Co-reporter: Zhiyong U. Wang, Yane-Shih Wang, Pei-Jing Pai, William K. Russell, David H. Russell, and Wenshe R. Liu
pp:
Publication Date(Web):June 14, 2012
DOI: 10.1021/bi300535a
Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.
