Co-reporter: Benjamin P. Warlick, Bradley S. Evans, Tobias J. Erb, Udipi A. Ramagopal, Jaya Sriram, Heidi J. Imker, J. Michael Sauder, Jeffrey B. Bonanno, Stephen K. Burley, F. Robert Tabita, Steven C. Almo, Jonathan S. Sweedler, and John A. Gerlt
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Publication Date(Web):October 4, 2012
DOI: 10.1021/bi301215g
Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.
