Co-reporter:Jing Zeng, Xiuli Zhang, Zhimou Guo, Jiatao Feng, Jianguo Zeng, Xingya Xue, Xinmiao Liang
Journal of Chromatography A 2012 1220() pp: 50-56
Publication Date(Web):
DOI:10.1016/j.chroma.2011.11.043
Co-reporter:Jing Zeng, Xiuli Zhang, Zhimou Guo, Jiatao Feng, Xingya Xue, Xinmiao Liang
Journal of Chromatography A 2011 Volume 1218(Issue 13) pp:1749-1755
Publication Date(Web):1 April 2011
DOI:10.1016/j.chroma.2011.01.079
Recent advances in the theory and application of orthogonal LC separation have allowed for the establishment of a more effective method for the chemical identification of target compounds in complex samples, especially structurally similar compounds. In this study, a new chemical identification method based on orthogonal parallel separation and accurate molecular weight confirmation was developed. An orthogonal separation system consisting of an XTerra MS C18 column, a home-made Click OEG column, and a Click CD column was established for separation and identification. In addition, 82 flavonoids were selected as references, to be used for the construction of a library. Retention times of each reference flavonoid on each column and accurate molecular weights were recorded and imported into a searchable library as “tags” for the unknown screening. For the method validation, two complex mixtures, fractions of Dalbergia odorifera T. Chen and Scutellaria baicalensis Georgi, specifically, were separated and identified. In total, nine compounds were unequivocally identified by retention time and confirmation of accurate molecular weight, demonstrating that this method is suitable and efficient for the chemical identification of complex samples.