Hong-Yang ZHANG

Name: 胡坪; Hu, Ping

Organization: East-China University of Science & Technology , China

Department: School of Chemistry & Molecular Engineering

Title: Professor(PhD)

Chemicals
References

Tartaric acid induced conversion of protopanaxadiol to ginsenosides Rg3 and Rg5 and their in situ recoveries by integrated expanded bed adsorption chromatography

Co-reporter:Dan Huang;Yang Li;Min Zhang;Shengli Ruan;Hongyang Zhang;Yuerong Wang;Ping Hu

Journal of Separation Science 2016 Volume 39( Issue 15) pp:2995-3001

Publication Date(Web):

DOI:10.1002/jssc.201600269

Panax ginseng has been applied in traditional Chinese medicine for over 2000 years. It is still one of the most popular herbs in recent decades. The prescribed ginseng-containing medicines consist of protopanaxadiol and protopanaxatriol ginsenosides, which are the major constituents of the herb. Minor ginsenosides at low levels in the herb, such as Rg₃ and Rg₅, have attracted more rising attention than the major ones. The existing approaches to prepare Rg₃ and Rg₅ usually rely on either steamed red ginseng as the source or chemical/enzymatic conversion of protopanaxadiol to the targets. It is still highly desirable to effectively achieve such minor components. In this paper, a method integrated extraction of protopanaxadiol and conversion of it to Rg₃ and Rg₅ has been proposed. Protopanaxadiol was extracted and simultaneously converted to Rg₃ and Rg₅ by d,l-tartaric acid. The targets were absorbed by resins on expanded bed adsorption chromatography and were then separated from other ginsenosides in different stages. Compared with conventional methods, the developed process has advantages in shortening time consumption and improving the conversion ratio of protopanaxadiol, which is promising in directly achieving Rg₃ and Rg₅ from P. ginseng.

Organs-on-chips and Its Applications

Co-reporter:Wei SUN, Yu-Qing CHEN, Guo-An LUO, Min ZHANG, Hong-Yang ZHANG, Yue-Rong WANG, Ping HU

Chinese Journal of Analytical Chemistry 2016 Volume 44(Issue 4) pp:533-541

Publication Date(Web):April 2016

DOI:10.1016/S1872-2040(16)60920-9

Microfluidic chips have been proven to be attractive platforms for cell culture in vitro. Microfluidic chip-based organs-on-chips technology has received more attention because it can mimic the complex structures and functions of human organs. In this review, the recent advances of organs-on-chips technology in different organs are introduced, including the build of human physiological models, drug discovery, and toxicology research. The development trend of this technology is also proposed.Organs-on-chips could produce fluid shear stress, mechanical stress, biochemical concentration gradient and other physical and chemical stimuli and precisely mimic the main function of many human organs. It has the potential to establish human physiological models and promote the development of drug discovery, and toxicology research.

Hypercrosslinked strong cation-exchange polymers for selective extraction of serum purine metabolites associated with gout

Co-reporter:Yating Xu, Ju Liu, Hongyang Zhang, Min Jiang, Lingling Cao, Min Zhang, Wei Sun, Shengli Ruan, Ping Hu

Talanta 2016 Volume 151() pp:172-178

Publication Date(Web):1 May 2016

DOI:10.1016/j.talanta.2016.01.014

•A facile and efficient synthesis of HXLPP-SCX material was proposed.•The resins possess ~5 μm particle size with specific surface area of 801 m2/g.•The resins have high IEC, adsorption capacity and selectivity.•The resins as SPE sorbents enabled selective extraction of serum basic purines.•The SPE method was successfully applied for the reliable diagnosis of gout.In this study, hypercrosslinked strong cation-exchange polymer resins (HXLPP-SCX) were synthesized and employed as selective sorbents for the solid-phase extraction (SPE) of basic purine metabolites associated with gout. The HXLPP-SCX material was prepared based on hypercrosslinking reactions and sulfonated with concentrated H2SO4. This synthetic procedure is facile and efficient without using highly toxic reagent. The resulting resins were characterized in the form of monodisperse microspheres (mean diameters of 3‒5 μm) with narrow pore size (2.1 nm) and relatively high specific surface areas (801 m2/g). The polymers also possess high ion-exchange capacity (IEC, 2.22 mmol/g) and good adsorption and selectivity performances for basic compounds. The resins used as SPE sorbents permit the selective enrichment of three pivotal purine metabolites (hypoxanthine, xanthine and inosine) in human serum followed by HPLC analysis. Method validation including linearity range, sensitivity, accuracy and reproducibility were evaluated. This method was exemplarily applied in the analysis of serum purines in gout patients and healthy controls. The present results demonstrate a promising potential of this HXLPP-SCX material for the clinical sample pretreatment.Figure optionsDownload full-size imageDownload as PowerPoint slide

Two-stage fractionation of polar alkaloids from Rhizoma coptidis by countercurrent chromatography considering the strategy of reactive extraction

Co-reporter:Yang Li, Fanfan Cai, Min Zhang, Hongyang Zhang, Yuerong Wang, Ping Hu

Journal of Chromatography A 2015 Volume 1378() pp:58-64

Publication Date(Web):23 January 2015

DOI:10.1016/j.chroma.2014.12.028

•A new way of selective purification of polar components from complex natural sources was offered.•The possible mechanism for reactive extraction on countercurrent chromatography was proposed.•High hydrophobicities of the formed salts allowed the targets to transfer from the aqueous phase to the organic.•Five polar alkaloids from Rhizoma coptidis were separated by countercurrent chromatography in two stages.Separation of polar alkaloids by countercurrent chromatography (CCC) is challengeable due to their close partition behaviors in solvent system. In this paper, a two-stage method for isolation of epiberberine, jatrorrhizine, palmatine, coptisine, and berberine from Rhizoma coptidis was presented. The first stage separation performed on CCC was based on the principle of reactive extraction. Trifluoroacetic acid was acted as a modulator to selectively react with alkaloids, which changed their partition coefficients in solvent system. Purified epiberberine and other partially separated targets were eluted by ammonium adjusted mobile phase. In the second stage, four alkaloids were purified in pH-zone-refining CCC mode. All the targets collected were over 97% pure determined by HPLC. The method developed demonstrates performing of reactive extraction on standard CCC as an option for separation of polar alkaloids from medicinal plants.

Combination of integrated expanded bed adsorption chromatography and countercurrent chromatography for the direct extraction and purification of pseudohypericin and hypericin from St. John's wort (Hypericum perforatumL.)

Co-reporter:Fanfan Cai;Yang Li;Min Zhang;Hongyang Zhang;Yuerong Wang;Ping Hu

Journal of Separation Science 2015 Volume 38( Issue 15) pp:2588-2596

Publication Date(Web):

DOI:10.1002/jssc.201500260

St. John's wort has attracted particular attention because of its beneficial effects as an antidepressant, antiviral, and anticancer agent. A method for the combination of integrated expanded bed adsorption chromatography and countercurrent chromatography for the simultaneous extraction and purification of pseudohypericin and hypericin from the herb is presented in this paper. Firstly, the constituents were extracted and directly adsorbed by expanded bed adsorption chromatography under optimal conditions. The stepwise elution was then performed by expanded bed adsorption chromatography that enriched the targets with higher purities and recoveries compared to other methods. Secondly, the eluent fractions from expanded bed adsorption chromatography were further separated by two-step high-speed countercurrent chromatography. A two-step high-speed countercurrent chromatography method with a biphasic solvent system composed of n-hexane/ethyl acetate/methanol/water with a volume ratio of 1:2:1:2 was performed by stepwise changing the flow rate of the mobile phase. Consequently, 5.6 mg of pseudohypericin and 2.2 mg of hypericin with purities of 95.5 and 95.0%, respectively, were successfully obtained from 40 mg of crude sample.

Development of a Method to Extract and Purify Target Compounds from Medicinal Plants in a Single Step: Online Hyphenation of Expanded Bed Adsorption Chromatography and Countercurrent Chromatography

Co-reporter:Yang Li, Nan Wang, Min Zhang, Yoichiro Ito, Hongyang Zhang, Yuerong Wang, Xin Guo, and Ping Hu

Analytical Chemistry 2014 Volume 86(Issue 7) pp:3373

Publication Date(Web):March 3, 2014

DOI:10.1021/ac4035955

Pure compounds extracted and purified from natural sources are crucial to lead discovery and drug screening. This study presents a novel two-dimensional hyphenation of expanded bed adsorption chromatography (EBAC) and high-speed countercurrent chromatography (HSCCC) for extraction and purification of target compounds from medicinal plants in a single step. The EBAC and HSCCC were hyphenated via a six-port injection valve as an interface. Fractionation of ingredients of Salvia miltiorrhiza and Rhizoma coptidis was performed on the hyphenated system to verify its efficacy. Two compounds were harvested from Salvia miltiorrhiza, one was 52.9 mg of salvianolic acid B with an over 95% purity and the other was 2.1 mg of rosmarinic acid with a 74% purity. Another two components were purified from Rhizoma coptidis, one was 4.6 mg of coptisine with a 98% purity and one was 4.1 mg of berberine with a 82% purity. The processing time was nearly 50% that of the multistep method. The results indicate that the present method is a rapid and green way to harvest targets from medicinal plants in a single step.

Coupling of ultrasound-assisted extraction and expanded bed adsorption for simplified medicinal plant processing and its theoretical model: Extraction and enrichment of ginsenosides from Radix Ginseng as a case study

Co-reporter:Jianing Mi;Min Zhang;Hongyang Zhang;Yuerong Wang;Shikun Wu;Ping Hu

Journal of Separation Science 2013 Volume 36( Issue 3) pp:593-601

Publication Date(Web):

DOI:10.1002/jssc.201200745

A high-efficient and environmental-friendly method for the preparation of ginsenosides from Radix Ginseng using the method of coupling of ultrasound-assisted extraction with expanded bed adsorption is described. Based on the optimal extraction conditions screened by surface response methodology, ginsenosides were extracted and adsorbed, then eluted by the two-step elution protocol. The comparison results between the coupling of ultrasound-assisted extraction with expanded bed adsorption method and conventional method showed that the former was better than the latter in both process efficiency and greenness. The process efficiency and energy efficiency of the coupling of ultrasound-assisted extraction with expanded bed adsorption method rapidly increased by 1.4-fold and 18.5-fold of the conventional method, while the environmental cost and CO₂ emission of the conventional method were 12.9-fold and 17.0-fold of the new method. Furthermore, the theoretical model for the extraction of targets was derived. The results revealed that the theoretical model suitably described the process of preparing ginsenosides by the coupling of ultrasound-assisted extraction with expanded bed adsorption system.

Identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Co-reporter:Hongyang Zhang, Huihua Yang, Min Zhang, Yuerong Wang, Jingrong Wang, Leefong Yau, Zhihong Jiang, Ping Hu

Journal of Food Composition and Analysis 2012 Volume 25(Issue 2) pp:142-148

Publication Date(Web):March 2012

DOI:10.1016/j.jfca.2011.09.004

In this study, a method for the identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) with personal database matching has been developed. Two flavonol glycosides and 20 triterpene glycosides were identified, and in addition the profiles of Luo-Han-Guo samples collected at different growing periods (15-, 40-, and 80-days) were compared. Principle component analysis (PCA) showed a clear separation among the various samples from different growing periods. The content of mogroside V significantly increased, while the level of mogroside IIE class dramatically decreased with an increase of growing time; however, the content of mogroside IV class was found to be high after a 40-day period of accumulation, but it subsequently decreased in the fully ripe period. Finally, 7 mogrosides could be screened as potential markers for discrimination of Luo-Han-Guo samples at different stages. The proposed analytical method combined with multivariate statistical analysis was shown to be a useful tool for investigating the chemical components of Luo-Han-Guo as well as for its quality evaluation.Highlights► An UHPLC/QTOF-MS method with personal database matching was developed for botanic compounds identification. ► Two flavonol- and 20 triterpene glycosides in Luo-Han-Guo extracts were identified. ► Luo-Han-Guo samples of different stages were also comparatively profiled. ► Different period samples were clearly separated in PCA plot. ► 7 mogrosides markers were screened for discrimination of Luo-Han-Guo samples at different stages.

Determination of Homovanilic Acid in Human Urine by Weak Anion-Exchange Hypercrosslinked Polymer Resin-Solid Phase Extraction-High Performance Liquid Chromatography-Ultraviolet Detection Method

Co-reporter:Ping HU, Hong-Yang ZHANG, Dong-Yan MAO, Fang-Hong NING, Rui-Juan WANG, Yue-Rong WANG, Min ZHANG

Chinese Journal of Analytical Chemistry 2012 Volume 40(Issue 8) pp:1175-1180

Publication Date(Web):August 2012

DOI:10.1016/S1872-2040(11)60563-X

A solid phase extraction-high performance liquid chromatography-ultraviolet detection (SPE-HPLC-UV) method was developed for the determination of homovanilic acid (HVA) in human urine. First, a weak anion-exchange hypercrosslinked polymer resin (HXLPP-WAX), whose adsorption performance was investigated, was synthesized as SPE packing material. And then the urinary HVA was selectively absorbed on this sorbent. After elution, the HVA was analyzed on a C18 column with the mobile phase of methanol and aqueous acetic acid (15:85, V/V) at detection wavelength of 280 nm. The method validation results demonstrate that this method has a good linearity in the HVA concentration ranging from 2.26 to 289 mg L−1 and a limit of detection of 0.45 mg L−1. The average recoveries were above 90% and the RSD values were lower than 4.2%. The method was successfully applied to determine the HVA contents ranging from 1.55 to 6.79 mg L−1 in eight healthy human urine samples. The results showed that the proposed method was highly selective, sensitive, accurate, and reliable.

Voltammetric determination of uric acid by using gold nanotubule electrode

Co-reporter:Yue Rong Wang, Ping Hu, Qiong Lin Liang, Guo An Luo, Yi Ming Wang

Chinese Chemical Letters 2007 Volume 18(Issue 9) pp:1111-1114

Publication Date(Web):September 2007

DOI:10.1016/j.cclet.2007.07.034

Gold nanotubule membranes were prepared by using electroless deposition of gold within the pores and surfaces of polycarbonate track-etched membranes. And the gold nanotubule membrane was used as an electrode for determination of uric acid in urine samples for the first time. In Britton–Robinson buffer of pH 4.56, uric acid exhibited well-defined differential pulse voltammograms. And the interference between coexistent ascorbic acid and uric acid was overcome owing to the attractive ability of the gold nanotubule electrode to yield a large anodic peak difference ca. 0.404 V (vs. SCE). The proposed method was then applied to the determination of uric acid in urine without any pretreatment.