polymerases from several families generally have similar RNA-dependent dNTP-DPase activity. We have also observed that in the presence of RNA, when the dNTP concentrations are relatively low, and that the dNTP-DPase activity can deplete dNTPs and interfere with DNA polymerization. Thus, we have discovered for the first time that in the presence of RNA, DNA polymerase can behave as a diphosphatase and inhibit DNA synthesis when dNTP quantity is low. These in vitro observations might imply a plausible role of RNA in vivo, such as suppressing DNA synthesis during a resting phase (G0) of the cell cycle, when RNA quantity is high and dNTP quantity is low.

Synthesis of 6-Se-Guanosine RNAs for Structural Study

Co-reporter:Jozef Salon, Jianhua Gan, Rob Abdur, Hehua Liu, and Zhen Huang

Organic Letters 2013 Volume 15(Issue 15) pp:3934-3937

Publication Date(Web):July 16, 2013

DOI:10.1021/ol401698n

6-Se-guanosine phosphoramidite and RNAs have been synthesized by selenium substitution of the 6-oxygen atom, and it is revealed that the Se-derivatization is relatively stable and that bulge and wobble structures can better accommodate a large Se atom than a duplex. This Se-modification is useful in the structural study of RNAs and their protein complexes.

Synthesis of novel di-Se-containing thymidine and Se-DNAs for structure and function studies

Co-reporter:Wen Zhang;E. Abdalla Hassan

Science China Chemistry 2013 Volume 56( Issue 3) pp:273-278

Publication Date(Web):2013 March

DOI:10.1007/s11426-012-4800-x

The selenium derivatization of nucleic acids and nucleic acid-protein complexes has provided a powerful tool to solve phase problem in X-ray crystallography. Selenium atoms in the nucleotides can serve as fine scattering centers in crystal diffraction. Towards the synthesis of multiple selenium atom-containing nucleotides, which offers strong phasing power to facilitate crystal structure determination, we report here the synthesis of the thymidine analogue containing two Se atoms in one nucleobase. The novel Se-containing nucleoside and oligonucleotide DNAs were synthesized and found with the red-shifted UV spectrum and yellow color. Their unique properties are useful in phase determination, nucleic acid-based detection as well as spectroscopic studies of nucleic acids and nucleic acid-protein complexes.

Convenient synthesis of nucleoside 5′-(α-P-thio)triphosphates and phosphorothioate nucleic acids (DNA and RNA)

Co-reporter:Julianne Caton-Williams;Bilal Fiaz;Rudiona Hoxhaj

Science China Chemistry 2012 Volume 55( Issue 1) pp:80-89

Publication Date(Web):2012 January

DOI:10.1007/s11426-011-4453-1

Modified deoxy- and ribo-nucleoside triphosphates are chemically synthesized in multiple steps due to the protection and deprotection of the nucleoside functionalities. To conveniently synthesize the S-modified triphosphates for enzymatically preparing phosphorothioate DNAs and RNAs (PS-DNA and PS-RNA) as potential therapeutics, herein we report a one-pot strategy to synthesize the deoxy- and ribo-nucleoside 5′-(α-P-thio)triphosphates (dNTPαS and NTPαS) without protecting any nucleoside functionalities. This facile synthesis is achieved by treating the nucleosides with a mild phosphitylating reagent, reacting selectively with the 5′-hydroxyl group of each unprotected nucleoside, followed by sulfurization and hydrolysis to afford the crude dNTPαS and NTPαS analogs (mixtures of Sp and Rp diastereomers). We also demonstrated that after just simple precipitation (without HPLC and ion-exchange purification), the quality of the synthesized dNTPαS and NTPαS analogs is excellent for direct DNA polymerization and RNA transcription, respectively. Since Klenow DNA polymerase and T7 RNA polymerase accept the Sp diastereomers of dNTPαS and NTPαS analogs, respectively, while the Rp diastereomers are neither substrates nor inhibitors, the diastereomerically-pure PS-DNAs and PS-RNAs can be conveniently synthesized enzymatically.

Synthesis of 2-Deoxy-5-(methylselenyl)cytidine and Se-DNAs for Structural and Functional Studies

Co-reporter:Wen Zhang;Jia Sheng;Abdalla E. Hassan

Chemistry – An Asian Journal 2012 Volume 7( Issue 3) pp:476-479

Publication Date(Web):

DOI:10.1002/asia.201100898

Nucleic acid X-ray crystallography via direct selenium derivatization

Co-reporter:Lina Lin, Jia Sheng and Zhen Huang

Chemical Society Reviews 2011 vol. 40(Issue 9) pp:4591-4602

Publication Date(Web):13 Jun 2011

DOI:10.1039/C1CS15020K

X-ray crystallography has proven to be an essential tool for structural studies of bio-macromolecules at the atomic level. There are two major bottle-neck problems in the macromolecular crystal structure determination: phasing and crystallization. Although the selenium derivatization is routinely used for solving novel protein structures through the MAD phasing technique, the phase problem is still a critical issue in nucleic acid crystallography. The background and current progress of using direct selenium-derivatization of nucleic acids (SeNA) to solve the phase problem and to facilitate nucleic acid crystallization for X-ray crystallography are summarized in this tutorial review.

Convenient synthesis of nucleoside 5′-triphosphates for RNA transcription

Co-reporter:Julianne Caton-Williams, Lina Lin, Matthew Smith and Zhen Huang

Chemical Communications 2011 vol. 47(Issue 28) pp:8142-8144

Publication Date(Web):14 Jun 2011

DOI:10.1039/C1CC12201K

By generating a selective phosphitylating reagent in situ, nucleoside 5′-triphosphates can be conveniently synthesized in one pot. This novel strategy without nucleoside protection has been developed to largely simplify synthesis of the nucleoside triphosphates. This demonstrated principle can be applied to the 5′-triphosphate synthesis of both native and modified nucleosides.

Synthesis of the 5′-Se-Thymidine Phosphoramidite and Convenient Labeling of DNA Oligonucleotide

Co-reporter:Wen Zhang and Zhen Huang

Organic Letters 2011 Volume 13(Issue 8) pp:2000-2003

Publication Date(Web):March 24, 2011

DOI:10.1021/ol200397c

The synthesis of the 5′-Se-derivatized thymidine and phosphoramidite is reported, and the Se-phosphoramidite was incorporated into DNA. Because of its high nucleophilicity, this 5′-Se-moiety permits convenient postsynthetic modification of oligonucleotides. The proof of principle was successfully demonstrated by incorporating fluorescein into DNA. It was also found that the 5′-dye-labeled Se-DNA can be recognized by DNA polymerase as an efficient primer. This novel strategy opens a new avenue for nucleic acid probe preparation and detection.

Chemical and structural biology of nucleic acids and protein-nucleic acid complexes for novel drug discovery

Co-reporter:JianHua Gan;Jia Sheng

Science China Chemistry 2011 Volume 54( Issue 1) pp:3-23

Publication Date(Web):2011 January

DOI:10.1007/s11426-010-4174-x

Since nucleic acids (DNA and RNA) play very important roles in cells, they are molecular targets of many clinically used drugs, such as anticancer drugs and antibiotics. Because of clinical demands for treating various deadly cancers and drug-resistant strains of pathogens, there are urgent needs to develop novel therapeutic agents. Targeting nucleic acids hasn’t been the mainstream of drug discovery in the past, and the lack of 3D structural information for designing and developing drug specificity is one of the main reasons. Fortunately, many important structures of nucleic acids and their protein complexes have been determined over the past decade, which provide novel platforms for future drug design and discovery. In this review, we describe some useful nucleic acid structures, particularly their interactions with the ligands and therapeutic candidates or even drugs. We summarize important information for designing novel potent drugs and for targeting nucleic acids and protein-nucleic acid complexes to treat cancers and overcome the drug-resistant problems.

Selenium Derivatization of Nucleic Acids for X-Ray Crystal-Structure and Function Studies

Co-reporter:Jia Sheng

Chemistry & Biodiversity 2010 Volume 7( Issue 4) pp:753-785

Publication Date(Web):

DOI:10.1002/cbdv.200900200

Abstract

It is estimated that over two thirds of all new crystal structures of proteins are determined via the protein selenium derivatization (selenomethionine (Se-Met) strategy). This selenium derivatization strategy via MAD (multi-wavelength anomalous dispersion) phasing has revolutionized protein X-ray crystallography. Through our pioneer research, similarly, Se has also been successfully incorporated into nucleic acids to facilitate the X-ray crystal-structure and function studies of nucleic acids. Currently, Se has been stably introduced into nucleic acids by replacing nucleotide O-atom at the positions 2′, 4′, 5′, and in nucleobases and non-bridging phosphates. The Se derivatization of nucleic acids can be achieved through solid-phase chemical synthesis and enzymatic methods, and the Se-derivatized nucleic acids (SeNA) can be easily purified by HPLC, FPLC, and gel electrophoresis to obtain high purity. It has also been demonstrated that the Se derivatization of nucleic acids facilitates the phase determination via MAD phasing without significant perturbation. A growing number of structures of DNAs, RNAs, and protein–nucleic acid complexes have been determined by the Se derivatization and MAD phasing. Furthermore, it was observed that the Se derivatization can facilitate crystallization, especially when it is introduced to the 2′-position. In addition, this novel derivatization strategy has many advantages over the conventional halogen derivatization, such as more choices of the modification sites via the atom-specific substitution of the nucleotide O-atom, better stability under X-ray radiation, and structure isomorphism. Therefore, our Se-derivatization strategy has great potentials to provide rational solutions for both phase determination and high-quality crystal growth in nucleic-acid crystallography. Moreover, the Se derivatization generates the nucleic acids with many new properties and creates a new paradigm of nucleic acids. This review summarizes the recent developments of the atomic site-specific Se derivatization of nucleic acids for structure determination and function study. Several applications of this Se-derivatization strategy in nucleic acid and protein research are also described in this review.

Synthesis and crystal structure study of 2′-Se-adenosine-derivatized DNA

Co-reporter:Jia Sheng;Jozef Salon;JianHua Gan

Science China Chemistry 2010 Volume 53( Issue 1) pp:78-85

Publication Date(Web):2010 January

DOI:10.1007/s11426-010-0012-4

The selenium derivatization of nucleic acids is a novel and promising strategy for 3D structure determination of nucleic acids. Selenium can serve as an excellent anomalous scattering center to solve the phase problem, which is one of the two major bottlenecks in macromolecule X-ray crystallography. The other major bottleneck is crystallization. It has been demonstrated that the incorporated selenium functionality at the 2′-positions of the nucleosides and nucleotides is stable and does not cause significant structure perturbation. Furthermore, it was observed that the 2′-Se-derivatization could facilitate crystallization of oligonucleotides with fast crystal growth and high diffraction quality. Herein, we describe a convenient synthesis of the 2′-Se-adenosine phosphoramidite, and report the first synthesis and X-ray crystal structure determination of the DNA containing the 2′-Se-A derivatization. The 3D structure of 2′-Se-A-DNA decamer [5′-GTACGCGT(2′-Se-A)C-3′]2 was determined at 1.75 Å resolution, the 2′-Se-functionality points to the minor groove, and the Se-modified and native structures are virtually identical. Moreover, we have observed that the 2′-Se-A modification can greatly facilitate the crystal growth with high diffraction quality. In conjunction with the crystallization facilitation by the 2′-Se-U and 2′-Se-T, this novel observation on the 2′-Se-A functionality suggests that the 2′-Se moiety is sole responsible for the crystallization facilitation and the identity of nucleobases does not influence the crystal growth significantly.

Direct and Rapid Detection of RNAs on a Novel RNA Microchip

Co-reporter:Sarah M. Spencer;Lina Lin ;Cheng-Feng Chiang;Zhengchun Peng;Peter Hesketh Dr.;Jozef Salon Dr.

ChemBioChem 2010 Volume 11( Issue 10) pp:1378-1382

Publication Date(Web):

DOI:10.1002/cbic.201000170

Synthesis and Crystallographic Analysis of 5-Se-Thymidine DNAs

Co-reporter:Abdalla E. A. Hassan, Jia Sheng, Jiansheng Jiang, Wen Zhang and Zhen Huang

Organic Letters 2009 Volume 11(Issue 12) pp:2503-2506

Publication Date(Web):May 26, 2009

DOI:10.1021/ol9004867

We investigated the possibility of the interaction of 5-CH3 of thymidine and its 5′-phosphate backbone (C−H···O−−PO3 interaction) in DNA via the insertion of the atomic probe (a selenium atom) into the exo-5-position of thymidine (5-Se-T). 5-Se-T was synthesized for the first time, via Mn(OAc)3 assisted electrophilic addition of CH3SeSeCH3 to 3′,5′-di-O-benzoyl-2′-deoxyuridine. The 5-Se-T phosphoramidite was subsequently synthesized and incorporated into DNA in over 99% coupling yield. Biophysical and structural investigations of the 5-Se-T DNAs revealed that the Se-modified and nonmodified DNAs are virtually identical. In addition, the crystallographic analysis of a 5-Se-T DNA strongly suggests a hydrogen-bond formation between the 5-CH3 and 5′-phosphate groups (CH3···PO4− interaction).

Synthesis of the First Tellurium-Derivatized Oligonucleotides for Structural and Functional Studies

Co-reporter:Jia Sheng Dr.;Abdalla E.A. Hassan Dr.

Chemistry - A European Journal 2009 Volume 15( Issue 39) pp:10210-10216

Publication Date(Web):

DOI:10.1002/chem.200900774

Abstract

We report here the first synthesis of Te-nucleoside phosphoramidites and Te-modified oligonucleotides. We protected the 2′-tellurium functionality by alkylation and found that the Te functionality is compatible with solid-phase synthesis and that the Te oligonucleotides are stable during deprotection and purification. In addition, the redox properties of the Te functionalities have been explored. We found that the telluride and telluoxide DNAs are interchangeable by redox reactions. At elevated temperature, the Te-DNA can also be site-specifically fragmented oxidatively or reductively when 2′-TePh functionality is present, whereas elimination of the nucleobase is observed in the presence of 2′-TeMe. Moreover, the stability of the DNA duplexes derivatized with the Te functionalities has been investigated. Our Te derivatization of nucleic acids provides a novel approach for investigating DNA damage as well as for structure and function studies of nucleic acids and their protein complexes.

Biochemistry of Selenium-Derivatized Naturally Occurring and Unnatural Nucleic Acids

Co-reporter:Julianne Caton-Williams

Chemistry & Biodiversity 2008 Volume 5( Issue 3) pp:396-407

Publication Date(Web):

DOI:10.1002/cbdv.200890040

Abstract

Selenium (Se) can provide unique biochemical and biological functions, and properties to macromolecules, including protein and RNA. Although Se has not yet been found in DNA, identification of the presence of Se in natural tRNAs has led to discovery of the naturally occurring 2-selenouridine and 5-[(methylamino)methyl]-2-selenouridine (mnm⁵se²U). The Se-atoms at C(2) of the modified uridines are introduced by 2-selenouridine synthase via displacement of the S-atoms in the corresponding 2-thiouridine nucleotides of the tRNAs, and selenophosphate is used as the Se donor. The research indicated that mnm⁵se²U is located at the first or wobble position of the anticodons in several bacterial tRNAs, including tRNA^Lys, tRNA^Glu, and tRNA^Gln. The 2-seleno functionality on this modified nucleotide probably improves the translation accuracy and/or efficiency. These observations in vivo suggest that the presence of Se can provide natural RNAs with useful properties to better function and survival. To further investigate the biochemical and structural properties of Se-derivatized nucleic acids (SeNA), we have pioneered chemical and enzymatic synthesis of Se-derivatized nucleic acids, and introduced Se into both RNA and DNA at a variety of positions by atom-specific replacement of oxygen. This review outlines the recent advancements in chemical and biochemical syntheses, and studies of SeNAs, and their potential applications in structural and functional investigation of nucleic acids and their protein complexes.

Synthesis and DNA-Polymerase Incorporation of Colored 4-Selenothymidine Triphosphate for Polymerase Recognition and DNA Visualization

Co-reporter:Julianne Caton-Williams Dr.

Angewandte Chemie 2008 Volume 120( Issue 9) pp:1747-1749

Publication Date(Web):

DOI:10.1002/ange.200705213

Synthesis and DNA-Polymerase Incorporation of Colored 4-Selenothymidine Triphosphate for Polymerase Recognition and DNA Visualization

Co-reporter:Julianne Caton-Williams Dr.

Angewandte Chemie International Edition 2008 Volume 47( Issue 9) pp:1723-1725

Publication Date(Web):

DOI:10.1002/anie.200705213

Efficient Enzymatic Synthesis of Phosphoroselenoate RNA by Using Adenosine 5′-(α-P-Seleno)triphosphate

Co-reporter:Nicolas Carrasco Dr.;Julianne Caton-Williams;Gary Brt;Siming Wang Dr.

Angewandte Chemie 2006 Volume 118(Issue 1) pp:

Publication Date(Web):22 NOV 2005

DOI:10.1002/ange.200502215

Selenylierte RNA: Adenosin-5′-(α-P-seleno)triphosphat (ATPαSe) wurde synthetisiert und anschließend enzymatisch effizient und diastereoselektiv in RNA eingebaut (siehe Schema). Die Phosphoroselenoat-RNA wird von Phosphodiesterase langsamer verdaut als nichtmodifizierte RNA. Mit dieser enzymatischen Synthese gelingt allgemein die Erzeugung von Selen-derivatisierten RNAs im Multimilligramm-Maßstab.

Efficient Enzymatic Synthesis of Phosphoroselenoate RNA by Using Adenosine 5-

Co-reporter:Nicolas Carrasco, Julianne Caton-Williams, Gary Brandt, Siming Wang,Zhen Huang

Angewandte Chemie International Edition 2006 45(1) pp:94-97

Publication Date(Web):

DOI:10.1002/anie.200502215

Convenient synthesis of nucleoside 5′-triphosphates for RNA transcription

Co-reporter:Julianne Caton-Williams, Lina Lin, Matthew Smith and Zhen Huang

Chemical Communications 2011 - vol. 47(Issue 28) pp:NaN8144-8144

Publication Date(Web):2011/06/14

DOI:10.1039/C1CC12201K

Nucleic acid X-ray crystallography via direct selenium derivatization

Co-reporter:Lina Lin, Jia Sheng and Zhen Huang