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ShuXiang Wang

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Name: 王书香; ShuXiang Wang
Organization: Hebei University
Department: Chemical Biology Key Laboratory of Hebei Province, College of Chemistry and Environmental Science
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TOPICS

A novel dual-emission fluorescent probe for the simultaneous detection of H2S and GSH
Co-reporter:H. Li, W. Peng, W. Feng, Y. Wang, G. Chen, S. Wang, S. Li, H. Li, K. Wang and J. Zhang  
Chemical Communications 2016 vol. 52(Issue 25) pp:4628-4631
Publication Date(Web):26 Feb 2016
DOI:10.1039/C6CC00973E
A novel chlorinated coumarin–malononitrile fluorescent probe with three potential reaction sites, which exhibited highly selective, rapid response, low detection limit, and was capable of the simultaneous detection of H2S (λex/em = 515/564 nm) and GSH (λex/em = 430/517 nm), was first proposed. The probe was successfully applied to dual-channel imaging H2S and GSH in MCF-7 cells.
A novel anticancer theranostic pro-prodrug based on hypoxia and photo sequential control
Co-reporter:Weipei Feng, Chunyue Gao, Wei Liu, Huihui Ren, Chao Wang, Kun Ge, Shenghui Li, Guoqiang Zhou, Hongyan Li, Shuxiang Wang, Guang Jia, Zhenhua Li and Jinchao Zhang  
Chemical Communications 2016 vol. 52(Issue 60) pp:9434-9437
Publication Date(Web):23 Jun 2016
DOI:10.1039/C6CC02932A
A novel anticancer pro-prodrug (GMC-CAE-NO2) with diagnosis and therapy functions based on hypoxia and photo sequential control was designed. It provides a platform for constructing theranostic pro-prodrugs to release active drugs controlled by hypoxic status and UV illumination.
Defect-Related Luminescent Hydroxyapatite-Enhanced Osteogenic Differentiation of Bone Mesenchymal Stem Cells Via an ATP-Induced cAMP/PKA Pathway
Co-reporter:Chao Wang, Dandan Liu, Cuimiao Zhang, Jiadong Sun, Weipei Feng, Xing-Jie Liang, Shuxiang Wang, and Jinchao Zhang
ACS Applied Materials & Interfaces 2016 Volume 8(Issue 18) pp:11262
Publication Date(Web):April 18, 2016
DOI:10.1021/acsami.6b01103
Novel defect-related hydroxyapatite (DHAP), which combines the advantages of HAP and defect-related luminescence, has the potential application in tissue engineering and biomedical area, because of its excellent capability of monitoring the osteogenic differentiation and material biodegradation. Although the extracellular mechanism of DHAP minerals and PO43– functioning in osteogenic differentiation has been widely studied, the intracellular molecular mechanism through which PO43– mediates osteogenesis of bone mesenchymal stem cells (BMSCs) is not clear. We examined a previously unknown molecular mechanism through which PO43– promoted osteogenesis of BMSCs with an emphasis on adenosine–triphosphate (ATP)-induced cAMP/PKA pathway. Our studies showed that DHAP could be uptaken into lysosome, in which PO43– was released from DHAP, because of the acid environment of lysosome. The released PO43– interacted with ADP to form ATP, and then degraded into adenosine, an ATP metabolite, which interacted with A2b adenosine receptor to activate the cAMP/PKA pathway, resulting in the high expression of osteogenesis-related genes, such as Runx2, BMP-2, and OCN. These findings first revealed the function of ATP-metabolism in bone physiological homeostasis, which may be developed to cure bone metabolic diseases.Keywords: ATP metabolism; cAMP/PKA pathway; defect-related hydroxyapatite; mesenchymal stem cell; osteogenic differentiation
Size-dependent cytotoxicity of europium doped NaYF4 nanoparticles in endothelial cells
Co-reporter:Shizhu Chen, Cuimiao Zhang, Guang Jia, Jianlei Duan, Shuxiang Wang, Jinchao Zhang
Materials Science and Engineering: C 2014 Volume 43() pp:330-342
Publication Date(Web):1 October 2014
DOI:10.1016/j.msec.2014.07.029
•NaYF4:Eu3 + nanoparticles with three diameters have been synthesized.•NaYF4:Eu3 + nanoparticles could be uptaken by endothelial cells (ECs).•NaYF4:Eu3 + nanoparticles show a significant cytotoxicity on ECs.•The size of NaYF4:Eu3 + nanoparticles may be important to their toxicology effect.Lanthanide-doped sodium yttrium fluoride (NaYF4) nanoparticles exhibit novel optical properties which make them be widely used in various fields. The extensive applications increase the chance of human exposure to these nanoparticles and thus raise deep concerns regarding their riskiness. In the present study, we have synthesized europium doped NaYF4 (NaYF4:Eu3 +) nanoparticles with three diameters and used endothelial cells (ECs) as a cell model to explore the potential toxic effect. The cell viability, cytomembrane integrity, cellular uptake, intracellular localization, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), apoptosis detection, caspase-3 activity and expression of inflammatory gene were studied. The results indicated that these nanoparticles could be uptaken into ECs and decrease the cell viability, induce the intracellular lactate dehydrogenase (LDH) release, increase the ROS level, and decrease the cell MMP in a size-dependent manner. Besides that, the cells were suffered to apoptosis with the caspase-3 activation, and the inflammation specific gene expressions (ICAM1 and VCAM1) were also increased. Our results suggest that the damage pathway may be related to the ROS generation and mitochondrial damage. The results provide novel evidence to elucidate their toxicity mechanisms and may be helpful for more rational applications of these compounds in the future.
Synthesis, cytotoxicity and DNA-binding properties of Pd(II), Cu(II) and Zn(II) complexes with 4′-(4-(2-(piperidin-1-yl)ethoxy)phenyl)-2,2′:6′,2″-terpyridine
Co-reporter:Wenhao Chu, Yuechai Wang, Siyuan Liu, Xueyun Yang, Shuxiang Wang, Shenghui Li, Guoqiang Zhou, Xinying Qin, Chuanqi Zhou, Jinchao Zhang
Bioorganic & Medicinal Chemistry Letters 2013 Volume 23(Issue 18) pp:5187-5191
Publication Date(Web):15 September 2013
DOI:10.1016/j.bmcl.2013.07.003
Pd(II), Cu(II) and Zn(II) complexes (1–3) based on 4′-(4-(2-(piperidin-1-yl)ethoxy)phenyl)-2,2′:6′,2″-terpyridine were synthesized and characterized by UV, IR, NMR, EPR, HRMS, elemental analyses, and molar conductivity measurements. The cytotoxicity of these complexes against HL-60, BGC-823, KB, Bel-7402, A549, Hela, K562 and MCF-7 cell lines in vitro was measured by MTT method. The DNA binding property of the complexes was evaluated by UV, fluorescence, CD spectroscopies and thermal denaturation. The cytotoxicity of complexes 1 and 3 against all the tested cell lines is better than that of cisplatin. Complexes 1 and 2 exhibit 7- and 4-folds higher cytotoxicity than cisplatin against Bel-7402 cell line. Complex 3 displays the highest cytotoxicity against all the cell lines tested, and shows 7-, 14-, 8-, 11- and 8-folds higher cytotoxicity than cisplatin against Bel-7402, A549, Hela, K562 and MCF-7 cell lines. The complexes bind to DNA via intercalation mode and complex 3 stabilizes the G-quadruplex. The results reveal that all the complexes display high cytotoxicity against all the tested cancer cell lines, and complex 3 is selective for G-quadruplex over duplex DNA.
Synthesis, characterization and cytotoxicity of Pt(II), Pd(II), Cu(II) and Zn(II) complexes with 4-substituted terpyridine
Co-reporter:Shuxiang Wang;Wenhao Chu;Yuechai Wang;Siyuan Liu;Jinchao Zhang;Shenghui Li;Haiying Wei;Guoqiang Zhou;Xinying Qin
Applied Organometallic Chemistry 2013 Volume 27( Issue 7) pp:373-379
Publication Date(Web):
DOI:10.1002/aoc.2988

Eight novel Pt(II), Pd(II), Cu(II) and Zn(II) complexes with 4’-substituted terpyridine were synthesized and characterized by elemental analysis, UV, IR, NMR, electron paramagnetic resonance, high-resolution mass spectrometry and molar conductivity measurements. The cytotoxicity of these complexes against HL-60, BGC-823, KB and Bel-7402 cell lines was evaluated by MTT assay. All the complexes displayed cytotoxicity with low IC50 values (<20 μm) and showed selectivity. Complexes 3, 5, 7 and 8 exerted 9-, 5-, 12- and 7-fold higher cytotoxicity than cisplatin against Bel-7402 cell line. The cytotoxicity of complexes 3, 5, 6, 7 and 8 was higher than that of cisplatin against BGC-823 cell line. Complexes 3, 7 and 8 showed similar cytotoxicity to cisplatin against KB cell line. Complex 7 exhibited higher cytotoxicity than cisplatin against HL-60 cell line. Among these complexes, complex 7 demonstrated the highest in vitro cytotoxicity, with IC50 values of 1.62, 3.59, 2.28 and 0.63 μm against HL-60, BGC-823, Bel-7402 and KB cells lines, respectively. The results suggest that the cytotoxicity of these complexes is related to the nature of the terminal group of the ligand, the metal center and the leaving groups. Copyright © 2013 John Wiley & Sons, Ltd.

Cerium Oxide Nanoparticles Protect Endothelial Cells from Apoptosis Induced by Oxidative Stress
Co-reporter:Shizhu Chen;Yingjian Hou;Gong Cheng;Cuimiao Zhang
Biological Trace Element Research 2013 Volume 154( Issue 1) pp:156-166
Publication Date(Web):2013 July
DOI:10.1007/s12011-013-9678-8
Oxidative stress is well documented to cause injury to endothelial cells (ECs), which in turn trigger cardiovascular diseases. Previous studies revealed that cerium oxide nanoparticles (nanoceria) had antioxidant property, but the protective effect of nanoceria on ROS injury to ECs and cardiovascular diseases has not been reported. In the current study, we investigated the protective effect and underlying mechanisms of nanoceria on oxidative injury to ECs. The cell viability, lactate dehydrogenase release, cellular uptake, intracellular localization and reactive oxygen species (ROS) levels, endocytosis mechanism, cell apoptosis, and mitochondrial membrane potential were performed. The results indicated that nanoceria had no cytotoxicity on ECs but had the ability to prevent injury by H2O2. Nanoceria could be uptaken into ECs through caveolae- and clathrin-mediated endocytosis and distributed throughout the cytoplasma. The internalized nanoceria effectively attenuated ROS overproduction induced by H2O2. Apoptosis was also alleviated greatly by nanoceria pretreatment. These results may be helpful for more rational application of nanoceria in biomedical fields in the future.
Synthesis, characterization and cytotoxicity of gold(III) complexes with deprotonated pyridyl carboxamide
Co-reporter:Shuxiang Wang;Yanan Shi;Wenqing Shao;Jianlong Du;Haiying Wei;Jinchao Zhang;Ke Wang;Shigang Shen;Shenghui Li;Jitai Li;Yangyang Zhao
Applied Organometallic Chemistry 2012 Volume 26( Issue 10) pp:504-510
Publication Date(Web):
DOI:10.1002/aoc.2888

Six new gold(III) complexes [Au(bzpam)Cl2] (1, bzpamH = N-benzyl picolinamide), [Au(hetpam)Cl2] (2, hetpamH = N-(2-hydroxyethyl) picolinamide), [Au(pypam)Cl]AuCl4 (3, pypamH = N-(pyridin-2-ylmethyl) picolinamide), [Au(dmepam)Cl]AuCl4 (4, dmepamH = N-(2-(dimethylamino)ethyl) picolinamide), [Au(bhetpydam)Cl] (5, bhetpydamH2 = N,N′-bis(2-hydroxyethyl) pyridine- 2,6-dicarboxamide) and [Au2(hedam)Cl4] (6, hedamH2 = N,N′-(hexane-1,6-diyl) dipicolinamide) with deprotonated pyridyl carboxamide were synthesized and characterized by elemental analysis, molar conductivity, IR, H1 NMR and C13 NMR techniques. The analytical data showed that deprotonated pyridyl carboxamide coordinated with gold(III) ions through a nitrogen atom. The cytotoxicity against Bel-7402 and HL-60 cell lines was tested by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and SRB (sulforhodamine B) assays. The results indicated that the complexes exerted cytotoxic effects against Bel-7402 and HL-60 cell lines, complex 6 had better cytotoxicity than cisplatin, and complex 3 displayed similar cytotoxicity to cisplatin against Bel-7402 cell line. The results suggested that the characteristics of ligands had an important effect on cytotoxicity of complexes. Copyright © 2012 John Wiley & Sons, Ltd.

Synthesis, characterization and cytotoxicity of the gold(III) complexes of 4,5-dihydropyrazole-1-carbothioamide derivatives
Co-reporter:Shuxiang Wang, Wenqing Shao, Hongdong Li, Cui Liu, Ke Wang, Jinchao Zhang
European Journal of Medicinal Chemistry 2011 Volume 46(Issue 5) pp:1914-1918
Publication Date(Web):May 2011
DOI:10.1016/j.ejmech.2011.02.031
Eight new gold(III) complexes (1–8) of 5-aryl-3-(pyridin-2-yl)-4,5-dihydropyrazole-1-carbothioamide have been synthesized and characterized by elemental analysis, molar conductivity, IR, UV, 1H NMR, 13C NMR, MS, and thermal analysis techniques. The cytotoxicity was tested by MTT assay. The results indicate that the complexes 1–8 exert cytotoxic effects against HeLa and A549 cell lines. Moreover, the complexes 1, 4, 5, 7 and 8 have higher cytotoxicity than cisplatin against HeLa cell line. It suggests that the substituent groups on benzene have important effect on cytotoxicity.Eight new gold(III) complexes have been synthesized and characterized. The cytotoxicity was tested by MTT assay.Highlights► Eight new gold(III) complexes have been synthesized. ► Some complexes have higher cytotoxicity than cisplatin against HeLa cell line. ► The substituent groups on benzene have effect on cytotoxicity.
A novel anticancer theranostic pro-prodrug based on hypoxia and photo sequential control
Co-reporter:Weipei Feng, Chunyue Gao, Wei Liu, Huihui Ren, Chao Wang, Kun Ge, Shenghui Li, Guoqiang Zhou, Hongyan Li, Shuxiang Wang, Guang Jia, Zhenhua Li and Jinchao Zhang
Chemical Communications 2016 - vol. 52(Issue 60) pp:NaN9437-9437
Publication Date(Web):2016/06/23
DOI:10.1039/C6CC02932A
A novel anticancer pro-prodrug (GMC-CAE-NO2) with diagnosis and therapy functions based on hypoxia and photo sequential control was designed. It provides a platform for constructing theranostic pro-prodrugs to release active drugs controlled by hypoxic status and UV illumination.
A novel dual-emission fluorescent probe for the simultaneous detection of H2S and GSH
Co-reporter:H. Li, W. Peng, W. Feng, Y. Wang, G. Chen, S. Wang, S. Li, H. Li, K. Wang and J. Zhang
Chemical Communications 2016 - vol. 52(Issue 25) pp:NaN4631-4631
Publication Date(Web):2016/02/26
DOI:10.1039/C6CC00973E
A novel chlorinated coumarin–malononitrile fluorescent probe with three potential reaction sites, which exhibited highly selective, rapid response, low detection limit, and was capable of the simultaneous detection of H2S (λex/em = 515/564 nm) and GSH (λex/em = 430/517 nm), was first proposed. The probe was successfully applied to dual-channel imaging H2S and GSH in MCF-7 cells.
1H-Pyrazole, 4-[4-(trifluoromethyl)phenyl]-
1H-Pyrazole, 4-[4-(trifluoromethyl)phenyl]-
CAS:497946-98-2
1H-Pyrazole, 4-[4-(trifluoromethyl)phenyl]-
Dipyrido[3,2-a:2',3'-c]phenazine, 10,11,12,13-tetrahydro-
Dipyrido[3,2-a:2',3'-c]phenazine, 10,11,12,13-tetrahydro-
CAS:208845-21-0
Dipyrido[3,2-a:2',3'-c]phenazine, 10,11,12,13-tetrahydro-
CAS:182344-65-6
Caspase-3
Caspase-3
CAS:169592-56-7
Caspase-3
EPTAPLATIN
EPTAPLATIN
CAS:146665-77-2
EPTAPLATIN
Protein kinase A
Protein kinase A
CAS:142008-29-5
Protein kinase A
PLATINUM, [REL-(1R,2R)-1,2-CYCLOBUTANEDIMETHANAMINE-.KAPPA.N,.KAPPA.N'][(2S)-2-(HYDROXY-.KAPPA.O)PROPANOATO(2-)-.KAPPA.O]-, (SP-4-3)- (9CI) (CA INDEX NAME)
PLATINUM, [REL-(1R,2R)-1,2-CYCLOBUTANEDIMETHANAMINE-.KAPPA.N,.KAPPA.N'][(2S)-2-(HYDROXY-.KAPPA.O)PROPANOATO(2-)-.KAPPA.O]-, (SP-4-3)- (9CI) (CA INDEX NAME)
CAS:135558-11-1
PLATINUM, [REL-(1R,2R)-1,2-CYCLOBUTANEDIMETHANAMINE-.KAPPA.N,.KAPPA.N'][(2S)-2-(HYDROXY-.KAPPA.O)PROPANOATO(2-)-.KAPPA.O]-, (SP-4-3)- (9CI) (CA INDEX NAME)
Nedaplatin
Nedaplatin
CAS:95734-82-0
Nedaplatin
Sodium yttrium fluoride
Sodium yttrium fluoride
CAS:65453-57-8
Sodium yttrium fluoride
CAS:34235-81-9