Three unprecedented purine-containing compounds, named [6]-, [8]-, and [10]-zingerines as they are 5-(6-amino-9H-purin-9-yl) analogs of [6]-, [8]-, and [10]-gingerols, respectively, were isolated from a methanolic extract of ginger rhizomes using a phase trafficking-based method that utilizes solid phase reagents allowing for fast and selective simultaneous separation of basic, acidic, and neutral components of natural products extracts.Graphical abstractIsolation and structural determination of the zingerines, unprecedented purine-containing compounds from ginger rhizomes (Zingiber officinale Roscoe), using a phase-trafficking approach..Highlights► We describe the zingerines, new purine-containing compounds obtained from ginger. ► Zingerines contain a purine ring attached to gingerol-type carbon skeleton. ► Isolation of zingerines was facilitated by using phase-trafficking approach.