Spore-derived mycobionts of the crustose lichen Sarcographa tricosa were cultivated on a malt-yeast extract medium supplemented with 10% sucrose. Chemical investigation of the cultivated colonies led to isolation of three eremophilane-type sesquiterpenes, 3-epi-petasol (1), dihydropetasol (2) and sarcographol (3), together with six known eremophilanes and ergosterol peroxide. These structures were elucidated by spectroscopic and chemical methods. This is the first report of eremophilane-type sesquiterpenes from the cultured mycobionts of lichen.Graphical abstractSpore-derived mycobionts of the crustose lichen Sarcographa tricosa were cultivated on a malt-yeast extract medium supplemented with 10% sucrose. Chemical investigation of the cultivated colonies led to isolation of three eremophilane-type sesquiterpenes, 3-epi-petasol (1), dihydropetasol (2) and sarcographol (3) as well as six known eremophilanes and ergosterol peroxide. Their structures were determined by spectroscopic and chemical methods.
Highlights► Spore-derived mycobionts of the Vietnamese lichen Sarcographa tricosa were cultivated. ► Eremophilane-type sesquiterpenes were isolated from the cultures and their structures were determined. ► This is the first instance of isolation of eremophilane-type sesquiterpenes from lichen mycobionts in culture.
