A novel interface was prepared on glass slides that stabilizes several cast polymers against delamination under conditions necessary for the study of cell surface interactions. This interface was synthesized by deposition of zirconium tetra(tert-butoxide) from the vapor phase onto the glass followed by mild thermolysis, which gives a surface-bound zirconium oxide coating. This oxide coating improved attachment of polymer coatings cast from formic acid or methylene chloride. Nylon, polyurethane, and polyhydroxybutyrate/polyhydroxyvalerate coatings were stable against delamination from the oxide-coated glass following sonication in ethanol for more than 30 min or immersion in water at pH 8 for at least 48 h.Keywords: delamination inhibition; glass surface modification; polymer casting; polymer−glass interface